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Characterization and Expression of Antibacterial Protein Gene, Nuecin  

윤은영 (농촌진흥청 농업과학기술원 잠사곤충부)
구태원 (농촌진흥청 농업과학기술원 잠사곤충부)
황재삼 (농촌진흥청 농업과학기술원 잠사곤충부)
김상현 (농촌진흥청 농업과학기술원 잠사곤충부)
강석우 (농촌진흥청 농업과학기술원 잠사곤충부)
김근영 (농촌진흥청 농업과학기술원 잠사곤충부)
진병래 (동아대학교 생명자원과학부)
Publication Information
Journal of Sericultural and Entomological Science / v.44, no.2, 2002 , pp. 64-68 More about this Journal
Abstract
The antibacterial protein gene, nuecin was expressed in Sf9 cells using baculovirus expression vector system (BEVS). The antibactetial activity of mature nuecin against Pectobacterium carotovorum subsp. carotovorum, Ralstonia solanacearum and Pseudomonas tolaasii was significantly high, demonstrating that nuecin had a wider antibacterial spectrum on gram negative and positive bacteria. The result appears to be superior to other antibacterial peptide, attacin. The nuecin was purified by SP-sepharose and Mono Q HR ion-exchange chromatography, and then by Superdex 200 HR 10/30 column. The purified nuecin is quite stable at 80$\^{C}$ and 100$\^{C}$ for several hours of incubation and in a wide pH range (pH 2-12).
Keywords
BEVS (baculovirus expression vector system); antibacterial protein gene; nuecin;
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Times Cited By KSCI : 2  (Citation Analysis)
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