• Natural Product Sciences
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• v.14 no.1
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• pp.56-61
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• 2008

Bioactivity guided phytochemical study of the petroleum ether and butanol extracts of Clematis hirsuta resulted in the isolation of 12 compounds. Rat paw edema as a model of acute inflammation was used to evaluate the anti-inflammatory activity of the extracts and the chromatographic fractions. Five known sterols and triterpenes namely: ${\beta}-amyrin$ (1), lupeol (2), ${\beta}-sitosterol$ (3), oleanolic acid (4) and stigmasterol glycoside (5) were isolated from the petroleum ether extract. The n-butanol extract afforded two compounds reported for the first time from natural source: (S)-(+)-dihydro-5-(hydroxymethyl)-2(3H)-furanone (7) and (s)-(-)-5-hydroxymethyl-2(5H)-furanone (8). In addition, anemonin (6), dihydro-4-hydroxy-5-(hydroxymethyl)-2(3H)-furanone (2-deoxy-D-ribono-1,4-lactone) (9), biophenol (cimidahurin) (10), glucose (11) and sucrose (12) were also identified. The structures were determined from spectroscopic data including 1D- and 2D-NMR experiments.

• Korean Journal of Pharmacognosy
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• v.9 no.2
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• pp.57-72
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• 1978

The constituents of Ranunculaceae plants are summerized from more than one hundred of papers. Fifteen steroidal alkaloids, thirty two of terpenoids (diterpenes and triterpenes), fifty four isoquinoline alkaloids are recorded. Thirty three papaverines, eighteen berberines, and three hydrastines belong to isoquinoline group. Besides, of alkaloids a number of glycosides are also found. Adonis glycoside, famous cardiotonics, from Adonis and cyanogenin glycoside and calthoside D were identified from the leaves of Thalictrum aquilegifolium and Caltha silvestris. Anemonin, the irritating substance, found in Pulsatilla grandiz $W_{ENDER}$. Kaempf-erol and dihydrokaempferol were isolated and identified from the leaves of Clematis brachyura ^M_{AXIMOWICZ}$ by author in 1968.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.343.2-343.2
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• 2002

Ranunculin (RAN). isolated from Ranunculaceae. exhibited significant cytotoxic activity against KB and Bel-7402 cells with ED$_{50}$ values of 0.21 and 0.35).${\mu}4M respectively. Under physiological condition. the ranunculin was deglycosylated to be protoanemonin. an active form containing ${\alpha}{\beta}$-unsaturated ketone moiety. which successively dimerized to be anemonin inactive form. (omitted)

• The Korean Journal of Pesticide Science
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• v.7 no.4
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• pp.248-257
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• 2003

The objective of this experiment was to search plant species with herbicidal activity in Korea. Two hundred native plants were collected and their methanol extracts were obtained. Herbicidal activity of methanol extracts were determined by seed bioassay using canola (Brassica napus L.) seedlings. Six plants such as Staphylea bumalda, Wistaria floribunda, Allium victorialis, Rumex crispus, Chionanthus retusa, and Ulmus parvifolia were highly herbicidal: their $GR_{50}$ values were < $1,000{\mu}g\;g^{-1}$. In addition, seventeen plants such as Galium spurium, Zelkova serrata, Campsis grandiflora, Eucommia ulmoides, Sorbus commixta, Deutzia glabrata, Cercis chinensis, Alnus hirsuta, Zanthoxylum schinifolium, Quercus acutissima, Robinia pseudoacacia, Gleditsia japonica, Kerria japonica, Ligustrum obtusifolium, Thuja orientalis, Chamaecyparis obtusa, and Pulsatilla koreana showed herbicidal activity: their $GR_{50}$ values were between 1,000 and $2,000{\mu}g\;g^{-1}$. However, 177 plants showed no herbicidal activity. Plants with herbicidal activity found in this study could be used for weed management and herbicidal compounds in such herbicidal plants could be used as lead compounds in the development of new herbicides.