• 대한한의학방제학회지
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• 제25권3호
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• pp.363-374
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• 2017

Dried fruits of Schizandra chinensis Baillon, Fructus Schisandrae, have been widely used for many years to prevent and treat various diseases in Asian countries including Korea and Russia. It has recently been reported that extracts of Fructus Schisandrae are effective for controlling muscle and skeletal diseases. In this study, we investigated the efficacy of ethanol extract of Fructus Schisandrae (EEFS) on apoptosis and inflammatory response in gastrocnemius muscle of dexamethasone-induced catabolic muscle atrophy mice as part of natural substance discovery and functional analysis for improving muscle function. According to the results of this study, EEFS supplementation attenuated body weight gains and suppressed calf thickness loss in dexamethasone-induced muscle atrophic mice. Gastrocnemius muscle immunohistochemistry showed that expression of caspase-3 and poly(ADP-ribose) polymerase, which are representative apoptotic markers, was markedly increased in dexamethasone control mice; however, their expression was effectively reduced in the EEFS-fed mice. EEFS supplementation also prevented dexamethasone-induced increases in immunoreactivity of muscle fibers for myostatin, an important negative regulator of skeletal muscle mass. In addition, EEFS significantly normalized the increased numbers of nitrotyrosine, 4-hydroxynonenal and inducible nitric oxide synthase-positive muscle fibers compared to that found in dexamethasone control mice. These results suggest that EEFS protects dexamethasone-induced muscular atrophy by decreasing apoptosis and inflammatory responses, and EEFS is more likely to be developed as a muscle strengthening agent.

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.299-307
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• 2021

뇌 내 amyloid beta (Aβ) 축적으로 인한 신경독성은 산화적 스트레스를 야기하여 알츠하이머 질환(Alzheimer's disease, AD)을 유도하는 것으로 알려져 있다. 본 연구는 여주(Momordica charantia L.)의 활성분획물인 butanol (BuOH) 분획물의 Aβ_25-35 유도 AD 동물모델에서 인지능 개선 효과에 대해 연구하였다. T-미로 실험 및 물체인지실험을 통해서 여주 BuOH 분획물 100 및 200 mg/kg/day 농도 투여군은 AD를 유도한 control군에 비해 유의적으로 새로운 경로와 물체를 탐색하는 비율이 감소되어 공간인지 및 물체인지능력 개선 효과를 확인하였다. 수중미로실험을 통해 학습·기억력에 미치는 효과를 측정한 결과, 여주 BuOH 분획물 투여군은 훈련을 반복할수록 숨겨진 도피대를 찾아가는 시간이 감소함을 통해 학습·기억력 개선 효과를 나타내었다. 여주 BuOH 분획물이 산화적 스트레스 개선 효과에 미치는 효과를 확인하기 위해 뇌, 간, 신장 조직에서 지질과 산화 함량 및 nitric oxideNO 생성량을 측정하였다. 여주 BuOH 분획물을 처리한 군은 Aβ_25-35를 주입한 control군에 비해 유의적으로 뇌, 간, 신장 조직에서 지질과산화 함량 및 NO 생성량이 감소되어 산화적 스트레스 개선 효과를 확인하였다. 따라서 본 연구는 여주 BuOH 분획물이 Aβ_25-35 유도 AD 동물모델에서 산화적 스트레스 개선을 통해 인지능력 개선 효과를 나타냄을 확인하였으며, 이에 따라 여주는 AD 예방 및 개선용 소재로써의 가능성이 있는 것으로 사료된다.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.141-146
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• 1999

This study was designed to investigate the effects of silkworm powder (SWP) on oxygen radicals and their scavenger enzymes in serum of rats. Sprague Dawley (SD) male rats (160${\pm}$10g) were fed experimental diets (SWP-200and SWP-400 groups)added 200 and 400mg/kg BW/day for 6 weeks. Triglyceride (TG)levels were significantly inhibited (10% and 25%) in SWP-200 and SWP-400 groups, but there were no singificant differences in total, LDL-and HDL-cholesterol levels in both SWP-200 and SWP-400 groups. Hydroxyl radical ($.$OH) formations resulted in a marked decreases (about 20%) in both SWP-200 and SWP-400 groups compared with control group. Superoxide radical (O2.-)and hydrogen peroxide formations resulted in a significant decreases in SWP-400 group compared with control group. Lipid peroxide (LPO)and oxidized protein(>C=O group) productions resulted in a significant decreases (SOD) and catalase (CAT) activiteies were remarkably increased (10∼25% and 40∼50%)in SWP-200 and SWP-400groups. Glutathione peroxidase(GSHPX) activities were significantly increased (about 10%) in SWP-400 groups compared with control group. These results suggest that anti-aging effect of silkworm powder (SWP) may play a pivotal role in attenuation a various chronic degenerative diseases age-related changes.

• 대한한의학회지
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• 제30권1호
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• pp.51-63
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• 2009

Objectives: Peroxynitrite ($ONOO^-$), superoxide anion radical (${\cdot}{O_2}^-$ and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer's disease, rheumatoid arthritis, cancer, diabetes, obesity and atherosclerosis. The aim of this study was to investigate the $ONOO^-$, NO, ${\cdot}{O_2}^-$ scavenging and NF-${\kappa}B$ related anti-inflammatory activities of Sotosaja-hwan in ob/ob mice. Methods: Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups have received standard chow. The experimental groups were fed with a diet of chow supplemented with 30 and 90 mg Sotosaja-hwan per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho-$I{\kappa}B$-${\alpha}$, anti-IKK-${\alpha}$, anti-NF-${\kappa}B$ (p50, p65), anti-COX-2, anti-iNOS, anti-YCAM-1 and anti-MMP-9 antibodies, respectively. Results: Sotosaja-hwan inhibited the generation of $ONOO^-$, NO and ${\cdot}{O_2}^-$ in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondrial fraction in vitro. The generation of $ONOO^-$, NO, ${\cdot}{O_2}^-$ and PGE2 were inhibited in the Sotosaja-hwan-administered ob/ob mice groups. The GSH/GSSG ratio was decreased in the ob/ob mice, whereas the ratio was improved in the Sotosaja-hwan-administered groups. Sotosaja-hwan inhibited the protein expression levels of phospho-$I{\kappa}B$-${\alpha}$, IKK-${\alpha}$, NF-${\kappa}B$ (p50, p65), COX-2, iNOS, YCAM-1 and MMP-9 genes. Conclusions: These results suggest that Sotosaja-hwan is an effective $ONOO^-$, ${\cdot}{O_2}^-$ and NO scavenger and has NF-kB related anti-inflammatory activity in ob/ob mice. Therefore, Sotosaja-hwan might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.

• 한국의류학회지
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• 제24권4호
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• pp.560-570
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• 2000

The purposes of this study were to segment the cosmeceuticals market based on consumer usage behavior and to develop a profile of each segment using appearance-related variables, purchase attributes, purchase behavior and demographics. A total of 518 responses collected from a questionnaire survey to female consumers was analyzed. Cluster analysis on usage behavior of cosmeceutical products identified three groups including: Anti-aging/Whitening/Slimming product users (22% ); Pore-control product users(20%); and Minimum users(57%). MANOVA, ANOVA and Chi-square analysis revealed significant differences among the three groups on 2 appearance-related variables, 3 purchase attribute factors, 4 purchase behaviors, and 2 demographic characteristics. Based on the results, the study developed a profile of each segment and provided marketing implications.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.863-866
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• 2001

Radish추출물을 수용성과 지용성으로 나누어 추출하여 DPPH 저해율, SOD 활성율, Catalase 활성, collagen 함량변화를 측정한 결과, DPPH 저해율과 Catalase 활성은 수용성 추출물(81.0%, 175units/mg)에서 높게 나타났고, SOD 활성율에서는 지용성 추출물(92.3%)이 높게 나타났다. 콜라겐$(21.45{\mu}g)$내의 pyridinoline의 함량은 control에 비해 수용성과 지용성 추출물을 첨가한 반응액에서 적었으며, 두 추출물을 비교해 봤을 때 큰 차이는 보이지 않았다.

• 한방안이비인후피부과학회지
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• 제27권3호
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• pp.106-114
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• 2014

Objective : UV irradiatiion causes skin-aging involving coarse wrinkles, thickning, dyspigmentation, and rough skin surface. This study was aimed to elucidate the anti-winkle activity of complex diet of Korean red ginseng (RG) and fish oil (FO) on UV-induced Photoaging. Methods : To investigated photo-protective effects of Korean red ginseng and fish oil on UV-induced damaged skins, SKH hairless female mice were randomly divided into six groups [control, UV, UV/RG, UV/FO, UV/RG/FO(low), UV/RG/FO(high)]and orally administered three times a week respectively. UV radiation was applied to the backs of the mice three times a week for 8 weeks. Expressions of matrix metalloproteinase (MMP)-3, MMP-13 and tissue inhibitor matrix metalloproteinase (TIMP)-1 in skin were measured by immunohistochemical staining. Results : In this study, UVB-induced epidermal hypertropy was diminished by RG group or FO group or complex group of RG and FO. Expression levels of MMP-3 and MMP-13 were reduced and expression level of TIMP-1 was increased by RG group or FO group or complex group of RG and FO. Especially MMP-3 and MMP-13 were markedly reduced by diet of FO and complex diet of RG and FO compared with untreated group. Conclusions : This results suggest that complex diet of RG and FO have a anti-wrinkle activity on UV-induced photo-aging and intrinsic aging.

• 생명과학회지
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• 제21권10호
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• pp.1415-1421
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• 2011

이 연구는 L-arginine과 규칙적인 운동이 D-galctose (D-all)투여로 유발된 노화흰쥐의 대동맥에서 발현되는 NF-${\kappa}B$, TNF-a, iNOS, Cav-1, eNOS, Ang II의 변화양상을 관찰하는데 그 목적을 두고 있다. 노화유도 모델 쥐는 D-gal (50 mg/kg)를 숫컷 Strague-Dawley (SD)계 흰쥐의 복강에 1일 1회 총 12주간 투여하여 생산하였으며, 이 실험의 집단은 젊은 대조군(Y-con, n=8), 노화 대조군(A-con, n=8), 노화 운동군(A-Ex, n=8), 노화운동+아르기닌군(A-Ex+A, n=8), 노화 아르기닌군(A-A, n=8)의 5군으로 분류하여 실시하였다. L-arginine은 1일 150 mg/kg씩 총 12주간 경구투여 하였다. 운동방법은 트레이드운동으로 1일 60분씩 20 m/min 속도에서 훈련하였다. 분석결과 1) 유도된 노화군에서 NF-${\kappa}B$, TNF-a, iNOS, Cav-1, 그리고 Ang II 단백질의 발현은 젊은 대조군에 비해 유의하게 증가하였다. 그러나 규칙적인 운동과 L-아르기닌군에서 NF-${\kappa}B$, TNF-a, iNOS, Cav-1, 그리고 Ang II 단백질의 발현은 노화 대조군에 비해 유의하게 감소하였다. 2) 유도된 노화군에서의 eNOS 단백질 발현은 젊은 대조군에 비해 유의하게 감소하였다. 그러나 규칙적인 운동과 L-아르기닌군은 eNOS 단백질의 발현을 더욱 증가시킨 것으로 나타났다. 이상의 결과를 종합하면 12주간 L-아르기닌 투여와 규칙적인 운동 그리고 복합처치는 염증인자와 관련된 단백질인 NF-${\kappa}B$, TNF-a, iNOS 단백질들의 발현을 억제시켜 항염증효과를 보여주었으며, 혈관내피의 기능향상과 관련된 eNOS의 발현을 증가시키는데 긍정적인 역할을 수행할 것으로 기대된다.

• 대한예방한의학회지
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• 제19권1호
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• pp.109-116
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• 2015

Objective : Solar ultraviolet (UV) light can cause photoaging of human skin. Many researchers have focused on effective prevention to mitigate the aging process. This study was aimed to investigate anti-wrinkle effects of pomegranate concentration solution (PCS) using photoaged hairless mouse model. Method : To clarify the protective effect of PCS against UVB-induced photo-damage, water capacity was measured with transepidermal water loss (TEWL) in SKH-1 hairless mice. In addition, image of skin replicas was analyzed. Results : Our results showed that the PCS treatment protects skin against UVB-induced photoaging, based on the parameters including moisture capacity and TEWL. According to visual assessment of skin replica, application of 1 ml/kg PCS inhibited UV-induced wrinkle formation in mice skin as compared to the vehicle-applied control mice. Conclusion : Taken together, these results indicated that PCS could improve skin wrinkle formation induced by UVB irradiation in SHK-1 hairless mice. PCS could be applied as an anti-wrinkle agent.

• 생명과학회지
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• 제22권6호
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• pp.815-822
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• 2012

Resveratrol 유도체의 일종으로 stilbene계열 물질인 piceatannol은 암세포의 증식을 억제하고 apoptosis를 유발하는 것으로 알려져 있다. 본 연구에서는 A549 인체 폐암세포를 대상으로 piceatannol에 의한 암세포 증식억제와 연관된 부가적인 기전연구를 실시하였다. 본 연구의 결과에서 piceatannol이 A549 세포에서 extrinsic 및 intrinsic pathway의 동시 활성을 통하여 apoptosis를 유발하였음을 Fas/FasL의 발현 증가와 caspase-8 및 -9의 활성증가로 확인하였다. 또한 piceatannol은 caspase-3의 활성을 증가시켰으며, caspase-3의 다양한 표적 단백질들의 발현 감소가 동반되었다. 아울러 piceatannol에 의한 apoptosis 유발 과정은 iNOS의 발현 감소에 의한 NO의 생성 억제와도 연관성이 있었다.