• Korean Journal of Pharmacognosy
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• v.18 no.3
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• pp.168-176
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• 1987

Veronicastrum sibiricum (L.) Pennell (Scrophulariaceae) is a perennial herb growing in moist land. It has been used as a korean folk medicine in treating common cold, leucorrhea, cystitis and liver damage and as cholagogue. The present study dealt with the elucidation of the chemical components. From the roots of the plant, five iridoids were isolated and identified as minecoside $(mp\;142{\sim}3^{\circ})$, 6-O-veratryl catalpol ester $(mp\;216{\sim}8^{\circ})$, catalpol $(mp\;204{\sim}6^{\circ})$, aucubin $(mp\;180{\sim}2^{\circ})$ and 6-desoxy-8-isoferuloyl harpagide $(mp\;139{\sim}41^{\circ})$. Furthermore, ${\beta}-sitosteryl-3-O-D-glucoside$, campesteryl 3-O-D-glucoside, ${\beta}-sitosterol$, campesterol, stigmasterol and mannitol were also isolated.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.8-14
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• 2001

The effects of media, cholesterol, $\beta$-sitosterol and lecithin on the growth and oospore production of the isolates KM10, U6, CDB6, MHB6, JD1 (A$^2$type) of Phytophthora infestans isolated in Korea and F8l7, DNC303 (A$^1$type), IB908, DN107 (A$^2$type) obtained from Japan were investigated. Mycelium of P. infestans grew better on V-8 juice agar and rye meal agar than on the other media. Oospores were produced most abundantly on V-8 juice agar. Mycelium extended more 16.6, 8.3, and 5.2% on V-8 juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively, and oospores are produced 76.0, 58.0, and 34.6 % on V-8juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively. Oospores more produced on detached potato plant disks when $A^1$ and $A^2$ type exist simultaneously which indicating that variation of population can occur in the field, but the rate of oospore formation and the number of oospores produced was low and small quantity.

• Natural Product Sciences
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• v.18 no.3
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• pp.183-189
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• 2012

It is well known that inflammation is associated with neurodegenerative disorders, including Alzheimer' disease, Parkinson's disease and ischemia. Nitric oxide (NO), a pro-inflammatory mediator, is produced by inducible NO synthase (iNOS) in microglia as well as macrophages and appears to account for neurodegeneration. In this study, we aimed to isolate NO inhibitors from Pyrus pyrifolia by activity guided purification. As a result, we identified daucosterol and ${\beta}$-sitosterol, which have not been isolated from this plant before. This article also describes NO inhibitory activities of the methanol extract of Pyrus pyrifolia fruit and the isolated compounds from this, which are lupeol, betulin, betulinic acid, ${\beta}$-sitosterol and daucosterol, in LPS-activated RAW 264.7 and BV2 cell lines. Western blot analysis was performed to clarify the underlying mechanism of NO inhibition in the two cell lines.

• Korean Journal of Pharmacognosy
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• v.25 no.1
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• pp.28-30
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• 1994

The rate of growth and production of bioactive substances from Rehmannia glutinosa Liboschitz (Scrophulariaceae) were studied with the variation on the constituents of the culture media. The best growth was observed from MS basal medium containing 3.0 ppm NAA and 2.0 ppm kinetin. Carbohydrates (fructose, glucose and sucrose), phytosterols(${\beta}-sitosterol$, campesterol and stigmasterol) and carotenoid like substances were identified by GC-MS and TLC from the callus mass. However, catalpol was not detected from both solid and cell suspension cultures containing geraniol. Callus cultured Rehmannia glutinosa in the MS basal medium containing 0.1 ppm NAA and 0.1 ppm kinetin become differentiated to root.

• Natural Product Sciences
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• v.10 no.2
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• pp.69-73
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• 2004

Continuation of a phytochemical study of Cichorium intybus L. (Astraceae) growing in Egypt, resulted in the isolation and identification of a new sesquiterpene lactone 3,4-dihydrolactucin, in addition to the eight known compounds; kaempferol, isoscutellarin, cichoriin, umbelliferone, lupeol, lupeol acetate, ${\beta}-sitosterol$, and ${\beta}-sitosterol-3-O-glucoside$. Chemical structures of the isolated compounds were assigned based on different physical, chemical, and spectroscopic techniques including IR, UV, MS, 1D- and 2D-NMR spectra. Furthermore, the antimicrobial, and spasmogenic activities of some fractions and isolates were also assessed.

• Natural Product Sciences
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• v.9 no.4
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• pp.270-272
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• 2003

The methanol extract of Euphorbia milii exhibited strong inhibitory effect on platelet aggregation in the cource of our search for anti-platelet component from succulent plants. Two components, components 1 and 2 were isolated from this plant. 1 was the mixture of 72% of 1-octacosanol (1a) and 28% of 1-triacontanol (1b), and 2 was identified as ${\beta}-sitosterol$. 2 ($IC_{50}$: $195\;{\mu}M$, and $170\;{\mu}M$ respectively) was about two fold stronger than ASA ($IC_{50}$: $420\;{\mu}M$ and $340\;{\mu}M$ respectively) on both collagen and U46619 induced aggregation, while the effect of 1 to platelets was negligible.

• Natural Product Sciences
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• v.8 no.1
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• pp.23-25
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• 2002

Ten compounds were isolated from the stem barks of Acanthopanax gracilistylus WW Smith (AGS) by steam distillation, they were p-menta-1,5,8-triene, n-butyl isobutylphthalate, p-mentha-1,5-diene-8-ol, 8-hydroxy-p-cymene, myrtenol, trans-(+)-carveol, 1,3-di-tert-butylbenzene, 4-methyl-2,6-di-butylphenol, valencene and verbenone, respectively, characterized by GS-Mass spectra. And we have also extracted and isolated from the MeOH extracts of the stem barks of AGS, two compounds were obtained. On the basis of chemical and spectral evidence, they were syringin(l), ${\beta}-sitosterol(2)$.

• YAKHAK HOEJI
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• v.22 no.4
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• pp.219-225
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• 1978

From the dried roots of Caragana chamlagu Lamarck which have been known to be effective against neuralgia, arthritis, migraine, and sputum, etc. in the folk medicines of Korea, a colorless sterol-like substance, C$_{29}H_{50}O$, m.p. $115-117^{\circ}$, was isolated from the non-saponified fraction of methanolic extract. It was confirmed to be a mixture of four sterols, namely cholesterol, brassicasterol, campesterol, and $\beta$-sitosterol by physico-chemical properties, ir, nmr, mass spectra and gas liquid chromatographic method with the comparison of the authentic samples. In mass fragments of four sterols, furthermore, the same ions of m/e 273, 255, 229 and 213 were assigned to fragments of stigmastane skeletons of the unsaturated phytosterol, and the characteristic ions of each sterol were observed 386, 371, 368, 353 in cholesterol, 398, 383, 380, 365, 355, 337 in brassicasterol, 400, 385, 382, 367 in campesterol and 414, 399, 396, 381 in $\beta$-sitosterol, respectively.

• Natural Product Sciences
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• v.19 no.2
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• pp.137-144
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• 2013

Phytochemical constituents were isolated from the seeds of tartary buckwheat (Fagopyrum tataricum) by open column chromatography. Their structures were elucidated as ${\beta}$-sitosterol (1), ${\beta}$-sitosterol-3-O-glucoside (2), oleanolic acid (3), kaempferol (4), quercetin (5), kaempferol-3-O-rutinoside (6), and quercetin-3-O-rutinoside (7) on the basis of spectroscopic analysis including $^1H$-, $^{13}C$-NMR, and MS. To our knowledge, oleanolic acid (3) has been isolated for the first time from the seeds of Fagopyrum species. The total contents of compounds 4 - 7 were 0.500 mg/g in Daesan maemil, 0.312 mg/g in Yangjul maemil, and 2.185 mg/g in tartary buckwheat.

• Korean Journal of Pharmacognosy
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• v.31 no.1
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• pp.1-6
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• 2000

Five compounds were isolated from the methanol extract of the tubers of Cyperus rotundus (Cyperaceae). They were identified as cyperol(1), ${\alpha}-cyperone(2)$, cyperotundone(3), sugetriol triacetate(4) and ${\beta}-sitosterol(5)$ on the basis of spectroscopic and physicochemical analysis. Among them, cyperol, ${\alpha}-cyperone$ and cyperotundone were shown inhibitroy effects on LPS-induced nitric oxide production in RAW 264.7 cells in a dose dependent manner.