• Tuberculosis and Respiratory Diseases
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• 제40권5호
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• pp.483-494
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• 1993

석탄산으로 사멸한 균으로 감작시켜 얻은 토끼 항혈청으로 주요 병원성 mycobacteria의 세포 표면항원간의 항원적 상관관계를 효소결합면역분석법으로 관찰하였다. M, avium-intracellulare(MAI) 항혈청을 이용한 동종 및 이종반응 분석결과를 보면 동종내 항원적 상관관계는 매우 가깝고 M. scrofulaceum(MG)을 포함하면 MAIS군내 종간 항원적 관계도 가까웠다. MAI 혈청이 M. chelonei(MC), fortuitum(MF), nonchromogenicum(MNC), terrae(MTR) 및 triviale(MTV)와는 반응하지 않거나 미약하게 반응하지만 M. kansasii(MK)와 결핵균과는 강하게 반응했다. 그러나 후자의 두균종으로 흡수해도 MAI 혈청의 동종반응은 감소되지 않았다. M. avium(MA) (K40004) 항혈청은 M. intracellulare(MI) 균주를 제외한 다른 균들과는 미약하게 반응했고 항원적 상관관계가 가까운 MI와 MG로 흡수하면 동종반응의 50~89%가 감소했다. MI 혈청의 종내반응이 종간반응보다 물론 더 강하지만 공유항원량의 크기에 따라 달랐다. MI균주 중 N-260D, N-260R, N-260T 및 K41014간의 항원적 상관관계가 N-242D, N257T, N281D 및 N275T와의 관계보다 더 가깝게 나타났다. MNC 항혈청은 MC와 MTV를 제외하고는 강한 교차반응을 보여주었다. 종내 항원적 상관관계 다음으로는 MTR와 MG(K30003)과 가까웠다. NC-3 혈청은 MA, MC 및 MF와 상당한 반응력을 나타내지만 NC-11은 그렇지 않았다. MTR 항혈청은 MI와 강하게 반응하지만 MI로 흡수한다고 해서 그들의 동종반응이 감소하지 않는 것으로 보아 MTR 표면에 공유항원이 많지 않음을 보여주었다. MTR과 MNC, MC 및 MF 사이에도 상당량의 공유항원이 있음이 알려졌다. MTR 가운데 T-8 혈청과는 달리 T-13 항혈청은 MA, MG, MK 및 MTB와 강하게 교차반응하였다. 이상의 실험으로 밝혀진 mycobacteria의 항원적 상관관계가 각종 생물학적 및 생화학적 방법으로 갈라놓은 분류군과 잘 일치하고 있었다.

• Journal of Plant Biology
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• 제36권1호
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• pp.91-96
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• 1993

Phycoerythrins from the ceramiaceous red algae Campylaephora crassa (Okamura) Nakamura and related species, C. hypnaeoides J. Agardh and Ceramium kondoi Yendo, were investigated for absorption spectra, protein bands by gel electrophoresis and antigenic reactivity to anti-phycoerythrin using Ouchterlony double diffusion and immunoblot. Similarities in absorption spectra, showing peaks at ca. 566 nm>534 nm>495 nm, were found between C. crassa and Cm. kondoi, while C. hypnaeoides differed slightly. There were no differences in fluorescence emission spectra and protein bands between C. crassa and related species tested. Since Ouchterlony double diffusion, however, showed that phycoerythrins from C. crassa and Cm. kondoi were similar in antigenic reactions, and differed from that of C. hypnaeoides, the taxonomic position of C. crassa should be reinvestigated using other experimental approaches.

• 한국연초학회지
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• 제6권2호
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• pp.141-146
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• 1984

Two PVY strains (PVY-VB and PVY-VN) isolated from tobacco in Korea were compared for their serological relationship with other 8 strains which were obtained from tobacco or potato in different countries. One of these strains, PVY-Argentina showed the spur reaction to PVY-VB and PVY-VN antisera in SDS-agar gel double diffusion plates. The two Korean PVY strains were closely related to other strains except for one, PVY-Argentina when antigen-antibody reciprocal absorption tests were conducted. It is suggested that the strain, PVY-Argentina, is a new serotype containing a specific antigenic site different from other 9 strains tested.

• 대한수의학회지
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• 제32권2호
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• pp.195-205
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• 1992

The soluble antigen profiles and antigenic specificities of Leptospira interrogans serovars icterhaemorrhagiae, canicola, pomona and hardjo were examined by SDS-polyacrylamide gel electrophoresis, crossed immunoeletrophoresis and immunoblotting. The profiles of protein, glycoprotein and fraction containing N-acetylglucosamine of 4 serovars were compared. The protein profiles of 4 serovars were very similar except the range of 14,400 to 30,000 daltons. Molecular weight of glycoprotein of L, pomona was lower than other serovars. L canicola showed extra N-acetylglucosamine bands having molecular weight of 82,000 and 90,000 daltons. In crossed immunoelectrophoresis, a close antigenic relationship was found between L icterohaemorrhagiae and L canicola. In immunoblottings conducted with soluble antigens and rabbit antisera of 4 serovars, Leptospira interrogans serovars possessed cross-reactive antigens and serovar-specific antigens. The molecular weights of serovar-specific antigens were 45,000, 82,000 and 90,000, 31,000 and 24,000 daltons in L icterohaemorrhagiae, L canicola, L pomona, and L hardjo, respectively.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.290-295
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• 1994

30 monoclonal antibodies (mAbs) were produced against Bacillus thuringiensis subsp. canadensis. Out of the these, 6 mAbs were selected for further studies. SDS-PAGE analyses of sonicated antigens of 10 8. thuringiensis strains showed that they generally had both predominant protein antigens of molecular weights of 45 kilodalton (kd) except for shandogiensis and konkukian, and 37kd except for israelensis, tochigiensis, and shandogiensis, respectively. These results indicate that 4kd and 37kd may be important for demonstrating common antigens except for a few strains of B. thuringiensis. In comparing the result of the westem blot using mAbs with that of using polyclonal antibodies to canadensis, we found that immunoreactive proteins of 99 and 39 kd were identified as common antigens, which might act as antigenic determinants, and might be surface or flagella antigens. Reactivities of mAbs with 41 strains of 8. thuringiensis demonstrated that mAbs of C-1, C-3, C-4, C-S and C-6 except C-2 did not recognize epitopes of thuringiensis, but that all of the mAbs recognized epitopes of galleriae, kurstaki, dakota, tolrJokuensis, silo, toguchini, and leesis. The potential applications of the mAbs we produced would be useful tools for the clarification of taxonomy, investigation of antigenic relationship between B. thuringiensis strains, and localization of specific surface and flagella antigens.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.315-315
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• 1994

Recently, it was reported that Pfaffia paniculata, known as Brazil ginseng, caused occupational asthma. To assess a potential relationship between Korean ginseng and allergic reactions, total IgE in serum, allergologic parameter, was investigated. Serum total IgE were measured in 8 takers of ginseng for more than 5 yr, 4 subjects of weak constitution, 10 workers participated in the washing of fresh ginseng in factory and 7 control subjects, Blood samples were taken before and after the scheduled exposure to Korean ginseng, sera were kept frozen until assay. Total IgE levels after the scheduled exposure to Korean ginseng were not significantly different in each group and total IgE levels were not significantly different in the control subjects compared with those of other groups at the start of experiment and at the end of experiment. These results suggest that the exposure to Korean ginseng dose not affect significantly the IgE immune response in volunteers. No changes of levels of IgE on the exposure of Korean ginseng may represent additional evidence of the lack of antigenic potential of Korean ginseng.

• 대한미생물학회지
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• 제20권1호
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• pp.109-113
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• 1985

It was well known that B. pertussis cells possess protective antigen, histamine sensitizing factor, heatstable and labile toxin, hemagglutinin, agglutinogen and the others. Previous reports involving above antigenic properties of B. pertussis have been carried out for several years. However, leucocyte promoting property was not yet investigated. In this report, the results of studies on the leucocytosis, particulary the lymphocytosis, produced in mice by injecting pertussis vaccine were presented. Especially leucocyte promoting property and histamine sensitizing property of B. pertussis vaccine treated at various temperatures were compared. The relationship between the leucocyte promoting property and histamine sensitizing property was investigated. Results were as follows. 1. Although leucocytosis was significantly rised in both 0.5ml injection and 0.1ml injection of pertussis vaccine than in control, at the higher dose (0.5ml injection) an elevation in white cell count was more significant. The leucocyte responce to pertussis vaccine was greater following 0.5 ml injection than following 0.1ml injection. 2. Lymphocytosis was significantly rised in both 0.5ml injection and 0.1ml injection of pertussis vaccine than in control. At higher dose (0.5ml injection), an elevation in lymphocyte count was more significant. 3. Order of elevation in differential leucocyte counts was lymphocyte, polymorphonuclear leucocyte and monocyte. 4. The leucocyte response to pertussis vaccine was 2 fold greater following intravenous injection than following subcutaneous injection. 5. Decrease leucocyte promoting activity and histamine sensitizing activity resulted from exposure to temperature above $56^{\circ}C$. Histamine sensitizing activity of pertussis vaccine treated at various temperatures paralleled leucocyte promoting activity.

• The Plant Pathology Journal
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• 제18권1호
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• pp.6-11
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• 2002

An isolate of Cucumber mosaic virus (CMV), PaFMl-CMV causing malformation on the fruit of paprika (Capsicum annuum var, grossum) was characterized based on biological reactions, serological relationships, and partial nucleotide sequence analyses. PaFMl-CMV was distinguishable from other isolates of CMYI Mf-(subgroup I) and LS-CMV (subgroup II), in terms of its reactions to some host plants. Polyclonal antibody against PaFMl-CMV showed homologous antigenic relationship with LS-CMV, however, the antibody formed a spur between PaFMl- and Mf-CMV, In the comparison of molecular size of dsRNAs of PaFMl-CMV with Mf- and LS-CMV, PaFMl-CMV had a slightly smaller RNAl and larger RNA2, RNA3, and RNA4. When the CDNA product of PaFMl-CMV coat protein (CP) gene was digested with some restriction enzymes, the fragment pattern was identical with that of LS-CMV The nucleotide and amino acid sequences of PaFMl-CMV CP gene were 99.5% and 98.6% identical with LS-CMV respectively. The data indicate that PaFMl-CMV belongs to subgroup II of CMV, which is the first report in Korea.

• 대한수의학회지
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• 제46권4호
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• pp.355-361
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• 2006

Porcine epidemic diarrhea virus (PEDV) strain Chinju99, which was previously isolated from piglets suffering from severe diarrhea was used to characterize the membrane (M) protein gene to establish the molecular information, and the results will be useful in elucidating concepts related to molecular pathogenesis and antigenic structures of PEDV isolates. The Chinju99 M gene generated by reverse transcription and polymerase chain reaction (RT-PCR) consisted of 681 bases containing 22.3% adenine, 22.3% cytosine, 23.1% guanine and 32.3% thymine nucleotides, and the GC content was 45.4%. It had some nucleotide mismatches from M gene of other PEDV strains, such as CV777, Br1/87, KPEDV-9, JMe2, JS2004-2 and LJB-03 with 97-99% nucleotide sequence homology to these strains. Also, it encoded a protein of 226 amino acids, which had some mismatches from those of CV777, Br1/87, KPEDV-9, JMe2, JS20004-2 and LJB-03, as the amino acid sequence homology showed a 97-98% to these strains. The Chinju99 had a very close relationship to the Japanese strain JMe2 for the nucleotide and amino acid sequences of the M gene. The amino acids predicted from Chinju99 M gene consisted of mostly hydrophobic residues and contained three potential sites for asparagine (N)-linked glycosylation, two serine (S)-linked phosphorylation sites by protein kinase C, and two S- or threonine (T)-linked phosphorylation sites by casein kinase II.

• 대한수의학회지
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• 제52권4호
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• pp.223-230
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• 2012

The worldwide distribution and continuing genetic mutation of avian influenza virus (AIV) has been posed a great threat to human and animal health. A comparison of 3 isolates of AIV H9N2, A/chicken/Korea/KBNP-0028/00 (H9N2) (KBNP-0028), A/chicken/Korea/SNU8011/08 (H9N2) (SNU 8011) and an inactivated oil vaccine strain A/chicken/Korea/01310/01 (H9N2) (01310), was performed. The former 2 AIVs were isolated from field cases before and after the application of an inactivated H9N2 vaccine in 2007, respectively. The antigenic relationship, viral shedding, tissue tropism and genetic analysis were examined. The comparison of virus shedding from the cloaca and the oropharynx revealed that both isolates were more frequently isolated from the upper respiratory tract (90~100%) 1 day post inoculation (DPI) compared with isolation 5 DPI from gastrointestinal tracts (10~60%). Moreover, the isolate KBNP-0028 were recovered from all organs including bone marrow, brain and kidneys, indicating higher ability for broad tissue dissemination than that of SNU 8011. KBNP-0028 replicated earlier than other strains and with a higher titer than SNU 8011. In full-length nucleotide sequences of the NA gene and a partial sequence of the HA gene of SNU 8011, we found that there might be significant changes in tissue tropism, virus replication and genetic mutation in AIV H9N2 isolates.