• 제목/요약/키워드: antioxidative activity.

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지방질의 산화에 대한 된장 및 그 추출물의 항산화 특성 (Antioxidative Characteristics of Fermented Soybean Paste and Its Extracts on the Lipid Oxidation)

  • 최홍식;박경숙;문갑순;박건영
    • 한국식품영양과학회지
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    • 제19권2호
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    • pp.163-167
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    • 1990
  • Antioxidative effects of soybean paste(SP) on the lipid oxidation were studied with the model systems of ground cooked meat(GCM)-SP or ground cooked fish(GCF)-SF and model systems of linoleic acid mixture(LA)-SP powder (SPP) or LA-SP extract fractions during oxida-tion reaction. SP played a role as an antioxidative substance in the system employed especially in GCM-SP and the antioxidative activity was increased with the increase of SP addition in the system of GCF-SP during storage at 6$^{\circ}C$ SPP also exhibited some antioxidative activity during the oxidation reaction of LA. the activity was increased as the concentrations of SPP increased in the range from 0.1% to 0.5% Considerable antioxidative activities have been observed in both water soluble and lipid soluble fractions from SP on LA reaction system.

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자외선이 조사된 사람피부 섬유아세포에서 흰털오가피 잎추출물의 항산화작용 (Antioxidative Activity of Extracts of Acanthopanax divaricatus var. albeofructus Leaves in Human Dermal Fibroblast Irradiated by UVA)

  • 신애향;유수연;노빛나;김자인;김옥경;박원봉
    • 약학회지
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    • 제51권4호
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    • pp.229-234
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    • 2007
  • We investigated antioxidative activity of the water and ethanol extracts of leaves of Acanthopanax divaricatus var. albeofructus in human dermal fibroblast (HDFs) irradiated by UVA. The irradiation of UVA did not affect the cell viability of HDFs. The antioxidative activity of the extract was investigated by xylenol orange, TBARS (thiobarbituric acid reactive substances) and antioxidant enzyme assay. Both extracts showed H202 scavenging activity and inhibited lipid peroxidation in HDF cells irradiated by UVA. The extracts also recovered enzyme activity in the same cells.

속단의 유리기소거활성 및 저밀도지방단백질 산화에 미치는 영향 (Effects of Dipsacus asper on Free Radical Scavenging and Lowdensity Lipoprotein Oxidation)

  • 박혜선;양기숙
    • 약학회지
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    • 제50권1호
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    • pp.47-51
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    • 2006
  • The roots of Dipsacus asper are used in traditional Chinese medicine as an analgesic, enhancement of liver activity, an anti-inflammatory agent and the treatment of fractures. In order to investigate the antioxidative activity, Dipsaci Radix was extracted with MeOH and fractionated with $CHCl_3$, EtOAc, BuOH and water. MeOH ex. and its solvent fractions were determined on the free radical scavenging activity by DPPH method and antioxidative activity on low density lipoprotein oxidation. The EtOAc and BuOH fractions showed antioxidative activities and from their fractions, two compounds were isolated and identified as hederagenin 3-O-${alpha}$-L-arabino pyranoside (compound 1) and O-${\alpha}$-L-arabinopyranosyl hed-eragenin 28-O-${\beta}$-D-glucopyranosyl ($1{\to}6$)-${\beta}$-D-glucopyranosyl ester (compound 2). Saponin glycoside, compound I showed anti-oxidative activity.

목초액의 향균 및 DPPH 라디칼 소거 활성에 관한 연구 (Study of Antimicrobial and DPPH Radical Scavenger Activity of Wood Vinegar)

  • 이경민;정귀택;박돈희
    • KSBB Journal
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    • 제19권5호
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    • pp.381-384
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    • 2004
  • The antimicrobial and antioxidative activities were investigated to confirm the utility of wood vinegar. Antimicrobial activity was performed by paper disc method and liquid culture. The growth inhibition was observed in all microbial species at a dose of as low as 25 $\mu$m of wood vinegar by paper disc method. Also, in liquid culture, S. cerevisiae and P. aeruginosa were more inhibited the growth than others in the concentration of $2\%$ (v/v). For measuring of antioxidative activity, wood vinegar was fractionated with acidic, phenolic, basic and neutral fraction; and their antioxidant activities were measured by the radical scavenging effect on DPPH radical. In four fractions, phenolic fraction showed high antioxidative activity.

맨드라미, 비트, 홍갓 색소 추출물의 항산화 및 항균 효과 (Antioxidant and Antibacterial Activity of Extracts from Brassica juncea czerniak et coss., Celosia cristata L., and Beta vulgaris L.)

  • 김미혜
    • 한국식생활문화학회지
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    • 제27권6호
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    • pp.719-729
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    • 2012
  • We sought to study the qualities and scientific benefits of Dongchimi, a traditional Korean food. We compared and analyzed ingredients used for the appearance and storability of dongchimi - honggot (Brassica juncea czerniak et coss), cockscomb (Celosia cristata L.), and beet Beta vulgaris L.). We specifically examined the antioxidative and antibacterial activity of pigments from extracts of these ingredients. Distilled water ($H_2O$) and 1% citric acid were used to safely extract pigments. The antioxidative activity of the pigments was then measured for total phenolic compounds, SOD (Super Oxide Dismutase), and EDA (Electron Donation Ability) by DPPH. The antibacterial activity of was also assessed by a Paper disc solution. Our results show that the pigments had sufficient antioxidative activity and had antibacterial properties against Gram positive and negative bacteria. In particular, Cockscomb (used for enhancing color) contained the highest amount of polyphenol compounds and had the most efficient antioxidative activity.

Antioxidative and anti-inflammatory activities of Phaseolus aureus

  • Hong, In-Taik;Kim, Beom-Jun;Yu, Duck-Cho;Kim, Jung-Haeng;Kim, Jung-Han;Heo, Moon-Young;Lee, Song-Jin;Kim, Hyun-Pyo
    • 대한화장품학회지
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    • 제22권2호
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    • pp.41-51
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    • 1996
  • Phaseolus aureus (mung bean), Leguminosae, has been used as an antidote from the ancient time. Especially, it has been widely used for cleaning face and skin in oriental countries. Although several constituents such as fatty acids, phytoallexin and phaseol derivatives were reported in P. aureus and related species including seedlings, there has been a few report to describe its biological activity. Therefore, in this investigation, the ethanol extract from P. aureus was obtained and its biological activities including the antioxidative and anti-inflammatory activities were studied. The 70% ethanol extract from P. aureus showed dose-dependent antioxidative activity (52.3% inhibition at 4 mg/ml) against lipid peroxidation assay, while the extract did not show the inhibitory activity of superoxide radical formation. The extract also showed the topical anti-inflammatory activity against croton-oil and arachidonic acid induced mouse ear edema test (18-19% inhibition at 7.5 mg/ear) as well as mild inhibitory activity against picryl chloride induced delayed hypersensitivity in mouse. For investigating active principles, vitexin and isovitexin (apigenin C-glycoside) as flavonoids, and adenosine were isolated from the extract using silica gel chromatography. The actual contents of vitexin and isovitexin were found to be 3.7 and 2.4 mg/g extract, respectively. Vitexin and isovitexin showed the antioxidative activity. They showed the topical anti-inflammatory activity, although the activities were not potent compared to the reference compounds. These results suggested that vitexin and isovitexin may be, at least in part, the compounds contributing the antioxidative activity in vitro and the topical anti-inflammatory activity of P. aureus in vivo. All results of present study might be one of the scientific rationale in using mung bean for skin care from the ancient time.

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국내산 생약추출물의 항산화 효과 및 생리활성 (Antioxidative Activity and Physiological Activity of Some Korean Medicinal Plants)

  • 김현구;김영언;도정룡;이영철;이부용
    • 한국식품과학회지
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    • 제27권1호
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    • pp.80-85
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    • 1995
  • 국내산 재배 생약류의 용매추출물의 항산화력 비교를 위해 28가지 생약류를 methylene chloride로 각각 추출한 후 linoleic acid에 1,000 ppm 첨가하여 항산화력을 비교하였다. 28종의 생약 methylene chloride 추출물들은 대조구에 비해 다소 차이는 있으나 모두 항산화력을 나타내었다. 황금추출물은 BHA와 비슷한 수준의 항산화력을 보였으며, 목단과 맥문동은 황금보다는 효과가 떨어졌으나 비교적 높은 항산화 효과를 나타내었다. Methylene chloride 추출물의 항산화 효과는 황금이 가장 뛰어난 것으로 나타났으므로 극성이 서로 다른 9가지용매로 추출하였을 때 전반적으로 극성이 높아질수록 수율이 높아졌다. 국내산 생약류의 생리활성을 알아보기 위하여 열수추출물의 아질산염 소거작용, 전자공여 작용 그리고 항보체 활성을 조사하였다.

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국내산 약용식물 추출물의 항산화 효과 검색과 용매 분획물의 비교 (Antioxidative Activity of Ethanol Extract from Korean Medicinal Plants)

  • 임대관;최웅;신동화
    • 한국식품과학회지
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    • 제28권1호
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    • pp.83-89
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    • 1996
  • 116종의 생약을 75% 에탄올로 추출한 후 팜유와 돈지를 기질로 하여 Rancimat으로 항산화력을 측정하였다. 대부분의 추출물들이 항산화력을 나타내었는데 그중에서도 건강, 강황, 방기, 백작약, 석곡, 소목, 편축, 현호색, 황금 등의 에탄올 추출물이 다른 생약 추출물보다 높은 항산화력을 나타내었다. 에탄올 추출물들의 팜유에 대한 항산화력은 소목>강황>건강 순이었고, 돈지에 대해서는 소목>백작약>석곡>건강 순이었으며, 소목 추출물 1,000 ppm 첨가구의 팜유와 돈지에 대한 AI는 각각 2.16, 7.85 였다. 항산화력이 비교적 높았던 생약류의 에탄올 추출물들은 순차분획을 함에 따라서 항산화 효과가 증가되었는데, 팜유에 대한 생약류의 chloroform 및 ethyl acetate 분획물들의 항산화 활성은 소목>백작약>건강>산수유>석곡 순이었고, 돈지에 대해서는 소목>백작약>석곡>산수유>건강>강황 순이었다. 이들 추출물 중에서 소목 75% 에탄올 ethyl acetate 분획물 600 ppm 첨가구는 팜유의 유도기간을 2배 이상 증가시켰고, 돈지의 유도기간도 7배 이상 연장시켜 가장 높은 항산화 효과를 보였는데, 원인물질에 대한 연구는 계속 수행중에 있다.

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Rooibos Tea(Aspalathus linearis)에탄올 추출물의 항산화 효과 및 식품에 대한 응용 (Antioxidative Effects of Ethanol Extract Obtained from Rooibos Tea(Aspalathus linearis) and It's Application of Food)

  • 하해춘;김희숙;류병호
    • 한국식품영양학회지
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    • 제13권1호
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    • pp.13-20
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    • 2000
  • This study was designed the antioxidative effect of the ethanol extract obtained Rooibos tea (Aspalathus linearis) on various kinds of oil, and examined the synergistic effect of Rooibos tea extract by addition of citric acid to the antioxidation activity and also investigate to antioxidation effect of the extract in food production and storage peroids. The antioxidative activity of 0.2mg of Rooibos tea extract was showed similar to same doses of $\alpha$-tocopherol, BHA and BHT in linolieic acid-ethanol system. The antioxidative effect of the mixture with 0.1% Rooibos tea extract on lard was more effective than that of the mixture with same doses of $\alpha$-tocopherol, BHA and BHT. The antioxidative effect of Rooibos tea extract was showed slightly effects on lard or soybean oil. Antioxidative effects of Rooiboe tea extract in addition of citric acid as synergist showed more effective in linoleic acid-ethanol system, but did not showed in the other oils. In the application of Rooibos tea extract to food prodctuction and storage period, the antioxidative effect was more effective in biscult, preparation added lard mixed with 0.1% Rooibos tea extract.

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택사 추출물의 항균 및 항산화 효과 (Antimicrobial and Antioxidative Effects of Alismatis Rhizoma Extract)

  • 서권일;조영숙;박정로;이성태;박채규
    • 생명과학회지
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    • 제10권5호
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    • pp.524-528
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    • 2000
  • 택사(Alismatis Rhizoma)는 중국에서 많이 생산되고, 우리나라에서도 그 생산량의 50%이상이 전남 순천지방에서 생산되고 있는데, 옛부터 생약으로 많이 사용되어 왔으며, 최근 택사분말은 무좀에 도포약으로 쓰이고 있어 택사에는 다양한 생리활성이 있는 것으로 생각된다. 따라서 본 연구에서는 추출용매에 따른 택사 추출물에 대한 항균 및 항산화성에 대하여 조사하였다. Paper disc법에 의한 택사 추출물의 항균 test결과 물추출물에서는 거의 모든 시험균주에서 항균활성이 나타났으나, 메탄올 및 에탄올 추출에서는 그 활성이 작게 나타났으며, 시험균주중 Vibrio parahaemolyticus균에 가장 강한 항균활성을 나타내었다. Linoleic acid에 대한 항산화력의 조사에서 택사 물추출물 100$\mu{l}$첨가시 메탄올 및 에탄올 추출물은 항균효과 나타나지 않았고, 물추출물은 효과가 나타났으나 0.1% BHT첨가시 보다는 그 효과가 적었다.

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