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Characteristics of Six New Bacillus thuringiensis Serovarieties: B. thuringiensis serovar. coreanensis, leesis, konkukian, seoulensis, sooncheon, and yosoo

  • Lee, Kwang-Yong;Kwon, Hyuk-Han;Kang, Eun-Young;Lee, Min-Jung;Kim, Eui-Na;Chu, Dong-Wan;Park, Soo-Il;Ngo, Din-Binh;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.509-514
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    • 2004
  • Six new serovarieties of B. thuringiensis carrying specific H-antigen have minor differences in biochemical characteristics and morphological characteristics of crystals, which are commonly resistant against four antibiotics. The B. thuringiensis serovar. coreanensis is nontoxic to silkworm larvae, but it is moderately toxic against the Culex pipiens larvae. The B. thuringiensis serovar. konkukian and leesis are nontoxic against mosquitos larvae, but are toxic against silkworm larvae. The B. thuringiensis serovar. seoulensis, sooncheon, and yosoo are highly toxic to B. mori larvae and moderately toxic to C. pipiens larvae. The six serovarieties harbor different plasmid DNA patterns. A 102-kDa protein is a major crystal protein in the four serovarieties and a 86-kDa protein is in one serovariety.

Immunological Relationship of Crystal Proteins of Six Bacillus thuringiensis Serovarieties; B. thuringiensis serovar. coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41) andyosoo (H18a18c)

  • Lee, Kwang-Yong;Kang, Eun-Young;Kwon, Hyuk-Han;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.415-416
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    • 2004
  • Crystals of six new Bacillus thuringiensis serovarieties [coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41), and yosoo (HI8a18c)] with different H-antigens, which are toxic to Bombyx mori and/or mosquito larvae, were serologically quite distinct from each other.

A Study on the Carbamate Pesticide Residues in Domestic and Imported Crops in Chonnam Province (전라남도에서 유통중인 국내산 및 수입산 곡류 중의 카바메이트제 농약 잔류 실태 연구)

  • 김영국
    • The Korean Journal of Food And Nutrition
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    • v.13 no.2
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    • pp.164-170
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    • 2000
  • 10 carbamate pesticides were surveyed in domestic and imported green peas, beans, red beans from March 1999 through December 1999. Samples were collected from Gwangju, Mokpo, Suncheon and Yosoo grain markets. This study was performed by post-column ο-phthalaldehyde and 2-mercaptoethanol prior to HPCL fluorophore detection. Pesticides were found in 14 of 80 samples(17.5%). The kinds of pesticide detected in green peas were methomyl, MTMC, carbaryl, those in sesames were oxamyl, carbofuran, carbaryl, 1-naphthol, MIPC, those in beans were oxamyl, MTMC, carbaryl and those in red beans were MTMC, carbaryl. The range of residues detected in green peas, sesames, beans and red beans were 0.002∼0.025ppm, 0.001∼0.469ppm, 0.005∼0.356ppm and 0.017∼0.125ppm, respectively. The most frequently detected pesticides were MTMC(6 times) and carbaryl(5 times), while aldicarb, PHC and BPMC were no detected in all samples. Detection frequencies of carbamate pesticides in imported crops were higher than those in domestic ones except red beans. Only one result of oxamyl residues determined in imported bean was higher than the proposed maximum residue limits of Codex, which was 0.356ppm.

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Comparative Study on Maternal Health Status With Island and Land Women in A Middle City Area (중도시내(中都市內) 도서(島嶼)와 육지거주부인(陸地居住婦人)의 모성보건실태비교(母性保健實態比較))

  • Kim, Kyu-Chul
    • Journal of Preventive Medicine and Public Health
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    • v.9 no.1
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    • pp.65-75
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    • 1976
  • To compare the maternal health status between women in island and land, the study was conducted to adjacent land (kukdong) and island (kyonghodong) areas in Yosoo city during April 1976. The results obtained from 174 interviewee in island and 192 in land areas were the same as follow, 1. General characteristics of both area. Age: Land, group 20-29 year of age was the largest. Island, 30-39 year of age was the largest. Education; Low educated group, less than primary grade (land 88.6%, island 93.1%), was predominant in both area. Occupation of herself; agriculture and labor was the main occupation (land 50%, island 82.2%) in both area. Occupation of husband: labor and fishery was general (81.2%) in land, and agriculture and fishery was general (81.6%) in island. Duration of residence; Land, 25.0% being resided here more than 10 years. Island, 64.3% being resided here more than 10 years. 2. Marriage, Pregnancy, and Present children. Average age at marriage; Land, 20.7 years. Island, 20.9 years. Average frequency of pregnancy; Land, 4.4 times. Island, 4.3 times. Wastage of pregnancies: Land, 236 per 1000 pregnancies. Island, 151 per 1000 pregnancies. Wastage occupied by induced abortion; land, 73.5%. island, 60.5%. Number and sex of present children; Land, 3.3 per family, sex ratio 52.4 to 47.6. Island, 3.6 per family, sex ratio 53.3 to 46.7. 3. Prenatal and postnatal care. Prenatal consutation: Land, 16.1% received by doctor or midwife. Island 9.2% received by doctor or midwife. Complications during last pregnancy; Land, 46.6% complained. Island, 51.1% complained. Return to work within 1 week after delivery; Land, 40.6%. Island, 50.6%. 4. Delivery environment Home delivery; Land, 97.4% Island, 97.3%. Delivery attdended by non medical professions at home; Land, 80.2% Island, 93.7%. Solo home delivery; Land, 13.0%, Island, 12.9%. Delivery attended by layman without taking any disinfective preparations; Land, 48.1%, Island, 49.1%. Material mainly used to cut umbilical cord at home; Land, scissors (97.4%). Island, scissora (98.8%). Cord cutting material used without giving any disinfective preparations; Land, 79.9%, Island, 84.0%. Delivery sheets used at home; Cement bag paper (land 50.0%, island, 31.3%). Vinyl sheets (land,17.5%, island, 27.6%). News paper (land, 3.2%. island, 11.7%). No sheets (land 19.5%. island, 12.9%). etc. Maternal Meal; Seaweed soup with rice was the most general in both area. (land 95.3%, island, 91.4%).

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Studies on the Spanish Mackerel Populations (1) Age Determination (삼치 자원에 대한 연구 (1) 연령사정)

  • Kim, Wan Soo
    • 한국해양학회지
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    • v.5 no.1
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    • pp.37-40
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    • 1970
  • This work concerns age studies of the spanish mackerel by vertebrae. The spanish mackerel samples were obtained mainly from Yosoo and Inchon regions during July to December, 1969. A total of 292 fish was collected during the present study. Except the vertebrae, the incremental growth is found to be vague or complicated on the most bony parts in spanish mackerel. The spanish mackerel has 50 vertebrae, and the size of vertebra varies according to its position. After several studies, therefore, sample centrum was selected from the 23rd vertebra throughout the present study. Sample centrum was then stained with Alizarin Red S. and examined under binocular stereoscopic microscope illuminated by reflected light. The surface of the centrum shows many ring-marks running parallel to the edge. The ring-marks were counted and the measurements of centrum radius (R) and radii of the ring-marks (r$\_$i/) were made by eyepiece micrometer. From available data, the average of each ring-mark radius was found to be; r$\_$1/=2.05mm, r$\_$2/=3.11mm, r$\_$3/=4.05mm, r$\_$4/=4.89mm, r$\_$5/=5.70mm and r$\_$6/=6.47mm, respectively. The mean fork length at the time of ring-mark formation was calculated to be; l$\_$1/=297.6mm, l$\_$2/= 390.1mm, l$\_$3/=472.2mm, l$\_$4/=545.5mm, l$\_$5/=616.2mm, and l$\_$6/= 683.4mm, respectively.

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