Browse > Article
http://dx.doi.org/10.5423/PPJ.2002.18.1.006

Characterization of Cucumber mosaic virus Subgroup II Isolated from Paprika (Capsicum annuum var, grossum) in Korea  

Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration)
Kim, Jae-Hyun (National Horticultural Research Institute, Rural Development Administration)
Choi, Jang-Kyung (Division of Biological Environment, College of Agriculture and Life Science, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.18, no.1, 2002 , pp. 6-11 More about this Journal
Abstract
An isolate of Cucumber mosaic virus (CMV), PaFMl-CMV causing malformation on the fruit of paprika (Capsicum annuum var, grossum) was characterized based on biological reactions, serological relationships, and partial nucleotide sequence analyses. PaFMl-CMV was distinguishable from other isolates of CMYI Mf-(subgroup I) and LS-CMV (subgroup II), in terms of its reactions to some host plants. Polyclonal antibody against PaFMl-CMV showed homologous antigenic relationship with LS-CMV, however, the antibody formed a spur between PaFMl- and Mf-CMV, In the comparison of molecular size of dsRNAs of PaFMl-CMV with Mf- and LS-CMV, PaFMl-CMV had a slightly smaller RNAl and larger RNA2, RNA3, and RNA4. When the CDNA product of PaFMl-CMV coat protein (CP) gene was digested with some restriction enzymes, the fragment pattern was identical with that of LS-CMV The nucleotide and amino acid sequences of PaFMl-CMV CP gene were 99.5% and 98.6% identical with LS-CMV respectively. The data indicate that PaFMl-CMV belongs to subgroup II of CMV, which is the first report in Korea.
Keywords
Cucumber mosaic virus; paprika subgroup;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumovirus with a genus specific single pair of primers. J. ViroI. Methods 83:67-73   DOI   ScienceOn
2 Laemmli, U. K. and Favre, M. 1973. Maturation of the head of bacteriophage T4. I. DNA packaging event. J. Mol. Biol. 80:575-599   DOI   PUBMED
3 Owen, J., Shintaku, M., Aeschleman, P., Tahar, S. B. and PaIukaitis, P. 1990. Nucleotide sequence and evolutionary relationship cucumber mosaic virus (CMV) strains: CMV RNA3. J. Gen. ViroI. 11:2243-2249
4 Quemada, H., Keaey, C., Gonsalves, D. and Slightom, J. L. 1989. Nucleotide sequence of the coat protein genes and flanking region of cucumber mosaic virus strain C and WL RNA3. J. Gen. ViroI 70:1065-1073   DOI   ScienceOn
5 Takanami, Y. 1981. A striking change in symptoms on cucumber mosaic virus-infected tobacco plants induced by a satellite RNA. Virology 109:120-126   DOI   PUBMED   ScienceOn
6 Shintaku, M. and Palukaitis, P. 1990. Genetic mapping Of cucumber mosaic virus. In Viral Genes and PIant Pathogenesis, ed. by Pirone, T. P. and Shaw, J. G., pp. 157-164, Spriger-Verlag, New York
7 Gonda, T. J. and Symons, R. H. 1978. The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: Its application to several strains of cucumoviruses. ViroIogy 88:361-370
8 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic viras. Adv. Virus Res. 41:281-348   DOI
9 Davies, C. and Symons, R. H. 1988. Further implication for the evolutionary relationship between triparticle plant viruses based on cucumber mosaic virus RNA3. ViroIogy 165:216-224
10 Choi, H. S., Ryu, J. K., Ahn, K. K., Cho, J. D. and Kim, J. S. 2001. Cucumber mosaic Cucumovirus-CARNA5 causing bud necrosis on table tomato. PIant Pathol. J. 17:169-173
11 Francki, R. I. B., Randles, J. W., Chambers, T. C. and Wilson, S. B. 1966. Some properties of purified cucumber mosaic virus (Q strain). Virology 28: 729-741   DOI   ScienceOn
12 Wahyuni, W. S., Dietzgen, R. G., Hanada, K. and Francki, R. I. B. 1992. Serological and biological variation between and within subgroup I and II strains of cucumber mosaic virus. Plant Pathol. 41:282-297   DOI
13 Morris, T. J. and Dodds, J. A. 1979. Isolation and analysis Of double- stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854-858   DOI
14 Mossop, D. W., Francki, R. I. B. and Gribvell, C. J. 1976. Comparative studies on tomato aspermy and cucumber mosaicviruses. V. Purification and properties of a cucumber mosaic virus inducing severe chlorosis. Virology 74:544-546   DOI   ScienceOn
15 Ouchterlony, O. 1968. Handbook of immunodiffusion and immunoelectrophoresis. Prog. Allergy. Ann. Arbor Sci. Publ. 135 p
16 Rizos, H., Gunn, L. V., Pares, R. D. and Gillings, M. R. 1992. Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. J. Gen. Virol. 73:2099-2103   DOI   ScienceOn
17 Nitta, N., Masuta, C., Kuwata, S. and Takanami, Y. 1988. Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J. Gen. Virol. 69:2695-2700   DOI
18 Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of cucumber mosaic virus isolates in Korea. Korean J. Plant PathoI. 14:7-12
19 Edwards, M. C. and Gonsalves, D. 1983. Grouping of seven biological defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73:1117-1120   DOI