• 제목/요약/키워드: antioxidant compounds

검색결과 1,804건 처리시간 0.023초

조리법에 따른 국내산 마늘종과 마늘의 항산화 효능 (Antioxidant Activities of Domestic Garlic (Allium sativum L.) Stems and Garlic Bulbs according to Cooking Methods)

  • 정지영;김창순
    • 한국식품영양과학회지
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    • 제38권2호
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    • pp.188-194
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    • 2009
  • 마늘종의 조리법으로 빈번이 사용되고 있는 데치기, 볶음처리, 간장 절임 마늘종장아찌, 고추장 절임 마늘종장아찌를 선택하여 마늘종을 조리한 후에 항산화관련 물질의 함량과 항산화 효능을 생마늘종과 비교 측정하여 마늘종을 활용한 응용식품 개발에 기초자료를 제공하고자 하였다. 생마늘종은 생마늘에 비해 알리신이나 페놀화합물 함량이 낮게 함유되었으나 클로로필, $\beta$-카로틴, 비타민 C는 현저히 높은 것으로 나타났다. 조리 후 마늘종에 함유된 클로로필과 $\beta$-카로틴 함량은 증가하였으나 비타민 C, 페놀화합물, 알리신 함량은 감소하였다. 측정된 항산화 효능은 생마늘이 EDA와 NSA가 생마늘종에 비해 현저히 높았으나 SOD는 유의적으로 낮았다. 조리 후 마늘종의 항산화 효능은 감소하여 생마늘종, 볶음 처리, 데치기, 간장 절임 마늘종장아찌, 고추장절임 마늘종장아찌 순으로 낮아졌다. 조리 후에도 마늘종의 EDA와 NSA는 마늘보다 낮게 나타났으나, 생것에 비해 그 차이가 적어져 EDA는 마늘의 약 70%, NSA는 약 65% 정도였으며, SOD 유사활성은 마늘과 유사하였다. 조리 전, 후에 나타난 항산화 효능은 한지형이 난지형보다 높았다. 따라서 항산화 효능 면에서 마늘종을 생으로 먹는 것이 가장 좋고, 기호에 따라 단시간 볶거나 데친 후에도 상당한 항산화 효능을 가지는 것으로 나타나 이를 더욱 다양하게 이용할 수 있는 방안들도 추후 연구되어져야 할 것이다.

붉나무의 부위 별 항산화 및 항염증 활성 (Antioxidant and anti-inflammatory activity of parts of Rhus javanica L.)

  • 최지수;한상돈;장태원;이승현;박재호
    • Journal of Applied Biological Chemistry
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    • 제62권2호
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    • pp.195-202
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    • 2019
  • 붉나무(Rhus javanica L.)는 동아시아에서 주로 분포하는 옻나무과의 식물이다. 붉나무의 잎, 줄기, 뿌리 에틸아세테이트 분획물의 항산화 활성 및 항염증 활성을 확인하였다. 붉나무 뿌리 에틸아세테이트 분획물의 DPPH 및 ABTS radical 소거활성이 가장 효과적이었으며, 총 페놀 화합물의 함량은 62.9, 70.3, 73.9 mg/g으로 나타났다. HPLC/PDA 분석을 통해 gallic acid를 동정 및 정량하였다. LPS로 자극시킨 RAW264.7 세포에서 항염증 활성을 확인하였다. iNOS의 발현 억제를 통한 NO 생성을 억제하였으며, 이러한 결과들을 통해 gallic acid를 포함하고 있는 붉나무는 염증 질환의 효과적인 경감 및 치료제로 개발될 수 있는 가능성을 보였으며, 붉나무 뿌리의 에틸아세테이트 분획물의 활성이 잎과 줄기 에틸아세테이트 분획물의 활성보다 뛰어났다.

수확시기별, 부위별 선학초의 폴리페놀함량 및 DPPH 라디컬 소거능의 변화 (Changes in Total Polyphenol Contents and DPPH Radical Scavenging Activity of Agrimonia pilosa According to Harvest Time and Various Part)

  • 장상훈;유은애;한기수;신성철;김희규;이상경
    • 한국약용작물학회지
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    • 제16권6호
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    • pp.397-401
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    • 2008
  • Changes in the contents of total phenolic compounds in as Agrimonia pilosa well as their antioxidant capacity according to the havest time and positions were examined. The contents of the total phenolic compounds were determined by extraction with MeOH. Among havest times harvestry in July showed highest contents of the total phenolic compounds and harvestry in May showed lowest contents of the phenolic compounds. Among the 4 positions (root, branch, leaf, flower) of Agrimonia pilosa the root contained highest contents of the phenolic compounds. The antioxidant capacities of Agrimonia pilosa were increased roughly with increasing level of contents of phenolic compounds according to positions.

Constituents and their DPPH Scavenging Activities from the Leaves of Alnus hirsuta (Spach) Rupr.

  • Dai, Yinghui;Thuong, Phuong Thien;Hung, Tran Manh;Jin, Wenyi;Cui, Zheng;Bae, Ki-Hwan
    • 한국약용작물학회지
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    • 제13권2호
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    • pp.85-90
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    • 2005
  • Phytochemical study on the EtOAc fraction from a MeOH extract of the leaves of Alnus hirsuta Rupr. led to the isolation of nine compounds betulin (1), betulinic acid (2), hirsutanonol (3), hirsutenone (4), quercetin (5), avicularin (6), gallic acid (7), hyperin (8), and daucosterol (9). Among them, six compounds 1, 2, 57, and 9 are report from this plant for the first time. All isolated compounds were evaluated for their antioxidant activity using DPPH radical scavenging capacity and inhibition effect on mitochondrial lipid peroxidation. Six phenolic compounds 3-8 were found to have potent antioxidant activity. Of which, compounds 3, 4 and 5 showed significant free radical scavenging activity with the $IC_{50}$ values of $18.3\;{\pm}\;2.5,\;15.7\;{\pm}\;3.8\;and\;23.5\;{\pm}\;3.1\;{\mu}m$, respectively. In addition, the compounds 3-8 exhibited inhibition effect on the mitochondrial lipid peroxidation with the $IC_{50}$ values of $88.0\;{\pm}\;6.5,\;12.6\;{\pm}\;1.2,\;8.0 \;{\pm}\;1.1,\;58.5\;{\pm}\;4.3,\;173.6\;{\pm}\;15.2,\;and\;75.0\; {\pm}\; 6.7\;{\mu}m$, respectively.

노각나무 가지의 Phenol성 성분 (Phenolic Compounds from the Twigs of Stewartia pseudocamellia)

  • 배종진;곽종환
    • 생약학회지
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    • 제46권4호
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    • pp.303-308
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    • 2015
  • Ten phenolic compounds were isolated from the twigs of Stewartia pseudocamellia. The isolated compounds were identified as 5,7,3',5'-tetrahydroxyflavanone (1), 3,5,7,3',5'-pentahydroxyflavanone (2), quercetin (3), (+)-aromadendrin (4), (+)-ampelopsin (5), myricetin (6), (+)-catechin (7), (-)-epicatechin (8), kaempferol (9), and fraxin (10) by spectroscopic analysis. Compounds 1, 2, 4, 6, 8, and 9 were isolated from this plant for the first time. The antioxidant activities of compounds 1-10 were evaluated by the DPPH and/or ORAC (oxygen radical absorbance capacity) assay. Compounds 3, 5-9 showed significant antioxidative effects on DPPH assay. Among the active compounds, 6 exhibited higher activity compared to trolox on ORAC assay.

꾸지 뽕나무 휘발성 향기성분의 항산화활성 (Antioxidant Activities of Volatile Aroma Components from Cudrania tricuspidata (Carr.) Bureau Extracts)

  • 고건희;남상해
    • 한국식품영양과학회지
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    • 제41권11호
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    • pp.1493-1501
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    • 2012
  • 꾸지 뽕나무(C. tricuspidata)의 휘발성 향기추출물의 항산화활성을 2 가지의 시험방법을 이용하여 측정하였다. 그리고 꾸지 뽕나무의 향기추출물에서 동정된 10종의 휘발성 향기성분에 대한 항산화활성도 측정하였다. 꾸지 뽕나무의 줄기 및 뿌리 향기추출물들은 2가지 실험법에서 모두 뚜렷하게 처리농도에 비례하여 항산화활성을 나타내었다. 즉 aldehyde/carboxylic acid assay에서 $500{\mu}g/mL$의 농도로 처리하였을 때, 줄기와 뿌리의 향기추출물은 각각 $77.02{\pm}8.12%$$74.19{\pm}6.82%$의 항산화활성을 나타내었으며, lipid/malonaldehyde assay에서 $160{\mu}g/mL$의 농도로 처리하였을 때, 줄기 및 뿌리의 향기추출물은 각각 $61.43{\pm}2.11$$76.17{\pm}4.25%$의 항산화활성을 나타내었다. 꾸지 뽕나무의 향기추출물에서 동정된 향기성분들은 7종의 terpenes and terpenoides, 14종의 alkyl compounds, 11종의 nitrogen containing heterocyclic compounds, 3종의 oxygen containing heterocyclic compounds, 12종의 aromatic compounds, 9종의 lactones와 7종의 miscellaneous compounds였다. 꾸지 뽕나무의 향기추출물에서 동정된 향기성분들 중에서 eugenol, isoeugenol 및 2,4-bis(1,1-dimethylethyl)phenol은 lipid/malonaldehyde assay에서 $160{\mu}g/mL$의 농도로 처리하였을 때, 각각 $91.74{\pm}1.33$, $94.00{\pm}0.59$$91.22{\pm}4.74%$의 항산화활성을 나타내었다. 이는 동일한 방법에서 BHT와 ${\alpha}$-tocopherol의 항산화활성이 각각 $91.90{\pm}0.42%$$89.47{\pm}3.04%$인 것과 비슷한 수준이었다. 한편 동일한 처리농도에서 vanillin과 2-acetylpyrrole은 각각 $63.36{\pm}5.83$$58.62{\pm}6.83%$로서 중간 정도의 항산화활성을 나타내었다. 따라서 꾸지 뽕나무는 산화적 손상으로 인한 질병의 예방과 사람들의 건강에 도움을 줄 수 있을 것으로 생각되었다.

Selective Butyrylcholinesterase Inhibitors Using Polyphenol-polyphenol Hybrid Molecules

  • Woo, Yeun-Ji;Lee, Bo-Hyun;Yeun, Go-Heum;Kim, Hyun-Ju;Won, Moo-Ho;Kim, Sang-Hern;Lee, Bong-Ho;Park, Jeong-Ho
    • Bulletin of the Korean Chemical Society
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    • 제32권8호
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    • pp.2593-2598
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    • 2011
  • Polyphenols (PPs) are known as antioxidant compounds having benign biological activities. In this paper, a series of hybrid molecules between the free or acetyl protected polyphenol compounds were synthesized and their in vitro antioxidant activity (DPPH assay) and cholinesterase [acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE)] inhibition activities were evaluated. As expected, free phenolic hybrid compounds (6 and 8) showed better antioxidant activity than acetyl protected hybrid compounds (5 and 7) from DPPH assay. But the contrast result was obtained from BuChE inhibition assay. Acetyl protected hybrid compounds (5 and 7) showed better inhibition activity for BuChE than free phenolic hybrid compounds (6 and 8). Specifically, 10 (AcFA-AcFA) were shown as an effective inhibitor of BuChE ($IC_{50}=2.3{\pm}0.3{\mu}M$) and also had a great selectivity for BuChE over AChE (more than 170 fold). Inhibition kinetic studies with acetyl protected compounds (5, 7, 9, and 10) indicated that 5, 7 and 10 are a hyperbolic mixed-type inhibition and 10 is a competitive inhibition type. The binding affinity (Ki) value of 10 to BuChE is $2.32{\pm}0.15{\mu}M$.

Quality Characteristics and Antioxidant Potential of Seeds of Native Korean Persimmon Genotypes

  • Kim, Il-Doo;Dhungana, Sanjeev Kumar;Kim, Hye-Ryun;Shin, Dong-Hyun
    • 한국자원식물학회지
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    • 제30권6호
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    • pp.670-678
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    • 2017
  • Persimmon seeds contain considerable amounts of minerals, amino and organic acids, natural antioxidants and phenolic compounds. The objective of this study was to investigate quality characteristics and antioxidant potential of Korean persimmon seeds. The pH (4.88-4.94), color values, contents of minerals, free amino acids, organic acids, and phenolic compounds and DPPH free radical scavenging potentials of persimmon seed extracts significantly (p < 0.05) varied with the genotypes. This study showed that the seeds could be used as a source of different mineral elements (47.14-85.07 mg/kg) without any measureable amount of heavy metals such as arsenic, cadmium, lead and mercury. Similarly, considerable amounts of organic (1550.13-2413.08 mg/kg) and essential amino (50.85-54.03 mg/kg) acids and total phenolic compounds ($1227.91-1307.78{\mu}g$ gallic acid equivalent/g) were also found in the seed extracts, indicating their potential food value as a natural antioxidant. Results of the present study imply that prethanol-A, a food preservative, can be used as an effective extraction to obtain the minerals, organic and free amino acids, and phenolic compounds from the persimmon seeds, which possess a big potential to be commercially used in food, cosmetic and pharmaceutical industries.

Antioxidant Activity of Lignan Compounds Extracted from Roasted Sesame Oil on the Oxidation of Sunflower Oil

  • Lee, Jin-Young;Kim, Moon-Jung;Choe, Eun-Ok
    • Food Science and Biotechnology
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    • 제16권6호
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    • pp.981-987
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    • 2007
  • Effects of lignan compounds (sesamol, sesamin, and sesamolin) extracted from roasted sesame oil on the autoxidation at $60^{\circ}C$ for 7 days and thermal oxidation at $180^{\circ}C$ for 10 hr of sunflower oil were studied by determining conjugated dienoic acid (CDA) contents, p-anisidine values (PAV), and fatty acid composition. Contents of lignan compounds during the oxidations were also monitored. ${\alpha}$-Tocopherol was used as a reference antioxidant. Addition of lignan compounds decreased CDA contents and PAY of the oils during oxidation at $60^{\circ}C$ or heating at $180^{\circ}C$, which indicated that sesame oil lignans lowered the autoxidation and thermal oxidation of sunflower oil. Sesamol was the most effective in decreasing CDA formation and hydroperoxide decomposition in the auto- and thermo-oxidation of oil, and its antioxidant activity was significantly higher than that of ${\alpha}$-tocopherol. Sesamol, sesamin, and sesamolin added to sunflower oil were degraded during the oxidations of oils, with the fastest degradation of sesamol. Degradation of sesamin and sesamolin during the oxidations of the oil were lower than that of ${\alpha}$-tocopherol. The results strongly indicate that the oxidative stability of sunflower oil can be improved by the addition of sesamol, sesamin, or sesamolin extracted from roasted sesame oil.

Rapid Identification of Radical Scavenging Compounds in Blueberry Extract by HPLC Coupled to an On-line ABTS Based Assay and HPLC-ESI/MS

  • Kim, Chul-Young;Lee, Hee-Ju;Lee, Eun-Ha;Jung, Sang-Hoon;Lee, Dong-Un;Kang, Suk-Woo;Hong, Sae-Jin;Um, Byung-Hun
    • Food Science and Biotechnology
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    • 제17권4호
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    • pp.846-849
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    • 2008
  • This study employed high performance liquid chromatography (HPLC) coupled to an on-line $ABTS^+$ radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled $ABTS^+$-RSD system. The negative peaks of the $ABTS^+$-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-$ABTS^+$-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.