• 제목/요약/키워드: animal study

검색결과 17,629건 처리시간 0.044초

Genome-wide association analysis of nine reproduction and morphological traits in three goat breeds from Southern China

  • Xiaoyan, Sun;Jing, Jiang;Gaofu, Wang;Peng, Zhou;Jie, Li;Cancan, Chen;Liangjia, Liu;Nianfu, Li;Yuanyou, Xia;Hangxing, Ren
    • Animal Bioscience
    • /
    • 제36권2호
    • /
    • pp.191-199
    • /
    • 2023
  • Objective: This study aimed to investigate the significant single nucleotide polymorphisms (SNPs) and genes associated with nine reproduction and morphological traits in three breed populations of Chinese goats. Methods: The genome-wide association of nine reproduction and morphological traits (litter size, nipple number, wattle, skin color, coat color, black dorsal line, beard, beard length, and hind leg hair) were analyzed in three Chinese native goat breeds (n = 336) using an Illumina Goat SNP50 Beadchip. Results: A total of 17 genome-wide or chromosome-wide significant SNPs associated with one reproduction trait (litter size) and six morphological traits (wattle, coat color, black dorsal line, beard, beard length, and hind leg hair) were identified in three Chinese native goat breeds, and the candidate genes were annotated. The significant SNPs and corresponding putative candidate genes for each trait are as follows: two SNPs located on chromosomes 6 (CSN3) and 24 (TCF4) for litter size trait; two SNPs located on chromosome 9 (KATNA1) and 1 (UBASH3A) for wattle trait; three SNPs located on chromosome 26 (SORCS3), 24 (DYM), and 20 (PDE4D) for coat color trait; two SNPs located on chromosome 18 (TCF25) and 15 (CLMP) for black dorsal line trait; four SNPs located on chromosome 8, 2 (PAX3), 5 (PIK3C2G), and 28 (PLA2G12B and OIT3) for beard trait; one SNP located on chromosome 18 (KCNG4) for beard length trait; three SNPs located on chromosome 17 (GLRB and GRIA2), 28 (PGBD5), and 4 for hind leg hair trait. In contrast, there were no SNPs identified for nipple number and skin color. Conclusion: The significant SNPs or genes identified in this study provided novel insights into the genetic mechanism underlying important reproduction and morphological traits of three local goat breeds in Southern China as well as further potential applications for breeding goats.

돼지 동결 정액을 이용한 체외 수정란 생산 효율 (Effect of Production In Vitro Embryo using Boar Frozen Semen)

  • 조상래;김현종;최창용;손동수;최선호;손준규;김성재;김재범;한만희;진현주
    • 한국수정란이식학회지
    • /
    • 제24권3호
    • /
    • pp.199-205
    • /
    • 2009
  • This study was carried out to investigate the effective genetic resources preservation system using the frozen boar semen. The porcine oocytes were matured for 44 hours in NCSU-23 medium with or without 10% Porcine Follicle Fluid (PFF), 0.5 ${\mu}g/ml$ porcine FSH, 0.5 ${\mu}g/ml$ equine LH, 1.0 ${\mu}g/ml$ 17 $\beta$-estradiol ($E_2$) and 10 ng/ml Epidermal Growth Factor (EGF) under mineral oil at $38.5^{\circ}C$ in humidified atmosphere of 5% $CO_2$ in air. After 44 h of culture, the oocytes were inseminated with frozen-thawed semen and fresh semen prepared with mTBM medium for 6 h. Later, set of 50 presumptive zygotes were transferred into 4-well dish (500 ${\mu}l$) of IVC medium. for embryos freezing, slow-freezing and vitrification methods were used as a cryopreservation. Differences among treatments were analyzed using General Linear Model Procedure by SAS Package (version 6.12) differences were considered significant when p<0.05. Following IVF and IVC, the rates of cleavage and blastocysts formation were significantly higher (p<0.05) in hormone supplemented group than that of hormone-free group (25.7 vs, 12.1). The development rates to cleavage and blastocysts were significantly higher in PZM-5 group than NCSU-23 group (60.3%, 46.6% vs 27.4%, 11.1%). Further improvement was achieved when PZM-5 was supplemented with FBS. Cleavage rates was significantly higher in fresh semen source group than frozen semen (66.7% vs 43.7%). However in blastocysts rates was similar two groups. Post-thaw survival rates of embryos were 1.2% and 2.2% in slow-frezing and vitrification groups, respectively. The results of our study suggest that it is still possible to improve the culture conditions and boar semen cryopreservation for enhance reproductive technology and animal genetic resources conservation.

Genomic Polymorphism Analysis using Microsatellite Markers in Gyeongju Donggyeong Dogs

  • Kim, Seung-Chang;Kim, Lee-Kyung;Choi, Seog-Kyu;Park, Chang-Min;Park, Sun-Ae;Cho, Yong-Min;Lim, Dajeong;Chai, Han-Ha;Lee, Seung-Hwan;Lee, Ji-Woong;Sun, Sang-Soo;Choi, Bong-Hwan
    • Reproductive and Developmental Biology
    • /
    • 제36권4호
    • /
    • pp.243-248
    • /
    • 2012
  • This study was conducted to find a useful marker for gene polymorphism analysis using Microsatellite marker (MS marker) in Gyeongju Donggyeong dog. Twenty three MS marker analyzed the genetic features of DNA using 100 Gyeongju Donggyeong dogs in Gyeongju area. It was performed multiplex PCR with 3 set primer divided 9, 10 and 4 by analysis of conditions among MS markers. The results were calculated heterozygosity, polymorphic information content (PIC), allele frequency and number of allele at each locus using Microsatellite Toolkit software and Cervus 3.0 program. Total 148 alleles were genotyped to determine and average 6.43 alleles was detected. FH3381 had the highest of 15 alleles and FH2834 had the lowest of 2 alleles. Expected heterozygosity had a wide range from 0.282 to 0.876 and had average value of 0.6496. Also, Observed heterozygosity had a more wide range from 0.200 to 0.950 and had average value of 0.6404. PIC had range from 0.262 to 0.859 and average PIC was calculated 0.606. Especially, FH2998 represented the highest rate of observed heterozygosity of 0.950 and FH3381 represented the highest rate of expected heterozygosity of 0.876 and PIC of 0.859. The use of these markers was considered to be useful to study genetic traits of Gyeongju Donggyeong dog.

과배란 처리에 따른 한우와 칡소 체내 수정란 생산 효율과 수정란이식 수태율 (Effect of In Vivo Embryo Production and Pregnancy Rate of Embryo Transfer following Superovulation in Hanwoo and Chickso)

  • 박해금;김남태;김성우;김현;도윤정;염규태;박수봉;김재환;김동훈;조재현;고응규
    • Reproductive and Developmental Biology
    • /
    • 제36권3호
    • /
    • pp.231-235
    • /
    • 2012
  • The objective of this study was to investigate the comparison of transferable embryos and pregnancy rate between Hanwoo and Chickso. The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on Hanwoo 78%, and Chickso 85%, respectively. The mean number of total embryos are each $14.76{\pm}2.16$ and $6.23{\pm}1.07$. So the mean number of transferable embryos are each $10.94{\pm}1.91$ and $4.58{\pm}1.05$. In addition, the mean number of total Hanwoo embryo from <10 and $10{\leq}$ of corpora luteum was $0.50{\pm}0.50$, $11.56{\pm}1.92$, respectively. In case of Chickso, The mean number of transferable embryo from <10 and $10{\leq}$ of CL was $2.75{\pm}1.39$, $6.00{\pm}1.00$, respectively. The pregnancy rates were Hanwoo 40%, and Chickso 37% following transfer of fresh embryos produced in vivo. Also, the pregnancy rates of Chickso 60% were significantly greater (p<0.05) than the Hanwoo 42.48% following transfer of following transfer of frozen embryos, respectively. In conclusion, these results suggest that Chickso may be effectively used for transferable embryos production in Hanwoo. Although the transferable embryos number was not enough, it seems the Chickso greatly affect pregnancy rate. The results indicated that the possibility of transferable embryos from Chickso for embryo transfer could be confirmed in this study. Based on the present findings, it was suggested that it is very important to evaluate in vivo embryo production and pregnancy rate of embryo transfer following superovulation for effective Hanwoo and Chickso production.

Comparative Efficacy of Plant and Animal Protein Sources on the Growth Performance, Nutrient Digestibility, Morphology and Caecal Microbiology of Early-weaned Pigs

  • Yun, J.H.;Kwon, I.K.;Lohakare, J.D.;Choi, J.Y.;Yong, J.S.;Zheng, J.;Cho, W.T.;Chae, B.J.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제18권9호
    • /
    • pp.1285-1293
    • /
    • 2005
  • The present study was conducted to evaluate and compare the effects of various animal and plant protein sources on piglet' performance, digestibility of amino acids and gut morphology in weaned pigs until 28 days after weaning. The plant protein sources used were soybean meal (SBM), fermented soy protein (FSP), rice protein concentrate (RPC); and animal protein sources tested were, whey protein concentrate (WPC) and fishmeal (FM). Iso-proteinous (21%) diets were formulated and lysine (1.55%) content was similar in all the diets. The level of each protein source added was 6% by replacing SBM to the same extent from the control diet containing 15% SBM. The ADG was higher (p<0.05) in the groups fed animal proteins as compared with plant proteins at all the levels of measurement, except during 15-28 days. The highest ADG was noted in WPC and FM fed diets and lowest in SBM fed diet. The feed intake was higher in animal protein fed groups than plant proteins at all phases, but the feed:gain ratio was not affected by protein sources except during overall (0 to 14 day) measurement which was improved (p<0.05) in animal protein fed diets compared to plant protein sources. The digestibilities of gross energy, dry matter and crude protein were higher in animal protein fed groups than for plant protein fed sources. The apparent ileal digestibilities of essential amino acids like Leu, Thr, and Met were significantly (p<0.05) higher in animal proteins fed animals as compared with plant protein fed animals. But the apparent fecal digestibilities of essential amino acids like Arg and Ile were significantly higher (p<0.05) in plant protein diets than animal protein sources. The villous structure studied by scanning electron microscope were prominent, straight finger-like, although shortened and densely located in FM fed group as compared with others. The lactic acid bacteria and C. perfringens counts were higher in caecal contents of pigs fed plant proteins than the animal proteins. Overall, it could be concluded that animal protein sources in the present study showed better effects on growth performance, nutrient digestibility and gut morphology than plant protein sources.

최근 10년간 국내 소 질병 원인체에 관한 문헌적 고찰 (A ten-year retrospective study of bovine infectious disease agents occurred in Korea from 2010 to 2019)

  • 이한규;조아라;오상익;노재희;정영훈;최창용;도윤정;엄재구;손동수;류재규
    • 한국동물위생학회지
    • /
    • 제43권3호
    • /
    • pp.113-128
    • /
    • 2020
  • For estimating the prevalence of bovine infectious disease agents, the pathogens were classified as follows: the digestive disease agents, respiratory disease agents, reproductive disease agents, and tick-borne disease agents. This study covered 81 published papers regarding bovine infectious diseases in Korea that determined the presence of diverse pathogens or the antibodies elicited by the infectious agents in cattle from 2010 to 2019. In total, 59,504 cows were involved in the papers reporting the causative agents in their cases. The disease prevalence for the digestive, respiratory, reproductive, and tick-borne cases was 9.0%, 13.4%, 10.4%, and 7.8%, respectively. Bovine viral diarrhea virus, Escherichia coli, Mycobacterium avium subsp. paratuberculosis, and Eimeria spp were more significantly prevalent in the cows under one-year age than over one-year age. Bovine viral diarrhea virus, Escherichia coli, Mycobacterium avium subsp. paratuberculosis, and Anaplasma spp. were more significantly prevalent in Hanwoo than dairy cattle. Coxiella burnetii, Neospora caninum, and Theilieria spp. were more significantly prevalent in dairy cattle than Hanwoo. Tick-borne disease agents were more prevalent in cows grazing than the case in housing. Our analytic data obtained from this study emphasize the need for more studies on the occurrence of these pathogens according to the breed, age, and the region, to come up with bovine infectious disease control measures in Korea.

국가 종축우에 대한 요네병 항체 양성률 조사 (Seroprevalence of Paratuberculosis in Pure-bred Breeding Cattle in Korea)

  • 김하영;변재원;전병윤;박범수;정지아;박미학;임연수;정병열
    • 생명과학회지
    • /
    • 제22권6호
    • /
    • pp.794-798
    • /
    • 2012
  • 요네병은 Mycobacterium avium subspecies paratuberculosis에 의해 발생하며 반추동물에서 만성 쇠약을 일으켜 전세계적으로 양축산업에 커다란 경제적 손실을 일으키는 질병이다. 본 연구에서는 2008년부터 2009년까지 2년동안 의뢰된 총 3,927건의 국가 종축우의 혈청에 대한 요네병의 항체 양성률에 대해 조사한 결과 21두(0.5%)에서 항체 양성을 나타내었으며 이는 국내 일반 농가의 양성률(1.2-16.4%) 보다 현저히 낮은 수준이었다. 또한 연령별 양성률은 2세 미만에서 0.2% (3두), 2-3세에서 0.4% (2두), 3-4세에서 0.7% (3두), 4세 초과에서 0.9% (13두)로 전 연령에서 양성이 나타났으나 연령이 높아짐에 따라 양성률이 증가하는 경향을 보였다. 품종 별로는 젖소는 모두 음성이었으며 한우에서만 양성률 0.6%를 나타내었다.

한국재래닭 (오계) 원시생식세포에 있어 동결방법의 개선이 융해 후 생존율에 미치는 영향 (The Effect of Modified Cryopreservation Method on Viability of Frozen-thawed Primordial Germ Cell on the Korean Native Chicken (Ogye))

  • 김현;김동훈;한재용;최성복;고응규;도윤정;성환후;김성우
    • Journal of Animal Science and Technology
    • /
    • 제55권5호
    • /
    • pp.427-434
    • /
    • 2013
  • 귀중한 한국재래닭 (오계)의 원시생식세포를 냉동보존하고, 키메라 닭을 통한 재래종의 복원을 도모하는 방법을 실용화하기 위해서, 오계의 원시생식세포에 있어 최적의 동결방법에 대해 검토했다. 원시생식세포의 동결은, 세포를 분리한 후, 동결배지의 기초가 되는 혈청으로서 소 태아혈청 (FBS) 그리고 닭 혈청(CS)를 이용하고, 동결보호제로서는 EG 및 DMSO의 첨가량을 2.5, 5, 10, 15%로 설정하고, 300 ${\mu}L$의 동결배지 용량으로 동결 튜브 안에서 $-70^{\circ}C$로 동결했다. 융해 후에는 오계 PGC의 생존율을 확인 및 검토를 했다. 그 결과, FBS 처리군이 CS 처리군 보다 생존 PGC 회수율이 높은 경향을 나타냈다. 또한 항동해제로서 최적의 조건은 10% EG + FBS의 조합을 기초로 한 동결배지에서 가장 높은 오계원시 생식세포의 생존율을 확인했다. 이러한 결과들로부터 한국재래종(오계)의 원시생식세포의 동결보존의 실용화가 보다 더 향상 될 수 있는 또 하나의 방법이 될 수 있음을 시사한다.

젖소의 생산형질에 대한 305일 보정계수 및 함수식 개발에 관한 연구 (The Studies on The Development of 305-day Adjustment Factors and Formulas for Production Traits in Dairy Cattle)

  • 조광현;이준호;나승환;손삼규;서강석;김시동;최재관
    • Journal of Animal Science and Technology
    • /
    • 제51권2호
    • /
    • pp.111-122
    • /
    • 2009
  • 비유일수별로 변화하는 유생산기록을 305일로 보정하기 위하여, 분만 시 나이와 분만계절, 산차를 고려한 305일 보정계수를 개발하였고, 비선형적 특성에 적합하고 사용을 용이하게 하는 거듭제곱 함수 형태의 보정 공식을 개발하였다. 각각의 보정계수가 얼마나 정확하게 기록을 보정 할 수 있는 지 측정하기 위하여 실제 305일 누적 착유량 및 개체별 누적 착유량 평균과의 오차를 비교 분석 한 결과 새롭게 개발된 보정계수의 오차가 가장 적게 나타났으며, 이를 토대로 한 보정공식 역시 작은 오차를 나타냈다. 유생산량의 평균치가 최근까지 많이 증가하였음에도 불구하고 2산 이상의 개체에서 2002년에 개발된 보정계수의 오차가 낮게 나타난 것으로 보아 앞으로의 보정계수 개발에는 2002년 보정계수 개발에 사용되었던 건유효과에 대한 부분을 추가해야 더 정확하게 보정할 수 있을 것으로 사료된다. 유량에서의 보정계수 개발 및 보정식 개발 방법으로 유지방, 유단백, 무지고형분량에 대해서도 비유 일수 305일째의 누적량으로 보정할 수 있는 보정 공식을 개발하였다.

Comparative study of nutritional composition and color traits of meats obtained from the horses and Korean native black pigs raised in Jeju Island

  • Seong, Pil-Nam;Kang, Geun-Ho;Cho, Soo-Huyn;Park, Beom-Young;Park, Nam-Geon;Kim, Jin-Hyoung;Ba, Hoa Van
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제32권2호
    • /
    • pp.249-256
    • /
    • 2019
  • Objective: The present study aimed at comparing the nutritional composition and color traits between two meat types: Horse meat and pork from Korean native black pigs raised in Jeju Island. Methods: After slaughter 24 h, the longissimus dorsi samples were taken from left side carcasses of the 32-mo-old Jeju female breed horses and the 6-mo-old Korean native black pigs (n = 10 each). The samples were then placed into cool boxes containing ice packs and transported to the Laboratory of Meat Science where all visual fats and connective tissues were trimmed off and then the samples were ground. All the samples were analyzed for nutritional composition (proximate composition, minerals, vitamins, fatty acids, and amino acids) and color traits. Results: The horse meat contained significantly higher collagen, moisture and protein than the pork (p<0.05). The Jeju horse meat showed more desirable fatty acid profiles such as containing significantly lower saturated fatty acids (SFA), higher polyunsaturated fatty acids (PUFA) contents and PUFA/SFA ratios than the pork (p<0.05). Differences in concentrations of ten amino acids existed between the two meat types in which the horse meat had higher values for all these amino acids, total amino acids (20.33 g/100 g) and essential amino acids (10.06 g/100 g) than the pork (p<0.05). Also, the horse meat showed significantly higher concentrations of Fe (34.21 mg/100 g) and Cu (2.47 mg/100 g) than the pork (Fe, 17.42 mg/100 g and Cu, 1.51 mg/100 g) (p<0.05). All the vitamins detected showed statistical differences between the two meat types in which the horse meat had higher concentrations of vitamin B1 (25.19 mg/100 g), B2 (92.32 mg/100 g), B3 (2,115.51 mg/100 g), and B5 (67.13 mg/100 g) than the pork (p<0.05). Conclusion: Based on the results obtained in the study, it is concluded that the two meat types studied are rich in nutrients and the animal species strongly affected the nutritional values and color traits of the muscle tissues.