• Journal of Conservation Science
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• v.36 no.6
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• pp.505-510
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• 2020

In this study, the anti-stain effect of the Traditional Myoung-oil, which has been reproduced through traditional method, the Clean Myoung-oil, which was developed in an eco-friendly method through scientific analysis of Traditional Myoung-oil, and the perilla oil, which is the raw material of Myoung-oil and is currently used as a finishing agent when repairing wooden cultural properties was evaluated. As a result of the evaluation, perilla oil showed almost no anti-stain effect, whereas both types of Myoung-oil showed high anti-stain effect. However, it was confirmed that the anti-stain effect was significantly reduced after 4 weeks of exposure to the strain when Myoung-oil was diluted with terpene oil, a natural solvent. Thus, it was considered that the amount of treatment in the wood affected the anti-stain effect of Myoung-oil. In other words, in constructing wooden buildings, Myoung-o il is more suitable as a finishing agent to suppress mold growth than perilla oil. And, in order to increase the applicability of Myoung-oil, it is suggested that additional research on the optimal treatment amount and treatment method that can inhibit mold growth inhibition in outdoor environments is necessary.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.12
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• pp.39-46
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• 2008

Asymmetric glass lens core for portable projection optic system was designed and simulated. And it was machined by newly developed non-rotational ultra precision grinding method. With the designed lens data which optimized for multi-collimation, we generated the we core surface data. Mold pressing conditions analyzed by FEM. In the machining process, ground profile errors were compensated based on measured data, minimized feed rate and depth of cut. The deviations of machined core profile were acceptable level for glass mold press. Mold pressed glass array lens was coated with $SiO_2\;and\;Ta_2O_5$ for anti-reflection.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.18 no.6
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• pp.697-701
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• 2009

A simple method for the fabrication of porous nano-master for the anti-reflection effect on the transparent substrates is presented. In the conventional fabrication methods for antireflective surface, coating method using materials with low refractive index has usually been used. However, it is required to have a high cost and long processing time for mass production. In this paper, we developed a porous nano-master with anti-reflective surface for the molding stamper of the injection mold, hot embossing and UV imprinting by using the aluminum anodizing process. Through two-step anodizing and etching processes, a porous nano-master with anti-reflective surface was fabricated at the large area. Pattern size Pore diameter and inter-pore distance are about 130nm and 200nm, respectively. In order to replicate anti-reflective structure, hot embossing process was performed by varying the processing parameters such as temperature, pressure and embossing time etc. Finally, antireflective surface can be successfully obtained after etching process to remove selectively silicon layer of AAO master.

• Journal of Life Science
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• v.13 no.2
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• pp.223-229
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• 2003

A study of the anti-fungal properties in Korean radish was conducted using a variety of purification procedures such as Extrelut column, RP(Reverse Phase) Cl8 Column Chromatography, HPLC etc. to separate anti-fungal substances from Korean radish juices to test them against a common gray mold called Botrytis cenerea. Dialysis tube operation showed that these substances were presumably thermostable compounds with low molecular mass (less than 3.5 kDa). Differences of anti-fungal activities depending upon types of radishes used did not show any noticeable variation. The antifungals were presumably composed of more than 5 compounds. Among these, the most anti-fungal fraction was analyzed by HPLC in which one peak was obtained. Disease-affected plants were inoculated with 10mg of Extrelut fraction and results showed similar anti-fungal activity to pesticides suggesting possible usage of these substances as environmentally friendly antibiotics.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2002.04a
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• pp.22-27
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• 2002

The existing compressor steel seal used in automotive air-conditioner has the problem of oil leakage and the deterioration in shielding performance, due to the abrasion and the corrosion of the material. A new type of polymer resin seal is studied in the research. The polymer resin seal has the characteristics of high anti-abrasiveness and anti-corrosiveness, which can overcome the deflects of the steel seal. In addition, the seal needs lower manufacturing cost and is appropriate to mass production, because it is made by the injection molding method requiring no mechanical processing. The profile generation program for seal mold has been developed using the gradient method, and the molding characteristics of the seal have analyzed through the flow analysis and the warpage analysis. The program has been verified by comparing the analysis results with the measured data of the test product. The research might be said to provide the basic method to produce the polymer resin seals with various types and dimensions.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.1028-1032
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• 1995

This paper describes the manufacture of spherical and aspherical surface on glass, superalloy and ceramic components. The rotationally symmetricallenses, and the ceramic or superalloy molds with spherical shapes are mainly generated by cutting processes on CNC lathe machine or 4,5 axis CNC machining centers. Recently, spherical shape parts require more precise and efficent machining technologies for wide material range such as optical lens of the lithography device in semiconductor manufacturing processes or the high precision mold machining of anti-chemical, anti-wear materials. In this paper, we introduce a newly developed infeed grinding machine with metal with metal bonded cup type wheel and its effects on spherical surface grinding.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.11 no.5
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• pp.81-87
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• 2002

The existing compressor steel seal used in automotive air-conditioner has the problem of oil leakage and deterioration in shielding performance, due to the abrasion and corrosion of the material. A new type of polymer resin seal studied in this research has the characteristics of high anti-abrasiveness and anti-corrosiveness, which can oversome the defects of the steel seal. In addition, the seal needs lower manufacturing cost and is appropriate to mass production, because it is made by the injection molding method requiring no mechanical processing. The profile generation program for seal mold has been developed using the gradient method, and the molding characteristics of the seal have analyzed through the flow analysis and the warpage analysis. The program has been verified by comparing the analysis results with the measured data of the test product. The research might be said to provide the basic method to produce the polymer resin seals with various types and dimensions.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.149-153
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• 2017

Contamination and growth of Trichoderma, a green mold, on the oak log and wooden chip or sawdust media can severely inhibit the growth of oak mushroom. Chemicals including pesticides and antibiotics are generally not allowed for the control of green mold disease during mushroom cultivation. In this study, bacterial pathogens causing blotch disease on the oyster mushrooms were isolated and their peptide toxins were purified for the control of green mold disease. Strains of Pseudomonas tolaasii secret various peptide toxins, tolaasin and its structural analogues, having antifungal activities. These peptides have shown no effects on the growth of oak mushrooms. When the peptide toxins were applied to the green mold, Trichoderma harzianum H1, they inhibited the growth of green molds. Among the 20 strains of peptide-forming P. tolaasii, strong, moderate, and weak antifungal activities were measured from 8, 5, and 7 strains, respectively. During oak mushroom cultivation, bacterial culture supernatants containing the peptide toxins were sprayed on the aerial mycelia of green molds grown on the surface of sawdust media. The culture supernatants were able to suppress the fungal growth of green molds while no effect was observed on the mushroom growth and production. They changed the color of molds from white aerial mycelium into yellowish dried scab, representing the powerful anti-fungal and sterilization activities of peptide toxins.

• Korean Journal of Environmental Agriculture
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• v.28 no.4
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• pp.435-441
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• 2009

In the present study, we analyzed the defensin protein deduced from Korean radish (Raphanus sativus L.) seeds.To express the genes in E. coli, we constructed a recombinant expression vector with a defensin gene, named rKRs-AFP gene isolated from Korean radish seeds. Over expressed rKRs-AFP proteins was separated by SDS-PAGE to determine the purity, and protein concentration was determined by the Bradford method. Antifungal activity was assessed by disk assay method against the tested fungi. As a result, when 500 mL of cell culture were disrupted by sonicator, 32.5 mg total proteins were obtained. The purified protein showed a single band on SDS-PAGE with estimated molecular weight about 6 KDa, consistent with the molecular mass calculated from the deduced amino acid sequence. The purified rKRs-AFP protein showed remarkable antifungal activities against several fungi including Aspergillus niger, Botrytis cinerea causing the gray mold disease, and Candida albicans. In field tests using the purified rKRs-AFP protein, the protein showed the reducing activity of disease spot and the mitigating effect of spreading of disease like agrichemicals. The immuno-assay of rKRs-AFP protein showed that the purified protein entirely accumulated at B. cinerea cytoplasm through the hyphal septa shown by fluorescence imaging. There was no fluorescence inside the cell, when the hypha was incubated without the protein. These all results indicate that the recombinant rKRs-AFP proteins can be utilized as a potential antifungal drug to control harmful plant fungal pathogens.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.5
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• pp.337-342
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• 2008

Eight quality standards of the brown seaweed, Undaria pinnatifida were designed and evaluated with dry products collected from aquacultured and wild areas. Contents of n-3 fatty acids (stearidonic acid and eicosapentaenoic acid), anti-edema activity, and total carbohydrate were measured as functional criteria. Total bacteria and molds were counted for safety conditions. Amounts of a brown pigment fucoxanthin, debris, and moisture were measured to evaluate the visible status of the products. When the seaweeds were tested in 1 g dry weight basis, they showed n-3 fatty acids in the range of approximately 0.2-2.8 mg, total carbohydrate of 0.39-0.71 g, total bacteria of $10^{4-6}$ cells, mold of $10^2$ cells, fucoxanthin of 0.4-1.5 mg, and debris of 10-24 mg. Anti-edema activity was 0.03-0.47 AU at 40 mg/mL extract. Moisture content was 11.4-12.6%.