• Bulletin of the Korean Chemical Society
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• v.33 no.11
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• pp.3676-3680
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• 2012

An Epstein-Barr virus (EBV)-based plasmid contains the EBV nuclear antigen 1 (EBNA1) gene and EBV replication origin (oriP) sequence. Since EBNA1 (the only EBV-encoded protein) is combined with oriP, it is replicated simultaneously with chromosomal DNA in human, primate, and canine cells and is faithfully segregated at a stable copy number upon cell division. Consequently, it can be used to stably express gene inserts over a prolonged time in target cells. We have previously shown that the polyamidoamine (PAMAM) dendrimer can be surface-modified with L-arginine. Arginine is present at a high frequency in the transactivator of transcription (Tat) sequences of human immunodeficiency virus (HIV). It presents high membrane permeability and permits effective transfer of DNA inside the cells. In this study, we constructed two kinds of recombinant DNA by inserting the luciferase gene and enhanced green fluorescence protein (eGFP) gene as reporter genes into the pCEP4 plasmid vector. We measured dynamic light scattering (DLS) and zeta potential after preparing PAMAM-based cationic polymer/EBV-based plasmid complexes. We performed transfection of HEK 293 cell lines with the polyplexes, and monitored luciferase activity and green fluorescence protein (GFP) expression. Our results show that PAMAM-based cationic polymer/EBV plasmid complexes provide enhanced and sustained gene expression.

• Computers and Concrete
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• v.27 no.4
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• pp.319-332
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• 2021

The performance of gene expression programming (GEP) in predicting the compressive strength of bacteria-incorporated geopolymer concrete (GPC) was examined in this study. Ground-granulated blast-furnace slag (GGBS), new bacterial strains, fly ash (FA), silica fume (SF), metakaolin (MK), and manufactured sand were used as ingredients in the concrete mixture. For the geopolymer preparation, an 8 M sodium hydroxide (NaOH) solution was used, and the ambient curing temperature (28℃) was maintained for all mixtures. The ratio of sodium silicate (Na2SiO3) to NaOH was 2.33, and the ratio of alkaline liquid to binder was 0.35. Based on experimental data collected from the literature, an evolutionary-based algorithm (GEP) was proposed to develop new predictive models for estimating the compressive strength of GPC containing bacteria. Data were classified into training and testing sets to obtain a closed-form solution using GEP. Independent variables for the model were the constituent materials of GPC, such as FA, MK, SF, and Bacillus bacteria. A total of six GEP formulations were developed for predicting the compressive strength of bacteria-incorporated GPC obtained at 1, 3, 7, 28, 56, and 90 days of curing. 80% and 20% of the data were used for training and testing the models, respectively. R2 values in the range of 0.9747 and 0.9950 (including train and test dataset) were obtained for the concrete samples, which showed that GEP can be used to predict the compressive strength of GPC containing bacteria with minimal error. Moreover, the GEP models were in good agreement with the experimental datasets and were robust and reliable. The models developed could serve as a tool for concrete constructors using geopolymers within the framework of this research.

• Coupled systems mechanics
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• v.12 no.3
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• pp.199-220
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• 2023

This article presents a new analytical model to study the effect of porosity on the shear correction factors (SCFs) of functionally graded porous beams (FGPB). For this analysis, uneven and logarithmic-uneven porosity functions are adopted to be distributed through the thickness of the FGP beams. Critical to the application of this theory is a determination of the correction factor, which appears as a coefficient in the expression for the transverse shear stress resultant; to compensate for the assumption that the shear strain is uniform through the depth of the cross-section. Using the energy equivalence principle, a general expression is derived from the static SCFs in FGPB. The resulting expression is consistent with the variationally derived results of Reissner's analysis when the latter are reduced from the two-dimensional case (plate) to the one-dimensional one (beam). A convenient algebraic form of the solution is presented and new study cases are given to illustrate the applicability of the present formulation. Numerical results are presented to illustrate the effect of the porosity distribution on the (SCFs) for various FGPBs. Further, the law of changing the mechanical properties of FG beams without porosity and the SCFare numerically validated by comparison with some available results.

• Structural Engineering and Mechanics
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• v.89 no.1
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• pp.1-11
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• 2024

This article introduces a novel analytical model for examining the impact of porosity on shear correction factors (SCFs) in functionally graded porous beams (FGPB). The study employs uneven and logarithmic-uneven modified porosity-dependent power-law functions, which are distributed throughout the thickness of the FGP beams. Additionally, a modified exponential-power law function is used to estimate the effective mechanical properties of functionally graded porous beams. The correction factor plays a crucial role in this analysis as it appears as a coefficient in the expression for the transverse shear stress resultant. It compensatesfor the assumption that the shear strain is uniform across the depth of the cross-section. By applying the energy equivalence principle, a general expression for static SCFs in FGPBs is derived. The resulting expression aligns with the findings obtained from Reissner's analysis, particularly when transitioning from the two-dimensional case (plate) to the one-dimensional case (beam). The article presents a convenient algebraic form of the solution and provides new case studies to demonstrate the practicality of the proposed formulation. Numerical results are also presented to illustrate the influence of porosity distribution on SCFs for different types of FGPBs. Furthermore, the article validates the numerical consistency of the mechanical property changesin FG beams without porosity and the SCF by comparing them with available results.

• The Journal of the Korea Contents Association
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• v.22 no.10
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• pp.195-212
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• 2022

Based on the kinetic typography principle and the structure features of Chinese characters, this study took the Chinese'24 solar terms' theme dynamic poster as the research object, explored the visual expression of dynamic Chinese characters, and tried to summarize the visual expression law of Chinese characters in dynamic poster design. It can be found that, there could be 6 different types of Chinese character expressions in the 24 solar terms poster design. Among them, 'Drawing' design method has the meaning of text structure and form expression, and 'Assembling' design method has the meaning of text stroke and texture association, also, 'Forming' design method bring its meaning through stroke deformation, 'Transforming' design method conveys the content through text disintegration, 'Replacing' design method mainly bring the meaning through simulation, while 'Rotation' design method always express through visual three-dimensional and space. Finally, the findings could not only provide analytical logic and methods for the expression of Chinese characters in dynamic poster design, but also fill the lack of formative research on dynamic Chinese characters, which hopefully provide basic information for the research related to dynamic Chinese character structure, as well as the dynamic poster designers.

• Structural Engineering and Mechanics
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• v.52 no.2
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• pp.323-349
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• 2014

In this paper, linear buckling analysis of a curved sandwich beam with a flexible core is investigated. Derivation of equations for face sheets is accomplished via the classical theory of curved beam, whereas for the flexible core, the elasticity equations in polar coordinates are implemented. Employing the von-Karman type geometrical non-linearity in strain-displacement relations, nonlinear governing equations are resulted. Linear pre-buckling analysis is performed neglecting the rotation effects in pre-buckling state. Stability equations are concluded based on the adjacent equilibrium criterion. Considering the movable simply supported type of boundary conditions, suitable trigonometric solutions are adopted which satisfy the assumed edge conditions. The critical uniform load of the beam is obtained as a closed-form expression. Numerical results cover the effects of various parameters on the critical buckling load of the curved beam. It is shown that, face thickness, core thickness, core module, fiber angle of faces, stacking sequence of faces and openin angle of the beam all affect greatly on the buckling pressure of the beam and its buckled shape.

• International Journal of Internet, Broadcasting and Communication
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• v.12 no.4
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• pp.18-25
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• 2020

In the fifth generation (5G) and beyond 5G (B5G) mobile communication, non-orthogonal multiple access (NOMA) has attracted great attention due to higher spectral efficiency and massive connectivity. We investigate the impacts of the channel estimation errors on the bit-error rate (BER) of NOMA, especially with the single-user decoding (SUD) receiver, which does not perform successive interference cancellation (SIC), in contrast to the conventional SIC NOMA scheme. First, an analytical expression of the BER for SUD NOMA with channel estimation errors is derived. Then, it is demonstrated that the BER performance degrades severely up to the power allocation less than about 20%. Additionally, we show that for the fixed power allocation of 10% in such power allocation range, the signal-to-noise (SNR) loss owing to channel estimation errors is about 5 dB. As a consequence, the channel estimation error should be considered for the design of the SUD NOMA scheme.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2000.04b
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• pp.43-50
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• 2000

In the first-order shear deformation laminated beam theory (FSDT), the Kirchhoff hypothesis is relaxed such that the transverse normals do not remain perpendicular to the midsurface after deformation. Bending behavior of laminated composite thin-walled beams with singly- and doubly-symmetric open sections under uniformly distributed and concentrated loads is analyzed by the Timoshenko-type thin-walled beam theory. A closed-form expression for the shear correction factor of I-shaped composite laminated section is obtained. Numerical examples are presented to compare present analytical solutions by FSDT with the finite element solutions obtained by using three dimensional model. The effects of lamination of scheme and length-to-height ratio on the shear deformation of laminated composite beams with various boundary conditions are studied.

• Journal of Korean Society for Atmospheric Environment
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• v.21 no.5
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• pp.547-555
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• 2005

In this study, an analytical expression for aerosol mass transfer at spherical collector in the low Knudsen number region was obtained. Happel's zero shear stress cell model was extended in the low Knudsen number region and the result was compared with numerical solution results. The zero vorticity model based on the Kuwabara's cell model was also extended in the low Knudsen number region and compared with Happel's results. The results showed that both analytic and numerical solution agree very well with each other in low Knudsen number region. Happel's zero shear stress model also agrees with Kuwabara's zero vorticity model without significant loss of accuracy. The obtained solution converges to the original solution of Lee et al. (1999) when Knudsen number approaches to zero. Subsequently, this study derived most general type of analytic solution for aerosol mass transfer of spherical collector including the finite Knudsen number region.

• Proceedings of the Optical Society of Korea Conference
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• 1990.02a
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• pp.162-166
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• 1990

Photoinduced anisotropy (PIA) in amorphous As2S3 (a-As2S3 ) thin film, deposited by vacuum evaporation, is investigated. PIA is induced by linearly polarized Ar+ laser beam (λ=514.5nm) and probed by weak Ar+ laser (λ=514.5nm) and He-Ne laser (λ=632.8nm) beam through the crossed analyzer. Keeping pump beam intensity constantly, rotation of pump beam polarization direction induces reorientation phenomina of anisotropic axis. Introducing directional factor into simplified 3-level system, which is used to analyze photodarkening phenomina, an analytical expression of PIA is derived. Temporal behavior of PIAand its reorientation phenomina are investigated andcompared with theory. In the experiment pump beam intensity is 100mW/$\textrm{cm}^2$ and thickness of a-As2S3 thin film is 3${\mu}{\textrm}{m}$. In those condition, time constant of photoinduced anisotropy obtained by method of least square curve fitting is 4.0$\times$10-2sec-1. The time constant of PIA we obtained is larger than that of photodarkening, 2.8$\times$10-2sec-1.