• Journal of Microbiology and Biotechnology
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• 제1권4호
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• pp.232-239
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• 1991

Caboxymethyl cellulase (CMCase) I was purified from the induced culture filtrate of Penicllium verruculosum F-3 by ammonium sulfate precipitation, DEAE-Sephadex A-50 chromatography and Bio-gel P-150 filtration. The purified enzyme was assumed to be a glycoprotein consisting of 8.5% carbohydrate and having a molecular weight of 70.000 in SDS-polycrylamide gel electrophoresis (SDS-PAGE). The purified enzyme-specific anti-CMCase I IgG was obtained by rabbit immunization and protein A-sepharose CL-4B chromatography. The fungal poly($A^+$) RNA was isolated from the total RNA of the mycelium grown under cellulase induction conditions by oligo(dT)-cellulosse chromatography. The translation products in vitro were prepared by translating the isolated poly ($A^+$) RNA in rabbit reticulocyte lysate and analyzed by SDS-PAGE and fluorography. Of the translation products, CMCase I was identified by the immunoprecipitation against anti-CMCase I IgG.

• Natural Product Sciences
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• 제12권3호
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• pp.162-165
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• 2006

Curcuma aeruginosa Roxb. (FamilyZingeberaceae), commonly known as 'pink and blue giner' is widely used in Indian system of medicine since time immemorial. The plant is found well in wild habitat and cultivated in southern India. The plant is distinguished by red corolla lobes and ferruginous or greenish-blue rhizome. Rhizoma is used medicinally as anti-diarrheal, anti-fungal; external use as astringent for wounds. A detailed pharmacognostic evaluation of its rhizome showed total ash 6.1%, acid insoluble ash 1.20%, alcohol soluble extractives 3.70% , water soluble extractives 14.50%, sugar 20.93%, starch 41.85%, and tannins 0.68%. Oh hydro distillation, the rhizomes and sessile tubers yielded 0.17% oil.

• 임산에너지
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• 제19권1호
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• pp.49-60
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• 2000

본 실험에서는 3종의 한국산 겨우살이 메탄올 추출물을 유기용매 분획과 컬럼크로마토그래피를 이용한 분획을 실시한 후, Tyromyces palustris, Endothia nischkei 그리고 Trichophyton rubrum에 대한 항균활성을 평가하여 겨우살이 추출물의 항균활성 자료로 활용하고자 하였다. 그 결과 모든 겨우살이 추출물은 갈색 불후균에 대한 활성은 매우 낮게 나타났다. 그러나 Viscum albumvar. coloratum 추출물중 부탄올 분획은 황색줄기 마름 병원균의 성장을 억제하는 것으로 나타났으며, 이 겨우살이의 EtOAC 분획은 족부백선균에 대하여 높은 포자 발아 억제효과를 나타내었다. 시험된 3종의 한국산 경우살이류 중 V. album var. Coloratum은 병원균에 대하여 황균활성 성분을 함유할 가능성이 가장 높을 것으로 제시되었다.

• Journal of the Korean Wood Science and Technology
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• 제32권2호
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• pp.50-57
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• 2004

본 실험은 건조되지 않은 목재를 고온의 아마인유에 침지처리함으로써 목재건조와 동시에 발수효과를 얻고자 실시하였다.함수율 90% 이상의 소나무생재를 온도 150℃의 아마인유에서 6시간 침지처리하였을 때 목재함수율은 10%까지 떨어졌으며, 목재 축방향의 깊이에 관계없이 목재단면의 20%에 해당하는 아마인유가 침투하였다. 이때 목재의 강도적 손실은 발생하지 않는 것으로 나타났다. 목재표면의 천공처리는 목재의 내부할열방지에 효과적이었고, 아마인유의 깊은 침투를 유도할 수 있었다. 흡수성 시험 결과 처리재는 무처리재에 비하여 높은 발수효과를 나타냈다. 변색균 및 부후균에 대한 항균효력시험은 처리재 자체로서 균에 대한 독성 기작이 없었으나, 백색부후균 및 갈색부후균에 대해서는 무처리재보다 낮은 중량감소율을 나타내는 등 방부효력의 상승이 있었다.

• 동의신경정신과학회지
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• 제10권2호
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• pp.47-57
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• 1999

Recently, essential oils are used for aromatherapy. Most essential oils are said to be anti-bacterial; some may be anti-viral or anti-fungal. I investigated the effects of peppermint pure essential oil on the heat shock-induced apoptosis in human astrocyte cell line CCF-STTGI. In previous studies, heat shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. We studied the heat shock-induced apoptosis through flow cytometry, DNA electrophoresis, and giemsa staining. Interestingly, these events were inhibited by pretreatment of peppermint pure essential oils in CCF-STTGl cells. Peppermint oil also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. In addition, this Peppermint essential oil inhibited the heat shock-induced activation of caspase-3. These results suggest that peppermint pure essential oils may modulate the apoptosis through the activation of the interleukin-I -converting enzyme-like protease.

• Advances in Traditional Medicine
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• 제8권4호
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• pp.400-407
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• 2008

The aim of the present study is to investigate the anti-inflammatory and antimicrobial activity of petroleum ether and ethanol extracts of Scutia myrtina (Family: Rhamnaceae). In anti-inflammatory activity carrageenan and histamine induced paw oedema and cotton pellet induced granuloma for acute and chronic inflammatory models were studied in Wister albino rats. Based on the results of the present study it can be concluded that petroleum ether and ethanol extract of Scutia myrtina at 400 mg/kg has potential anti-inflammatory effect and they act in a dose dependent manner. Both extracts of Scutia myrtina showed antimicrobial activity against all bacterial and fungal strains tested at the concentration of $100\;{\mu}g$/ml. From the result, it can be concluded that the Scutia myrtina contain antibacterial and antifungal principle. Further more, besides the confirmation of the popular use; the obtained results demonstrate this herbal drug to represent a new source of antimicrobial and anti-inflammatory agent.

• Journal of the Korean Wood Science and Technology
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• 제49권4호
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• pp.371-383
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• 2021

Plant essential oils are used in products such as fragrances and cosmetics due to their individual aromatic characteristics. Currently, essential oils are not only used in cosmetics but also in pharmaceutical products with anti-bacterial, anti-viral, anti-fungal, anti-parasitic, insecticidal, anti-cancer, neuroprotective, psychophysiological, or anti-aging effects. Despite their pharmaceutical properties, some studies reported cytotoxic effects in high doses. Therefore, for pharmaceutical purposes, the margin of safety of essential oils needs to be examined. Herein, we evaluated the IC₅₀ of 10 essential oil from Korean native plants: Juniperus chinensis L. var. sargentii Henry, Citrus natsudaidai Hayata, Citrus reticulata Blanco, Citrus unshiu (Yu. Tanaka ex Swingle) Marcow, Artemisia capillaris Thunb, Aster glehnii F. Schmidt, Juniperus chinensis L, Zanthoxylum schinifolium Siebold & Zucc, Zanthoxylum piperitum (L.) D, and Cinnamomum loureirii. In addition, gene regulation of the cell-cycle gene and apoptosis marker CASP3 was examined at the IC₅₀ level. The purpose of this study was to describe the toxic concentrations of essential oils extracted from Korean native plants, thereby providing toxic concentration guidelines for inclusion in a toxicity database and in the application of plant essential oils in various fields.

• The Plant Pathology Journal
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• 제20권3호
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• pp.172-176
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• 2004

Bacterial isolates TRL2-3 and TRK2-2 showing anti-fungal activity in vitro test against some plant pathogens were identified as Pseudomonas putida and Micrococcus luteus, respectively. Pre-treatment with both bacterial isolates at the concentration 1.0$\times$ $10^7$ and $10^6$cfu/ml in the rhizosphere could trigger induced systemic resistance in the aerial part of cucumber plants against anthracnose caused by Colletotrichum orbiculare. However, the pre-treatment with the higher concentration at 1.0 $\times$ $10^8$ cfu/ml of both isolates could not induce resistance after challenge inoculation with C. orbiculare. As a positive control, the treatment with DL-3 amino butyric acid caused a remarkable reduction of disease severity whereas the lesions on the leaves of untreated plants developed apparently after the fungal inoculation. From these results, it was recomended that disease control using both bacterial isolates inducing systemic resistance in the field where chemical application is forbid.

• 한국환경농학회지
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• 제28권4호
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• pp.435-441
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• 2009

In the present study, we analyzed the defensin protein deduced from Korean radish (Raphanus sativus L.) seeds.To express the genes in E. coli, we constructed a recombinant expression vector with a defensin gene, named rKRs-AFP gene isolated from Korean radish seeds. Over expressed rKRs-AFP proteins was separated by SDS-PAGE to determine the purity, and protein concentration was determined by the Bradford method. Antifungal activity was assessed by disk assay method against the tested fungi. As a result, when 500 mL of cell culture were disrupted by sonicator, 32.5 mg total proteins were obtained. The purified protein showed a single band on SDS-PAGE with estimated molecular weight about 6 KDa, consistent with the molecular mass calculated from the deduced amino acid sequence. The purified rKRs-AFP protein showed remarkable antifungal activities against several fungi including Aspergillus niger, Botrytis cinerea causing the gray mold disease, and Candida albicans. In field tests using the purified rKRs-AFP protein, the protein showed the reducing activity of disease spot and the mitigating effect of spreading of disease like agrichemicals. The immuno-assay of rKRs-AFP protein showed that the purified protein entirely accumulated at B. cinerea cytoplasm through the hyphal septa shown by fluorescence imaging. There was no fluorescence inside the cell, when the hypha was incubated without the protein. These all results indicate that the recombinant rKRs-AFP proteins can be utilized as a potential antifungal drug to control harmful plant fungal pathogens.

• Journal of Korean Neurosurgical Society
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• 제52권4호
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• pp.420-422
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• 2012

Cerebral aspergillosis is rare and usually misdiagnosed because its presentation is similar to that of a tumor. The correct diagnosis is usually made intra-operatively. Cerebral abscess with fungal infection is extremely rare and few cases have been reported, but it carries a poor prognosis. A 73 year-old man presented with decreased visual acuity and paresis of the right cranial nerve III. Magnetic resonance imaging (MRI) revealed a mass in the right cavernous sinus, extened to the anterior crainial fossa and the superior orbital fissure. During surgery, a well encapsulated pus pocket was found, and histopathological examination of the mass resulted in the diagnosis of aspergillosis. Despite appropriate anti-fungal treatment, the patient eventually died from fatal cerebral ischemic change and severe brain swelling. The correct diagnosis of cerebral aspergillosis can only be achieved by histopathological examination because clinical and radiological findings including MRI are not specific. Surgical intervention and antifungal therapy should be considered the optimal treatment. Early diagnosis and aggressive antifungal treatment provide good results.