• Research in Plant Disease
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• v.17 no.3
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• pp.396-398
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• 2011

Soft rot disease on Raspberry (Rubus crataegifolius Bunge) was observed in sale boxes at Jinju City Wholesale Market of Agricultural Products in June 2010. The infected fruits were rapidly water-soaked, softened, black and eventually rotted. The colonies on the infected fruits were white to light brown, formed numerous sporangiospores. Optimum temperature for the mycelial growth of the causal fungus on PDA was $30^{\circ}C$ and growth was still apparent at $37^{\circ}C$. Sporangia were globose, white at early and gradually to black, and 40-210 ${\mu}m$ in diameter. Sporangiophores were white to mid brown as maturation and 8-20 ${\mu}m$ in diameter. Columella were globose to sub-globose, and the size of the diameter was 85-120 ${\mu}m$ in diameter. Sporangiospores were sub-globose, rhomboidal and irregular, streaked and 5-10 ${\mu}m$ in length. On the basis of symptom, mycological characteristics, ITS rDNA sequence analysis, and pathogenicity of the fungus, the causal fungus was clearly identified as Rhizopus oryzae Went & Prinsen Geerligs. This is the first report of soft rot by R. oryzae on R. crataegifolius in Korea.

• Journal of the Korean Ceramic Society
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• v.42 no.1
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• pp.62-68
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• 2005

Oxidtion behavior of $Si_{3}N_{4}$ ceramics with the different porosity by the Nitrided Pressureless Sintering (NPS) were investigated in pure oxygen gas atmosphere at 1000 to $1300^{circ}C$. The thickness of formed oxide film on the surface of silicon nitride ceramics was increased with oxidation time and temperature. The oxide film thickness of 5A5Y5Si and 5A5Y10Si specimens for 100 h at 1300^{circ}C$ was about 10 $\mu$m and 20 $\mu$m, respectively. The oxidation of 5A5Y5Si and 5A5Y10Si specimens follows the parabolic behavior with an apparent activation energy of 215 kJ/mol and 104 kJ/mol, respectively. The flexural strength of 5A5Y5Si specimens after oxidation test for 500 h at 1300^{circ}C were maintained as-received value of 500 ma. On the other hand, that of 5A5Y10Si specimens were decreased about 100 MPa in as-received value.

• Ocean and Polar Research
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• v.33 no.4
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• pp.435-445
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• 2011

We investigated geochemical properties, and microbial sulfate- and iron(III) reduction in sediment influenced by the aquaculture of abalone in the Nohwa-do, southern coastal sea in Korea. Concentrations of ammonium, phosphate, and sulfide in the pore-water were higher at farm sites than at control sites. The differences between the 2 types of sites were most apparent in terms of the weights of abalone and the temperature increase during September. Accordingly, the rates of sulfate reduction at the farm sites during September (61 mmol S $m^{-2}d{-1}$) were 3-fold higher than the sulfate reduction during May (20 mmol S $m^{-2}d{-1}$). In contrast, Fe(III) reduction rates were highest at the control sites in May, but its significance was relatively decreased at farm sites during September when sulfate reduction rates were highest. During September, benthic ammonium flux was 3-fold greater at the farm sites (35 mmol N $m^{-2}d{-1}$) than at the control sites (12 mmol N $m^{-2}d{-1}$), and phosphate flux was 8-fold higher at the farm sites (0.018 mmol P $m^{-2}d{-1}$) than at control sites (0.003 mmol P $m^{-2}d{-1}$). Overall results indicated that the inappropriate operation of a large-scale aquaculture farm may result in excess input of biodeposits and high nutrient fluxes from the sediment, thereby decreasing diversity of the benthic ecosystem and deepening eutrophication in coastal waters.

• Journal of Animal Science and Technology
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• v.61 no.6
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• pp.313-323
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• 2019

The interactive effect of dietary fat supplementation and milk yield level on dairy cows performance under heat stress has not been thoroughly investigated. The purpose of this study was to evaluate the effect of production level, the source of fat supplements and their interaction on dairy cows performance under heat stress. In this study, 64 Holstein multiparous cows were divided into 2 groups and received one of two rations having either calcium salts of fatty acids (Ca-FA) or high-palmitic acid (PA) supplements (2.8% of DM; dry matter). After completing the experiment and based on maturity-equivalent milk, cows were divided into two groups of high-yielding (14,633 kg) and medium-yielding (11,616 kg). Average temperature humidity index (THI) was 71 during the trial period. Apparent digestibility of dry matter (p = 0.04), organic matter (p = 0.05), and neutral detergent fiber (NDF; p = 0.04) for cows fed Ca-FA were greater than cows fed PA. The milk fat content in high-producing cows was 0.3% greater than medium-producing cows (p = 0.03). The milk protein content in cows fed Ca-FA was greater than cows fed PA (p < 0.01). High-producing cows had greater serum cholesterol (p = 0.02) than medium-producing cows. The cows fed PA tended to have a greater BUN than cows fed Ca-FA (p = 0.06). Alanine aminotransferase and aspartate aminotransferase tended to be increased by PA, which indicates that cows in PA treatment may have experienced more adverse effect on the liver function than cows on Ca-FA. Therefore, under heat stress and in 90 d trial, milk production level does not affect the cows' response to PA or Ca-FA. Although cows fed Ca-FA received lower energy than those fed PA, they compensated for this shortage likely with increasing the digestibility and produced a similar amount of milk.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.6 no.3
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• pp.163-170
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• 2008

The reductive stripping of Np using a n-butyraldehyde (NBA) from loaded organic solution containing Np, which was oxidative-extracted in a system of a 30 % TBP/NDD-2M $HNO_3$ and O/A=2 containing 0.005 M $K_2Cr_2O_7$ as an oxidant of Np, was studied. The stripping yields of Np was increased with an increasing the NBA concentration, with a decreasing the nitric acid concentration of stripping solution and with a decreasing the reaction temperature. The apparent reductive stripping rate equation was shown by the following equation : $-d[Np]_{Org.}/dt$ = 1,524 exp(-2,906/T) $[NBA]^{0.91}\;[H^+]^{-0.92}[Np]_{Org.}$. At 1.04 M NBA and 2 M $NHO_3$, the stripping yield of Np and U was 70.1 %, and 7.1 %, respectively, and the separation factor of U over Np ($=D_U/D_{Mp}$) was about 30.4. Therefore, it was found that U and Np co-extracted in a system of TBP-$HNO_3$ could be effectively mutual-separated by the NBA.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.987-996
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• 2009

Aspergillus niger GH1 previously isolated and identified by our group as a wild tannase producer was grown under solid-state (SSC) and submerged culture (SmC) conditions to select the enzyme production system. For tannase purification, extracellular tannase was produced under SSC using polyurethane foam as the inert support. Tannase was purified to apparent homogeneity by ultrafiltration, anion-exchange chromatography, and gel filtration that led to a purified enzyme with a specific activity of 238.14 IU/mg protein with a final yield of 0.3% and a purification fold of 46. Three bands were found on the SDS-PAG with molecular masses of 50, 75, and 100 kDa. PI of 3.5 and 7.1% N-glycosylation were noted. Temperature and pH optima were 600e and 6.0 [methyl 3,4,5-trihydroxybenzoate (MTB) as substrate], respectively. Tannase was found with a $K_M$ value of $0.41{\times}10^{-4}M$ and the value of $V_{max}$ was $11.03{\mu}$moL/min at $60^{\circ}C$ for MTB. Effects of several metal salts, solvents, surfactants, and typical enzyme inhibitors on tannase activity were evaluated to establish the novelty of the enzyme. Finally, the tannase from A. niger GH1 was significantly inhibited by PMSF (phenylmethylsulfonyl fluoride), and therefore, it is possible to consider the presence of a serine or cysteine residue in the catalytic site.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.907-916
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• 2013

The aim of this study was to compare community structure of larval fish species in the northern East China Sea during normal meteorological conditions in autumn 2009, during the El Ni$\tilde{n}$o period in 2009-2010, and during the La Nina period in 2010. Fifty taxa were recorded during the study period; the most dominant species were Benthosema pterotum and Gobiidae spp. In October 2008 during the normal period, warm water from the Tsushima Warm Current (TWC) intruded more into the surface and middle layers, and cold water affected by the Yellow Sea Cold Water (YSCW) intruded into the bottom layer. In October 2009 during the El Ni$\tilde{n}$o period, intrusion of the China Coastal Water (CCW), which has low salinity (<32.2 psu), was more apparent than intrusion of the TWC or YSCW. In October 2010 during the La Nina period, intrusion of the TWC and CCW was relatively weak, resulting in the lowest temperature and highest salinity observed during the study period in the eastern part of the study area. Hierarchical cluster, one-way ANOSIM (analysis of similarities), and SIMPER (similarity-percentages procedure) analyses provided two main results. First, the abundance of the most dominant larval fish species in autumn of the normal period was greater than that in autumn of the El Ni$\tilde{n}$o/La Nina periods, resulting in a significant difference in ichthyoplankton community structure between the periods. The abundance of Benthosema pterotum increased in the normal period, possibly influenced by the intrusion of cold water from the YSCW; the abundance of species residing in Korean waters (e.g., Gobiidae spp.) probably decreased during the El Ni$\tilde{n}$o/La Nina periods. The second finding was that the abundance of subtropical larval fish in autumn of the normal period was generally larger than that during autumn of the El Ni$\tilde{n}$o/La Nina periods. This could have been induced by the stronger intrusion of warm water from the TWC during the normal period. Although differences in oceanographic conditions between El Ni$\tilde{n}$o and La Nina periods were observed, the differences in ichthyoplankton community structure between the two periods were not significant.

• Microbiology and Biotechnology Letters
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• v.34 no.4
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• pp.311-317
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• 2006

Using PCR amplification, we cloned a cellulase gene (ce/H) from the Bacillus subtilis AH18 which has plant growth-promoting activity and antagonistic ability against pepper blight caused by Phytophthora capsici. The 1.6 kb PCR fragment contained the full sequence of the cellulase gene and the 1,582 bp gene deduced a 508 amino acid sequence. Similarity search in protein database revealed that the cellulase of B. subtilis AH18 was more than 98% homologous in the amino acid sequence to those of several major Bacillus spp. The ce/H was expressed in E. coli under an IPTG inducible lac promoter on the vector, had apparent molecular weight of about 55 kDa upon CMC-SDS-PAGE analysis. Partially purified cellulase had not only cellulolytic activity toward carboxymethyl-cellulose (CMC) but also insoluble cellulose, such as Avicel and filter paper (Whatman No. 1). In addition, the cellulase could degrade a fungal cell wall of Phytophthora capsici. The optimum pH and temperature of the ce/H coded cellulase were determined to be pH 5.0 and $50^{\circ}C$. The enzyme activity was activated by $AgNO_3$ or $CoCl_2$. However its activity was Inhibited by $HgC1_2$. The enzyme activity was activated by hydroxy urea or sodium azide and inhibited by CDTA or EDTA. The results indicate that the cellulase gene, ce/H is an antifungal mechanism of B. subtilis AH18 against phytophthora blight disease in red-pepper.

• The Korean Journal of Physiology
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• v.24 no.1
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• pp.51-65
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• 1990

The purpose of the present study was to examine the hemodynamic responses, especially in arterial and skin blood flows, in conjunction with the changes of plasma catecholamine levels as an indirect marker of adrenergic tone during the early stage of head-down tilt (HDT), and to evaluate the early physiological regulatory mechanism in simulated weightlessness. Ten mongrel dogs, weighing8\;{\sim}\;14\;kg, were intravenously anesthetized with nembutal, and postural changes were performed by using the tilting table. The postural changes were performed in the following order: supine, prone, HDT $(-6^{\circ}C)$ and lastly recovery prone position. The duration of each position was 30 minutes. The measurements were made before, during and after each postural change. The arterial blood flow $({\.{Q}})$ at the left common carotid and right brachial arteries was measured by the electromagnetic flowmeter. Blood pressure (BP) was directly measured by pressure transducer in the left brachial artery. To evaluate the peripheral blood flow, skin blood flow $({\.{Q}})$ was calculated by the percent changes of photoelectric pulse amplitude on the forepaw, and skin temperature was recorded. The peripheral vascular resistance (PR) was calculated by dividing respective mean BP values by ${\.{Q}}$ of both sides of common carotid and brachial arteries. Heart rate (HR), respiratory rate (f) and PH, $Po_{2},\;Pco_{2}$ and hematocrit of arterial and venous blood were also measured. The concentration of plasma epinephrine and norepinephrine was measured by radioenzymatic method. The results are summarized as follows: Tilting to head-down position from prone position, HR was initially increased (p<0.05) and BP was not significantly changed. While ${\.{Q}}$ of the common carotid artery was decreased (p<0.05) and PR through the head was increased, ${\.{Q}}$ of the brachial artery was increased (p<0.05) and PR through forelimbs was decreased. ${\.{Q}}$ of the forepaw was initially increased (p<0.05) and then slightly decreased, on the whole revealing an increasing trend. Plasma norepinephrine was slightly decreased and the epinephrine was slightly increased. f was increased and arterial pH was increased (p<0.05). In conclusion, the central blood pooling during HDT shows an increased HR via Bainbridge reflex and an increased ${\.{Q}}$ of the forepaw and brachial ${\.{Q}}$, due to decreased PR which may be originated from the depressor reflex of cardiopulmonary baroreceptors. It is suggested that the blood flow to the brain was adequately regulated throughout HDT $(-6^{\circ}C)$ in spite of central blood pooling. And it is apparent that the changes of plasma norepinephrine level are inversely proportional to those of ${\.{Q}}$ of the forepaw, and the changes of epinephrine level are paralleled with those of the brachial ${\.{Q}}$.

• Korean Chemical Engineering Research
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• v.48 no.1
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• pp.80-87
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• 2010

Lignite of low rank coal and petroleum coke of high sulfur content can be high potential energy sources for coal gasification process because of their plentiful supply. The kinetic study of steam gasification has been performed in an atmospheric thermobalance with wood chip, lignite, bituminous, anthracite, pet-coke. The effects of gasification temperature($600{\sim}850^{\circ}C$) and partial pressure of steam(30~90 kPa) on the gasification rate have been investigated. The modified volumetric reaction model was applied to the experimental data to describe the behavior of carbon conversion and to evaluate the needed kinetic parameters. Lignite and wood chip with high volatile content showed high average gasification rates comparing to other fuel and thus they might be proper fuel for gasification processes. The activation energies for wood chip, lignite, bituminous, anthracite, and pet-coke through Arrhenius plot were found to be 260.3, 167.9, 134.6, 82.2, 168.9 kJ/mol, respectively. The expression of apparent reaction rates for steam gasification of various chars have been proposed as basic information for the design of coal gasification processes.