• Title/Summary/Keyword: and Zea mays

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Studies on Corn-Legume Intercropping System IV. Effects of corn-soybean intercropping on chemical composition and TDN yield (Silage용 옥수수와 두과작물의 간작에 관한 연구 IV. Silage용 옥수수 (Zea mays L.) 와 콩 ( Glycine max (L.) Merr.) 의 간작이 영양성분함량 및 TDN수량에 미치는 영향)

  • 이성규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.9 no.2
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    • pp.113-118
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    • 1989
  • This experiment was carried out to compare chemical composition, TDN yield of corn-soybean intercropping and corn monocropping forage plants at different harvesting time and obtained the following results. 1. In both cropping systems, the content of chemical composition of forage were changed same pattern in growing stage. The content of crude protein in corn-soybean intercropping forage at yellow stage increased more than that of corn nonocropping forage, while the crude fat in corn monocropping forage plants increased than that of corn-soybean intercropping forage plants at mature stage. 2. The crude fiber, crude ash, ADF content of forage plants in both cropping system decreased same pattern in growing period, however, NFE content of forage increased with maturity. 3. TDN yield of corn-soybean intercropping and corn monocropping forage plants at yellow stage obtained similar results and TDN yield per 10a in intercropping and monocropping were 1006.lkg and 978.6kg, respectively. 4. Consequently, corn-soybean interaopping system could be increased crude protein yield without decreasing of dry matter yield in comparison with corn monocropping system for corn silage.

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Functional Characterization of 5-Enopyruvylshikimate-3-Phosphate Synthase from Alkaliphilus metalliredigens in Transgenic Arabidopsis

  • Xing, Xiao-Juan;Tian, Yong-Sheng;Peng, Ri-He;Xu, Jing;Zhao, Wei;Yao, Quan-Hong;Sun, Sheng
    • Journal of Microbiology and Biotechnology
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    • v.24 no.10
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    • pp.1421-1426
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    • 2014
  • Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based twostep DNA synthesis method to synthesize an aroA gene ($aroA_{A.\;metalliredigens}$) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new $aroA_{A.\;metalliredigens}$ gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.

Vascular Plants of Mt. Jangsan in Busan Metropolitan City (부산광역시 장산 일대의 관속식물상)

  • Oh, Hyun-Kyung;Kim, Eun-Ok;Choi, Song-Hyun;You, Ju-Han
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.24 no.3
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    • pp.49-68
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    • 2021
  • This study was to carried out to survey and analyse the vascular plants of Mt. Jangsan, Busan Metropolitan City, South Korea. The vascular plants were surveyed from June to October, 2019. The numbers of vascular plants in this site were 497 taxa and comprised of 106 families, 306 genera, 454 species, 7 subspecies, 29 varieties, 5 forms and 2 hybrids. The planted species were 16 taxa including Ginkgo biloba, Cedrus deodara, Zea mays and so on. In this study, 309 taxa were identified, including Selaginella rossii, Cyrtomium falcatum, Cephalanthera falcata and so on. The rare plants were 4 taxa including Hololeion maximowiczii(EN), Ottelia alismoides(LC), Iris ensata Thunb. var. spontanea(LC) and Sagittaria trifolia(DD). All rare plants were found in Jangsan Wetland. The Korean endemic plants were 9 taxa including Populus × tomentiglandulosa, Hemerocallis hakuunensis, Hosta minor and so on. The floristic target species were totally 53 taxa, which were 2 taxa of grade IV, 11 taxa of grade III, 6 taxa of grade II and 34 taxa of grade I. The alien plants were 58 taxa including Rumex crispus, Ipomoea purpurea, Helianthus tuberosus, Vulpia myuros and so on.

Characterization of Lupinus Iuteus Chloroplgsl Gene Coding for Components of a Chloroplastic NADH Dehydrogenase

  • Oczkowski, Marian;Augustyniak, Halina
    • Journal of Plant Biotechnology
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    • v.2 no.2
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    • pp.73-78
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    • 2000
  • The plastid genomes of several plants contain ndh genes homologues of genes encoding subunits of the mitochondrial complex I. We sequenced the part of lupin ndhB, ndhD and ndhF genes in order to compare the structure of these genes with those of Nicotiana tabaum, Arabidopsis thaliana, Zea mays and Oryza sativa with the idea to detect the presence of stretches with identical aminoacid composition. We were only able to find one or two stretches of this kind of about 16 aminoacid- long in the analyzed fragments of the ndh genes. The total number of such stretches was different in particular gene products: for ndhc 1, ndhB 9, ndhD 3 and ndhF 6. We have also examined the transcription pattern of ndhC, ndhK and ndhJ genes during lupin development. We show that the greatest amount of ndhC, ndhK and ndhJ transcripts are observed in 7- to 14 day- old lupin seedlings. We also studied the level of transcription of those genes in plants growing at low temperature. All the data confirmed that the abundance of transcription of ndhC, ndhK, and ndhJ genes increased under chill conditions. It has to be noted that the level of transcription of the ndhC gene was higher than the other genes probably due to higher stability of this transcript.

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The Density Effect on the Dry Matter Production of Zea mays (옥수수의 건물질성산에 미치는 밀도효과)

  • 이일구
    • Journal of Plant Biology
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    • v.13 no.1
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    • pp.56-64
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    • 1970
  • The present experiments differning from that conducted by agriculturists with the first consideration of food or seed production were performed in order to investigate the density effect on dry matter production in the artified population of maize. Three experimental plots were established and one seed was sown in the first plot, 2 seeds in the second plot and 3 seeds in the third plot, respectively. The space of each ridge was designed with 30cm in width and 50cm in length. The space of each seed was 4cm and the growth amount by density was measured through three times. The results obtained are as follows: 1) The total growth amount per unit area was in the order of the third, second and first plots until the second sampling. At the third sampling the whole plots showed almost the same growth amount except the first plot which showed a slightly smaller amount. 2) The growth amount per individual plant was in the order of the third, second and first plots until the second sampling, while the first plot showed drastic growth compared with the other two plots at the third sampling. 3) The growth amount by each organ(leaf, stem and root) of the plant body was markedly abundant in the stem of the non-productive part compared with that in the leaf of the productive part. Especially the root was affected remarkably by density and the first plot showed an abundant growth in comparison with the other plots which were too poor to resist the gound surface.

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Effect of Herbicide Combinations on Bt-Maize Rhizobacterial Diversity

  • Valverde, Jose R.;Marin, Silvia;Mellado, Rafael P.
    • Journal of Microbiology and Biotechnology
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    • v.24 no.11
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    • pp.1473-1483
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    • 2014
  • Reports of herbicide resistance events are proliferating worldwide, leading to new cultivation strategies using combinations of pre-emergence and post-emergence herbicides. We analyzed the impact during a one-year cultivation cycle of several herbicide combinations on the rhizobacterial community of glyphosate-tolerant Bt-maize and compared them to those of the untreated or glyphosate-treated soils. Samples were analyzed using pyrosequencing of the V6 hypervariable region of the 16S rRNA gene. The sequences obtained were subjected to taxonomic, taxonomy-independent, and phylogeny-based diversity studies, followed by a statistical analysis using principal components analysis and hierarchical clustering with jackknife statistical validation. The resilience of the microbial communities was analyzed by comparing their relative composition at the end of the cultivation cycle. The bacterial communites from soil subjected to a combined treatment with mesotrione plus s-metolachlor followed by glyphosate were not statistically different from those treated with glyphosate or the untreated ones. The use of acetochlor plus terbuthylazine followed by glyphosate, and the use of aclonifen plus isoxaflutole followed by mesotrione clearly affected the resilience of their corresponding bacterial communities. The treatment with pethoxamid followed by glyphosate resulted in an intermediate effect. The use of glyphosate alone seems to be the less aggressive one for bacterial communities. Should a combined treatment be needed, the combination of mesotrione and s-metolachlor shows the next best final resilience. Our results show the relevance of comparative rhizobacterial community studies when novel combined herbicide treatments are deemed necessary to control weed growth.

An efficient microscopic technique for aleurone observation with an entire kernel cross-section in maize (Zea mays L.)

  • Jae-Hong Kim;Ji Won Kim;Gibum Yi
    • Korean Journal of Agricultural Science
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    • v.50 no.4
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    • pp.645-652
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    • 2023
  • The aleurone layer in maize is crucial as it contains essential nutrients such as minerals, vitamins, and high-quality proteins. While most of the maize varieties are known to possess a single aleurone layer, several multi-aleurone layer mutants and landraces have been suggested for hierarchical genetic control of aleurone development. Conventional microscopy analysis often involves using immature seeds or sampling only a portion of the kernel sample, and whole kernel section analysis using a microtome is technically difficult and time-consuming. Additionally, the larger size of maize kernels posed challenges for comprehensive cross-sectional analysis compared to other cereal crops. Consequently, this study aimed to develop an efficient method to comprehensively understand the aleurone layer characteristics of the entire cross-section in maize. Through observations of diverse maize genetic resources, we confirmed irregular aleurone layer patterns in those with multiple aleurone layers, and we discovered a landrace having multiple aleurone layers. By selectively identifying genetic resources with multiple aleurone layers, this method may contribute to efficient breeding processes in maize.

A Gene Encoding $\beta$-amylase from Saprolegnia parasitica and Its Expression in Saccharomyces cerevisiae

  • Kim, Hee-Ok;Park, Jeong-Nam;Shin, Dong-Jun;Lee, HwangHee Blaise;Chun, Soon-Bai;Bai, Suk
    • Journal of Microbiology and Biotechnology
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    • v.11 no.3
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    • pp.529-533
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    • 2001
  • The ${\beta}$-Amylase cDNA fragment from the oomcete Saprolegnia parasitica was cloned by reverse transcription-polymerase chain reaction (RT-PCR) using degenerate oligonucleotide primers derived from conserved ${\beta}$-amylase sequences. The 5'and 3'regions of the $\beta$-amylase gene were amplified using the rapid amplification of cDNA ends (rACE) system. It consisted of an open reading frame of 1,350 bp for a protein of 450 amino acids. Comparison between the genomic and cDNA sequences revealed that the intron was not present in the coding region. The deduced amino acid sequence of the ${\beta}$-amylase gene had a 97% similarity to the ${\beta}$-amylase of Saprolegnia ferax, followed by 41% similarity to those of Arabidopsis thaliana, Hordeum vulgare, and Zea mays. The ${\beta}$-amylase gene was also expressed in Saccharomyces cerevisiae by placing it under the control of the alcohol dehydrogenase gene (ADC1) promoter.

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Solid Culture of Phosphate-solubilizing Fungus, Penicillium sp. PS-113 (인산가용화 사상균 Penicillium sp. PS-113의 고체배양)

  • Kang, Sun-Chul;Choi, Myoung-Chul
    • Microbiology and Biotechnology Letters
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    • v.27 no.1
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    • pp.1-7
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    • 1999
  • A fungus, Penicillium sp. PS-113, isolated from soil showed the high phosphate-solubilizing activity in patato dextrose broth-rock phosphate to produce free phosphates to the culture broth with the concentrations of 585 ppm against rock phosphate. In this medium, the optimum temperature and initial pH to solubilize rock phosphate were 30$^{\circ}C$ and pH 7.5, respectively. In order to make the mass production of the conidia from this fungus, we cultured in on various solid-based media like barley, corn, wheat, rice, rice bran, and compost. As a result, the fungus highly produced conidia ranging from 2.1 to $5.1{\times}10_9$ conidia/g${\cdot}$media on these solid media except compost-based medium, which was 0 times less than others. Effects of inoculation of the phosphate solubilizing fungus as a biofertilizer were studied in perlite-based pot cropped with Zea mays Suwon 19. Inoculation of Penicillium sp. PS-113 increased in plant height (1.4 times), plant weight (5.2~8.1 times) and root length (1.1~1.2 times) at 60-day cultivation, compared to Hogland solution either without $NH_4H_2PO_4$ or displace $NH_4H_2PO_4$ to powdered rock phosphate, a phosphorus source for plant growth.

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Isolation and Identification of Short Term Drought-Induced Genes in Zea mays L. Leaves

  • Rahman, Md. Atikur;Lee, Sang-Hoon;Choi, Gi Jun;Ji, Hee Jung;Kim, Won Ho;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.3
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    • pp.237-241
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    • 2017
  • Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.