• 한국식품저장유통학회지
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• 제17권2호
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• pp.290-296
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• 2010

반추동물의 급성 산독증의 예방 및 치료제 개발을 위해 100종의 브라질 식물 추출물을 이용하여 미생물 유래의 $\alpha$-amylase 및 $\alpha$-glucosidase 저해 활성을 평가하였다. 그 결과 Acacia jurema Mart., Anacardium humile A. St.-Hil., Cedrela odorata L., 및 Guazuma ulmifolia Lam 추출물에서 우수한 $\alpha$-amylase 및 $\alpha$-glucosidase 저해 활성을 확인하였으며, Plumeria drastica Mart.에서는 우수한 $\alpha$-amylase 특이적 저해활성을, Eugenia uniflora L.에서는 강력한 $\alpha$-glucosidase 특이적 저해 활성을 확인하였다. 6종의 추출물을 대상으로 $\alpha$-amylase에 대한 $IC_{50}$ 평가 결과, 현지에서 항당뇨제로 사용되는 A. humile A. St.-Hil., 추출물($IC_{50}$: $91.2{\mu}g/ml$)이 가장 강력한 저해를 나타내었으며, 항당뇨제로 이용되고 있는 acarbose ($IC_{50}$:$50.5{\mu}g/mL$)의 저해활성에 필적하였다. A. humile A. St.-Hil., 추출물은 $80^{\circ}C$, 또는 0.01N HCl 조건에서 60분간 처리에 활성의 변화가 나타나지 않았으며, 산 처리시에는 오히려 활성의 증가가 나타났다. 또한 A. humile A. St.-Hil., 추출물은 인간 적혈구에 대한 용혈활성을 나타내지 않아 반추동물의 급성 산독증 및 인간 당뇨병 치료에도 이용 가능하리라 판단된다.

• 셀메드
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• 제4권1호
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• pp.7.1-7.8
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• 2014

Metabolic effects of ten daily doses of standardized extract of Andrographis paniculata leaves (AP) rich in andrographolide were evaluated in a rat model of type-2 diabetes and in diet induced obese rats. AP was administered per-orally as suspension in 0.3% carboxymethylcellulose at doses of 50, 100 and 200 mg/kg/day for 10 consecutive days. Blood glucose, insulin and lipid profile of rats were measured by using enzyme kits. In addition, effects of such treatments on anti-oxidant enzymes activity and histopathological changes in various organs of diabetic rats were assessed. AP treatments reversed body weight losses and increased plasma insulin level in diabetic rats. The anti-oxidant enzymes activity became normal and histopathological changes observed in pancreas, liver, kidney and spleen of diabetic animals were less severe in extract treated groups. On the other hand, hyperinsulinemia and increased body weight gains observed in high fat or fructose fed rats were less severe in the extract treated groups. These observations revealed therapeutic potentials of the extract for treatments of diabesity associated metabolic disorders, and suggest that the effects of the extract on insulin homeostasis depend on the metabolic status of animals. Activation of cytoprotective mechanisms could be involved in its mode of action.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.90-90
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• 2022

Pueraria flos and Adzuki Beans contains several bioactive compounds, such as saponin, oleanolic acid, and flavone. Pueraria flos and Adzuki Beans has traditionally been used to treat disorders of antioxidant activity, diabetes and liver detoxication, and it has antinociceptive and anti-inflammatory properties. However, complex saponin were validity of the anti-inflammatory activity has not been scientifically investigated. In this study, to determine anti-inflammatory activity of complex saponin separated from Pueraria flos and Adzuki Beans mixture extract on nitric oxide and prostaglandinE₂ assay. The anti-inflammatory activities of complex saponin separated from Pueraria flos and Adzuki Beans mixture extract were evaluated for inhibitory activities against lipopolysacchride induced nitric oxide and prostaglandinE₂ production protein expressions in RAW264.7 cell lines. The complex saponin separated from Pueraria flos and Adzuki Beans mixture extract inhibitory activity for both tests with protein high depressions(%) values showed in the ranges of 50~100 ㎍/ml. Overall, prostaglandinE₂ tests had a higher inhibitory effect on inflammation than nitricoxide tests. Theseis result suggest a potential role of complex saponin separated from Pueraria flos and Adzuki Beans mixture extract as source of anti-inflammation agent.

• 대한본초학회지
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• 제30권2호
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• pp.1-9
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• 2015

Objectives : The present study was conducted to examine whether Toosendan Fructus has an ameliorative effect on diabetes-induced alterations such as oxidative stress and inflammation in the pancreas of non-obese diabetic (NOD) mice, a model of human type I diabetes. Methods : Extracts of Toosendan Fructus (ETF) were administered to NOD mice at three doses (50 mg/kg, 100 mg/kg and 200 mg/kg). Mice at 18 weeks of age were measured glucose tolerance using intraperitoneal glucose tolerance test. After 28 weeks of ETF treatment, glucose, total cholesterol (TC), triglyceride (TG), and proinflammatory cytokines in serum, western blot analyses and a histopathological examination in pancreas tissue, and on the onset of diabetes were investigated. Results : The results showed that levels of glucose, glucose tolerance, TC, TG, interferon-${\gamma}$, interleukin (IL)-1 ${\beta}$, IL-6, and IL-12 in serum were down-regulated, while IL-4, IL-10, SOD, and catalase significantly increased. In addition, ETF improved protein expression of proinflammatory mediaters (such as cyclooxygenase-2, and inducible nitric oxide synthase) and a proapoptotic protein (caspase-3) in the pancreatic tissue. Also, in the groups treated with ETF (100 mg/kg or 200 mg/kg), insulitis and infiltration of granulocytes were alleviated. Conclusions : Based on these results, the anti-diabetic effect of ETF may be due to its anti-inflammatory and antioxidant effect. Our findings support the therapeutic evidence for Toosendan Fructus ameliorating the development of diabetic pancreatic damage via regulating inflammation and apoptosis. Our future studies will be focused on the search for active compounds in these extracts.

• Journal of Acupuncture Research
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• 제25권3호
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• pp.115-125
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• 2008

Diabetes mellitus is a worldwide epidemic. Global projections suggest that most nations will have a doubling of the incidence of Diabetes mellitus(DM) within 20years. The Herb-Combined Remedy(HCR) of DM was known as one of anti-hyperglycaemic agents. But it is unclear whether HCR can be used to treat patients with Diabetic nephropathy or not. The present study was designed to investigate the anti-hyperglycaemic and preventive effects from diabetic nephropathy of HCR in STZ-induced diabetic rats. Treatment with HCR regulated blood glucose levels, but did not affect body weights. In addition, HCR was able to decrease blood glucose levels after 120min. Serum BUN levels were not effective but, serum Creatinine levels were lowered by treatment with HCR or MF. In histopathological observation of renal tissue, both HCR and MF groups showed decreased tendency of inflammation and renal injury. In conclusion, these results demonstrate that HCR is effective to treat patients with DM and also has preventive activity on diabetic nephropathy.

• 디지털융복합연구
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• 제17권7호
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• pp.275-284
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• 2019

난초과의 석곡(石斛; Dendrobii herba) 에탄올 추출물(EED)의 항당뇨 활성 및 항당뇨 기전을 조사하였다. 스트렙토 조 토신에 의해 유도된 당뇨병 마우스에서 EED를 하루 4회 경구 투여하여 공복 혈당, 당화혈색소 수치, 인슐린 수치 및 글루탐산 옥살 아세트산 트랜스 아미나 아제 및 글루탐산 피루브산 트랜스 아미나 아제의 간 기능을 감소시키는 실험연구를 통해 그 약리적 기전을 밝혀내고자 했다. EED는 L6 근육 세포에서 세포 내 포도당 섭취뿐만 아니라 RINm5F 베타 세포에서 포도당에 의한 인슐린 분비를 증가시켰다. 따라서 EED는 인슐린 분비를 증가시킬 뿐만 아니라 세포 당 흡수를 증가시킴으로써 항당뇨병 활성을 나타내는데 커다란 가능성을 보여주었으며, 향후 석곡(石斛; Dendrobii herba) 에탄올 추출물에 대한 약리적 기전에 대한 연구가 더욱 활발하게 이루어져 당뇨병 치료에 큰 공헌을 할 수 있게 되기를 기대해본다.

• Natural Product Sciences
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• 제13권4호
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• pp.311-316
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• 2007

In this study, the twenty-four ethyl acetate extracts of twenty-two medicinal plants, traditionally used in Vietnam as anti-diabetes agents, were investigated for ${\alpha}$-amylase and protein tyrosine phosphatase 1B (PTP1B) enzymes inhibitory activity in vitro. The results indicated that, twelve materials (50.0%) showed moderate to strong inhibitory activity in ${\alpha}$-amylase inhibitory activity with $IC_{50}$ values ranging from 2.5 to $48.8{\mu}g/mL$; meanwhile, ten extracts (41.6%) could demonstrate PTP1B activity with $IC_{50}$ values less than $30.5{\mu}g/mL$. Some plants presented interesting activities against both of ${\alpha}$-amylase and PTP1B enzymes such as Catharanthus roseus, Carthamus tinctorius, Momordica charantia, Gynostemma pentaphyllum, Glycyrrhiza glabra, Smilax glabra, Psidium guajava (leave), and Rehmannia glutinosa. The study may provide a proof, at least in a part, for the ethno-medical use in diabetes disease of these plants.

• Asian Pacific Journal of Cancer Prevention
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• 제17권6호
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• pp.2735-2739
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• 2016

Curcumin, is a polyphenol from Curcuma longa (turmeric plant), is a polyphenol that belongs to the ginger family which has long been used in Ayurveda medicines to treat various diseases such as asthma, anorexia, coughing, hepatic diseases, diabetes, heart diseases, wound healing and Alzheimer's. Various studies have shown that curcumin has anti-infectious, anti-inflammatory, anti-oxidant, hepatoprotective, thrombosuppressive, cardio protective, anti-arthritic, chemo preventive and anti-carcinogenic activities. It may suppress both initiation and progression stages of cancer. Anticancer activity of curcumin is due to negative regulation of inflammatory cytokines, transcription factors, protein kinases, reactive oxygen species (ROS) and oncogenes. This review focuses on the different targets of curcumin to treat cancer.

• The Korean Journal of Physiology and Pharmacology
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• 제8권1호
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• pp.51-55
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• 2004

Sopungsungi-won has been used as a traditional medicine for diabetes and it has been proved to be a potential remedy for type 2 diabetes mellitus. We previously reported that water extract of Sopungsungi-won exhibits anti-diabetic effects both in vivo and in vitro experiments. In the present study, we have chosen to examined anti-apoptotic effect of Rheum undulatum, which is the main component of Sopungsungi-won, on pancreatic ${\beta}-cells$, HIT-T15, against hydrogen peroxide $(H_2O_2)$. oxidative stress. To investigate the anti-apoptotic effect of Rheum undulatum water extract (RUWE) against $H_2O_2-induced$ apoptosis in pancreatic ${\beta}-cell$ line of hamster, HIT-T15, MTT assay, DAPI staining, TUNEL assay, RT-PCR and caspase-3 enzyme assay were performed. The morphological analysis demonstrated that cells treated with $H_2O_2$ exhibited classical apoptotic features, while such changes was reduced in cells pre-treated with RUWE. In addition, RUWE pre-treated cells prior to $H_2O_2$ treatment induced increase of levels of bcl-2 expression and decrease of caspase-3 enzyme activity compared to cells treated with $H_2O_2$ only. These results provide the possibility of usage of RU in patients with progressively deteriorated diabetes.

• 한국식품조리과학회지
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• 제31권2호
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• pp.207-213
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• 2015

구기자의 물추출물 및 50% 에탄올추출물을 이용한 음료의 유리아미노산, 무기질, 산화방지효과, ${\alpha}$-glucosidase 저해활성을 분석하였다. 두 음료에는 6종의 필수 아미노산(isoleucine, leucine, lysine, methionine, threonine, valine)을 포함한 15종의 유리아미노산과 6종의 유도체 등 21종의 아미노산 및 그 유도체가 분석되었다. 물추출물 이용음료와 에탄올추출물 이용음료의 주요 유리 아미노산은 asparagine(115.23, 51.95 mg%), methionine(20.02, 22.07 mg%), aspartic acid (19.65, 13.72 mg%), taurine (18.64, 22.44 mg%)이었으며 무기질은 K(438.80, 426.40 mg%)이 가장 많이 함유되어 있었으며 Mg>Na>Ca>Zn>Fe 순이었다. 산화방지효과는 DPPH 라디칼 소거능과 ABTS 라디탈 소거능으로 측정하였다. 에탄올추출물 이용음료는 물추출물 이용음료에 비해 폴리페놀, 플라보노이드 함량이 높았으나 두 음료의 DPPH 라디칼 소거능과 ABTS 라디칼 소거활성에는 유의적 차이가 없었다. 에탄올추출물 이용음료는 대조군인 acabose에 비해 높은 ${\alpha}$-glucosidase activity 저해활성을 나타내어 혈당저하 효과가 확인되었다. 따라서 50% 에탄올 추출물이 물추출물보다 음료 제조에 적합한 것으로 생각된다.