• Title/Summary/Keyword: antheraea pernyi

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Molecular Cloning and Expression of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi

  • Kim Bo Yeon;Park Nam Sook;Jin Byung Rae;Kang Pil Don;Lee Bong Hee;Seong Su Il;Hwang Jae Sam;Chang Jong Su;Lee Sang Mong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.1
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    • pp.11-17
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    • 2005
  • In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

Complete mitochondrial genome of the Japanese oak silkmoth, Antheraea yamamai (Lepidoptera: Saturniidae), from Jeju Island, Korea

  • Kim, Kee-Young;Park, Jeong Sun;Lee, Keon Hee;Kim, Min Jee;Kim, Seong-Wan;Park, Jong-Woo;Kang, Sang-Kuk;Kim, Nam-Suk;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.44 no.2
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    • pp.65-71
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    • 2022
  • The wild silkmoth Antheraea yamamai Guérin-Méneville, 1861 (Lepidoptera: Saturniidae) is an important producer of silk that is superior to the silk produced by traditional domesticated silkworm. In this study, we sequenced the complete mitochondrial genome (mitogenome) of An. yamamai collected from Jeju Island, which is the southernmost island approximately 100 km offshore southward from the Korean Peninsula. Determining this sequence will be necessary for tracing the biogeographic history of the species and developing molecular markers for identifying the origin of commercial products. Comparison of the sequence divergence among two available and the current mitogenomes revealed a low but substantial number of substitutions, totaling 23 nucleotides in the whole genome. CytB and ND5 showed the highest variability with five and four variations, respectively, suggesting that these regions will be prior regions to target for subsequent biogeographic and diagnosis study. Phylogenetic reconstruction based on all available sequences of Saturniidae showed that An. yamamai is a sister to the congeneric species An. pernyi, corroborating that Antheraea is a highly supported monophyletic group. The tribe Saturniini was clearly non-monophyletic and interrupted by Attacini and Bunaeini.

Molecular Cloning of a cDNA Encoding a Putative Larval and Pupal Cuticle Protein from the Chinese Oak Silkworm Antheraea pernyi

  • Kim, Bo-Youn;Park, Nam-Sook;Jin, Byung-Rae;Lee, Sang-Mong
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.134-135
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    • 2003
  • The insect cuticle undergoes drastic morphological alterations during postembryonic development and is an extracellular structure composed mainly of chitin and proteins that are synthesized and secreted by epidermal cells. Cuticle proteins, the major components of insect integument, are recently being focused as a model fur studying the mechanisms of gene regulation during molting and metamorphosis. (omitted)

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