Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Characterization of Lupinus Iuteus Chloroplgsl Gene Coding for Components of a Chloroplastic NADH Dehydrogenase

  • Oczkowski, Marian (Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poland) ;
  • Augustyniak, Halina (Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poland)
  • Published : 2000.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

The plastid genomes of several plants contain ndh genes homologues of genes encoding subunits of the mitochondrial complex I. We sequenced the part of lupin ndhB, ndhD and ndhF genes in order to compare the structure of these genes with those of Nicotiana tabaum, Arabidopsis thaliana, Zea mays and Oryza sativa with the idea to detect the presence of stretches with identical aminoacid composition. We were only able to find one or two stretches of this kind of about 16 aminoacid- long in the analyzed fragments of the ndh genes. The total number of such stretches was different in particular gene products: for ndhc 1, ndhB 9, ndhD 3 and ndhF 6. We have also examined the transcription pattern of ndhC, ndhK and ndhJ genes during lupin development. We show that the greatest amount of ndhC, ndhK and ndhJ transcripts are observed in 7- to 14 day- old lupin seedlings. We also studied the level of transcription of those genes in plants growing at low temperature. All the data confirmed that the abundance of transcription of ndhC, ndhK, and ndhJ genes increased under chill conditions. It has to be noted that the level of transcription of the ndhC gene was higher than the other genes probably due to higher stability of this transcript.

Keywords

References

  1. Proc Natl Acad Sci USA v.79 Evidence for a respiratory chain in the chloroplast Bennoun P
  2. Planta v.190 Studies on the expression of mdhH, a subunit of the NAD(p)H-plastoquinone-oxidoreducatase of higher-plant chloroplasts Berger S;Ellersiek U;Westhoff P;Steinmuller K
  3. PCC6803. FEBS Lett v.326 Immunopurification of a subcomplex of the NAD(P)H-plastoquinone- oxidoreductase from the cyanobacterium Synechocystis sp. Berger S;Ellersiek U;Kinzelt D;Steinmuller K
  4. Plant Cell Physiol v.36 Properties of a large complex with NADH dehydrogenase activity from barley thylakoids Cuello J;Quiles MJ;Albacete ME;Sabater B
  5. The EMBO J v.17 Identification of a functional respiratory complex in chloroplatsts through analysis of tobacco mutants containing distrupted plastid ndh genes Burrows PA;Sazanow LA;Svab Z;Maliga P;Nixon PJ
  6. Plant Physiol v.109 Differential Transcription of peachloroplast genes during light-inducet leaf development. Continuous far - red light activates chloroplast transcription Dubell AN;Mullet JE
  7. Thee EMBO J v.17 Chloroplast NADH dehydrogenase from Pisum sativium: characterization of its activity and cloning of ndhk gene Elortz F;Asturiaz JA;Arizmendi JM
  8. FEBS Lett v.457 The role of chloroplastic NAD(P)H dehydrogenase in photoprotection Endo T;Shikanai T;Takabayashi A;Asada K;Sato F
  9. Plant Physiol v.116 Nonphotochemical reduction of the plastoquinone pool in sunflower leaves originates from chlororespiration Feild TS;Nedbal L;Ort DR
  10. Planta v.179 Respiratory control over photosynthetic electron transport in chloroplast of higher plant: evidence for chlororespiration Garab G;Lajk F;Mustrdy L;Mrton M
  11. Biochim Biophys Acta v.1188 Light-induced oxygen uptake in tobaco chloroplasts explained in terms of chlororespiratory activity Gruszecki WI;Bader KP;Schmid GH
  12. Mol Gen Genet v.258 Mutagenesis of the genes encoding subunits A, C, H, I, J and in tobaco by polyenthylene glyol- mediated plastome transformation Kofer W;Koop HU;Wanner K;Steinmuller K
  13. J Mol Biol v.6 A simple for displaying the hydropatic character fo a protein Kyte J;Doolittle RF
  14. Plant Science v.127 Cloning and transcription analysis of the ndhC-ndhK-ndhJ genes of lupin plastid DNA Oczkowski M;Paszkiewicz J;Piatyszek M;Augustyniak H
  15. Mol Gen Genet v.210 Six chloroplast genes (ndh A-F) homologous to human mitochondrial genes encoding components of the respiratory chain NADH dehydrogenase are actively expressed: Determination of the splice sites in ndhA and ndhB premRNAs Matsubayshi T;Wakasugi T;Shinozaki K;Yamaguchi-Schinozaki K;Zaita N;HIdaka T;Yuan Meng B;Ohto Ch;Tanaka M;Kato A;Maruyama T;Sugiura M
  16. Plant Science v.123 Plastid DNA of five ecotypes of Arabidopsis thaliana : sequence of ndhG gene and maternal inheritance Martinez P;Lopez C;Roldan M;Sabater B
  17. Biochim Soc Trans v.24 Detection and characterization of complex I-like NADH- specific dehydrogenase from pea thylakoids Martinez P;Lopez C;Roldan M;Sabater B
  18. FEBS Lett v.429 the chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves Sazanov LA;Burrows PA;Nixon PJ
  19. Proc Natl Acad Sci USA v.95 The plasted ndh genes code for an NADH- specific dehydrogenase: isolation of complex I analogue from pea thylakoid membranes Sazanov LA;Burrows PA;Nixon PJ
  20. Mol Gen Genet v.216 Characterization of the ndhC-psbG-ORF 157/159 operon of maize DNA and of the cyanobacterium Synechocystis sp. PCC6803 Steinmller K;Ley AC;Steinmetz AA;Sayre RT;Bogorad L