• BMB Reports
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• 제45권6호
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• pp.342-347
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• 2012

Plant mitochondria possess alternative respiratory pathways mediated by the type II NAD(P)H dehydrogenases and alternative oxidases. Here, E3 SUMO ligase was shown to regulate alternative respiratory pathways and to participate in the maintenance of carbon and nitrogen balance in Arabidopsis. The transcript abundance of the type II NAD(P)H dehydrogenases NDA2 and NDB2 and alternative oxidases AOX1a and AOX1d genes was low in siz1-2 mutants compared to that in wild-type. The addition of nitrate or ammonium resulted in a decrease or an increase in the expression of the same gene families, respectively, in both wild-type and siz1-2 mutants. The amount of free sugar (glucose, fructose and sucrose) was lower in siz1-2 mutants than that in wild-type. These results indicate that low nitrate reductase activity due to the AtSIZ1 mutation is correlated with an overall decrease in alternative respiration and with a low carbohydrate content to maintain the carbon to nitrogen ratio in siz1-2 mutants.

• 설비공학논문집
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• 제16권11호
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• pp.1060-1067
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• 2004

To apply an analysis method of life cycle cost when assessing economics of equipment system, we should basically set up preconditions such as useful life, price escalation rate, interest rate, etc. as well as consider a calculation algorism of source energy and heat source system, which is a complex process for life cycle costing. For this reason, equipment designers tend to plan heat source systems, without a thorough investigation on economics of alternative systems at the pre-design phase. In this process, architectural designers should adopt a proper heat source system, which is one of the most important factors for planning an appropriate architectural design, through a discussion with equipment designers in a short time. In order to offer an evaluation method for equipment designers to analyze economics of an alternative heat source system easily at the pre-design phase, this research would define the simplified economics, evaluation method through analysis of existing papers for economics evaluation, and examine validity through comparison of simplified method values ($LCC_{EC}$) and life cycle costing values ($LCC_{15}$) for six alternative heat source systems.

• Human Ecology Research
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• 제57권2호
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• pp.271-280
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• 2019

This study collects and analyzes data on educational philosophy and method for domestic and overseas alternative schools. This information is then applied to educational purpose and curriculum for a home economics curriculum that develops unique characteristics for Korean alternative education.This study collected and analyzed the characteristics of educational content and educational elements from two renowned alternative schools: Waldorf School in Germany and Summer Hill in the United Kingdom. The research results were as follows. First, it is necessary to create new word instead of using the term 'alternative education'. Second, among characteristics of Korean home economics curriculum, 'practical problem-solving ability', 'independent living ability', and 'relation forming ability' should be utilized to create a school with an independent curriculum and to establish a unique brand value. Third, time investment on instructor education should be made to cultivate competent and charming instructors as the center of a school. Lastly, school education should manage student's life at school and after graduation. The school and instructor should provide alternative education while thinking they are responsible for 50 years of a student's future life. This study helps show that alternative education is not perceived as a superficial and generalized education and that Korean alternative education can find similar characteristic to those of overseas cases.

• Molecules and Cells
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• 제37권1호
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• pp.81-87
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• 2014

Chronic pressure-overload cardiac hypertrophy is associated with an increased risk of morbidity/mortality, largely due to maladaptive remodeling and dilatation that progresses to dilated cardiomyopathy. Alternative splicing is an important biological mechanism that generates proteomic complexity and diversity. The recent development of next-generation RNA sequencing has improved our understanding of the qualitative signatures associated with alternative splicing in various biological conditions. However, the role of alternative splicing in cardiac hypertrophy is yet unknown. The present study employed RNA-Seq and a bioinformatic approach to detect the RNA splicing regulatory elements involved in alternative splicing during pressure-overload cardiac hypertrophy. We found GC-rich exonic motifs that regulate intron retention in 5' UTRs and AT-rich exonic motifs that are involved in exclusion of the AT-rich elements that cause mRNA instability in 3' UTRs. We also identified motifs in the intronic regions involved in exon exclusion and inclusion, which predicted splicing factors that bind to these motifs. We found, through Western blotting, that the expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, were significantly altered during cardiac hypertrophy. Collectively, the present results suggest that chronic pressure-overload hypertrophy is closely associated with distinct alternative splicing due to altered expression of splicing factors.

• Asian-Australasian Journal of Animal Sciences
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• 제30권10호
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• pp.1471-1477
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• 2017

Objective: Since athletic performance is a most importance trait in horses, most research focused on physiological and physical studies of horse athletic abilities. In contrast, the molecular analysis as well as the regulatory pathway studies remain insufficient for evaluation and prediction of horse athletic abilities. In our previous study, we identified AXL receptor tyrosine kinase (AXL) gene which was expressed as alternative spliced isoforms in skeletal muscle during exercise. In the present study, we validated two AXL alternative splicing transcripts (named as AXLa for long form and AXLb for short form) in equine skeletal muscle to gain insight(s) into the role of each alternative transcript during exercise. Methods: We validated two isoforms of AXL transcripts in horse tissues by reverse transcriptase polymerase chain reaction (RT-PCR), and then cloned the transcripts to confirm the alternative locus and its sequences. Additionally, we examined the expression patterns of AXLa and AXLb transcripts in horse tissues by quantitative RT-PCR (qRT-PCR). Results: Both of AXLa and AXLb transcripts were expressed in horse skeletal muscle and the expression levels were significantly increased after exercise. The sequencing analysis showed that there was an alternative splicing event at exon 11 between AXLa and AXLb transcripts. 3-dimentional (3D) prediction of the alternative protein structures revealed that the structural distance of the connective region between fibronectin type 3 (FN3) and immunoglobin (Ig) domain was different between two alternative isoforms. Conclusion: It is assumed that the expression patterns of AXLa and AXLb transcripts would be involved in regulation of exercise-induced stress in horse muscle possibly through an $NF-{\kappa}B$ signaling pathway. Further study is necessary to uncover biological function(s) and significance of the alternative splicing isoforms in race horse skeletal muscle.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.376-380
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• 2015

Life-Cycle Cost Analysis (LCCA) for highway projects is known as an effective analytical technique that uses economic principles to evaluate long-term alternative investment options, especially for comparing the values of alternative pavement design structures and construction strategies. In the Unites States, the 2012 Moving Ahead for Progress in the 21^st Century Act (MAP-21) amended the United States Code to mandate that the United States Government Accountability Office (GOA) conducts a study of the best practices for calculating life-cycle costs and benefits for the federally funded highway projects in 2013. The RealCost 2.5CA program was developed and adapted as an official LCCA tool to comply with regulatory requirements for California state highway projects in 2013. Utilization of this California-customized LCCA software helps Caltrans to achieve substantial economic benefits (agency cost and road user cost savings) for highway projects. Proper implementation of LCCA for roadway construction and rehabilitation would deliver noticeable savings of agency's roadway maintenance cost especially in developing counties where financial difficulties exist.

• 한국정보시스템학회지:정보시스템연구
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• 제29권1호
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• pp.75-91
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• 2020

Purpose Retrofit, defined to be addition of new technologies or features to the old system to increase efficiency or to abate GHG emissions, is considered as an important alternative for the old coal-fired power plant. The purpose of this study is to propose mathematical method to model multiple alternative retrofit in Generation Capacity Expansion Planning(GCEP) problem, and to get insight to the retrofit patterns from realistic case studies. Design/methodology/approach This study made a multi-alternative retrofit GECP model by adopting some new variables and equations to the existing GECP model. Added variables and equations are to ensure the retrofit feature that the life time of retrofitted plant is the remaining life time of the old power plant. We formulated such that multiple retrofit alternatives are simultaneously compared and the best retrofit alternative can be selected. And we found that old approach to model retrofit has a problem that old plant with long remaining life time is retrofitted earlier than the one with short remaining life time, fixed the problem by some constraints with some binary variables. Therefore, the proposed model is formulated into a mixed binary programming problem, and coded and run using the GAMS/cplex. Findings According to the empirical analysis result, we found that approach to model the multiple alternative retrofit proposed in this study is comparing simultaneously multiple retrofit alternatives and select the best retrofit satisfying the retrofit features related to the life time. And we found that retrofit order problem is cleared. In addition, the model is expected to be very useful in evaluating and developing the national policies concerning coal-fired power plant retrofit.

• Journal of Ecology and Environment
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• 제41권10호
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• pp.281-289
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• 2017

Background: In this study, the growth and reproductive response of seedlings, grown in plastic pots with sand, to moisture and nutrients were analyzed in order to study the environmental conditions required to create an alternative habitat for Epilobium hirsutum L., an endangered plant. Results: Vegetative and reproductive growths of Epilobium hirsutum L. are accelerated with increase in moisture and organic matter content in the soil. Among vegetative organs, the number of runners related to asexual reproduction was the highest when the moisture content was over 25% and nutrient content between 7 and 14% in the soil. But the number of flowers related to flowering responses, among reproductive organs, was the highest when the moisture content was maintained at 75% and when nutrient content was 21% in the soil. The number of seeds, related to sexual reproduction, was the highest when the moisture content was over 25% and nutrient content between 14 and 21%. Conclusions: The study results show that a place with high moisture and nutrient content in the soil is advantageous to asexual and sexual reproduction of Epilobium hirsutum L. Therefore, we must serve periodically nutrient and seeds to sustain population in in situ conservation. Furthermore, it is advisable to create in riverside where abundant nutrient content have, making alternative habitat of Epilobium hirsutum L. Also, we must find species that have high contribution degree index through vegetation survey.

• 가정과삶의질연구
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• 제21권2호
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• pp.75-85
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• 2003

The purpose of this study was to investigate the attitudes toward divorce of young men and women. The data were collected by questionnaire from 425 respondents who resided in Sunchon. Statistical methods for the data analysis were frequencies, one-way ANOVA, t-test. The data analysis revealed that the young men and women accepted divorce as an alternative to unhappy marriage and there were significant gender differences in the attitudes toward divorce. Women accepted divorce more positively as an alternative than men in every conditions. Men accepted divorce remarkably as an alternative to unfaithful debauchery of spouse, but women accepted divorce extremely well as an alternative to wrongdoing of spouse. Most of young men and women were worried about children, but not a few of them did not perceived themselves as child rearer. This results showed that divorce will be increased and perceived differently by gender. The young men and women who had lower conservative familism, who were younger, and who had higher education responded more positively about divorce.

• 한국축산식품학회지
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• 제44권5호
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• pp.1156-1166
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• 2024

Texture is a major challenge in addressing the need to find sustainable meat alternatives, as consumers desire alternative meat to have a sensory profile like meat. In this study, the fabrication of imitated muscle fiber (IMF) is performed by introducing different kinds of protein sources, with an effective bottom-up technique- wet spinning. Herein, the protein sources (pea protein isolate, wheat protein, and myofibrillar paste) were combined with sodium alginate to stimulate the bonding with the coagulation solution for fabrication. It has been found that the fabrication of IMF is possible using all the protein sources, however, due to the difference in protein structure, a significant difference was observed in quality characteristics compared to conventional meat. Additionally, combination of wheat protein and pea protein isolate has given similar values as conventional meat in terms of some of the texture profiles and Warner-Bratzler shear force. In general, the optimization of protein sources for wet spinning can provides a novel way for the production of edible fiber of alternative meat.