• Clinical and Experimental Reproductive Medicine
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• v.26 no.2
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• pp.225-230
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• 1999

In mouse, the heat shock protein 70-2 (hsp70-2) is found to have special function in spermatogenesis. Based on the observation, the hypothesis that human hspA2 (human gene; 98.2% amino acid homology with hsp70-2) might have important function in spermatogenesis in human testes was proposed. To test the hypothesis, we examined the expression of hspA2 in human tissues. Expression vector pDMC4 for expression of the human hspA2 protein using pTricHisB (invitrogen, USA) was constructed and the expressed hspA2 protein was cross-reacted with antiserum 2A raised against mouse hsp70-2 protein. Based on the cross-reactivity, we determined the expression level of hspA2 protein in human tissues by western blot analysis using the antiserum 2A. We demonstrated that antiserum 2A antibodies detected human hspA2 protein with specificity which was produced in the E.coli expression system. On Western blot analyses, significant hspA2 expression was observed in testes with normal spermatogenesis, whereas a low level of hspA2 was expressed in testis with Sertoli-cell only syndrome. Also, a small amount of hspA2 was detected in breast, stomach, prostate, colon, liver, ovary, and epididymis. These results demonstrate that the hspA2 protein is highly expressed in male specific germ cells, which in turn suggests that hspA2 protein might playa specific role during meiosis in human testes as suggested in the murine model. However, further studies should be attempted to determine the function of hspA2 protein in human spermatogenesis.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.1
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• pp.43-47
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• 1983

Tobacco mosaic virus (TMV) was detected from Kimchi by biological and serological assay 5. Kimchi samples three month after cooked were collected, and were inoculated on N. tabacum var. Burley 21 and NC 95. Out of 33 samples, 6 showed typical symptoms induced by TMV, local necrotic lesions on Burley 21 and mosaic on NC 95. All saps from tobacco leaves showed the mosaic symptom reacted positively against TMV antiserum by agar gel double diffusion test. Based on the results, the Kimchi is considered as one of the important inoculum sources in Korea.

• Toxicological Research
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• v.4 no.2
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• pp.143-149
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• 1988

Cytolysin produced by Vibrio vulnificus ATCC 27562 was partially purified by sequential ammonium sulfate precipitation, gel filtration with Sephadex G-200, and ion exchange chromatography with DEAE-Sephadex. The partially purified cytolysin was inactivated by cholesterol. More than one molecule of the cytolysin was required to lyse a single erythrocyts. The antiserum against cytolysin enhanced the survival ratio of mice infected with low dose of V. vulnificus.

• Korean Journal of Microbiology
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• v.27 no.2
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• pp.162-167
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• 1989

Western blot analysis of lysates of Streptococcus pneumoniae revealed a single polypeptide species that cross-reacted with E. coli RecA antiserum. The apparent molecular weight of this putative RecA protein analog (RecAsp) was 24, 000 smaller than any other known RecA analogue. The RecAsp protein was present at the same level in competent and non-competent cells.

• YAKHAK HOEJI
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• v.16 no.2
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• pp.90-96
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• 1972

The author tried to isolate the Vibrio parahaemolyticus from Han River water and river fishes, to investigate the route of contamination by way of marine product and to make clear the survival ability in various kinds of food. The results are as follows: 1. Twenty eight strains of V. parahaemolyticus were identified among 312 samples in Han river from March to August. 2. V. parahaemolyticus was detected in the margenic content of Cyprinus and Anguilla. 3. 9 strains of K-3 type, 5 strains of K-11 type, 8 strains of K-8 type, 6 strains of K-32 type and 1 strain of K-52 type were clarified through K-antiserum reaction.

• Journal of Photoscience
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• v.9 no.2
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• pp.278-280
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• 2002

We have cloned a hydromedusan opsin cDNA and showed that the deduced amino acid sequence of the cytoplasmic loop between helices 5 and 6 (loop 5-6) was clearly different from that reported so far. The amino acid sequence of the loop 5-6 is important on determination of the specificity for the coupled G- protein. To clarify which class of G-protein mediates the phototransduction system in the ocellus of the hydromedusan, we investigated G-proteins expressed in the ocellus. By PCR against the cDNA of the ocellus with primers designed according to the conserved amino acid sequence in G-protein a subunit, we obtained three kinds of cDNA fragments. Based on the sequence similarities, ttwo of them (JGI and JG3) were classified as $G_{i}$ and $G_{q}$, respectively. The other one (JG2) was a new subtype within $G_{*}$ class. Electron microscopic immunocytochemistry with the antiserum against the C-terminal sequence of $G_{q}$ or $G_{t}$ revealed the presence. of the both classes in the ocellus. The similarity of the C-terminal sequence of the JG2 with that of bovine $G_{t}$ suggests that the anti- $G_{t}$ antiserum would bind to JG2. These results suggest the possibility that the hydromedusan rhodopsin decides the specificity for the coupled G-protein by the other domain than the loop 5-6.oop 5-6.5-6.

• Animal cells and systems
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• v.8 no.3
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• pp.213-220
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• 2004

MicroRNA and siRNA (small interfering RNA), representative members of small RNA, exert their effects on target gene expression through association with protein complexes called miRNP (microRNA associated ribonucleoproteins) and RISC (RNA induced silencing complex), respectively. Although the protein complexes are yet to be fully characterized, human EIF2C2 protein has been identified as a component of both miRNP and RISC. In this report, we raised antiserum against EIF2C2 in order to begin understanding the protein complexes. An immunoblot result indicates that EIF2C2 protein is ubiquitously expressed in a variety of cell lines from human and mouse. EIF2C2 protein exists in both cellular compartments, as indicated by an immunoblot assay with a nuclear extract and a cytosolic fraction (S100 fraction) from HeLa S3 lysate. Depletion of EIF2C1 or EIF2C2 protein resulted in a decrease of microRNA, suggesting a possible role of these proteins in microRNA stability or biogenesis. We also prepared antiserum against dsRNA binding protein PACT, whose homologs in C. elegans and Drosophila are known to have a role in the RNAi (RNA interference) pathway. The expression of PACT protein was also observed in a wide range of cell lines.

• Korean Journal of Animal Reproduction
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• v.10 no.2
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• pp.168-174
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• 1986

These experiments were carried out to clarify the optimalconditions and interspecific cross reaction of H-Y antibody activity. H-Y antiserum was prepared in inbred SD female rats and Balb/c female mice by repeated immunization of rat newborn testis homogemate, rat and mouse spleen cells obtained from males of same strain. The activity of H-Y antibody in antiserum was tested by ELISA and biological tests. The cross reactivity of H-Y antibody was confirmed by culturing mouse and rabbit embryos in medium containing H-Y antibody and complement obtained from rat and guinea pig, respectively. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos in medium with different pH and complement concentration. The results obtained in these experiments were summarized as follows: 1. The formation rates of H-Y antibody in rats immunized with newborn testis and spleen cell were 40.0 and 50.0% respectively, and that in mouse immunized with spleen cell was 48.4%. 2. The activity of H-Y antibody was not affected by pH in range of 6.5 to 8.0, and the same was true for the relative concentration of complement to the H-Y antibody. 3. Minimum time needed for the activity of H-Y antibody was confirmed to be 0.5 to 1 hour and 24 to 48 hours respectively for the zona free embryos and intact embryos. 4. When mouse and rabbit embryos were treated with H-Y antibody obtained from rat, 46.4 and 54.8% of embryos were retarded or destroyed. From these results it could be said that H-Y antibody had strong interspecific cross reactivity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.4
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• pp.303-310
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• 1998

We studied expression patterns of thermotolerance gene (BcHSP17.6) in cabbages which was isolated from Chinese cabbage and we will attempt transformation of forage crops with the gene in order to increase thermotolerance of forage crops. Antiserum against a BcHSP17.6 protein was reacted with its antigen. With this antiserum, the accumulation of the 15- to 18-kD LMW HSPs under various heat shock (HS) conditions was quantified. The LMW HSPs began to be detectable at $35^{\circ}C$, and after 4 hours at $40^{\circ}C$ they were accumulated to a maximum level of 1.56 micrograms per 100 micrograms of total proteins in cabbage leaves and remained almost unchanged up to 24 hours after HS. Accumulation of the HSPs was reduced at temperatures higher than $40^{\circ}C$. We conclude that accumulation of these LMW HSPs are necessary for Chinese cabbages to survive at an otherwise lethal temperature.

• The Journal of the Korean Society for Microbiology
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• v.11 no.1
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• pp.13-18
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• 1976

The rabbit anti-rat brain associated ${\theta}(RBA{\theta})$ serum wich was obtained by immunization of rabbit with DA rat brain tested against rat lymphoid tissues for cytotoxicity, indirect immunofluorescent staining and ability to inhibit a graft-vs-host reaction. It was founded that the antiserum was a potent anti-${\theta}$ like antiserum, and rat brain associated ${\theta}$ antigen was cross-reactive with mouse thymocytes and brain antigen. Using the RBA ${\theta}$ sera, distribution of ${\theta}$-bearing lymphocytes in rat lymphoid tissues was detected. And it was found that approximately 98% of thymocytes, 70-76% of lymph node lymphocytes, 72% of peripheral blood lymphocytes, 36-44% of spleen lymphocytes, and 4% of bone marrow were ${\theta}$-bearing.