• 제목/요약/키워드: and leaf culture

검색결과 905건 처리시간 0.032초

Gaeumannomyces graminis var. tritici에 의한 벼 엽초(葉?) 썩음병 (Sheath Rot Dieseases of Rice Seedling Caused by Gaeumannomyces graminis var. tritici in Korea)

  • 성재모;이성찬;박종성
    • 한국균학회지
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    • 제10권4호
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    • pp.177-180
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    • 1982
  • Gaeumannomyces graminis var. tritici was first isolated from rice in 1981. Sheath rot of rice seedling in box culture for machine transplanting was produced by artificial inoculation of the causal organism. Especially sheath rot was developed from crown to leaf sheath above water level. Perithecia were usually formed in dead leaf sheaths and in PDA culture. They were black, globose, and immersed with obliquely erumpent neck. Asci were unitunicate with an apical ring and contained 8 spores. Ascospores were hyline, slender, $80{\sim}140\;um$ in length. Each spore showed $3{\sim}4\;septa.$ Based on morphological and cultura characteristics, the isolate from seeds was identified as Gaeumannomyces graminis (Sacc.) Arx & Olivier tritici J. Walker.

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한국산 구아바 잎 추출물을 첨가한 크래커의 항산화활성 (Antioxidative Activity of Crackers Made with a Guava(Psidium guajava Linn.) Leaf Extract Harvested in Korea)

  • 허예진;심기현;최해연;김순임
    • 한국식품조리과학회지
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    • 제26권2호
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    • pp.171-179
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    • 2010
  • The objectives of this study were to investigate the antioxidative activity of crackers made with a guava(Psidium guajava Linn.) leaf extract harvested in Korea. Guava leaf extraction using boiling water showed significantly higher antioxidative activities than extracting using 70% ethanol based on the higher total phenolic contents, FRAP, and ABTS assays(p<0.05). The crackers containing 1% guava leaf extract, and 0.075% BHT were stored at $63^{\circ}C$ for 7 days for the Schaal oven test, and the oxidative stability(AV, POV), antioxidative activity(DPPH, FRAP, ABTS assay), and sensory evaluation were compared. The crackers containing 1% guava leaf extract were found to have a higher oxidative stability than the control due to a lower acid value and peroxide value after 7 days of storage. The antioxidative activities of the crackers containing 1% guava leaf extract was the highest after 7 days as determined in the DPPH and ABTS assay, and was lower than crackers containing 0.075% BHT after 4 days as assessed by the FRAP assay. In the sensory evaluation, the crackers containing the 1% guava leaf extract had the highest scores in terms of taste, texture, and overall palatability than others at increasing storage time. As a result, the addition of 1% guava leaf extract harvested in Korea increased the antioxidative effect as well as the sensory acceptability of crackers.

Criteria of Nitrate Concentration in Soil Solution and Leaf Petiole Juice for Fertigation of Cucumber under Greenhouse Cultivation in Gyeonggi region

  • Park, Jung-Soo;Roh, Ahn-Sung;Jang, Jae-Eun;Kang, Chang-Sung;Kim, Hee-Dong
    • 한국토양비료학회지
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    • 제48권4호
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    • pp.295-304
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    • 2015
  • To develop a technique for efficient management of fertility for cucumber in greenhouse, a quick test method to quantify nitrate ($NO_3{^-}$) content in soil solution and leaf petiole juice using a simple instrument that are easy to use for farmers was investigated. N fertilizer (urea) was applied at 0, 50, 100 and 200% levels of the recommended application rate from 30 days after transplanting to harvest by soil fertigation treatments. Stable results were obtained from analysis of nitrate ($NO_3{^-}$) using top $10^{th}$ or $11^{th}$ leaf petioles collected between 10 to 11 am in the morning. Under the semiforcing culture, $NO_3{^-}$ content of leaf petiole juice was highest at 60 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$content of leaf petiole juice was $2,418{\pm}78{\sim}2,668{\pm}118$ at 45 DAT, $3,032{\pm}90{\sim}3,332{\pm}63$ at 60 DAT, $2,709{\pm}50{\sim}3,158{\pm}155$ at 75 DAT, $2,535{\pm}49{\sim}2,907{\pm}83$ at 90 DAT, and $2,242{\pm}48mg\;L^{-1}$ at 105 DAT. In addition, appropriate $NO_3{^-}$ content of soil solution was $167{\pm}9{\sim}212{\pm}15$ at 45 DAT, $83{\pm}10{\sim}112{\pm}12$ at 60 DAT, $49{\pm}3{\sim}92{\pm}6$ at 75 DAT, $71{\pm}9{\sim}103{\pm}9$ at 90 DAT, and $73{\pm}9mg\;L^{-1}$ at 105 DAT. The cucumber yield at 100% N level of fertigation was $7,770kg\;10a^{-1}$ and no difference in yield was found at 200% N level of fertigation. However, there was 12% decrease in yield at 50% N fertigation and, 17% decrease at 0% N fertigation. Under retarding culture, $NO_3{^-}$ concentration of leaf petiole juice was highest at 55 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$ content of leaf petiole juice was $2,464{\pm}102{\sim}2,651{\pm}33$ at 45 DAT, $3,025{\pm}71{\sim}3,314{\pm}84$ at 55 DAT and $2,488{\pm}92mg\;L^{-1}$ at 65 DAT, respectively. Appropriate $NO_3{^-}$ content of soil solution was $111{\pm}10{\sim}155{\pm}14$ at 45 DAT, $93{\pm}7{\sim}147{\pm}14$ at 55 DAT, $67{\pm}4mg\;L^{-1}$at 65 DAT, respectively. The cucumber yield at 50% N fertigation was not different from $1,697kg\;10a^{-1}$ of 100% N fertigation level and even with that of the 200% N fertigation. However, there was 21% decrease in yield at 0% N fertigation.

마늘 생장점 조직배양 후 포장에서의 년차별 생육 및 수량특성 변화 (Yield change of seed bulb according to annual field culture after induced meristem culture in garlic (Allium sativum L.))

  • 권영석;최인후;김철우;최민선;곽정호;임용표
    • 농업과학연구
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    • 제42권4호
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    • pp.305-311
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    • 2015
  • In this research, we surveyed the yield change of garlic cultivation with seed bulbs according to annual field culture after induced meristem culture. From 2007 to 2013, yearly produced seed bulbs from $2^{nd}$ to $8^{th}$ generation were cultivated in the Muan field. Over the generations, the general growth characteristics such as the plant height, leaf number, leaf length and stem diameter were all decreased. When we compared the conventional seed bulbs and TCR ones, the plant height and stem diameter showed bigger difference. And the TCR seed bulbs showed a week late harvest time against the conventional ones. The yield of $2^{nd}$ generation was 72.3 g and 57.1 g for the $8^{th}$ generation. However every generation showed higher yield than conventional seed garlic's ones as 43.9 g. The yield index showed the same trend as $2^{nd}-8^{th}$ to 63% to 21%. After the tissue culture of garlics, the viral infections (OYDV and GSV) of TCR was far less than that of conventional ones. In case of LYSV, the infection ratio was 6.7% at $2^{nd}$ generation and 26% at $8^{th}$. However, the ratio was far less than that (80%) of the conventional ones.

한국차도(韓國茶道)의 구조적(構造的) 특성(特性) (A Study on the Structural Characteristics of Teaism of Korea)

  • 김명배
    • 한국식생활문화학회지
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    • 제1권1호
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    • pp.55-65
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    • 1986
  • In order to Characterizing the tea-culture of Korea, historical records for tea-culture of Korea, Japan and China were reviewed. The results were summarized as follows. 1. As for the type of tea through the history of Korea, it could be characterized as Cake-tea(餠茶) in Sam Kook dynasty, Iump-tea(硏膏茶) in Koryo dynasty and leaf tea(葉茶) in Chosun dynasty. Those were affected by Chinese and Japanese tea-culture. 2. Fashiono of tea-culture in Korea were unigue, imitative and reconstitutive. 3. It was concluded that the tea-culture in Korea might be established by the 'cult of tree' (樹木崇拜信仰) of Hwa Rang in Shilla dynasty.

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퇴비단 여과액비가 수경재배에서 상추의 생육과 수량에 미치는 영향 (Effects of Compost Leachate on Growth and Yield of Leaf Lettuce in Hydroponic Culture)

  • 류종원
    • 한국축산시설환경학회지
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    • 제15권1호
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    • pp.51-58
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    • 2009
  • 본 연구는 수경재배에서 여과액비의 양액대체 가능성을 평가하기 위하여 양돈농가의 퇴비화 과정 중 여과되어 나오는 퇴비단여과액비(SCB액비)와 화학양액의 혼합 시용이 상추(Lactuca sativa L.)의 생육에 미치는 영향를 검토하기 위하여 수행하였다. 본 연구에 사용된 수경재배 방식은 박막순환 수경재배(NFT방식) 방식으로 실시하였다. 처리는 상추의 수경재배에서 질소함량을 기준으로 여과액비와 양액의 혼합수준 처리구를 두었다. 처리구는 여과액비(CL 100%) 단독처리구, 여과액비 80% + 양액 20%(CL80% + NS20%) 혼합처리구, 여과액비 60% + 양액40%(CL 60% + NS 40%) 혼합처리구, 여과액비 40% + 양액60%(CL 40% + NS 60%) 혼합처리구, 여과액비 20% + 양액80%(CL 20% + NS 80%) 혼합처리구, 양액 100%(NS 100%) 단독처리구를 두었다. 여과액비와 양액혼합액은 전기전도도와 pH를 조정하여 상추의 양액재배를 실시한 결과를 요약하면 다음과 같다. 1. 여과액비는 칼리함량이 상대적으로 높고 질산태질소, 인산 함량이 낮아 여과액비 100% 처리구는 양분의 불균형을 초래하여 양액재배에서 상추의 지상부의 생육이 지연되어 대조구인 양액 대비 60%의 수량을 나타내어 수량감소가 있었으나 상추유기 농 수경재배의 가능성을 제시하였다. 2. 질소기준으로 여과액비와 양액을 40%;60% 혼합시 양액 대비 10%의 수량감소가 있었으나, 여과액비와 양액을 20%:80% 혼합하여 처방 처리 할 경우 대조구인 표준양액 처리와 대등한 수량을 나타내었다. 이상의 결과에서 여과액비와 양액을 질소기준으로 60% 이상 혼합 할 때 양분불균형이 해소되어 화학양액 100%에 근접하는 수량을 나타내어 여과액비 수경재배 활용시 40%의 화학양액을 절감하는 효과를 기대 할 수 있을 것으로 판단된다.

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방울토마토의 여름재배시 적정수경재배방식 구명 (The investigation of Appropriate Hydroponic System for Cherry Tomatoes in Summer Season)

  • 김영식
    • 생물환경조절학회지
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    • 제2권1호
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    • pp.53-57
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    • 1993
  • 방울토마토에 적합한 수경재배방식에 관한 연구 결과, 담액수경에서 주경의 기부직경, 엽장, 엽폭, 화방길이 등의 생육이 가장 좋았으며, 암면재배와 NFT는 유사하였다. 개화시기와 과실의 착색일에 대하여는 각 방식마다 차이를 보이지 않았으나, 과실의 수확량은 담액수경에서 많았다. 건물율, 유기산, 경도, 당도 등은 담액수경에서 낮았고, 당산비와 vitamin C는 높아 담액수경에서 품질이 약간 우수한 것으로 나타났다. 따라서, 방울토마토의 여름재배에서는 담액수경방식이 바람직한 것으로 평가되었다.

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실험실에서 사육한 말똥성게 Strongylocentrotus pulcherrimus (A. Agassiz)의 식성과 성장 (Feeding Habits and Growth of the Sea Urchin, Strongylocentrotus pulcherrimus (A. Agassiz) Reared in the Laboratory)

  • 허성범
    • 한국양식학회지
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    • 제1권2호
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    • pp.121-133
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    • 1988
  • 고가의 식품으로 이용되는 말똥성게의 생식소의 소비가 증가됨에 따라 연안의 말똥성게 자원은 계속 감소하고 있어 이 종류에 대한 양식 기술개발은 중요한 연구 과제이다. 따라서 본 연구는 말똥성게를 실내에서 양식할 경우 먹이로서 신선한 해조류를 대치할 수 있는 육상식물을 조사하였다. 해조류 6종류 (미역, 구멍갈파래, 지충이, 개지누아리, 모자반, 곰피), 육상식물 5종류 (시금치, 상치, 무우잎, 배추, 케일), 동물성 먹이 2종류 (바지락. 쇠고기)를 대상으로 말똥성게의 선택적 및 비선택적 식성과 영양 효과를 비교한 결과, 말똥성게의 먹이로서 육상 식물의 이용이 가능하였다. 미역을 공급한 경우 말똥성게의 체중의 성장은 가장 높았으나 생식소지수는 시금치, 무우잎 또는 상치를 공급할 경우가 미역의 경우보다 더 높았다. 시금치와 무우잎의 공급은 생식소가 성장하는 계절에 적합하고, 각이 성장하는 시기에는 상치 또는 배추가 효과적일 것으로 판단된다. 특히 무우잎은 식용으로 이용하지 않고 버려지므로 무우잎을 이용한 말똥성게의 양식은 매우 경제적일 것이다.

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수영 (Rumex acetosa L.) 암.수 체세포클론에서 염색체 변이 (Chromosomal Variation in Female and Male Somaclones of Rumex acetosa L.)

  • 김수영;이미경;김동순;방재욱
    • 식물조직배양학회지
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    • 제28권2호
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    • pp.113-116
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    • 2001
  • 성염색체의 조성이 다른 수영 (Rumex acetosa L.) 암·수 개체의 잎 절편 배양을 통하여 얻은 체세포클론을 대상으로 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 암 개체의 경우 조사된 25개체 중 21개체 (84%)가 야생형과 같은 염색체 조성 (2n=14)을 보였으며, 야생형에서는 관찰되지 않는 4배체 식물이 4개체 (16%)가 관찰되었다. 그에 비하여 수 개체에서는 20개체 중 4개체 (20%)만이 정상의 염색체 조성을 보여 성에 따른 체세포클론 변이에서의 차이를 보였다. 수 개체에서는 2n=14, 28 등의 이수체가 관찰되었으며, 4배체 (2n=30)로 나타난 체세포클론에서도 성염색체의 뚜렷한 배가현상이 관찰되지 않았다.

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Plant Regeneration from Leaf Explants of Kalanchoe daigremontiana Hamet & Perrier

  • ;Kim, Teh-Ryung;In, Jun-Gyo;Yang, Deok-Chun;Choi, Kwan-Sam
    • 한국약용작물학회지
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    • 제14권5호
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    • pp.293-298
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    • 2006
  • Optimum culture conditions for high frequency plant regeneration from leaf explants of Kalanchoe daigremontiana Hamet &Perrier were established. Shoot regeneration was achieved from leaf explant cultures using MS medium supplemented with indole-3-acetic acid (IAA) and thidiazuron (TDZ) or benzyladenine (BA). Percent regeneration was influenced by plant growth regulators and source of explants. MS medium supplemented with TDZ (1.0 mg/l) and IAA (0.4 mg/l) was the most effective, providing shoot regeneration for 76.7 % of ex vitro leaf explants associated with a high number of shoots per explant (9.5 mean shoots per explant), whereas 100% shoot regeneration associated with 12.4 shoots per explant occurred from in vitro leaf explants on the same medium. Clusters of shoots were multiplied and elongated on MS medium containing several concentrations of BA. MS medium supplemented with 0.25 mg/l BA was proved as the most effective shoot elongation medium. Elongated shoots (2-3 cm) were rooted at 100% on half-strength MS medium. Rooted plantlets were then transferred to potting soil. Regenerated plants were established in the soil with 90% success.