• Korean Journal of Veterinary Research
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• v.35 no.1
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• pp.115-121
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• 1995

The herb, Artemisia(A) annua, a member of the composital family, has been used successfully for malaria(Plasmodium sp) patients in china. Since the genera of Plasmodium and Eimeria(E) tenella are related, it is presumed that A annua may also be effective against E tenella. In order to study the anticoccidial effects of A annua the chickens inoculated with E tenella were treated with the extract of A annua in drinking water and the results were compared to those of non-medicated, infected control(NIC) and non-medicated, non-infected control(NNC) group. A annua demonstrated anticoccidial effects by showing, compared to NIC group, improved results in all parameters, such as bloody diarrhea, lesion scores, the numbers of excereted oocysts in feces, body weight gain and feed conversion rate. Anticoccidial index(ACI) of the A annua treated group (123.4) was higher than that of NIC group (96.9). These results indicate that Artemisia annua has anticoccidial effects on the Eimeria tenella.

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.129-132
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• 1998

Avian coccidiosis is a very important disease of poultry industry. In recent years, many researchers studied for the immunology of avian coccidiosis. Coccidial vaccines have been used at some poultry farms in many countries. In order to evaluate the anticoccidial effects of coccidial vaccine to chicken treated with anticoccidial drugs, 4 week-old chicken were administered anticoccidial drugs and challenged with avian coccidia at 2 weeks after vaccination of coccidia. The body weight gains, lesion scores, oocyst indices and anticoccidial indices of chicken were investigated at the time of vaccination, challenge and necropsy. The body weight gains and feed conversion rates of groups immunized with coccidial vaccine and treated with anticoccidial drugs were moderately higher and lower than those of groups treated with anticoccidial drugs, respectively. The lesion score and oocyst indices of all groups immunized with coccidial vaccine and/or treated with anticoccidial drugs were milder than those of infected control groups. The anticoccidial indices of groups immunized with coccidial vaccine and treated with anticoccidial drugs were moderately higher and lower than those of groups. treated with anticoccidial drugs. But all of the investigated items were not statistically significant.

• Korean Journal of Veterinary Research
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• v.35 no.1
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• pp.123-130
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• 1995

Artemisinin, a sesquiterpene lactone, is isolated from the leafy portion of the Artemisia annua and has been known to be effective against Plasmodium species. Since the genera of Plasmodium and Eimeria(E) tenella are included the same order, Eucoccidiidae, it is presumed that artemisinin may also be effective against E tenella. In order to study the anticoccididal effects of artemisinin, the chickens inoculated with E tenella were treated with artemisinin at different concentrations as feed additive and the results were compared to those of non-medicated, infected control(NIC) and non-medicated, non-infected control(NNC) group. Artemisinin demonstrated anticoccidial effects by showing, compared to NIC group, improved results in all parameters, such as bloody diarrhea, lesion scores, the numbers of excreted oocystis in feces, body weight gain and feed conversion rate. Anticoccidial index(ACI) of artemisinin treated group (5ppm~50ppm) was higher than that of NIC group. Improvements were greatest in the group treated with artemisinin 50ppm with an ACI of 147.6. These results indicate that artemisinin has anticoccidial effects on the Eimeria tenella.

• Parasites, Hosts and Diseases
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• v.59 no.4
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• pp.403-408
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• 2021

Avian coccidiosis has a major economic impact on the poultry industry, it is caused by 7 species of Eimeria, and has been primarily controlled using chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. We assessed anticoccidial effects of berberine-based diets in broiler chickens following oral infection with 5 Eimeria species (E. acervulina, E. maxima, E. tenella, E. mitis, and E. praecox). When 0.2% berberine, a concentration that does not affect weight gain, was added to the diet, the 4 groups infected with E. acervulina, E. tenella, E. mitis, or E. praecox showed significant reductions in fecal oocyst shedding (P<0.05) compared to their respective infected and untreated controls. In chickens treated 0.5% berberine instead of 0.2% and infected with E. maxima, fecal oocyst production was significantly reduced, but body weight deceased, indicating that berberine treatment was not useful for E. maxima infection. Taken together, these results illustrate the applicability of berberine for prophylactic use to control most Eimeria infections except E. maxima. Further studies on the mechanisms underlying the differences in anticoccidial susceptibility to berberine, particularly E. maxima, are remained.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.9
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• pp.1262-1266
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• 2005

Twelve Korean native goats, spontaneously infected with mixed species of Eimeria were used to study the possible direct anticoccidial effect of feeding condensed tannin-containing plants on the production of Eimeria oocysts. The effects of feeding pine (Pinus densifora) needles, oak (Quercus acutissima) leaves and lucerne chaff on coccidia oocyst output were studied for a period of 10 days post-feeding. The results indicate that feeding fresh pine needles (40 g condensed tannins (CT) dry matter (DM)/day/goat) and oak leaves (40 g CT DM/day/goat) in combination with lucerne chaff had rapid anticoccidial activities in goats as demonstrated by a sharp decrease in oocyst production. Two days after feeding, the numbers of oocysts per gram of faeces (OPG) from the goats fed pine needles with lucerne chaff, and from goats fed oak leaves reduced by 40% and 44% compared to pre-feeding, respectively. On the sixth day after commencing feeding pine needles and oak leaves, the reduction was 81% and 72%, respectively. Ten days after feeding pine needles and oak leaves, the OPG was reduced by 93% and 85%, respectively compared to pre-feeding. Statistical analysis showed that feeding pine needles and oak leaves to goats naturally infected with coccidia significantly (p<0.001) reduced the numbers of oocysts compared to the control group fed lucerne chaff only. Four clinically important species of coccidia, Eimeria parva, Eimeria ninakohlyakimovae, Eimeria christenseni and Eimeria arloingi were identified in Korean native goats.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1592-1597
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• 2013

Type of dietary direct-fed microbials (DFMs) or poultry litter could directly influence the composition of gut microbiota. Gut microbiota plays an important role in shaping the developing immune system and maintaining the homeostasis of the mature immune system in mammal and chickens. The present study was carried out to investigate the interaction among litter, DFMs and immunity in broiler chickens exposed to a field-simulated environment. Immune status of broiler chickens was assessed by serum antibodies against Eimeria spp. and Clostridium spp. and intestinal cytokine mRNA expression. The current experimental design had a $3{\times}2$ factorial arrangement of treatments with three types of litter, i.e., fresh litter or used litter that was obtained from a farm with no disease outbreak (used litter) or a farm with history of a gangrenous dermatitis outbreak (GD litter), and two dietary treatments with or without DFMs. It was found that either DFM addition or type of litter significantly affected anticoccidial antibody levels of broiler chickens at d 42. In general, dietary DFMs increased the anticoccidial antibodies in the fresh-litter raised chickens, but lowered the levels in the GD-litter raised chickens. Serum antibodies against Clostridium perfringens ${\alpha}$-toxin were significantly (p<0.05) higher in chickens raised on GD litter compared with those raised on fresh litter. Cytokine mRNA expression was significantly (p<0.05) altered by either the type of litter or DFMs. Of interest, dietary DFMs lowered interferon-${\gamma}$, interleukin 1beta, and CXCLi2 cytokine mRNA expression in chickens raised on fresh litter but increased them in GD-litter raised chickens. In conclusion, dietary DFMs modulate various immune parameters of broiler chickens, but the DFM-mediated effects were dependent upon the type of litter on which chickens were raised.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.7
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• pp.981-989
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• 2014

The objective of the present study was to investigate the effects of a mixture of functional oils (Essential, Oligo Basics Agroind. Ltda) on performance response of chickens challenged with coccidiosis and the determination of apparent metabolizable energy (AME), nitrogen-corrected apparent metabolizable energy (AMEn), the coefficients of protein and ether extract digestibility and intestinal morphology of broilers fed with diets containing Essential. In Exp. 1, a completely randomized design (CRD) was used, with one control diet without Essential inclusion with coccidiosis (Eimeria acervulina, Eimeria maxima, and Eimeria tenella) challenged birds and two different inclusion rates of Essential (1.5 kg/ton and 2 kg/ton) with coccidiosis-challenged and non-challenged birds for each inclusion rate, using 10 replicates and 50 birds per experimental unit. After 7 d of coccidiosis challenge, the livability was approximately 10% lower (p<0.05) for the control group. Intestinal lesion scores were lower (p<0.05) in the anterior intestine and the cecum for the chickens supplemented. Feed efficiency and growth rate were improved in birds supplemented with Essential (p<0.05) before the coccidiosis challenge and during the first 7 d post infection. In Exp. 2, a CRD was used, with one control diet without Essential inclusion and one diet with inclusion of Essential (1.5 kg/ton), using nine replications and 33 chicks per pen. The diets with Essential yielded approximately 4% higher AME (p = 0.003) and $AME_n$ (p = 0.001). Essential supplementation increased villus height in the jejunum on d 14 (p<0.05). Villus height:crypt depth ratio for the supplemented birds was larger (p<0.05) in the jejunum on d 7, larger (p<0.05) in the jejunum and ileum on d 14. In conclusion, these functional oils improved the energy utilization and the livability and decreased lesions caused by coccidiosis in supplemented birds.

• Korean Journal of Veterinary Research
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• v.33 no.4
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• pp.657-664
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• 1993

To reveal the immunogenicity of ${\gamma}-irradiated$ E tenella and its progeny, a series of experiments on the effects of Cobalt-to ${\gamma}-irradiation$ was performed. The SPF chickens inoculated with diffenrt doses of inoculum were challenged with $1{\times}10^5$ oocysts of virulent E tenella. The levels of 100 Gy ${\gamma}-irradiation$ from $^{60}Co$ and of inoculum with $1{\times}10^4$ oocysts were recognized as proper as immunogen by comparison of survival rates, body weight gains, blood in feces and lesion scores in the chickens. In these trials of challenge with virulent E tenella after inoculation with $1{\times}10^4$ oocysts of the ${\gamma}-irradiated$ E tenella and its progeny, the survival rates of the chickens challenged with the virulent E tenella after immunization with the 1st and the 3rd progeny groups of ${\gamma}-irradiated$ E tenella oocysts were higher(l00%) than that(87.0%) of the challenged control group. The signs of blood in feces and the lesion scores were seen markedly lower with the ourput of the smaller number of oocysts, i.e. OPG 103,900 and 25,800 in the groups of the 1st and the 3rd progeny, respectively, than those(OPG 1,658,900) of the challenged control group. The body weight gains of the 1st and the 3rd progeny groups, the 1st week and the 2nd week after challenge, were higher (2.6g and 155.4g, 11.6g and 168.9g respectively) than those(-85.8g and 63.6g, respectively) of the challenged control group, and the feed conversion ratios(FCR 3.28 and 2.96) of the 1st and the 3rd progeny groups were lower than that(FCR 5.60) of the groups challenged control group. The anticoccidial indices(70.5 and 93.9) of the groups challenged with the virulent oocysts of E tenella after immunization with the 1st and the 3rd progeny of the ${\gamma}-irradiated$ E tenella were significantly higher than that (ACI -81.9) of the challenged control group. It was thought that the immunogenicity of ${\gamma}-irradiated$ E tenella would be increase according to increase the number of generation passaged in chicken. That might be because of increasing the pathogenicity of ${\gamma}-irradiated$ E tenella according to increase the number of generation passaged in chicken.

• Korean Journal of Veterinary Research
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• v.33 no.4
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• pp.649-655
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• 1993

A series of experiments on the effects of ${\gamma}-irradiation$ was performed to reveal the pathogenicity of ${\gamma}-irradiated$ oocysts of E tenella from Cobalt-60 and its progeny. The SPF chickens were inoculated with differnt doses of radiation and inoculum. The level of 100 Gy ${\gamma}-irradiation$ from $^{60}Co$ and the level of inoculum with $1{\times}10^4$ oocysts were recognized more pathogenic than those of the other groups by comparison of body weight gains, blood in feces and lesion scores. The signs of blood in feces, lesion score and the number of excreted oocysts in the feces were revealed as the lowest in the group of the ${\gamma}-irradiated$ oocysts, the average in the group of the 1st and the 3rd progeny, and the highest in the group of non-irradiated oocysts of E $tenell\grave{a}$. The body weight gain of the group immunized with ${\gamma}-irradiated$ oocysts of E tenella was higher than those of the non-irradiated, the 1st and 3rd progeny groups. The body weight gain of the groups immunized with the 1st and the 3rd progeny of E tenella were higher than that of the non-irradiated group. The feed conversion ration of the group immunized with ${\gamma}-irradiated$ oocysts of E tenella was lower than those of the non-irradiated, the 1st and the 3rd progeny groups. The feed conversion ratios of the group immunized with the 1st and 3rd progeny of ${\gamma}-irradiated$ oocysts were lower than that of the group infected with non-irradiated E tenella. The anticoccidial index(ACI 190.6) in the chickens immunized with the ${\gamma}-irradiated$ oocysts of E tenella and those(ACI 142.8 and 107.4) of the 1st and the 3rd progeny groups were higher than that (ACT 87.4) of the group infected with non-irradiated E tenella. It was thought that the pathogenicity of ${\gamma}-irradiated$ E tenella would be recovered according to increase the number of generation passaged in chicken.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.382-392
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• 2012

This study evaluated the effects of dietary anticoccidial drugs plus antibiotic growth promoters (AGPs) on parameters of immunity in commercial broiler chickens. Day-old chicks were raised on used litter from a farm with endemic gangrenous dermatitis to simulate natural pathogen exposure and provided with diets containing decoquinate (DECX) or monensin (COBN) as anticoccidials plus bacitracin methylene disalicylate and roxarsone as AGPs. As a negative control, the chickens were fed with a non-supplemented diet. Immune parameters examined were concanavalin A (ConA)-stimulated spleen cell proliferation, intestine intraepithelial lymphocyte (IEL) and spleen cell subpopulations, and cytokine/chemokine mRNA levels in IELs and spleen cells. ConA-induced proliferation was decreased at 14 d post-hatch in DECX-treated chickens, and increased at 25 and 43 d in COBN-treated animals, compared with untreated controls. In DECX-treated birds, increased percentages of $MHC2^+$ and $CD4^+$ IELS were detected at 14 d, but decreased percentages of these cells were seen at 43 d, compared with untreated controls, while increased $TCR2^+$ IELs were evident at the latter time. Dietary COBN was associated with decreased fractions of $MHC2^+$ and $CD4^+$ IELs and reduced percentages of $MHC2^+$, $BU1^+$, and $TCR1^+$ spleen cells compared with controls. The levels of transcripts for interleukin-4 (IL-4), IL-6, IL-17F, IL-13, CXCLi2, interferon-${\gamma}$ (IFN-${\gamma}$), and transforming growth factor${\beta}$4 were elevated in IELs, and those for IL-13, IL-17D, CXCLi2, and IFN-${\gamma}$ were increased in spleen cells, of DECX- and/or COBN-treated chickens compared with untreated controls. By contrast, IL-2 and IL-12 mRNAs in IELs, and IL-4, IL-12, and IL-17F transcripts in spleen cells, were decreased in DECX- and/or COBN-treated chickens compared with controls. These results suggest that DECX or COBN, in combination with bacitracin and roxarsone, modulate the development of the chicken post-hatch immune system.