• 한국산림과학회지
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• 제82권2호
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• pp.176-187
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• 1993

천연활엽수림(天然闊葉樹林) 군집(群集)에서 수종(樹種)의 천이계열(遷移系列)상의 위치를 해석(解析)하기 위하여 84개 활엽수(闊葉樹) 교목(喬木) 및 관목(灌木) 수종들의 생태형태학적(生態形態學的) 특성 분석을 바탕으로 극성상지수(極盛相指數)를 추정(推定)하였다. 생태형태학적(生態形態學的) 특성은 천이(遷移) 단계와 관계있다고 판단한 19가지를 선정하였으며, 각 수종별로 특성마다 극성상으로 갈수록 증가하는 2-4단계의 표준화된 점수를 부여하고 총점에 대한 합계점수의 백분율로써 극성상지수(極盛相指數)로 삼았다. 연구 대상 수종 중에서 서어나무의 지수(指數)가 83.3으로 최고치를 기록하였고 사시나무의 지수(指數)가 18.8로써 최저치로 추정되었으며, 전체 지수(指數)의 평균(平均)은 54.2로 산출되었다. 70이상의 지수(指數)값을 나타낸 수종은 9개, 40이상 70미만의 지수(指數)값을 나타낸 수종은 58개, 그리고 40미만의 지수(指數)값을 나타낸 수종은 17개로 집계(集計)되어, 천이(遷移) 중반단계의 삼림이 갖는 다양한 자원(資源) 혹은 생태적(生態的) 지위(地位)(niche)를 이용하는 수종(樹種)의 수가 압도적으로 많음을 알 수 있다. 주성분분석(主成分分析)을 통하여 각 수종이 광선흡수(光線吸收), 번식(繁殖), 그리고 목재(木材) 성질(性質) 등의 요인(要因)에 따르는 위치를 3차원(次元) 좌표(座標)상에 ordination하였고, cluster분석(分析)을 통하여 유사(類似)한 특징을 가진 4가지 수종군(樹種群)을 분류(分類)하였다. 과(科)별로 극성상지수(極盛相指數) 범위를 파악한 결과, 자작나무과(科)와 단풍나무과(科)에 속하는 수종들의 지수(指數) 범위가 넓었고, 버드나무과의 수종들은 선구수종의 전형적인 특성을 나타내었으나, 특별히 극성상(極盛相) 생활형(生活形)의 수종군(樹種群)을 갖는 과(科)는 없었다.

• 대한본초학회지
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• 제21권3호
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• pp.91-95
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• 2006

Objectives : This study was performed to determine if an antler could be identified as one of the Cervus species by phylogenetic analysis, which was used to assess genetic authentication. Methods : The DNAs of an antler were extracted, amplified by PCR, and sequenced. The DNAs of an antler were identified by Phylogenetic analysis. Phylogenetic analysis was made using MEGA software (Molecular Evolutionary Genetics Analysis, 3.1) Results : By phylogenetic analysis an antler was identified as Cervus elaphus nelsoni not as Cervus elaphus sibericus. This work showed that authentication can efficiently be performed by phylogenetic analysis. Conclusion : These results suggest that phylogenetic analysis might be able to provide the authentication of Cervus species.

• 농업과학연구
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• 제23권1호
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• pp.99-107
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• 1996

Eighteen nuclear probes were used to examine RFLP(restriction fragment length polymorphism) between six species of Artemisia spp. of Korea. Total DNA from six different species of Artemisia was separately cut with three restrict enzymes. The PstI enzyme was showed to reduce the variation of polymorphisms than the other two enzymes(EcoRl and BamHI). The genetic variation of polymorphism was similar between the Dhewegiki-ssug and Cham-ssug. RAPD analysis was applied to the same six species of Artemisia spp. in order to assess the degree of DNA polymorphism within the Artemisia genus. Six species of Artemisia were evaluated for variation using a set of 11 random 10-mer primers. Nine out of the eleven primers revealed scorable polymorphisms between six species of Artemisia spp. Genetic distances between each of the species were calculated and cluster analysis was used to generate a dendrogram showing phylogenetic relationships between them This result indicates that molecular markers will be more usable in intraspecific study of Artemisia spp. than isoenzyme markers.

• 한국환경복원기술학회지
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• 제25권5호
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• pp.59-76
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• 2022

This study was conducted to investigated woody species community classification and understory vegetation characteristics of Abies nephrolepis forest in Mt. Gyebang and Mt. Odae designated as Protected Area for Forest Genetic Resource Conservation and National Park. Vascular plants were surveyed as following; 55 family, 148 species, 3 sub species, 14 variety, 2 form 167 taxa. As an analysis of the species estimator of Jackknife 1, 29 survey plots could be representative of vegetation in study site. Results of the woody species, 3 communities were divided by cluster analysis(A=Abies nephrolepis-Tilia amurensis, B=Betula ermanii-Abies nephrolepis, C=Quercus mongolica-Abies nephrolepis). Using MRPP-test, there were significant differences between the communities indicating analysis of the appropriate group classification(p<0.01). The basal area and altitude were analyzed by negative relationship for NMS ordination. By analyzing plexus diagram of woody species, Acer pseudosieboldianum is strong associated with Quercus mongolica and Rhododendron schlippenbachii. Magnolia sieboldii is strong associated with Acer pictum subsp. mono etc.

• Animal cells and systems
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• 제13권3호
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• pp.331-337
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• 2009

Twenty-eight Anopheles species has been so-far identified in Iran, while only 8 species was proved as malaria vector. In this study, we principally examined the cuticular hydrocarbon (CHC) potency in identification of Iranian vectors of malaria and then differentiation of vector and non-vector species of Anopheles. Seven species of malaria vectors and the non-vector species, Anopheles claviger were collected throughout Iran. Female extracts were made out of every five conspecific specimens by surface immersion in pure n-hexane. Each sample was injected into a FID-GC instrument along with the known concentrations of standards. CHC profiles of the eight Anopheles species indicated no qualitative difference. The average mass of each eluted CHC were compared using Repeated ANOVA and Mann-Whitney tests. Results confirmed a significant difference in mass of each single CHC at a specific retention time (RT). Statistical comparison of CHC mass in An. sacharovi, An. stephensi, An. culicifacies and An. fluviatilis at RT 39.6 indicated significant differences (P<0.05) among these species. Analysis of CHC mass of An. dthali, An. superpictus & An. sacharovi at RT 28.5, An. stephensi & An. sacharovi at RT 30.7 and An. sacharovi & An. claviger at RT 30.6 similarly indicated significant differences (P<0.05). An. sacharovi could be distinguished from other species, which showed only trace, by integratable peaks at retention times of 29.7, 31 and 32.6. Similarly, An. claviger could be distinguished from the other species with a trace peak at RT 30.6. In order to separate An. stephensi from the five other species, the integratable peak at RT 30.7 was used. An. dthali could be identified at RT 26.2 by an integratable peak v.s. the trace peaks of other species. An. superpictus had indicator peaks at RTs 27.4 & 28.5 v.s. trace peaks of other species. An. maculipennis with its trace peak at RT 39.6 could be easily differentiated from An. fluviatilis & An. culicifacies. This study proved that all of the examined species of Anopheles could be well identified based on their quantitative differences in CHCs, except for An. fluviatilis & An. culicifacies for which no CHC indicator peak was detected.

• 수산경영론집
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• 제52권3호
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• pp.27-38
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• 2021

This study is aimed to figure out the productivity and variability of cage-aquaculture changes. According to the analysis, the productivity of major fish species has been increasing, except mullet. Although the regional productivity has decreased in the last two years, it has been on the rise considering as a whole. Gyeongsangnam-do showed the highest level of productivity by region. Productivity by species was also higher than other regions in the cases of rock fish, mullet and sea bream followed by productivity of Chungcheongnam-do. The production of marine cage-culture in Jeollanam-do is the second largest in Korea in value/weight while its productivity is lower than that of Chungcheongnam-do. When it comes to comparison by region, Gyeongsangnam-do shows the lowest productivity variation. And Jeollanam-do shows the second-lowest variation in productivity that is only about half of that of Chungcheongnam-do province. Thus, it is found that Jeollanam-do region has an advantage in management stability while its productivity is low. On the other hand, productivity by species was also analyzed. Gyeongsangnam-do has the highest productivity by species for rock fish, mullet and sea bream whereas rock bream productivity is the highest in Jeollanam-do. Therefore, it probably needs to reflect these results when choosing regional-focused incubation fish species.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.55-55
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• 2022

Corylopsis Siebold & Zucc. (Hamamelidaceae) is widely used for horticultural plant and comprise ca. 25 species in East Asia (1 species in Korea; 4 species in Japan; 20 species in China). Previous revisions have gone from 7 to more than 30 species, causing confusion in the nursery industry and public gardens. Due to morphological similarity within Corylopsis, molecular research is needed to distinguish it. In this study, the chloroplast genome of C. gotoana and C. pauciflora distributed in Japan was completed by using NGS (Next-Generation Sequencing) technique. The genome size of C. gotoana and C. pauciflora were 159,434 bp (large single-copy (LSC): 88,164 bp; small single-copy (SSC): 18,702 bp; inverted repeat regions (IRs): 26,284 bp) and 159,363 bp (LSC: 88,097 bp; SSC: 18,700 bp; IRs: 26,283 bp), respectively. In addition, we investigated the repeats, SNPs, and indels, and that could be used as DNA markers. Phylogenetic analysis demonstrated that C. pauciflora was sister to C. gotoana and C. spicata. The genus Corylopsis is a monophyletic group and Loropetalum is closely related to Corylopsis. The results of our study will provide the basic data necessary for the analysis of the species identification markers and genetic diversity within the genus Corylopsis in the future.

• Journal of Species Research
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• 제9권3호
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• pp.221-232
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• 2020

Selaginella vaginata is a common montane species with broad distribution in China and the Himalaya region, and several species that are morphologically similar to S. vaginata are distributed in Asia. The taxonomic revision of S. vaginata and related species was performed by morphological comparison of leaves, strobili, and spores, and phylogenetic analysis. Based on these results, a new species, S. subvaginata, sp. nov., has been identified. Morphologically, S. subvaginata has intermediate form between S. vaginata and S. repanda, which differs mainly in its main stem being erect, dorsal leaves long-ciliolate on inner margin and outer margin denticulate or with 2-4 cilia at base (long-ciliolate on both inner and outer margins in S. vaginata, denticulate on both inner and outer margins in S. repanda), and acroscopic base of ventral leaves long ciliolate (sparsely long ciliolate in S. vaginata, short ciliolate to denticulate in S. repanda). Moreover, phylogenetic analysis using three chloroplast markers(rbcL, atpI, and psbA) revealed that S. subvaginata is a distinct species among the anisosporophyllous species clade in Selaginellaceae.

• 한국조경학회지
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• 제33권2호
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• pp.48-59
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• 2005

The purpose of this study was to examine the ecological characteristics of herbaceous communities by systematic and scientific analysis of their structure and diversity in the Midongsan Arboretum and to offer raw data for a long-tenn monitoring study. The importance value and diversity index of species appearing in twenty plots from July to September, 2004 were analyzed and a management plan for these communities is presented. Vascular plants were represented by 60 taxa of 23 families, 51 genera, 50 species and 10 varieties. Based on the results of importance value analysis, the most dominant species was Artemisia princeps var. orientalis, followed by Setaria viridis and Erigeron canadensis. The diversity index analysis showed that plot no. 5 had the highest H' and H'_{max}$(2.0135 and 2.6391). It's species composition was comparatively more diverse and it's structure more stable than other plots. Artemisia montana and Dactylis glomerata showed the highest correlation between species. Because herbaceous communities are important biological habitats and provide important function in environmental conservation, it is important to properly preserve these communities. At the same time, in order to preserve genetic resources and improve spatial function, it may be necessary to consider removing herbaceous communities in certain areas. In the future, the relations between physicochemical soil properties and herbaceous communities should be examined and community movement should be studied.

• 대한본초학회지
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• 제21권2호
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• pp.129-133
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• 2006

Objectives : Cervus elaphus species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Cenvus elaphus species could be identified by sequencing analysis and to verify Basic Local Alignment Search Tool (BLAST) search, which was used to assess genetic identification. Methods : The DNAs of Cervus elaphus species were extracted, amplified by PCR, and sequenced. The DNAs of Cervus species were identified by BLAST search in website. Results : By BLAST search one of Cervus elaphus species was identified as Cervus elaphussibericus but the other was identified as Cervus elaphus nelsoni. This work showed that identification can efficiently be performed by BLAST search. Conclusion : These results suggest that sequencing following BLAST search might be able to provide the identification of Cervus elaphus species.