• Journal of the Korean Magnetic Resonance Society
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• v.6 no.1
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• pp.69-77
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• 2002

During the screening of material which has the antimicrobial activity against aminoglycoside-resistant bacteria, A new material $\varepsilon$-(L-$\beta$-Iysine) polypeptide from a culture medium of Streptomyces sp.(DWGS2) was isolated, and the structure and the physicochemical properties of the new material were elucidated. The new material was separated by column chromatography of the culture medium using Dowex1$\times$2, Silica gel, and Sephadex LH20 etc. The chemical structure and molecular weight were determined with the data of various NMR experiments, MALDI mass, and ESI mass experiments. The antimicrobial activity of $\varepsilon$-(L-$\beta$-Iysine) polypeptide is not only better than equal to the activity of known aminoglycoside type of antibiotics(MIC=3.125 - 6.25ug/mL) but also effective against aminoglycoside-resistant bacteria and fungi. If the mechanism of antimicrobial activity against aminoglycoside- resistant bacteria is figured out, the $\varepsilon$-(L-$\beta$-Iysine) polypeptide can be utilized for the treatment of diseases caused by aminoglycoside-resistant bacteria.

• Korean Journal of Human Ecology
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• v.19 no.4
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• pp.709-717
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• 2010

This study investigated the properties and functions of wool and silk dyed with Catechu by examining the effects of dying conditions such as dye concentration, dying temperature, dyeing time, pH level and pre-mordants. These conditions were examined in relation to dye uptake and color changes, washing fastness, light fastness, ultraviolet-cut ability and antimicrobial ability of the dyed fabrics. Catechu showed good affinity to silk fiber. Langmuir adsorption isotherm was obtained, and so it was considered that ionic bondings are formed between Catechu and protein fiber. As the dyeing time and temperature is increased, the dyeability of both silk and wool fabrics also increases. At high temperatures the color of dyed fabrics changes from Y and YR to R. Wool is effective in using Al, Cu, Fe mordant, while silk is effective only in using only Cu mordants. The dyeability was shown to be improved at low pH levels. Additionally, both washing fastness and light fastness were shown to be low. However, the fabric color gradually changed to red was due to mailard reaction of catechol tannin causingby repeated washing and sunlight. The ultraviolet-cut ability was improved for cotton fabric dyed with Catechu. Also, dyed fabric with Catechu showed very good antimicrobial abilities at 99.9%.

• Fashion & Textile Research Journal
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• v.14 no.3
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• pp.478-485
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• 2012

The dying properties of fabrics with Chaenomelis Fructus extract were studied through an investigation of the characteristic of Chaenomelis Fructus colorants, the effect of dyeing conditions (dye temperature, dyeing concentration, and times on dye uptakes), effect of mordant, effect of UV irradiation, and color change in addition, antimicrobial ability and deodorant ability were estimated. In the UV-Visible spectrum, the wavelength of maximum absorption of Chaenomelis Fructus extract was 280 nm and showed that tannin is the major pigment component. From the increase of absorbance by UV irradiation, it was assumed that catechol tannin color was developed through UV irradiation. An increased dyeing concentration resulted in a larger dye uptake. Dye uptake increased as the dyeing time and temperature increased. Chaenomelis Fructus extract showed relatively good affinity to silk than cotton. Mordant, Fe and Cu were effective to increase the dye uptake of cotton fabric in addition, the dye uptake of silk fabric mordanted with Fe and K improved. UV irradiation let the color of dyed fabrics develop regardless of the UV irradiation stage however, UV irradiation on the dyed fabric was more effective than on the extract for the color development. Dyed silk fabric showed very good antimicrobial abilities of 99.9% in addition, deodorant ability improved in the fabric dyed with Chaenomelis Fructus extract.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.759-768
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• 1994

E coli strains isolated from pigs were investigated with respect to antimicrobial resistances and prevalence of R-plasmids. Also determined were properties of R-plasmids by plasmid conjugation, curing and southern hybridization using gene probes. All of 400 E coli strains were resistant to CL and SU, and 0.3% to 96.8% of the strains were resistant to most antimicrobials such as TC, PG, AM, SM, CP, GM, EM, NM, etc, while all strains were sensitive to AK. All strains were also multiply resistant to three to twelve antimicrobials. The resistances to PG, SM, TC, AM, CP, SU and ST were transferable and supposed to be mediated by R-plasmids which were opportunistic for transposition into chromosome. Plasmids bigger in size than chromosomal DNA were considered as R-plasmids and most plasmids in small size (<4Kb) proved as cryptic plasmids or nonconjugative R-plasmids. In a strain(No 99), AM resistant property was determined from both chromosomal DNA and R-plasmid DNA which is bigger in size than chromosome.

• Journal of Pharmaceutical Investigation
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• v.17 no.1
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• pp.1-14
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• 1987

To increase the bioavailability of norfloxacin, inclusion complex of antimicrobial agent norfloxacin with ${\beta}-Cyclodextrin$ was prepared and studied by the solubility method, spectrophotometric methods(UV, IR, $^1H-NMR$), differential thermal analysis, powder X-ray diffractometry, the physical properties, the antimicrobial activity, DNA binding and in situ recirculation technique. The conclusions are summerized as following; 1) The inclusion complexation was identified by means of solubility, spectrophotometry(UV, IR, NMR), DTA and X-ray diffraction. 2) The molar ratio of $norfloxacin-{\beta}-cyclodextrin$ complex was 1 : 1. 3) The stability constant of $norfloxacin-{\beta}-cyclodextrin$ complex was $21.5\;M^{-1}$, and both true and apparent partition coefficients of the inclusion complex were larger than those of norfloxacin. 4) The time required to dissolve 60% $(T_{60}%)$ of the inclusion complex was 120 min. in distilled water and in the artificial intestinal juice, while norfloxacin did not reach to 60% dissolution within 120 min. 5) The antimicrobial activity of the inclusion complex against Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus showed no significant difference compared to that of norfloxacin alone. 6) Studies on binding properties between the inclusion complex and norfloxacin alone to DNA according to equilibrium dialysis showed no significant differency. 7) In situ absorption rates (Ka) of inclusion complex and norfloxacin alone were 0.229 and $0.102hr^{-1}$, respectively.

• Journal of Fashion Business
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• v.20 no.5
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• pp.76-86
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• 2016

In this research, the dyeing properies of Amur cork tree on sheep leathers were evaluated. The proper dyeing conditions were identified with K/S values depending on the colorant concentration, dyeing time, dyeing temperature and dye bath pH. For the proper conditions, the changes of color for different mordants(Al, Cu and Fe) were observed as $L^*a^*b^*$ coordinates and H V/C values. The color fastnesses were also examined, and the antimicrobial properties were examined. The results were as follows: For the sheep leather, the optimized dyeing conditions were 300% o.w.f., 40 min., $40^{\circ}C$, and pH 5. The leather color was yellow in the dyeing and mordanting. Although the $L^*a^*b^*$ changed with the mordants and mordanting methods, the overall hue was yellow. The colorfastness to light was reduced compared to original(untreated) sheep leather. The rubbing fastness was generallry satisfactory as 4-5 rating. The dry-cleaning fastness(stain) of leathers were generally at the 4-5 level, but dry-cleaning fastness(fade) was a little reduced compared to original sheep leather. The dyed and pre-mordanted and dyed sheep leather showed excellent antimicrobial properties.

• Journal of the korean veterinary medical association
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• v.20 no.2
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• pp.103-108
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• 1984

A total of 359 cultures of Staphylococcus were isolated from no raw milk samples in stock farms of Gyeongnam province from December, 1981 to May, 1982. Some biochemical properties and antimicrobial drug susceptibility of the isolaes were studied with the

• International Journal of Oral Biology
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• v.38 no.4
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• pp.141-147
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• 2013

It has been established that berberine has strong antimicrobial effects. Little is known however regarding the antimicrobial activity of berberine against endodontic pathogenic bacteria or its cytotoxicity in human oral tissue cells. The antibacterial properties of berberine were tested against 5 strains of Enterococcus faecalis and type strains of Aggregatibacter actinomycetemcomitans, Prevotella nigrescens, Prevotella intermedia, and Tannerella forsythia, which are involved in endodontic infections. Antimicrobial activity was evaluated through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) measurements. The viability of normal human gingival fibroblast (NHGF) cells after exposure to berberine was measured using a methyl thiazolyl tetrazolium (MTT) assay. The data showed that berberine has antimicrobial effects against A. actinomycetemcomitans with an MIC and MBC of $12.5{\mu}g/ml$ and $25{\mu}g/ml$, respectively. In the cytotoxicity studies, cell viability was maintained at 66.1% following exposure to $31.3{\mu}g/ml$ berberine. Overall, these findings suggest that berberine has antimicrobial activity against the tested bacteria. Nevertheless, lower concentrations in combination with other reagents will need to be tested before these in vitro results can be translated to clinical use.

• Textile Coloration and Finishing
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• v.29 no.4
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• pp.247-255
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• 2017

Scutellaria Baicalensis(SB) is widely used in traditional and modern oriental medicine. It possesses several biology activities such as anti-oxidative, anti-inflammatory, antimicrobial and antiviral activities. In this study, a functional high density polyethylene (HDPE) fabric with antimicrobial properties was developed using zeolite microparticles as a SB extract delivery carrier. Zeolites loaded with SB extract were prepared by immersing in an SB extract aqueous solution. The average size of the SB extract-loaded zeolites was about 0.1 to $2.0{\mu}m$, and the morphology of the zeolites was not altered after SB extract binding. The resulting SB extract-loaded zeolites were then immobilized homogeneously onto the HDPE fabric using acrylic binder. The encapsulation efficiency of SB extract to the zeolite was more than 45%. The in vitro release test of SB extract-loaded zeolites containing HDPE fabrics showed release of 35% of the total SB extract by day 1 in a 24hours immersion study. Moreover, the SB extract-loaded zeolites containing HDPE fabrics showed effective antimicrobial activity against Streptococcus mutans, Staphylococcus aureus, and Klebsiella pneumoniae, indicating that this innovative delivery platform potently imparted antimicrobial activity to the HDPE fabric. In conclusion, the current study suggests that the HDPE fabric containing the SB extract-loaded zeolites microparticle carrier system has potential as an effective antimicrobial textile such as safety gloves, protective gloves etc.

• The Journal of Internal Korean Medicine
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• v.31 no.2
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• pp.290-297
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• 2010

Objectives : This study evaluated the effect of Dioscoreae Rhizoma on antioxidant and antimicrobial activity in vitro. Methods : In vitro experiments were made on antioxidant by DPPH radical scavenging activity, enzyme activity of SOD, protective effect about $H_2O_2$ and DNA fragmentation in RAW264.7 cells, as well as antimicrobial by Cox-2 expression in LPS-induced RAW264.7 cells. Results : 1. Dioscoreae Rhizoma's antioxidant ability was improved by DPPH radical scavenging activity. 2. Antioxidant ability of Dioscoreae Rhizoma produced good results at more than 0.025 mg/$m{\ell}$. 3. On protective effect of Dioscoreae Rhizoma about $H_2O_2$ on RAW264.7 cells, with addition of Dioscoreae Rhizoma extract the cells' survival increased compared to adding $H_2O_2$ only. 4. About revelation-control of Cox-2 at RAW264.7 cells which induce by LPS, when Dioscoreae Rhizoma's concentration was higher from 0.0125 mg/$m{\ell}$ to 1 mg/$m{\ell}$, its control activity was stronger. Thus we found Dioscoreae Rhizoma has antimicrobial activity. 5. The power of DNA fragmentation control of Dioscoreae Rhizoma was time-independent. Conclusions : From this study we conclude that Dioscoreae Rhizoma has effects on antioxidant and antimicrobial activity. However, considerable work still needs to be done studying antioxidants by concentration, as well as a variety of experiments about antimicrobial activity.