• Journal of Plant Biotechnology
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• 제49권3호
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• pp.222-229
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• 2022

사과는 국내 과수산업에서 가장 많이 재배되고 있는 과종이다. 하지만 apple mosaic virus (ApMV), apple stem grooving capillovirus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple scar skin viroid (ASSVd)와 같은 바이러스 및 바이로이드에 감염되면 과실의 수확량 감소 및 품질 저하를 야기시킨다. 본 연구에서는 국내 사과 농가에서 가장 많이 감염되어 있는 ASGV 바이러스를 제거하기 위한 효율적인 무병화 시스템을 확립하고자 하였다. ASGV에 감염된 폿트묘를 36℃, 38℃, 40℃가 유지되는 항온·항습장치에서 4주간 열처리를 수행하였으며, 신초 생장율과 바이러스 제거율을 조사하였다. 신초 생장률은 36℃ 처리구에서 가장 높았으며 신초의 중간부와 상단부는 바이러스가 제거되었으나 하단부는 바이러스가 제거되지 않았다. 38℃, 40℃ 처리구는 신초의 모든 구간에서 바이러스가 제거되지 않았으며, 40℃ 처리구는 신초의 생장 없이 열처리 3주 후 고사되었다. 36℃ 온도에서 열처리된 폿트묘의 경정을 절취하여 기외에서 접목하였으며 94%의 생존율과 20%의 바이러스 제거율을 보였다. 따라서 열처리 및 경정접목을 통해 무병묘 생산이 가능할 것으로 판단되었다.

• 농업과학연구
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• 제43권1호
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• pp.21-27
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• 2016

Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these 'S' isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.

• 한국산업융합학회 논문집
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• 제19권4호
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• pp.237-244
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• 2016

This paper investigates the possibility to increase the economic value of the thinned young apple trough fermentation process. It is studies the optimal fermentation condition of thinned young apple by manipulating preparation treatment and sugar content. To do this following steps are done. Firstly, different preparation treatment for thinned young apple such as whole apple, cutting apple, and blending apple were done. The different sugar content such as $24^{\circ}Brix$, $15^{\circ}Brix$ and $4^{\circ}Brix$ was added. Secondly, the sugar contents and pH level were measured during fermentation process. Finally, statistical analysis was used to examine the relation between the preparation treatment, sugar content and pH level during the fermentation process. The experimental result shows that the different preparation treatment influences to the fermentation process. The blending apple treatment gave lower pH level compared to other kind treatments. The sugar content of thinned young apple was decrease during the fermentation process. The statistical analysis shows that the manipulation preparation treatment and sugar content affect the final pH level and whole fermentation process. Experimental result shows that the thinned young apple fermentation could be proposed as new alternative product in the market. The best fermentation process was obtained from blending thinned young apple treatment with $24^{\circ}Brix$.

• 한국조리학회지
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• 제23권8호
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• pp.98-105
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• 2017

The purpose of this study was to investigate changes in physicochemical properties of apples pre-treated differently with salt and sugar for apple Jangachi. When salt was used, moisture content was decreased by 28.41% to 57.67% at 24 hours and maintained an average 56.92% after 24 hours. However, when sugar was used, moisture content decreased steadily to 41.14% (60h). The pH of the apple pre-treated with salt decreased from pH 4.42 to pH 3.63 at 12 hours. However, in the case of apples pre-treated with sugar, pH decreased from pH 4.52 to pH 4.19 after 48 hours, but was not statistically significant. Conversely, total acidity of apple pre-treated with salt increased from 11.46% to 0.35% during 72 hours. But total acidity of apple pre-treated with sugar decreased to 0.11% at 24 hours and maintained. Sugar content of apple pre-treated with salt increased to 33.1% at 12 hours and maintained. Conversely, in case of sugar pre-treatment, sugar content of apple pre-treated with sugar increased steadily to 45.12% at 72 hours. Salinity of apple pre-treated with salt increased sharply to 15.74% during 24 hours. Lightness ($L^*$) of apple pre-treated sugar was not different from the control group. But apple pre-treated salt decreased slightly. Yellowness ($b^*$) was higher than the control group regardless of pre-treatment group. Sensory evaluation revealed that sugar pre-treatment apples were highly evaluated for flavor, taste, chewiness and overall acceptance.

• 한국식품과학회지
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• 제31권2호
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• pp.375-379
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• 1999

식품공정에 있어서 비가열 살균기술로 개발되고 있는 고전압 펄스 전기장(PEF)을 사과주스에 적용하여 실용성 있는 비가열 처리 신선 사과주스의 개발 가능성을 검토하고자 하였다. 이를 위하여 PEF와 가열처리한 사과주스를 저장하면서 비타민 C, 색깔, 향기성분, 관능평가 등 품질특성 변화를 조사하였다. PEF 처리와 가열 처리한 사과주스의 $5^{\circ}C$ 저장중 색깔은 큰 차이가 없었으나 비타민 C 함량은 PEF처리한 사과주스가 가열처리한 사과주스에 비하여 높은 함량을 나타내었으며, 신선 주스와 거의 같은 함량을 보였다. 향기성분도 대조구에 비하여 가열처리한 사과주스는 약 70% 이상의 심한 향기성분의 손실을 나타낸 반면 PEF처리한 사과주스는 약 15%의 향기성분의 손실만을 나타내었다. 관능적 기호도도 PEF 처리한 사과주스는 신선 사과주스와 차이가 없었으나, 가열처리한 사과주스에 비하여 유의적으로 우수한 것으로 나타났다(P<0.05). PEF 처리 사과주스는 $5^{\circ}C$에서 30일간의 저장기간 동안 품질 변화가 거의 없었다.

• 인터넷정보학회논문지
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• 제11권6호
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• pp.135-144
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• 2010

최근 발표되었던 폐쇄적인 Apple의 새로운 Software Development Kit(SDK) 정책으로 인해 Adobe가 자사의 소프트웨어를 사용한 AppStore의 애플리케이션 개발이 불가능해지자 반독점행위라고 주장하게 되었고 Department of Justice(DOJ)와 Fair Trade Commission(FTC)에 반독점행위 여부를 조사해 줄 것을 요청했던 바 있었다. 1998년에 있었던 Microsoft의 반독점 소송과 비교하면서 Apple이 스마트폰 시장을 독점하려 한다는 주장도 있으나 본질적인 내용에 있어서 분명한 차이가 있으며 과연 Apple의 선택적인 iPhone과 iPad용 애플리케이션 개발프로그램 지정이 반독점적인 상황인지는 따져볼 필요가 있다. 스마트폰 시장의 양면시장적 성격 때문에 시장획정을 어떻게 하든 Apple의 iPhone이 휴대전화시장 또는 스마트폰 시장에서 독과점적 지위를 갖지는 않을 수 있지만 애플리케이션 스토어 시장에서 시장지배적 지위를 갖는 것으로 간주할 수도 있기 때문에 반독점적 성격이 전혀 없다고 보기도 어려운 상황이었다. 그러나 지난 9월 Apple은 자발적으로 SDK개방정책을 새롭게 발표하게 되었고 이는 시장에서 상당히 긍정적인 결과로 받아들여지고 있다. DOJ, FTC 등 미국의 규제기관들이 성급하게 끼어들기 보다는 시간을 두고 사업자들의 상호작용을 지켜보면서 시장실패가 존재하는지를 파악하고 소비자의 이해관계에 해가 되는지를 면밀하게 검토하는 신중함을 보인 부분에 대해서는 상당히 바람직하다고 할 수 있겠다. 무엇보다 중요한 것은 Adobe가 소비자와 개발자들의 선택의 자유를 표방하면서 Apple을 공격하고 있지만 객관적인 관점에서 Apple이 반독점적 행위를 하고 있는지, 그 행위에 의한 피해를 플랫폼의 양쪽에 있는 개발자와 소비자들이 겪게 되어 시장의 실패가 존재하는지에 대한 종합적 분석에 근거한 결론이 필요하다.

• 한국식품저장유통학회지
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• 제19권2호
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• pp.178-184
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• 2012

본 연구에서는 살균 온도에 따른 사과주스의 이화학적 특성과 영양성분 및 기능성을 분석하였다. pH는 저온살균 사과주스가 고온살균 사과주스보다 약간 높았으며, 적정산도는 고온살균 사과주스가 저온살균 사과주스보다 높았다. 두 시료의 가용성 고형분 함량은 동일하였으며, 색도는 고온살균 사과주스의 명도가 높았으며, 황색도와 적색도의 경우는 저온살균 사과주스가 약간 더 높게 나타났다. 환원당 함량은 고온살균 사과주스가 저온살균 사과주스보다 높은 경향을 나타냈다. 두 시료 모두 칼륨과 malic acid 함량이 가장 많았다. 유리 아미노산의 총 함량은 고온살균 사과주스가 높았으며, aspartic acid와 glutamic acid의 함량이 그 중 가장 높게 나타났다. 총 폴리페놀 함량은 저온살균 사과주스가 고온살균 사과주스 보다 유의적으로 높았으며, 라디칼 소거능에서는 DPPH 라디칼 소거능은 저온살균 사과주스가 고온살균 주스에 비해 유의적으로 높았으며, xanthine oxidase 저해 활성 및 tyrosinase 저해 활성에서 저온살균 사과주스가 고온살균 사과주스에 비해 높게 나타났다. 이상의 결과, 사과주스 제조시 고온살균을 할 경우 농축으로 인해 일반성분의 함량이 다소 높게 나타날 수 있으나, 사과의 기능성을 유지하기 위해서는 저온살균 방법이 고온살균 방법에 비해 효과적일 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1192-1200
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• 2006

An apple polygalacturonase-inhibiting protein (PGIP), which specifically inhibits endopolygalacturonase (PG, EC 3.2.1.15) from Botryosphaeria dothidea, was purified from Botryosphaeria dothidea-infected apple (Malus domestica cv. Fuji) fruits. The purified apple PGIP had a molecular mass of 40 kDa. The N-terminal amino acid sequence of the purified protein showed high homologies to those of PGIP from pear (100%), tomato (70%), and bean (65%). We also purified polygalacturonase (PG) from B. dothidea. The PG hydrolyzes pectic components of plant cell walls. When the extracted apple pectic cell wall material was treated with purified apple PGIP and B. dothidea PG, the amount of uronic acid released was lower than that treated with B. dothidea PG alone. This result demonstrates that PGIP functions specifically by inhibiting cell wall maceration of B. dothidea PG Furthermore, we characterized the de novo function of the PGIP against PG on the solubilization and depolymerization of polyuronides from cell wall of apple fruits inoculated with B. dothidea. This result demonstrated that the PGIP of plants exhibits one of the direct defense mechanisms against pathogen attack by inhibiting PGs that are released from pathogens for hydrolysis of cell wall components of plants.

• The Plant Pathology Journal
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• 제22권2호
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• pp.168-173
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• 2006

To develop the diagnostic method for the viral infection in apple, the partial genes corresponding to the N-terminal region of RNA polymerase of Apple stem grooving virus (ASGV) and coat protein of Apple chlorotic leaf spot virus (ACLSV) were characterized from the infected apple cultivars in Korea. Based on the nucleotide sequences of the characterized partial genes, the virus gene-specific primers were designed for the detection of ASGV and ACLSV infected in species of Malus. The RT-PCR using the primers for the genes of ASGV and ACLSV successfully gave rise to 404 and 566 bp DNA fragments, respectively. Using those viral gene-specific primers, the multiplex RT-PCR assays were also established to diagnose the mixed infection by ASGV and ACLSV simultaneously. Furthermore, the control primers, which have to be included for the RT-PCR as an internal control, were designed using the nucleotide sequence of the gene encoding elongation factor $1{\alpha}(EF1{\alpha})$. This multiplex RT-PCR including the control primers provides more reliable, rapid and sensitive assay for the detection of ASGV and ACLSV infected in Korean apple cultivars.

• Journal of Biosystems Engineering
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• 제16권3호
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• pp.272-280
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• 1991

The aims of this study were to investigate the feasivility of analyzing a few sorting factors such as size, coloration and defect of apples with a monochrome image processing system and to find apparent properties which could be effectively used for apple sorting. The results are summarized as follows. 1. A computer program was made to analyze the projection area, coloration and defect of an apple with a monochrome image processing system. 2. The algorithm developed to compute the projedtion area of an apple was between on the proportional relation between a given reference area and the corresponding number of pixels, and the computing time was 0.74 to 0.82 second depending on the size of apple. 3. The coloration of an apple was expressed as the ratio of the gray value of a reference color to that of a given bounded area of the stem end surface (defined as coloration index), and the computing time was about 3.0 seconds with this algorithm. 4. Defect of an apple could be isolated by lowpass filtering and image subtraction but it took about 20 seconds in computing time. 5. The coloration of the Fuji apple could be classified into 3 to 4 groups by the coloration index and also, it was found that the correlation coefficient between the indices and sugar contents was 0.74. 6. The coloration index obtained from a given bounded area of the stem end side of the Fuji apple could represent the coloration of total surface with a correlation coefficient of 0.922.