• 한국균학회지
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• 제23권1호통권72호
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• pp.46-52
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• 1995

화경버섯의 자실체로부터 PAGE로 한 렉틴을 정제하고, LJAP(Lampteromyces japonicus antibacterial protein)라 이름 붙였다. LJAP는 17-kDa의 서브유닛트가 모여서 된 백 kDa이 넘는 회합 단백질이였다. 이 단백질은 아미노산 분석결과 세린, 글리신, 산성 아미노산의 함량이 높았다. LJAP는 대장균중 특히 JM 109, K 12, HB 101, JW 380에 대한 항세균작용이 뛰어났다. 한편, 항세균활성은 당단백질인 asialofetuin에 의하여 크게 억제되었다. 본 당단백질은 화경버섯에서 처음으로 얻어진 항세균활성과 적혈구 응집활성을 가진 렉틴이다.

• 한국자기공명학회논문지
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• 제13권2호
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• pp.96-107
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• 2009

Lactophoricin (LPcin-I) is a cationic amphipathic peptide with 23-mer peptide, and corresponds to the carboxy terminal 113-135 region of Component-3 of proteose-peptone. LPcin-I is a good candidate as a peptide antibiotic, because it has an antibacterial activity, but no hemolytic activity. On the other hand, its shorter analog (LPcin-II), which corresponds to the 119-135 region of PP3, has no antibacterial activity. In order to understand the structure-activity relationship under the membrane environments, we succeed to produce large amounts of LPcin-I and LPcin-II peptides. Peptides were over expressed in the form of fusion protein in Escherichia coli, and purified with several chromatography techniques. In this paper, we introduce the optimizing processes of purification and NMR measurement.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.102-102
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• 2019

Soybean curd residue (SCR), known as a major waste product of soybean processing, is the water-insoluble fraction which is removed by filtration during soymilk production. For these reasons, SCR was usually considered as food waste. SCR might have a good potential as a functional food material, value-added processing and utilization. SCR contains high-quality protein and consists of a good source of nutrients, including protein, oil, dietary fiber, minerals, along with un-specified monosaccharides and oligosaccharides. Also, SCR might be a potential source of low cost protein for human consumption. However, SCR could be a source of bacterial contamination when during food processing. This study was aimed to investigate antibacterial capacity of natural product through detecting relationship between SCR and microbial. We isolated five bacterial strains from SCR and elucidated antibacterial activity of nature medicines to extend storage capacity of food made with SCR. Thus, the extract which showed antibacterial effects in Corynebacterium calloonae and Raoultella amithinolytica is a combination of seven kinds of extracts: Glycyrrhiza uralensis, Cudrania tricuspidata, Salvia miltiorrhiza Bunge, blueberry, Acorus gramineus, Ginkgo biloba L., Camellia sinensis. This study suggested that antibacterial activities of natural medicines could be used for extension of storage capacity in SCR-contained food.

• 한국수산과학회지
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• 제54권6호
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• pp.927-937
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• 2021

This study was performed to screen the antimicrobial activities of the extract from the Pacific oyster Crassostrea gigas against skin pathogens and to purify the relevant antibacterial peptide. The acidified extract showed potent antibacterial activities against gram-positive and gram-negative bacteria but showed no activity against Candida albicans and no significant cell toxicity. Among acne-causing pathogens, the acidified extract showed potent antibacterial activity only against Staphylococcus aureus, and its antibacterial activity was completely abolished by treatment with trypsin or chymotrypsin, and was inhibited by salt treatment. The acidified extract showed strong DNA-binding ability but did not show bacterial membrane permeabilizing ability. Based on antimicrobial activity screening and cytotoxic effects, a novel antibacterial peptide was purified from the acidified gill extract using solid-phase extraction, cation-exchange, and reversed-phase HPLC. The resulting peptide had a molecular weight of 4800.8 Da and showed partial sequence homology with the carbonic anhydrase 4 (CA4) protein in the hard-shelled mussel. Overall, we purified a novel antibacterial peptide, named cgCAFLP, which is related to carbonic anhydrase 4 (CA4) protein, against skin pathogens. Our results suggest that the Pacific oyster extract could be used as an additive to control some acne-related skin pathogens (S. aureus).

• International Journal of Industrial Entomology and Biomaterials
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• 제26권2호
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• pp.119-123
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• 2013

The insect baculovirus expression vector system (BEVS) is useful for producing biologically active recombinant proteins. However, the overexpression of heterologous proteins using this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we developed a versatile baculovirus expression and secretion system using Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion was found to improve the secretions and antibacterial activities of recombinant nuecin and enbocin proteins. Thus, we conclude that bPDI gene fusion is a useful addition to BEVS for the large-scale production of bioactive recombinant proteins.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.26-35
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• 2017

The hydrolysis of proteins constitutes an invaluable tool, granting access to a variety of peptide fragments with potentially interesting biological properties. Therefore, a hemoglobin (Hb) hydrolysate of Crocodylus siamensis was generated by digestion under acidic conditions. The antibacterial and antioxidant activities of the Hb hydrolysate were assessed in comparison with intact Hb. A disc diffusion assay revealed that the Hb hydrolysate exhibited antibacterial activity against eight strains of gram-positive bacteria and showed a higher efficacy than intact Hb. Moreover, the antioxidant activity of intact Hb and its hydrolysate was evaluated using ABTS and DPPH radical scavenging assays. The Hb hydrolysate exhibited free radical scavenging rates of 6-32%, whereas intact Hb showed a slightly higher activity. In addition, non-toxicity to human erythrocytes was observed after treatment with quantities of Hb hydrolysate up to $10{\mu}g$. Moreover, active fragmented Hb (P3) was obtained after purifying the Hb hydrolysate by reversed-phase HPLC. Scanning electron microscopy demonstrated the induction of bacterial cell membrane abnormalities after exposure to P3. Antibacterial and antioxidant activities play crucial roles for supporting the wound healing activity. Consequently, an in vivo mice excisional skin wound healing assay was carried out to investigate the effects of intact Hb treatment on wound healing in more detail. The results clearly demonstrate that intact Hb is capable of promoting 75% wound closure within 6 days. These findings imply that intact Hb of C. siamensis and its acid hydrolysate may serve as valuable precursors for food supplementary products benefitting human health.

• 한국잠사곤충학회지
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• 제44권2호
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• pp.64-68
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• 2002

본 연구는 곤충 유전자를 이용한 항세균성 펩타이드 생산 및 농업용 소재로서의 응용에 관한 연구로서 항세균성 단백질 누에신 유전자를 베큘로 바이러스 발현계(BEVS)를 이용하여 곤충세포주에서 발현한 후 누에신 단백질의 농업용 소재로서의 가능성을 모색하기 위해 농작물을 가해하는 감자 고추의 무름병을 일으키는 Pectobacterium carotovorum subsp. carotovorum, 가지 및 고추의 풋마름병을 일으키는 Ralstonia solanacearum, 양송이 버섯의 세균성 갈색 무늬 병을 일으키는 Pseudomonas tolaasii 및 무와 배추의 검은썩음병을 일으키는 Xanthomonas campestris pv. campestris 에 대해서 항세균 활성을 관찰하였다. 그 결과 Pectobacterium carotovorum subsp. carotovorum, Ralstonia solanacearum 및 Pseudomonas tolaasii에 대해 높은 활성을 나타내었으며 Xanthomonas campestris pv. campestris에는 활성을 나타내지 않았다. Ion exchange 및 gel filtration chromatography 를 수행하여 약 20 kDa의 성숙 누에신 단백질을 순수 분리하여 pH 및 온도에 대한 안정성을 조사한 결과, pH 2~12 완충액에서 30분간 처리하였을 때에도 항세균 활성이 그대로 유지되었고 10$0^{\circ}C$에서 2시간 처리시에는 활성이 안정되었으며 4시간 처리시에도 80% 정도로 유지됨을 확인함으로써 누에신 단백질은 pH 및 온도에 대한 안정성이 있음을 확인할 수 있었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권2호
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• pp.231-238
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• 2011

Attacin is an insect antibacterial protein that plays an important role in immune response to injury and infection. In this report, we have isolated and characterized of cDNA encoding for the attacin from the immunized larvae of swallowtail butterfly, $Papilio$ $xuthus$. A full length cDNA of $P.$ $xuthus$ attacin was obtained by employing annealing control primer (ACP)-based differential display PCR and 5' RACE. The complete $P.$ $xuthus$ attacin cDNA was comprised of 949 bp encoding a 250 amino acid precursor. It contains a putative 18 amino acid signal peptide sequence, a 42 amino acid propeptide sequence, and a 190 amino acid mature protein with a theoretical molecular mass of 19904.01 and a pI of 9.13. The putative mature protein of $P.$ $xuthus$ attacin showed 48-52% and 24-30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. Semiquantitive RT-PCR results revealed that the transcript of $P.$ $xuthus$ attacin gene was up-regulated at significant levels after injection with bacterial lipopolysaccharide (LPS). We sub-cloned cDNA fragment encoding mature $P.$ $xuthus$ attacin into the expression vector, highly expressed in $E.$ $coli$ BL21 cells, and its antibacterial activity was analyzed. Recombinant $P.$ $xuthus$ attacin evidenced considerably antibacterial activity against Gram-negative bacteria, $E.$ $coli$ ML 35 and $Klebsiella$ $pneumonia$.

• 한국동물학회지
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• 제38권3호
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• pp.305-312
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• 1995

배추흰나비 5령 유충의 복부에 E. coli K-12를 주입한 다음 면역혈림프로 부터 다섯 종류의 항균단백질을 유도하였으며, 이러한 단백질들은 그람 양성균과 그람 음성균에 대해 항균성이 있거나 혹은 그람 양성균에 대해서만 항균성이 있었다. 유도된 단백질 가운데 열에 안정되고 염기성인 4 kDa 정도의 분자량을 갖는 peptide를 분리하였으며, 이것을 hinnavins이라고 명명하였다.

• 한국임상수의학회지
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• 제15권1호
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• pp.36-40
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• 1998

This experiment was carried out in order to evaluate the immunomodulatory activity of levamisole (LMS) in 5. fgrjn challenged eels with different treatment regimens: 7-day LMS treatment before the challenge, 7-day LMS treatment started simultaneously with the challenge, 14-day treatment before and after the challenge. The antibacterial effect was activated in all treated groups, with the best being obtained in the simultaneously treated group. LMS stimulated the defense mechanisms of the eel as demonstrated by increase in the level of total protein, albumin, trypsin inhibitor capacity, lysozyme activity, antibody titers antibacterial activity and survival rate. These results suggest that antibacterial effects of LMS was achieved by not only non-specific immune response but also specific one in eel.