• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.189-196
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• 2021

Periplanetasin-2 from cockroach exhibits broad-spectrum antimicrobial activity. The underlying antibacterial mechanisms rely on the stimulation of reactive oxygen species overproduction to induce apoptotic cell death. A promising strategy to increase the bioavailability of periplanetasin-2 involves reducing the dose through combination therapy with other antibacterials that show synergistic effects. Thus, the synergistic antibacterial activity of periplanetasin-2 with conventional antibacterial agents and its mechanisms was examined against Escherichia coli in this study. Among the agents tested, the combinations of periplanetasin-2 with vancomycin and chloramphenicol exhibited synergistic effects. Periplanetasin-2 in combination with vancomycin and chloramphenicol demonstrated antibacterial activity through the intracellular oxidative stress response. The combination with vancomycin resulted in the enhancement of bacterial apoptosis-like death, whereas the combination with chloramphenicol enhanced oxidative stress damage. These synergistic interactions of periplanetasin-2 can help broaden the spectrum of conventional antibiotics. The combination of antimicrobial peptides and conventional antibiotics is proposed as a novel perspective on treatments to combat severe bacterial infection.

• Journal of Applied Biological Chemistry
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• 제64권2호
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• pp.133-140
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• 2021

본 연구에서는 개복숭아꽃과 흰색, 분홍색 매화 꽃의 항균 활성과 항산화, 미백 효과에 대해 알아보고자 하였다. 먼저, 3종의 꽃추출물들은 4종의 균주 중에 S. aureus에 대해 우수한 항균 활성을 나타냈다. 흰매화 클로로포름층은 0.5 mg/mL 농도에서 S. aureus에 대해 84%의 항균 활성을 보였고 개복숭아꽃과 분홍매화 클로로포름층도 0.5 mg/mL 농도에서 각각 49, 30%의 항균 효과를 나타냈다. 3종의 꽃추출물들은 P. aeruginosa에 대해서도 항균 효과를 보였다. 개복숭아꽃 클로로포름층과 분홍매화 클로로포름층은 0.5 mg/mL 농도에서 각각 36, 30%의 항균 활성을 보였다. 이 추출물들은 S. epidermidis와 E. coli에 대해서는 항균 활성을 보이지 않았다. 3종의 꽃추출물들은 HaCaT cell의 생존에 크게 영향을 미치지 않았다. 분홍매화 증류수층은 20, 40 ㎍/mL 농도에서 항산화 효과를 보였다. 분홍매화 에틸아세테이트층도 40 ㎍/mL에서 항산화 효과를 보였는데 이는 글루타치온 보다 우수하였다. 꽃추출물들은 B16F10 cell의 생존율에는 영향을 미치지 않았다. 꽃추출물들의 미백 효과는 개복숭아꽃 클로로포름층 40 ㎍/mL에서 18%가 나타났다. 이러한 결과들을 바탕으로 3종의 꽃추출물들은 항균 활성과 항산화, 미백 효과가 있는 원료로 판단할 수 있었다.

• 대한수의학회지
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• 제54권3호
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• pp.189-192
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• 2014

This study evaluated the antibacterial effects of a mixture of Sophorae radix and Glycyrrhiza uralensis Fischer (1 : 1) ethanol extracts (SGE) on mice infected with Streptococcus (S.) pyogenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of SGE necessary for antibacterial effects against S. pyogenes were $20{\mu}g/mL$. Based on the time-kill curves for S. pyogenes, SGE was effective at $4{\times}$ MIC after 16 h. On Day 12 after challenge, the survival rate of mice treated with 2.0 mg/kg SGE was 60%. In conclusion, SGE had potent in vitro and in vivo antibacterial activities against S. pyogenes.

• 한국자원식물학회지
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• 제30권3호
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• pp.311-317
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• 2017

This study was conducted with the aim of evaluating the antibiotic effects of leaves, stems, and roots in yacon (Smallanthus sonchifolius). Antibacterial activity of leaf extract by disk diffusion method with Bacillus subtilis and Escherichia coli respectively showed 13.3 and 13.75mm diameters of clear zone. There was no significant difference between the stems and leaves. The minimum inhibitory concentration of leaves' heating and agitation extraction showed a restrain of strain at 1mg/ml, but the stems and root extract did not appear. Yacon is a functional antibacterial material, and methanol extraction is more effective than water. This research was to investigate the growth stage of collection has the most effective antibacterial effects. It has collected yacon's leaves from June to October, which is an appropriate time for collection right before reaping. Yacon leaf has antibacterial effects on Bacillus subtilis, Escherichia coli, Enterococcus faecium, and Salmonella enteritifis. There were no significant differences by the growth stage of collection. Leaves collected in July are high in phenol which helps in sulfating activity works well considering the high scavenging capability of DPPH. Leaves collected in September are high in total flavonoid.

• 한국축산식품학회지
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• 제36권4호
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• pp.452-457
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• 2016

Lactoferrin is a glycoprotein with various biological effects, with antibacterial activity being one of the first effects reported. This glycoprotein suppresses bacterial growth through bacteriostatic or bactericidal action. It also stimulates the growth of certain kinds of bacteria such as lactic acid bacteria and bifidobacteria. In this study, Asn-Leu-Asn-Arg was selected and chemically synthesized based on the partial sequences of bovine lactoferrin tryptic fragments. Synthetic Asn-Leu-Asn-Arg suppressed the growth of Pseudomonas fluorescens, P. syringae and Escherichia coli. P. fluorescens is a major psychrotrophic bacteria found in raw and pasteurized milk, which decreases milk quality. P. syringae is a harmful infectious bacterium that damages plants. However, synthetic Asn-Leu-Asn-Arg did not inhibit the growth of Lactobacillus acidophilus. It is expected that this synthetic peptide would be the first peptide sequence from the bovine lactoferrin C-lobe that shows antibacterial activity.

• 대한수의학회지
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• 제49권2호
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• pp.135-139
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• 2009

Streptomyces (S.) fradiae is a microbe with broad-spectrum antimicrobial activity, isolated from soil. In the present study, antibacterial effects of S. fradiaea against Salmonella (S.) gallinarum was determined. S. fradiae inhibited growing of S. gallinarum in Luria-Bertani media agar. Moreover, ingestion of S. fradiae markedly inhibited mortality of chickens experimentally infected with S. gallinarum. There is no side effect by S. fradiaeon, in safety of chickens and antibiotic material residues in chicken meat. Taken together, S. fradiae have the antibacterial effects against S. gallinarum. Therefore, we concluded that S. fradiae might be a good microbial candidate for treatment or control of fowl typhoid in chickens.

• 대한화장품학회지
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• 제44권1호
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• pp.59-66
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• 2018

Propionibacterium acnes (P. acnes)는 여드름의 주요 병원성균으로 염증성 질환에 관련된 미생물 중에 하나이다. 본 연구에서는 여드름 주요 유발 균주인 P. acnes에 대하여 70% Ethanol로 추출한 제주 자생식물 추출물의 항균활성을 disc diffusion test 실험을 이용하여 측정하였다. 실험 결과 61개의 제주 자생 식물 추출물 중에서 45개 추출물의 항균 활성이 검출되었으며, 16개의 추출물은 항균 활성이 검출되지 않았다. 그중에서 여우 구슬(Phyllanthus urinaria L.) - 줄기 잎 추출물이 $18.96{\pm}0.69mm$로 가장 높은 항균 활성을 나타내었고, 참당귀(Angelica gigas Nakai) - 뿌리, 돌외(Gynostemma pentaphyllum (Thunb.) Makino), 참당귀(Angelica gigas Nakai) - 잎 줄기, 산박하(Isodon inflexus (Thunb.) Kudo), 차나무(Camellia sinensis L.) - 꽃, 그리고 로즈마리(Rosmarinus officinalis L.) - 줄기 추출물 순으로 높은 항균 활성을 나타내었다. 이러한 결과는 제주 자생 식물 추출물이 여드름 치료 예방의 항균제 및 기능성 화장품 천연 원료로써의 이용 가능성을 제안한다.

• 한국결정성장학회지
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• 제12권4호
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• pp.165-171
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• 2002

피치계 탄소섬유로부터 활성탄소섬유를 제조한 후, 이에 대하여 금속를 처리하여 금속-ACFs를 제조하였다. 제조된 금속-ACFs에 대하여 흡착 및 표면특성을 통하여 물리화학적 특성을 제시하였다. BET식으로부터 금속이 처리된 활성 탄소섬유의 비표면적을 구한 결과 Ag-ACF의 경우 136.2~1585$m^2$/g의 범위에, Cu가 처리된 활성탄소섬유에 경우 698.2~896.2$\m^2$/g의 범위에, Ni-ACF의 경우 685.2~898.2$\m^2$/g의 범위에 분포하였다. 또한 $\alpha_s$-법을 사용하여 구한 미세 동공부피는 Ag-ACF에 대하여 0.07~1.2cm^3/g, Cu-ACF에 대하여 0.2~0.3cm^3/g, Ni-ACF에 대하여 0.3~0.7cm^3/g, 의 범위에 분포함을 알 수 있었다. 이와 관련하여 SEM 분석을 통하여 표면성상에 대하여 연구한 결과, 처리된 금속은 섬유표면 주위를 부분적으로 또는 전 범위에 걸쳐서 피복되어 있음이 관찰되었다. 최종적으로 Shake 플라스크법에 의거하여 대장균(E. coli)에 대한 금속-ACFs의 항균특성에 대한 결과로부터 금속의 양이 증가함에 따라 항균효과가 크게 증가함을 알 수 있었다.

• 대한약침학회지
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• 제9권3호통권21호
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• pp.97-104
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• 2006

Objectives : This study was conducted to compare antibacterial activities and free radical scavenging activity between the Bee Venom and Sweet Bee Venom in which the allergy-causing enzyme is removed. Methods : To evaluate antibacterial activities of the test samples, gram negative E. coli and gram positive St. aureus were compared using the paper disc method. For comparison of the antioxidant effects, DPPH(1,1-diphenyl-2picrylhydrazyl) free radical scavenging assay and Thiobarbituric Acid Reactive Substances(TBARS) assay were conducted. Results : 1. Antibacterial activity against gram negative E. coli was greater in the Sweet Bee Venom group than the Bee Venom group. 2. Antibacterial activity against gram positive St. aureus was similar between the Bee Venom and Sweet Bee Venom groups. 3. DPPH free radical scavenging activity of the Bee Venom group showed 2.8 times stronger than that of the Sweet Bee Venom group. 4. Inhibition of lipid peroxidation of the Bee Venom group showed 782 times greater than that of the Sweet Bee Venom group. Conclusions : The Bee Venom group showed outstanding antibacterial activity against gram positive St. aureus, and allergen-removed Sweet Bee Venom group showed outstanding antibacterial activity against both gram negative E. coli and gram positive St. aureus. For antioxidant effects, the Bee Venom was superior over the Sweet Bee Venom and the superiority was far more apparent for lipid peroxidation.

• Bulletin of the Korean Chemical Society
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• 제25권5호
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• pp.639-646
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• 2004

Metal treated activated carbons are prepared using various metals. Adsorption behaviors, morphologies, as well as antibacterial effects of metal treated samples are compared before and after solvent washing. Adsorption isotherms are used to characterize the porous structure of metal treated activated carbons before and after the solvent washing with acetone or ethyl alcohol. From these data, it is noticed that the changes in physicochemical properties of metal treated activated carbons depend on the solvents employed. Similar results are observed from BET data obtained from nitrogen adsorption isotherms. From scanning electron microscopy (SEM) studies, the changes in shape and size of metal particles are observed after the samples are washed with solvents. These changes result in different blocking effects, which, in turn, affect the adsorption behavior of metal treated activated carbons. X-ray diffraction (XRD) patterns of the samples treated with different metals are different each other. High intense sharp peaks attributed to metals are observed from silver treated samples, while the peaks are not observed from copper treated samples. To compare thermodynamic behavior of metal treated activated carbons washed with different type of solvents, differential scanning calorimetric (DSC) analysis is carried out. The analysis shows similar endothermic curves for all of the samples. Finally, antibacterial effects of metal treated activated carbon against Escherichia coli are discussed. Comparing the effects among the metals employed, highest effects are obtained from Cd, while lowest effects are obtained from Cu. Antibacterial activity becomes higher with the increase of the amount of metals treated, Optimum concentrations of metals to treat activated carbons, obtained from a shake flask test, are known to be 0.4, 0.1, and 0.6 moles for Ag, Cd, and Cu, respectively.