• Journal of Nutrition and Health
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• v.1 no.2
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• pp.61-85
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• 1968

The experiment was carried out on eight healthy male subjects of 19-22 years old and they were maintained The experiment was carried out on eight healthy male subjects of 19-22 years old and they were maintained under the rice diet, the general mixed diet and the high animal protein diet for 7 days of each experimental period, respectively. The composition of each diet was indicated in Table 3. The allowance' of each nutrient per head per day in each diet were as follows. a) In the rice diet; protein:139.5 g (animal source:64.6 g, vegetable source:74.9 g), fat:25.3 g (animal source:11.3 g, vegetable source:14.0 g), carbohydrate: 644.0 g, calcium:982.3 mg, phosphorus: 2369.2mg, thiamine:2.1 mg, riboflavin: 1.6 mg, and 3211 Cal. b) In the general mixed diet; protein: 97.4g (animal source: 10.5 g, vegetable source: 86.9 g), fat:40.3 g (animal source:7.1 g, vegetable source:33.2 g), carbohydrate:620.7 g, calcium:887.1m g, phosphorus:2200.8m g, thiamine: 1.8m g, riboflavin:1.9m g, and 3158 Cal. c) In the high animal protein diet; protein: 135.6 g (animal source:68.8 g, vegetable source:66.8 g), fat:32.5g (animal source:17.9 g, vegetable source:14.6 g), carbohydrate:930.9 g, calcium: 626.0 mg, phosphorus: 1998.9 mg, thiamine: 1.5 mg, riboflavin: 1.5 mg, and 3194 Cal. The absorption rates of protein, fat, carbohydrate, calcium, phospherus, thiamine and riboflavin in each diet were observed. The results obtained were summarized as follows: 1. The absorption rates of protein and fat in the rice diet were 88.t % and 71.3.%. 2. The absorption rates of protein and fat in the general mixed diet were 83.4% and 86.4%. 3. The absorption rates of protein and fat in the high animal protein diet were 86.8% and 82.4%. 4. The nitrogen balances in the rice, the general mixed and the high animal protein diet groups were +5.7g, +2.3g and +4.0g respectively. 5. The absorption rates of carbohydrate in each diet were all above 95 % and so seemed to be almost completely absorbed except the cellulose in the diets. 6. The calory utilization rates in each diet were all above 93 %. 7. The minimum absorption rates of protein, fat and calory were 83.8%, 63.3%, and 89.7% in the rice diet, 80.0%, 80.9% and 85.9%, in the general mixed diet and 83.6%, 75.7% and. 89.3 % in the high animal protein diet respectively. Therefore, it is assumed that these data might be significant in practical use. 8. The protein absorption rates of the rice diet (88.1 %) was better than that of the general mixed diet (83.4 %). 9. The fat absorption rates of the general mixed diet and the high animal protein diet (86.4% and 82.4%) were signficantly better than that of the rice diet (71.3%). 10. The calcium absorption rates of each diet, rice diet, the general mixed diet and the high animal protein diet were 41.6%, 36.0%, and 27.7%, respectively. 11. The phosphorus absorption rates of each diet were 51.4%, 56.0% and 52.3%, respectively.12. The phosphorus absorption rate seemed better than that of calcium. 13. The thiamine absorption rates of each diet seemed 27.0 %, 42.9 % and 29.5 %, respectively. 14. The riboflavin absorption rates of each diet seemed above 30.6%, 27.1%, and 39.3% respectively. 15. The excretion amounts of thiamine or riboflavin were much more than the amounts ingested of the both vitamins. Therefore, the certain amount of both vitamins seemed to synthesize in the intestine.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1593-1599
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• 2014

This study consisted of two experiments, conducted to assess the impact of phase at which vegetable protein (VP) diets are introduced to broiler chicks, and preference of birds for diets based on soybean or canola meal (CM). Two hundred and ten day-old Cobb 500 chicks were randomly distributed into five dietary groups in the main experiment. One group was fed on animal protein (AP) diet all through to 21 days of age; two other groups were started on AP diet for 7 days and then switched to diets containing soybean meal (AP-SBM) or AP-CM, while two other diets (SBM-AP and CM-AP) were started on one of the VP diets for 7 days and then switched to AP diet. A sub-experiment on thirty birds raised on a commercial diet to 7 days was used in a feed selection test to quantify the preference of birds for the diets containing mainly CM or SBM. Chicks were reared under similar care and management conditions and the diets were iso-caloric and iso-nitrogenous. Results of the main experiment showed that chicks on CM-AP diet ate more (p<0.05) than those on the other diets up to day 7. Body weight gain was highest (p<0.001) on the AP-SBM diet while birds on the CM-AP diet weighed the least at 7 d. Feed intake, body weight gain, feed conversion ratio, mortality, bone growth, visceral organ development, and activities of digestive enzymes were similar between the groups from hatch to 21 days of age. Results of the second sub-experiment showed that chicks preferred the CM-based diets to the SBM-based diets at 8 to 14 d (p<0.001) and 15 to 21 d (p<0.01) when given a choice. Overall, the birds were not affected by the nature of the starter diet although they tended to prefer the canola to soybean diets.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.3
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• pp.387-394
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• 2019

Objective: This study was to investigate the nutrient ileal digestibility of dried mealworm (Tenebrio molitor) larvae and compare with those of three animal protein by-products in growing pigs. Methods: A total of 12 crossbred ($[Landrace{\times}Yorkshire]{\times}Duroc$) growing pigs with average body weights of $24.12{\pm}0.68kg$ were surgically equipped with simple T-cannulas after being deprived of feed for 24 h according to published surgical procedures. These pigs had a recovery period of two weeks. A total of 12 pigs were assigned to individual metabolic crates and allotted to one of four treatments with 3 replicates in a fully randomized design. Dietary treatments included the following: i) Fish meal, corn-vegetable by-product basal diet+9.95% fish meal; ii) Meat meal, corn-vegetable by-product basal diet+9.95% meat meal; iii) Poultry meal, cornvegetable by-product basal diet+9.95% poultry meal; iv) Tenebrio molitor, corn-vegetable by-product basal diet+9.95% dried Tenebrio molitor larvae. Results: Results showed that the apparent ileal digestibility (AID) of Lys was higher (p<0.05) in pigs fed Tenebrio molitor diet than that in pigs fed fish meal diet. Pigs fed Tenebrio molitor diet showed increased (p<0.05) AID of His and Arg compared to pigs fed Fish meal or Meat meal diet. The AID of Cys was increased (p<0.05) in pigs fed poultry meal and Tenebrio molitor diets compared to that in pigs fish meal diet. Pigs fed meat meal, poultry meal, and Tenebrio molitor diets showed higher (p<0.05) standardized ileal digestibility (SID) of total energy compared to pigs fed fish meal diet. The SID of Arg was higher (p<0.05) in pigs fed Tenebrio molitor diet than that in pigs fed fish meal or meat meal diet. Furthermore, pigs fed poultry meal or Tenebrio molitor diets showed increased (p<0.05) SID of Cys compared to pigs fed fish meal diet. Conclusion: In conclusion, providing pigs with diets that contained Tenebrio molitor larvae meal improved AID and SID of nutrients as well as essential and non-essential amino acids. The digestibility of dried mealworm larvae protein and its utilization in vivo are also good. Therefore, dried mealworm larvae protein can be used as protein source at 10% level in growing pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.6
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• pp.825-832
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• 2010

The United Nations Convention on Biological Diversity defines biotechnology as "Any technological application that uses biological systems, dead organisms, or derivatives thereof, to make or modify products or processes for specific use" Biotechnology has made tremendous contributions to improve production efficiency of agriculture during the last century. This article reviews successful examples of application of bio-fermentation in improving swine nutrition efficiency mainly based on the authors'z own research experience. Production of feed grade supplemental amino acids by bio-fermentation allowed nutritionists to formulate accurate feed for optimal lean growth and reduced nitrogen excretion. Recent issues with high feed grain prices caused potential feed quality problems. Bio-fermentation allowed nutritionists to use exogenous supplemental enzymes such as phytase and NSPases in swine diets, thereby improving nutrient utilization and reducing nutrient excretion to the environment. Yeast metabolites are also produced by bio-fermentation and have been repeatedly shown to improve milk production of sows during early lactation even though actual mechanisms are still to be investigated. Bio-fermentation technology also allowed nutritionists to prepare vegetable protein sources with large protein molecules and anti-nutritional factors suitable for feeding newly weaned piglets, as selected microorganisms significantly reduce specific anti-nutritional factors and size of peptides. Preparations of vegetable protein sources suitable for newly weaned pigs will greatly contribute to swine nutrition by providing efficient alternatives to the use of animal protein sources that are often expensive and somewhat against societal preference. Considering the few examples listed above, biotechnology has closely influenced improvement of production efficiency in the swine industry. As we have limited resources to produce meat to satisfy ever-increasing global demands, extensive adaptation of biotechnology to enhance production efficiency should be continued. However, at the same time, wise and careful application of bio-technology should be considered to ensure production of safe food and to meet the expectations of our society.

• Food Science of Animal Resources
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• v.44 no.5
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• pp.1028-1039
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• 2024

Alternative protein sources with greater nutritional value and a lower environmental footprint have recently attracted interest in the production of meat substitutes. However, it is required that these alternatives mimic the texture and structure of meat. This study investigated varying ratios of textured vegetable proteins (TVP) to Tenebrio molitor larvae (brown mealworm; TM) with the addition of transglutaminase (TG) to determine the quality characteristics of these emulsions. The results demonstrated low protein solubility of the emulsions as TVP content increased. Furthermore, when the proportion of TM was high, the TG-treated emulsion had a low pH. Additionally, when there was a high TM ratio to TVP in the TG treatment, the emulsions demonstrated better thermal stability and water holding capacity. Regarding the rheological properties of the emulsion, both the frequency-dependent storage modulus (G') and loss modulus (G'') increased as the proportion of TVP in the emulsion increased with and without the addition of TG. Differential scanning calorimetry analyses demonstrated two protein denaturation peaks in all treatments, with high peak temperatures for both treatments with a high proportion of TM. The hardness and chewiness of the emulsion were highest in the treatment (T6 and T8) with TG, and the gumminess of the emulsion was greatest when TM only or when equal ratios of TVP and TM were treated with TG, respectively. In conclusion, the addition of TM to TVP with TG improves the overall texture of the protein mixture, making it a suitable meat alternative.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.9
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• pp.1255-1262
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• 2000

A total of 80 pigs ($5.68{\pm}0.61kg$ BW; 21 d of age) were used to evaluate the effect of fat sources in weaned pigs. Pigs were allotted into five treatments based on body weight, in a completely randomized block design. Treatments consisted of diets representing different ratio between soy oil or corn oil and tallow; 1) S100 (soy oil 100%), 2) S75 (soy oil:tallow=75:25), 3) S50 (soy oil:tallow=50:50), 4) C75 (corn oil:tallow=75:25), 5) C50 (corn oil:tallow=50:50). During d 0 to 14, pigs were fed diets containing 3,400 kcal ME, 23% crude protein, 1.65 % lysine and for the period of d 15 to 28, pigs were fed diets supplying 3,400 kcal ME, 20.5% crude protein, 1.355% lysine. For d 0 to 14, a significant differences in ADG and ADFI were observed among treatments, pigs fed animal-vegetable fat blends gained more than pigs fed the S100. As tallow addition was increased from 25% to 50% replacing soy or corn oil, ADG was improved from 6.32% to 28.38%. In phase II (d 15 to 28) period, ADG, ADFI and FCR were not significantly different among treatments. For overall period (d 0 to 28), pigs fed 50% animal:50% vegetable fat blends diets consumed more feed and grew faster than pigs fed control diet and 75% vegetable oil:25% tallow groups, but the differences was not significant among treatments. Apparent DM, CP and fat digestibility were increased with time postweaning for each treatment. There were no differences in digestibilities of total amino acid during overall period. The combination of soy oil or corn oil with tallow produced slightly higher serum triglycerides (TG) and total cholesterol (TC) combination compared with the soy oil alone. In conclusion, tallow:soy (or corn) oil blends diets improved growth rate and the ratio of 50% tallow plus 50% soy (or corn) oil blends diets were favorable for growth and nutrient digestibilities of weaned pigs.

• Food Science of Animal Resources
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• v.30 no.5
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• pp.730-738
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• 2010

We developed predictive growth models for Staphylococcus aureus and Bacillus cereus on various food matrices consisting primarily of ready-to-eat (RTE) foods. A cocktail of three S. aureus strains, producing enterotoxins A, C, and D, or a B. cereus strain, were inoculated on sliced bread, cooked rice, boiled Chinese noodles, boiled bean sprouts, tofu, baked fish, smoked chicken, and baked hamburger patties at an initial concentration of 3 log CFU/g and stored at 8, 10, 13, 17, 24, and $30^{\circ}C$. Growth kinetic parameters were determined by the Gompertz equation. The square-root and Davey models were used to determine specific growth rate and lag time values, respectively, as a function of temperature. Model performance was evaluated based on bias and accuracy factors. S. aureus and B. cereus growth were most delayed on sliced bread. Overall, S. aureus growth was significantly (p<0.05) more rapid on animal protein foods than carbohydrate-based foods and vegetable protein foods. The fastest growth of S. aureus was observed on smoked chicken. B. cereus growth was not observed at 8 and $10^{\circ}C$. B. cereus growth was significantly (p<0.05) more rapid on vegetable protein foods than on carbohydrate-based foods. The secondary models developed in this study showed suitable performance for predicting the growth of S. aureus and B. cereus on various food matrices consisting of RTE foods.

• Food Science of Animal Resources
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• v.42 no.2
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• pp.295-312
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• 2022

Inulin is a non-digestible carbohydrate and a prebiotic that can also act as a fat replacer in various foods. This study examined the effect of replacing vegetable oil with garlic inulin on the quality traits of chicken sausages. Water-based inulin gels were prepared using garlic inulin or commercial inulin to imitate fats in chicken sausages. Chicken sausages were prepared separately replacing vegetable oil with water-based inulin gels to reach final inulin percentages of 1, 2, and 3 (w/w). The control was prepared using 3% (w/w) vegetable oil with no inulin. The physicochemical properties and thiobarbituric acid reactive substance (TBARS) value of prepared sausages were analyzed over 28-d frozen storage. Sausages with 2% garlic inulin recorded higher flavour and overall acceptability scores (p<0.05). Ash, moisture, and protein contents of the sausages were increased with increasing levels of inulin while fat content was reduced from 13.67% (control) to 4.47%-4.85% (p<0.05) in 3% inulin-incorporated products. Sausages incorporated with 2% inulin had lower lightness (L^*) values than the control (p<0.05). Water holding capacity (WHC) was similar (p>0.05) among the samples. During storage L^* value, pH, and WHC decreased while redness (a^*) and yellowness (b^*) values increased in all the samples. In addition, TBARS values were increased during the storage in all samples within the acceptable limits. In conclusion, garlic inulin can be used successfully as a fat substitute in sausages without altering meat quality parameters.

• Food Science of Animal Resources
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• v.35 no.2
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• pp.189-196
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• 2015

The aim of this study was to manufacture functional high protein fermented beverage, using whey protein concentrate (WPC) and Lactobacillus plantarum DK211 isolated from kimchi, and to evaluate the physicochemical, functional, and sensory properties of the resulting product. The fermented whey beverage (FWB) was formulated with whey protein concentrate 80 (WPC 80), skim milk powder, and sucrose; and fermented with Lactobacillus plantarum DK211 as single, or mixed with Lactococcus lactis R704, a commercial starter culture. The pH, titratable acidity, and viable cell counts during fermentation and storage were evaluated. It was found that the mixed culture showed faster acid development than the single culture. The resulting FWB had high protein (9%) and low fat content (0.2%). Increased viscosity, and antioxidant and antimicrobial activity were observed after fermentation. A viable cell count of 10⁹ CFU/mL in FWB was achieved within 10 h fermentation, and it remained throughout storage at 15℃ for 28 d. Sensory analysis was also conducted, and compared to that of a commercial protein drink. The sensory scores of FWB were similar to those of the commercial protein drink in most attributes, except sourness. The sourness was highly related with the high lactic acid content produced during fermentation. The results showed that WPC and vegetable origin lactic acid bacteria isolated from kimchi might be used for the development of a high protein fermented beverage, with improved functionality and organoleptic properties.

• Journal of the Korean Society of Food Science and Nutrition
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• v.9 no.1
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• pp.39-44
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• 1980

The effect of dietary vegetable oils on the growth and blood glucose level of rabbit was examed. This study was carried out to observe the nutritive effect of feeding vegetable oils for a period of weeks. The experimental diets were prepared on the basis of isocaloric and isonitrogenous diet. The Experimental animals were fed on 5 kinds of diets such as control (Basal), group A(Basal + sesame oil), group B(Basal + perilla oil), group C(Basal + soybean oil), and group D(Basal + rice bran on diets for 4 weeks. The results obtained are summarized as follows: 1. The gain in body weight of rabbit was higher for perilla oil fed group than any other group gained. 2. Liver weight of the control group, group A, group B, group C, and group D were 30.35 or 37.35 or, 37.25 or, 38.25 gr, 31.05 gr, and 39.54 gr, respectively. The reletionship between liver and body weight were 3.25, 3.99, 4.33, 4,15, and 4.57, respectively. It showed that to liver weight of group D which was fed rice bran oil was heavier than any other group showed. 3. The content of total protein and glucase level in serum of animal blood were 5.72 mg% 40.34 mg%, 5.65 mg%: 22.37 mg%, 5.95 mg%: 77.0 mg%. 5.62 mg%: 28.60 mg%, and 5.63 mg%: 34.10 mg%, respectively. As shown above, the group B was the most heaviest one. 4. It may be concluded from the above results that linolenic acid which was included in vegetable oils have an effect on interconversion among three caloric elements such as cabohydrate, protein, and fats.