• 상하수도학회지
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• 제21권4호
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• pp.467-475
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• 2007

This study was conducted to examine temperature effects on hydrogen production in anaerobic fermentation. 18 batch reactors were operated at mesophilic ($35^{\circ}C$) and thermophilic conditions ($55^{\circ}C$) to achieve maximum hydrogen production in anaerobic fermentation. Optimum hydrogen production conditions were also investigated at each temperature. Different trends were observed regarding pH effects on hydrogen production. This effect was not significant for mesophilic fermentation ($35^{\circ}C$). In this case, pH may not drop to interfere hydrogen production during the test. However, hydrogen production decreased without pH control for thermophilic condition ($55^{\circ}C$). Effects of heat treatment were observed for both fermentation process. Hydrogen production with heat treatment was higher than hydrogen production without heat treatment for both fermentation processes. The amount of produced hydrogen for each substrate concentration with temperature changes showed that more hydrogen was produced at $35^{\circ}C$ than at $55^{\circ}C$.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 제17회 워크샵 및 추계학술대회
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• pp.299-306
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• 2005

Anaerobic fermentation of food waste (FW) and waste activated sludge (WAS) for hydrogen production was performed in CSTR (Continuous Stirred tank reactor) under various HRTs and volumetric mixing ratio (V/V) of two substrates, FW and WAS. The specific hydrogen production potential of FW was higher than that of WAS. However, pH drop in the CSTR for hydrogen production from FW was higher than that from WAS. The maintenance of desired pH during fermentative hydrogen production is regarded as the most important operation parameter for the stable hydrogen production. Therefore, when the potential of hydrogen production from FW and better buffer capacity of WAS, the proper mixture of FW and WAS for fermentative hydrogen production were considered as a useful complementary substrate. The maximum yield of specific hydrogen production, 140 mL/g VSS, was found at HRT of 2 day and the volumetric mixing ratio of 20:80 (WAS : FW). The spatial distribution of hydrogen producing bacteria was observed in anaerobic fermentative reactor using fluorescent in situ hybridization (FISH) method.

• 신재생에너지
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• 제5권4호
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• pp.80-85
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• 2009

The effects of various organic wastes on anaerobic fermentative hydrogen production were studied using enriched mixed microflora in batch tests. Rotten fruit, corn powder and organic wastewater enriched with sulfate (up to 1,000 mg/L) were used for experiments. Maximum hydrogen production (547.1 mL) was observed from rotten apple with initial substrate concentration of 132.2 g COD/L. The experimental result on sulfate enriched organic wastewater indicated that hydrogen production is not adversely influenced by relatively high sulfate concentration. Residual sulfate content remained at 96-98 % after 75 hours of reaction, which showed that no major sulfate reduction was occurred at pH 5.3-5.5 in the reactor. The volatile fatty acid (VFA) fractions produced during the reaction was in the order of butyrate > acetate > propionate in all experiments. The results of this study would be useful for controlling the conditions on fermentative hydrogen production using different feedstocks.

• 한국수소및신에너지학회논문집
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• 제15권3호
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• pp.187-193
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• 2004

In this study, Anaerobic sewage sludge in a batch reactor operation at $35^\circ{C}$ was used as the seed to investigate the effect of pretreatments of waste activated sludge and to evaluate its hydrogen production potential by anaerobic fermentation. Various pretreatments including physical, chemical and biological means were conducted to utilize for substrate. As a result, SCODcr of alkali and mechanical treatment was 15 and 12 times enhanced, compared with a supernatant of activated sludge. And SCODcr was 2 time increase after re-treatment with biological hydrolysis. Those were shown that sequential hybridized treatment of sludge by chemical & biological methods to conform hydrogen production potential in bath experiments. When buffer solution was added to the activated sludge, hydrogen production potential increased as compare with no addition. Combination of alkali and mechanical treatment was higher in hydrogen production potential than other treatments.

• 한국수소및신에너지학회논문집
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• 제19권2호
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• pp.145-155
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• 2008

This paper deals with an economic evaluation of hydrogen production by fermentation. We evaluate the economic feasibility of domestic hydrogen production by fermentation utilizing glucose and waste water sludge in terms of hydrogen production prices. In addition, we make some sensitivity analysis of hydrogen prices by changing the values of input factors such as the price of glucose, the capital cost of the hydrogen production system, and the hydrogen production yields. The estimated hydrogen prices of the two-step dark-light hydrogen production by fermentation utilizing glucose was $5,347won/kgH_2$, and the single-step hydrogen production by anaerobic fermentation utilizing waste water sludge was $4,255won/kgH_2$, respectively. It is expected that the hydrogen production price by anaerobic fermentation can be reduced if we produce methane or hydrogen utilizing by-products such as alcohols and organic acids, or the government imposes some legal regulations on the treatment of waste water sludge.

• 상하수도학회지
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• 제21권5호
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• pp.641-647
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• 2007

This study aims to find out optimum condition for hydrogen production and organic removal when treating food and swine wastewater together. For this purpose, various batch tests were conducted by changing mixture ratio from 6:4 (food wastewater:swine wastewater) to 1:9 without pretreatment process. For hydrogen production through anaerobic fermentation, the mixture ratios of R-1 (6:4), R-2 (5:5) and R-6 (1:9) were out of pH range appropriate for hydrogen production and mixture ratios of R-3 (4:6), R-4 (3:7), and R-5(2:8) showed appropriate hydrogen production where their pH ranges were 5.1~5.5. Especially in case of R-3, it consistently maintained appropriate pH range for hydrogen production for 72hr and produced maximum hydrogen. The characteristics of hydrogen production and cumulative hydrogen production according to each mixture ratio showed that R-1, R-2 and R-6 did not produce any hydrogen, and maximum hydrogen productions of R-3, R-4 and R-5 were 593ml, 419ml and 90ml, respectively. Total cumulative hydrogen productions of R-3, R-4 and R-5 were 1690ml, 425ml and 96ml, respectively. Based on previous results, it was concluded that, the most appropriate mixture ratio of food wastewater and swine wastewate rwas 4:6 (R-3). The experiment for COD removal rate to evaluate organic removal efficiency revealed that R-3, R-4 and R-5 showed high removal efficiencies during the highest hydrogen production amount and the highest efficiency was 41% with R-3.

• 상하수도학회지
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• 제22권3호
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• pp.299-306
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• 2008

This study has been conducted to derive the bio-energy, hydrogen and methane production, from mixture of food wastewater and swine wastewater, the high strength organic wastewater and to increase effluent quality. To overcome this limitation in one-phase anaerobic process, two-phase anaerobic process combining hydrogen fermenter and methane fermenter was applied. In this system $2,323ml\;H_2/L$ was produced daily from Run II where 500 ml of heattreated sludge in methane fermenter was injected, and methane produced from methane fermenter did not show big difference regardless of the amount of returning sludge at each Run. It was concluded that the two-phase anaerobic process was the appropriat process to produce hydrogen and methane simultaneously and stably. Influent $TCOD_{Cr}$ to two-phase anaerobic process showed the range of 132~145 g/L(average 140 g/L), and effluent $TCOD_{Cr}$ range was 25~40 g/L(average 32 g/L), and organic removal efficiency showed 71~82%(average 76.3%).

• 대한토목학회논문집
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• 제26권6B호
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• pp.683-687
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• 2006

본 연구에서는 혐기성 소화에서 aeration이 수소생성에 미치는 영향에 대해 살펴보았다. 혐기성 소화슬러지를 0, 1, 3, 6, 12, 24 시간 동안 aeration 실시 후 glucose(20 g/L)를 기질로 이용하여 batch test를 실시하였다. Aeration 시간이 길어질수록 메탄가스가 감소하고, 수소가스가 증가하였으며, 6시간 동안 aeration을 실시한 반응조에서 가장 높은 수소 생성율(570 ml/L)을 나타내었다. 연속운전의 경우 aerated reactor는 메탄가스의 생성 없이 수소가 지속적으로 발생하였으며, non-aerated reactor의 경우 낮은 pH와 짧은 HRT만으로는 메탄 생성균의 활성을 완전히 저해할 수 없었다. 그러나 미생물관점에서의 보다 명확한 규명을 위해 향후 연구가 추가적으로 진행되어야 하며, 현장 적용성을 고려한 aeration 처리의 최적조건 도출도 이루어져야 할 것이다.

• 한국습지학회지
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• 제9권1호
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• pp.123-131
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• 2007

Effect of nitrogen-load condition on hydrogen ($H_2$) production and bacterial community in a continuous anaerobic hydrogen fermentation were investigated. The slight $H_2$ production on extremely low nitrogen-load condition (C/N ratio: 180) at the start-up period. The highest $H_2$ production was obtained when the C/N ratio was 36, the $H_2$ production yield ($mol-H_2/mol-glucose$) reached to 1.7, and it was indicated that Clostridium pasteurianum mainly contributed to the $H_2$ production. The $H_2$ production was decreased on both the lower (C/N: 72) and higher (C/N: 18) nitrogen-load conditions. The excess nitrogen-load was not always suitable for the hydrogen production. The fluctuation of $H_2$ production seemed to be caused by a change in the bacterial community according to the nitrogen-load condition, while a recovery of $H_2$ productivity was possible by a control of nitrogen-load condition through the bacterial community change. When the nitrogen-load condition was not suitable for hydrogen production, the lactic acid concentration was increased and also lactic acid bacteria were definitely detected, which suggested that the competition between hydrogen fermentator and lactic acid producer was occurred. These results demonstrated that the nitrogen-load condition affect on the $H_2$ productivity through the change of bacterial community in anaerobic hydrogen fermentation.

• 유기물자원화
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• 제11권1호
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• pp.70-76
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• 2003

소화슬러지를 열처리한 혼합 배양 미생물을 식종균으로, 자당을 기질로 사용하는 혐기성 수소 생산 공정을 운전하면서 pH 조절, 반복 열처리, 기질 농도 변화 등을 통해 지속적인 수소 생산 방법을 고찰하였다. 유입 기질의 농도가 5g COD/L인 경우 운전 초기에는 $0.5mole\;H_2/mole\;hexose$ 이상의 수소가 발생하지만 9일 이상 지속되지 못하였다. 이러한 현상의 원인은 수소 생성균이 공정 내에서 고농도로 존재하지 못하기 때문인 것으로 판단되며 pH를 5.3으로 유지하는 것만으로는 극복될 수 없었다. 반복 열처리를 적용할 경우 별도의 식종균 재주입 없이 효율이 감소된 수소 생성 공정을 원상태로 복구할 수 있음을 확인할 수 있었으나, 수소 생성 효율이 시간에 따라 감소하므로 열처리를 자주 해야 하는 문제가 발생했다. 유입 기질의 농도가 30g COD/L인 경우에는 24일간 지속적인 수소 생성이 가능하였으며, CSTR의 경우 $1.0-1.4mole\;H_2/mole\;hexose$, ASBR의 경우에는 $0.2-0.3mole\;H_2/mole\;hexose$의 생성 효율을 보였다. 수소 생성 시 유출수 내 용존성 유기물의 90% 이상은 유기산이었으며 그 중 n-butyrate가 가장 많은 양을 차지하였다.