• 사상체질의학회지
• /
• 제20권3호
• /
• pp.164-175
• /
• 2008

1. Objective The purpose of this study is to investigate the anti-aging and anti-oxidant effects of Bopyewon-tang(BPW) in Liver and Lung cell. 2. Methods This experiment was used the tissue of liver and lung cells of 6, 52 and 68 weeks old SD rats. Each age group was again divided into three groups. One group, as normal group, was not-treated cells, another group, as control group, was saline-treated cells, and the last group, as experimental group, was BPW -treated cells. After culture for 48 hours, each groups measured the level of SOD, GSH, MDA and NO in the tissue of liver and lung cells. 3. Results and Conclusion 1. The activity of SOD in liver of in 52 w-BPW group, 68 w-BPW group, and in lung of 68 w-BPW were significantly increased in comparison with those of the normal groups. 2. The levels of NO in liver of in 68 w-BPW group was significantly decreased in comparison with those of the normal groups. 3. The levels of MDA in lung of in 68 w-BPW group was significantly decreased in comparison with those of the normal groups.

• 한국수산과학회지
• /
• 제56권4호
• /
• pp.526-531
• /
• 2023

This study investigated the ingredients, anti-aging and anti-dementia activities of the Korean marine algae Silvetia siliquosa. The S. siliquosa solvent extracts were prepared with 70% ethanol, 80% methanol, and distilled water. The extraction yield range of various solvent extracts was 15.82-49.98%. The ethanol and methanol extracts had higher tyrosinase and collagenase inhibitory activities than those of the water extract. Meanwhile, all extracts exhibited high elastase inhibitory activity. Conversely, the methanol and water extracts exhibited the highest acetylcholinesterase inhibitory activity (IC₅₀, 0.40 mg/mL) and β-secretase inhibitory activity (IC₅₀, 0.81 ㎍/mL), respectively. These results indicate that S. siliquosa may be useful in food and pharmaceutical materials as a cosmetic and functional.

• 대한화장품학회지
• /
• 제39권1호
• /
• pp.9-17
• /
• 2013

본 논문에서는 세리신잠 실샘 가수분해물(Sericinjam Gland Hydrolysate: SJGH)을 이용하여 진피 섬유아세포에서 항산화 및 항노화 연구를 진행하였다. SJGH는 사람 섬유아세포에서 고농도의 과산화수소에 의한 세포사멸과 세포 내 산화 증가를 효과적으로 방어하였다. 또한 SJGH는 저농도의 과산화수소에 의한 섬유아세포의 SA-${\beta}$-Gal 발현과 MMP-1의 발현 증가를 억제하였고, 반대로 프로콜라겐 I의 생합성은 증가시켰다. 이러한 결과를 통해 SJGH의 항산화 및 항노화 효과가 우수함을 확인하였으며, SJGH가 항노화 화장품의 우수한 소재가 될 수 있음을 보여준다.

• Biomolecules & Therapeutics
• /
• 제24권3호
• /
• pp.283-290
• /
• 2016

Oroxylum indicum has long been used in Asian traditional medicine to prevent and treat respiratory diseases, diabetes, diarrhea and other conditions. Oroxylin A is a flavone that is present in Oroxylum indicum and in Scutellaria baicalensis. Because the root extracts of both plants have been shown to have anti-allergic effects, the authors investigated whether oroxylin A is likely to have beneficial effects on allergic asthma using female Balb/c mice and rat RBL-2H3 mast cells. Antigen-induced degranulation was measured in vitro by measuring b-hexosaminidase activity. A murine ovalbumin-induced allergic asthma model was used to test the in vivo efficacy of oroxylin A. Sensitization and challenge of ovalbumin induced allergic asthma responses, the accumulations of eosinophils and Th2 cytokine levels in bronchoalveolar lavage fluid and lung tissues. Oroxylin A administration decreased numbers of inflammatory cells, especially eosinophils, and reduced the expression and secretion of Th2 cytokines, including IL-4 and IL-13, in lung tissues and bronchoalveolar lavage fluid. Histologic studies showed oroxylin A reduced inflammatory signs and mucin production in lungs. These findings provide evidence that oroxylin A has potential use as an anti-allergic therapeutic.

• Journal of Applied Biological Chemistry
• /
• 제60권1호
• /
• pp.13-17
• /
• 2017

본 연구는 새로운 기능성 화장품 소재 개발을 위해 복분자 씨앗의 구성성분인 casuarictin을 이용하여 섬유아세포내에서의 항노화 효과를 확인 하고자 하였다. Casuarictin의 항노화 효과를 CCD-986sk 세포를 이용하여 측정한 결과, casuarictin은 UVB에 의해 증가된 ROS 발현을 억제하였으며, pro-collagen의 발현을 증가시키고 MMP-1의 발현을 억제하는 것으로 나타났다. 이는 노화 인자인 활성산소종의 생성 억제와 주름과 밀접한 관련이 있는 콜라겐의 합성 및 분해억제를 통해 항산화 및 주름개선 효과가 있는 것으로 판단되며, 따라서 casuarictin은 화장품 분야에서 항노화 소재로서의 활용가치가 있을 것으로 사료된다.

• 대한화장품학회지
• /
• 제32권2호
• /
• pp.69-74
• /
• 2006

권백은 동양에서 암환자 치료를 위한 전통 약용식물로 알려져 있다. 본 연구에서는 포제기술을 이용한 권백을 항노화 화장품 소재로 적용하고자 다양한 생물학적활성을 평가하였다. 열과 모래를 이용한 포제 권백은 기존 권백을 다른 목적으로 이용하고자 비교하였다. 포제 권백은 페놀함량이 증가하였고 DPPH 라디칼 소거활성도 증가하였다. 세포내 활성산소 소거평가를 위해 사람 섬유아세포를 배양하여 UVB($20 mJ/cm^2$)에 의해 증가된 세포내 활성산소가 포제 권백을 처리함으로써 활성 산소 소거효과가 증가하였다. 사람 섬유아세포에서 UVA에 의해 발현되는 MMP-1효소는 포제 권백에 의해 농도 의존적으로 감소하였다. 결론적으로 포제 권백은 페놀함량의 증가와 항산화 효과가 증가하였고, 자외선에 의한 세포손상을 보호하여 항노화 화장품의 새로운 소재로 이용될 것으로 사료된다.

• 한방안이비인후피부과학회지
• /
• 제34권2호
• /
• pp.1-20
• /
• 2021

Objective : The object of this study was to assess the efficacy of hydrolysate from Phellinus igniarius(HPI) on anti-aging activities in vitro measurement and mini clinical study performed on 5 subjects. Methods : To evaluate skin anti-aging efficacy of HPI, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, type I collagen synthesis, inhibition of nitric oxide(NO) production, inhibiton of tyrosinase, hyaluronan synthase(HAS)2, 3 mRNA expression were measured in vitro. Also, mini clinical study of skin hydration was performed on 5 subjects using HPI in distilled water(DW) and 1,3-butylene glycol diluted solution(30% in DW). Results : 1. DPPH radical scavenging activity of HPI was increased in a dose-dependant. 2. Type I collagen synthesis was increased in 50, 100 and 500㎍/㎖ of HPI. 3. NO production was not inhibited in all concentrations of HPI. 4. Tyrosinase was inhibited in 500, 1000, 2500 and 5000㎍/㎖ of HPI. 5. HAS2 mRNA expression was increased in 50, 100, 150 and 200㎍/㎖ of HPI, HAS3 mRNA expression was increased in 100, 150, and 200㎍/㎖ of HPI. 6. In the mini clinical study of 5 subjects, there was a difference in skin hydration over time for each solutions, but it was not statistically siginificant. Conclusions : HPI increased DPPH radical scavenging activity, type I collagen synthesis, and HAS2, HAS3 mRNA expression. HPI also suppressed tyrosinase. The findings of this study suggest that HPI can be used as an skin anti-aging material.

• 대한본초학회지
• /
• 제34권6호
• /
• pp.109-115
• /
• 2019

Objective : Herbal medicinal mixture (JMB) are consisted of Caryophylli Flos, Aucklandiae Radix, and Angelicae Dahuricae Radix. Each of these herbal medicines has studied on anti-aging effect in vitro. So this study was conducted to investigate efficacy and potency of JMB extract on dermal anti-aging and whitening. Methods : The JMB was extracted at room temperature by 80% ethanol. Collagenase and elastase inhibition activity in JMB ethanol extract were determined at 10, 50, 100, 500, 1000 mg/ml concentrations by colorimetric method. The toxic range of JMB ethanol extract was evaluated using MTT assay. Also, The inhibitory effect of JMB ethanol extract on tyrosinase activity and melanin contents in mouse melanoma cell line (B16F10 cell) was identified at 50, 100, 200 ㎍/㎖ levels by spectrometric assay. In each analysis, EGCG (epigallocatechin gallate) and Kojic acid were used as positive controls, respectively. Results : The elastase and collagenase inhibitory activity of JMB ethanol extract increased dose dependently. Also, The MTT assay showed that JMB, up to 400 ㎍/㎖ concentration, exhibited no toxic effect to the B16F10 cell. And following the JMB ethanol extract treat, cellular melanin contents and tyrosinase activity were dose-dependently decreased compared to those of control. Conclusion : These results suggest that JMB ethanol extract has effects to inhibitory activity on dermal wrinkle enzyme and melanogenesis. Therefore, JMB has applicable benefits for development of materials or products to have whitening and anti-aging functions on skin.

• 한국초지조사료학회지
• /
• 제43권3호
• /
• pp.138-147
• /
• 2023

Buckwheat (Fagopyrum esculentum), which is a traditional Korean crop, has been known as a health food due to its rich nutrition. This study was conducted to evaluate the change in flavonoid content of flowers and seeds during post-flowering growth of Korean tartary buckwheat variety 'Hwanggeummiso', with the aim of providing basic data for the development of functional food and feed additive. Tartary buckwheat took 69 and 99 days from the sowing date to reach the flowering and maturity stages, respectively. As a result of examining the flavonoid components of each part of tartary buckwheat, chlorogenic acid, rutin, and isoquercitrin of flowers increased from the flowering period on 22 May (0 days after flowering) to 42 days after flowering, while quercetin increased until 21 days after flowering and then decreased thereafter. In seeds, chlorogenic acid, rutin, and isoquercitrin were most abundant at the time of seed-bearing on 14 days after flowering, and showed a decreasing tendency thereafter. On the other hand, quercetin showed a tendency to increase until 21 days after flowering and then decrease. Overall, the flavonoid content was higher in flowers than in seeds, with rutin being particularly prominent. Based on this, the possibility as food materials and feed additives was confirmed using buckwheat produced in Korea.

• Journal of Microbiology and Biotechnology
• /
• 제27권2호
• /
• pp.365-371
• /
• 2017

Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanoparticle hydrogen does not disappear easily and collapse under water after long periods of time. We used murine embryonic fibroblasts that were isolated from 12.5-day embryos of C57BL/6 mice. We investigated the ability of nanoparticle hydrogen in water to suppress hydroxyurea-induced ROS production, cytotoxicity, and the accumulation of ${\beta}-galactosidase$ (an indicator of aging), and promote cell proliferation. The accumulation of ${\beta}-galactosidase$ in the cytoplasm and the appearance of abnormal nuclei were inhibited by daily treatment of cells with hydrogen water. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect of hydrogen water was even more remarkable. Thus, this study showed the antioxidant and anti-senescence effects of hydrogen water. Nanoparticle hydrogen water is potentially a potent anti-aging agent.