• Journal of Korean Biological Nursing Science
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• 제17권4호
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• pp.324-330
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• 2015

Purpose: The purpose of this study was to analyze the trends in Korea of nursing research using animals. Methods: 45 articles were reviewed with descriptive statistics from 2000 to 2015. Results: Nearly 50% of the studies were supported by research funds. Most of the animal species in the studies were rats (81.1%) and mice (15.6%). The major animal models for human disease were cerebrovascular diseases (24.4%), skin problems (17.8%), and nerve injuries (15.5%). Immunohistochemistry (24.3%) was mainly used as an experimental technique. The main intervention and treatments used in the studies were chemical treatments, exercise and surgery. Conclusion: In this review, relevant results were described to identify the trends of nursing research using animal experimentation in Korea. Expansion of various nursing research using animal models can be required for gaining knowledge applicable to the same conditions in humans.

• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.485-493
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• 2019

Objective: This study was undertaken to investigate the genetic characteristics of Berkshire (BS), Landrace (LR), and Yorkshire (YS) pig breeds raised in the Great Grandparents pig farms using the single nucleotide polymorphisms (SNP) information. Methods: A total of 25,921 common SNP genotype markers in three pig breeds were used to estimate the expected heterozygosity ($H_E$), polymorphism information content, F-statistics ($F_{ST}$), linkage disequilibrium (LD) and effective population size ($N_e$). Results: The chromosome-wise distribution of $F_{ST}$ in BS, LR, and YS populations were within the range of 0-0.36, and the average $F_{ST}$ value was estimated to be $0.07{\pm}0.06$. This result indicated some level of genetic segregation. An average LD ($r^2$) for the BS, LR, and YS breeds was estimated to be approximately 0.41. This study also found an average $N_e$ of 19.9 (BS), 31.4 (LR), and 34.1 (YS) over the last 5th generations. The effective population size for the BS, LR, and YS breeds decreased at a consistent rate from 50th to 10th generations ago. With a relatively faster $N_e$ decline rate in the past 10th generations, there exists possible evidence for intensive selection practices in pigs in the recent past. Conclusion: To develop customized chips for the genomic selection of various breeds, it is important to select and utilize SNP based on the genetic characteristics of each breed. Since the improvement efficiency of breed pigs increases sharply by the population size, it is important to increase test units for the improvement and it is desirable to establish the pig improvement network system to expand the unit of breed pig improvement through the genetic connection among breed pig farms.

• 응용통계연구
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• 제25권1호
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• pp.115-123
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• 2012

In animal tumorigenicity data, tumor onsets occur at several sites and onset times cannot be exactly observed. Instead, the existence of tumors is examined only at death time or sacrifice time of the animal. Such an incomplete data structure makes it difficult to investigate the effect of treatment on tumor onset times; in addition, such dependence should be considered when censoring due to death is related with tumor onset. A bivariate frailty effect is incorporated to model bivariate tumor onsets and to connect death with tumor. For the inference of parameters, EM algorithm is applied and a real NTP(National Toxicology Program) dataset is analyzed as an illustrative example.

• Journal of Animal Science and Technology
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• 제52권6호
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• pp.459-466
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• 2010

In this study, we suggest an objective standard in selection of candidate horse mates. Korea Racing Authority provided racing records and pedigree information of 44 sires and 954 dams. The datasets were used to predict Racing Indices represented by the averages of earnings earned by offspring for each dam and sire that indicate the racing performance of its domestic progeny. Proportion of wins and second places to the number of taken races and the mean of distances for the won races of a sire were significant factors in linear model with minimum prediction errors. For dam, those factors were the average of earned money per race, number of outstanding broodmares in pedigree, and the comparable index which indicates the relative affinity with its mate. We can use the resultant model for a horse mate by choosing one of the candidates with the largest predicted value for hypothetical offspring.

• Asian-Australasian Journal of Animal Sciences
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• 제24권11호
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• pp.1504-1513
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• 2011

It is important to identify genetic interactions related to human diseases or animal traits. Many linear statistical models have been reported but they did not consider genetic interactions. Genotype matrix mapping (GMM) has been developed to identify genetic interactions. This study uses the GMM method to detect superior SNP combinations of the CCDC158 gene that influences average daily gain, marbling score, cold carcass weight and longissimus muscle dorsi area traits in Hanwoo. We evaluated the statistical significance of the major SNP combinations selected by implementing the permutation test of the F-measure. The effect of g.34425+102 A>T (AA), g.8778G>A (GG) and g.4102+36T>G (GT) SNP combinations produced higher performance of average daily gain, marbling score, cold carcass weight and the longissimus muscle dorsi area traits than the effect of a single SNP. GMM is a fast and reliable method for multiple SNP analysis with potential application in marker-assisted selection. GMM may prospectively be used for genetic assessment of quantitative traits after further development.

• 한국통계학회:학술대회논문집
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• 한국통계학회 2003년도 춘계 학술발표회 논문집
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• pp.305-311
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• 2003

K-Means 모델을 이용하여 한우 유전자 6번의 BM4311의 중요 DNA marker을 찾기위해 여러 가지로 시도해 왔다. 이번 논문에선 QTL(Quantitative Trait Loci)과 data mining modeling를 이용하여 BM4311에서 중요 DNA marker를 찾아 보도록 하겠다.

• 농업과학연구
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• 제38권1호
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• pp.45-50
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• 2011

This study was conducted to investigate the farm situation about bovine leukemia virus(BLV) infection that greatly influence productivity in dairy cattle and compare the accuracy of diagnosis for BLV infection between PCR and ELISA techniques. Blood samples of 193 heads from 5 herds in Chungnam and Chungbuk area were used to analyze BLV gene and serum, and the results were obtained as follows. The amplified BLV gene in dairy cattle by PCR technique resulted in 226 bp, 596 bp and 434 bp, respectively, for gag, pol and env, which were well amplified. The infection rates of BLV virus diagnosed by PCR and ELISA techniques ranged from 80.55 to 100% and from 22.22 to 86.95%, respectively, and the infection rates among 5 herds were significantly different in both methods (P<0.05). Further, the average infection rates of 5 herds were 87.05 and 63.21%, respectively, for PCR and ELISA techniques. Kappa statistics for examining consistency of diagnosis by PCR and ELISA techniques showed 0.246, which represents low consistency. Consequently, PCR based BLV technique was considered as a corrective measure for diagnosis of BLV infection in Holstein dairy cattle.

• Journal of the Korean Data and Information Science Society
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• 제13권2호
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• pp.97-104
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• 2002

K-Means modelling has been tried for finding major DNA marker of ILST035 microsatellite loci in Hanwoo Chromosome 6 linkage map. Major DNA markers are obtained from the ILST035 microsatellite through quantitative trait loci(QTL) and data mining modelling.

• 한국농림기상학회지
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• 제17권3호
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• pp.261-269
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• 2015

본 연구는 탐색적 자료분석을 이용하여 사일리지용 옥수수(whole crop maize, WCM) 연도별 건물수량 변동의 유의성과 이와 관련 있는 기후요인의 유의성을 확인 하는데 목적이 있다. WCM의 원자료는 30년간의 농협중앙회 수입적응시험 심의결과(7개도 124품종 1,027점)였으며, 이 중 미비하거나 중복된 자료는 삭제 또는 수정하여 최종적으로 7개 도에서 124품종 993점을 이용하였다. WCM의 상대숙도와 출사일수를 기준으로 분류하면 조생종(25품종 200점), 중생종(40품종 409점), 만생종(27품종 234점)및 기타(32품종 150점)였다. 기상자료를 이용하여 기후요인을 측정하기 위한 6개의 기상변수를 생성하였다. 건물수량 및 기후요인을 탐색하기 위해서 기술통계량 및 정규성검정을 실시하였으며, 통계분석은 SPSS 21.0을 이용하였다. 연도별 평균 건물수량을 상위와 하위집단으로 분류하여 비교한 결과 상위와 하위집단 간에는 건물수량의 유의적 차이가 있었다(p<0.05). 두 집단간 차이의 원인을 구명하기 위하여 기후관련 요인들을 분석한 결과 파종수확적산생육온도일수(파종~수확 생육온도일수의 합), 파종수확일조시간(파종일부터 수확일까지의 일조시간의 합) 및 파종수확강수량(파종일부터 수확일까지 일강수량의 합)에서 집단간 유의적 차이가 나타났다(p<0.05). 반면 파종수확강수일수(파종에서 수확까지 강수일수)는 건물수량에 영향을 미치지 않았다(p>0.05). 이상의 결과에서 여러 기상변수 중 파종에서 수확까지의 적산온도, 일조시간 및 강수량이 WCM의 건물수량과 연관이 있는 것으로 나타났다. 그러나 세 변수 간의 기여도($R^2$)는 비교할 수가 없어 추후 회귀분석을 이용하여 WCM의 건물수량에 미치는 각 변수의 기여도 및 건물수량 예측모형을 구축할 필요가 있다.

• Journal of the Korean Data and Information Science Society
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• 제17권4호
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• pp.1041-1051
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• 2006

The effectiveness of genetic markers on Hanwoo traceability systems was applied and evaluated from Korean 33 Hanwoo elite sire families. Five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Eleven major microsatellite markers were selected from allele amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification with experimental condition, the match probability(MP) and the relatedness coefficient(R) were computed.