• Journal of Microbiology and Biotechnology
• /
• v.19 no.5
• /
• pp.495-501
• /
• 2009

A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and $\beta$-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.

• Journal of the Korea Safety Management & Science
• /
• v.16 no.3
• /
• pp.55-62
• /
• 2014

During five years (Year 2009~2013), Total victims of 72 %(81,560 people) and those 50.6 %(1,258 people) of death accident occurs in small scale construction site which operate 2 million USD less construction budget. Especially, falling death accident account for 785 people, in the share of 33.2 %(261 people) death disaster takes due to defect of original cause materials. The major safety issues in small scale work place take place while scaffold installation, disassembling, work-plate improper installation or non-professional skills of workers. Furthermore, labor subcontract systems make small construction site shortage of resources. Those workers regard work-plate as unnecessary and consumables supplies. Because of that most of workers use unsafe workplace in most construction site. Therefore, in order to prevent falling accident occurring in small scale work site, government should organize related regulations such as "Work site safety construction method" and then expands education support, financial aid, and sourcing safety supplies for work plate which offer broad variety experiences. Also, introduce certificate solutions for various work plates to improve safety function such as anchoring type method and anti sliding function.

• Computers and Concrete
• /
• v.21 no.4
• /
• pp.441-449
• /
• 2018

FE models for complex or large-scaled structures that need detailed modeling of structural components are usually constructed using commercial analysis softwares. Updating of such FE model by conventional sensitivity-based methods is difficult since repeated computation for perturbed parameters and manual calculations are needed to obtain sensitivity matrix in each iteration. In this study, an FE model updating procedure avoiding such difficulties by using response surface (RS) method and a Pareto-based multiobjective optimization (MOO) was formulated and applied to FE models constructed with a commercial analysis package. The test building is a low-rise reinforced concrete building that has been seismically retrofitted. Dynamic properties of the building were extracted from vibration tests performed before and after the seismic retrofits, respectively. The elastic modulus of concrete and masonry, and spring constants for the expansion joint were updated. Two RS functions representing the errors in the natural frequencies and mode shape, respectively, were obtained and used as the objective functions for MOO. Among the Pareto solutions, the best compromise solution was determined using the TOPSIS (Technique for Order of Preference by Similarity to Ideal Solution) procedure. A similar task was performed for retrofitted building by taking the updating parameters as the stiffness of modified or added members. Obtained parameters of the existing building were reasonably comparable with the current code provisions. However, the stiffness of added concrete shear walls and steel section jacketed members were considerably lower than expectation. Such low values are seemingly because the bond between new and existing concrete was not as good as the monolithically casted members, even though they were connected by the anchoring bars.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.12 no.1
• /
• pp.151-157
• /
• 2008

It is difficult for existing beams to ensure the sufficient embedding depth due to constructional cause. Owing to these problems, currently FRP-Rod embedding repair method run parallel with a section increasing method in many case. In this study, anchor pin was adopted to solving the problems of the sliding of FRP-Rod and the unified behaviors of the repaired beam without increasing the section. In the experiments, split failure of covering concrete and slippage of the FRP-Rod were not occurred. it was confirmed that FRP-Rod shown the integrate behaviors with RC beam till the repaired beams destroyed.

• Smart Structures and Systems
• /
• v.5 no.1
• /
• pp.81-94
• /
• 2009

A vibration-impedance-based monitoring method is proposed to predict the loss of prestress forces in prestressed concrete (PSC) girder bridges. Firstly, a global damage alarming algorithm using the change in frequency responses is formulated to detect the occurrence of damage in PSC girders. Secondly, a local damage detection algorithm using the change in electro-mechanical impedance features is selected to identify the prestress-loss in tendon and anchoring members. Thirdly, a prestress-loss prediction algorithm using the change in natural frequencies is selected to estimate the extent of prestress-loss in PSC girders. Finally, the feasibility of the proposed method is experimentally evaluated on a scaled PSC girder model for which acceleration responses and electro-mechanical impedances were measured for several damage scenarios of prestress-loss.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.32 no.5
• /
• pp.382-386
• /
• 2019

The alignment characteristics of liquid crystal (LC) molecules on a solution-derived lanthanum zinc oxide (LZO) film under ion-beam irradiation were demonstrated. Using the solution process, an LZO film was fabricated on the glass substrate and cured at $100^{\circ}C$. Afterwards, ion-beam irradiation was performed following the LC alignment method. Using this film, an LC cell was fabricated and the characteristics of the LC alignment were verified. Cross polarizing microscopy and the crystal rotation method were used to investigate the alignment state of the LC molecules on the LZO films. Furthermore, field emission scanning electron microscopy and X-ray photoelectron spectroscopy were used to explore the effect of the ion-beam irradiation on the LZO film. Through these, it was confirmed that the ion-beam irradiation induced surface modification, which demonstrated anisotropic physical and chemical surface characteristics. Due to this, uniform LC alignment was achieved. Finally, the residual DC and anchoring energy of the LC cell based on the LZO films were measured using a capacitance-voltage curve.

• Journal of Korean Association for Spatial Structures
• /
• v.24 no.1
• /
• pp.29-36
• /
• 2024

The paper presents the experimental investigation of RC beams retrofitted with Textile Reinforced Mortar (TRM), featuring enhanced bond capacity. Anchoring systems, including an extension of retrofitting length and the use of chemical anchors, are newly employed to improve the structural performance of the RC beam retrofitted with TRM. For the experimental investigation, a total of seven shear-critical RC beams, with and without stirrups, were designed and constructed. The structural behaviors of specimens retrofitted with the proposed TRM methods were compared to those of non-retrofitted specimens or specimens strengthened with conventional TRM methods. Crack pattern, force-displacement relationship, and absorbed energy were evaluated for each specimen. The experimental results indicate a significant improvement in the shear capacity of the RC beam with the proposed retrofitting method. Therefore, it is concluded that the application of an extended retrofitting length and chemical anchors to the TRM retrofitting method can effectively enhance the bond capacity of TRM, thereby improving the shear performance of RC beams.

• The Journal of Engineering Geology
• /
• v.10 no.3
• /
• pp.205-216
• /
• 2000

An unstable natural slope located at Icheon-Ri, Dasa-Eup, Dalseong-Kun which has been severely cracked during heavy rain fall in September, 1998, was investigated and analysed for remedial measures. Various geological data were obtained by performing a surface geological survey, a ground penetrating radar(GPR) exploration, four geotechnical borings with field and laboratory tests, and the precipitation records were also reviewed. Based on the these data, the probable sliding plane was determined as a bedding plane with dip angle of 20 to 25 degrees between sandstone and shale layers at depth of 6 to 8 meters. By performing back analyses with the limit equilibrium method, the friction angle and cohesion of the sliding plane were determined as 18$^{\circ}$ and 0, respectively. Based on these results, a series of stability analyses such as stereo-graphic projection method, limiting equilibrium method and numerical method were carried out. The remedial measures for the unstable slope were selected based on the results of the limit equilibrium analyses by considering various conditions expected at the site. Ground grouting or anchoring method with proper surface drainage systems could be employed for the long term safety of the slope.

• Science of Emotion and Sensibility
• /
• v.12 no.1
• /
• pp.45-54
• /
• 2009

Categorization in a vital means for dealing with the multitudes of entities in the world surrounding people. Among others, the perceptual and the evaluative similarities factors strongly affect categorization. The conventional SD-type procedure are insufficient in this regard, since it requires an individual subject to make isolated judgments about each stimulus to identify categorization in terms of a group tendency. It disregards the individual categorization in which the similarities are of great importance. Thus in this study the phased emotional evaluation method is suggested based on the intuitive categorization of stimuli and on the similarity judgement of representative/ non-representative case in each category. To verify the effectiveness of the suggested evaluation method the scanned jewelry images are selected as test stimuli for emotional evaluation experiment. As a result of the evaluation experiment, the conventional SD-type procedure is complemented by the emotional evaluation method in phases of the task of intuitive categorization, the selection of the representative images and the setup of the evaluation score of the representative images to internally supplied anchors of evaluating non-representative images.

• Archives of Plastic Surgery
• /
• v.37 no.4
• /
• pp.504-508
• /
• 2010

Purpose: Traumatic telecanthus can result from nasoethmoid-orbital fractures. Repair of the medial canthal tendon (MCT) using transnasal wiring is regarded as a choice of method to treat telecanthus, however, is often complicated by incomplete anchoring and drift of canthus, extrusion of wire, in-fracture of orbital bone, and eye damage. The authors introduced oblique transnasal wiring method through the Hiraga's epicanthopalsty incision instead of well-known classical bicoronal approach. Methods: Five patients with traumatic telecanthus were treated with this method. Though the Hiraga's epicanthoplasty incision, we could approach the operative field; the medial orbital wall and detached MCT. Oblique transnasal wiring was performed as following steps. After slit skin incision on the contralateral nasal recession area, drill holes were made from this point to the superior and posterior point of lacrimal sac of deformed eye. A 2-0 wire was double-passed through the holes and MCT. Traction was applied to ensure pulling the MCT and the wires were twisted in the contralateral nose, securing the MCT in the correct position. Results: All patients except 1 person showed improvement and rapid recovery. On average each canthus was moved 5.6 mm medially. In all cases, there were no eyelashes disappear, lacrimal canaliculitis, lacrimal duct injury, or infections. Conclusion: The Hiraga's epicanthoplasty incision could give sufficient operative field to reattach the MCT in traumatic telecanthus patients. And the oblique transnasal wiring technique is effective for the Asians who have flat nose and exophthalmic eye. The authors conclude that this technique could be a simple, safe and scarless method to correct traumatic telecanthus.