• Title/Summary/Keyword: amylose

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Growth, Feed Utilization and Blood Metabolic Responses to Different Amylose-amylopectin Ratio Fed Diets in Tilapia (Oreochromis niloticus)

  • Chen, Meng-Yao;Ye, Ji-Dan;Yang, Wei;Wang, Kun
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.8
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    • pp.1160-1171
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    • 2013
  • A feeding trial was conducted in tilapia to determine the growth performance, nutrient digestibility, digestive enzymes, and postprandial blood metabolites in response to different dietary amylose-amylopectin ratios. Five isonitrogenous and isolipidic diets containing an equal starch level with different amylose-amylopectin ratios of 0.11 (diet 1), 0.24 (diet 2), 0.47 (diet 3), 0.76 (diet 4) and 0.98 (diet 5) were formulated using high-amylose corn starch (as the amylose source) and waxy rice (as the amylopectin source). Each diet was hand-fed to six tanks of 15 fish each, three times a day over a 6-wk period. After the growth trial, a postprandial blood metabolic test was carried out. Fish fed diet 2 exhibited the highest percent weight gain and feed efficiency and protein efficiency ratio, whereas fish fed with diet 5 showed the lowest growth and feed utilization among treatments. The digestibility for starch in fish fed diet 1 and 2 was higher than those in fish fed with other diets (p<0.05). The highest activities for protease, lipase and amylase were found in fish fed the diet 2, diet 1, and diet 1 respectively among dietary treatments, while the lowest values for these indexes were observed in fish fed the diet 3, diet 5 and diet 4, respectively. The liver glycogen concentrations in fish fed diets 4 and 5 were found higher than in fish fed other diets (p<0.05). The feeding rate, hepatosomatic index, condition factor, and plasma parmeters (glucose, triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol) did not differ across treatments. In terms of postprandial blood responses, peak blood glucose and triglycerides were lower after 3 or 6 h in the fish fed with diets 3-5 than in the fish fed diet 1, but delayed peak blood total amino acid time was observed in fish fed with the diets 1 or 2. The lowest peak values for each of the three blood metabolites were observed in fish fed diet 5. The results indicate that high-dietary amylose-amylopectin ratio could compromise growth, but help in reducing the blood glucose stress on fish caused by postprandial starch load.

Crystalline Structure of the Extrudate of High Amylose Corn Starch (압출성형 고아밀로즈 옥수수전분의 결정 구조)

  • Kim, Ji-Yong;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1024-1028
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    • 1998
  • Crystalline structure of the extrudate of high amylose corn starch was studied by X-ray diffractometer and $^{13}C\;NMR$. The X-ray diffraction crystal ratio of the extrudates (barrel temperature $100^{\circ}C$ )of high amylose corn starch slightly increased from 6.08% to 8.37% by increasing feed moisture content from 25% to 45%. But extrudates of high amylose corn starch showed similar crystal ratio on various extrusion conditions. Extrudates of high amylose corn starch (feed moisture content 20%, barrel temp $140^{\circ}C$) showed more enlarged crystal structure than that of non-extrudates. The perpendicular distance of crystal increased by extrusion. Crystal ratio was changed from $6.3{\sim}8.3%$ to $4.5{\sim}5.8%$ during storage at $4^{\circ}C$. Starch configuration was examined with $^{13}C\;NMR$. Double helical content was measured by $^{13}C\;NMR$ method. The highest double helical content (60%) was obtained from high amylose corn starch extrudate (barrel temp.: $100^{\circ}C$, feed moisture content 45%). Double helical contents and resistant starch (RS) yield (pancreatin) were positively correlated. However, double helical content of the extrudates was not changed by the storage at $4^{\circ}C$.

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Comparison of Enzyme Resistant Starches Formed during Heat-Moisture Treatment and Retrogradation of High Amylose Corn Starches (수분-열처리와 노화에 의해 고아밀로오스 옥수수전분으로부터 형성된 효소저항전분의 특성비교)

  • Kweon, Mee-Ra;Shin, Mal-Shick
    • Applied Biological Chemistry
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    • v.40 no.6
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    • pp.508-513
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    • 1997
  • Thermal characteristics and granular morphology on enzyme-resistant starches (RS) formed during heat-moisture treatment (HMT) and retrogradation were investigated in high amylose corn starches, Hylon V and Hylon VII. With each treatment, both starches showed a similar trend in the increase of RS, but RS yield of Hylon VII is higher than that of Hylon V. Specially, RS was increased remarkably by HMT. It was more than doubled from 11.4% to 26.6% for Hylon V and from 15.9% to 32.8% for Hylon VII. A small increase of RS resulted from retrogradation. HMT on starch increased gelatinization temperature, decreased enthalpy. Retrograded starch exhibited small three endothermic transitions at $94^{\circ}C$, $110^{\circ}C$ and $140^{\circ}C$ in differential scanning calorimetry (DSC) thermogram due to the remained ungelatinized starch granules, dissociation of amylose-lipid complex and melting of recrystallized amylose, respectively. Enzyme-resistant starches isolated from native and heat-moisture treated starches showed a broad endothermic transition at higher temperature than native starch, while retrograded starch exhibited a very sharp peak at ${\sim}150^{\circ}C$ due to the melting of amylose crystallites. Under microscopy, starch granules with HMT was not changed, but retrograded starches showed the aggregates of starch granules because amylose leached out during gelatinization. Iodine stained RS clearly showed the differences in enzyme hydrolysis on the native, heat-moisture treated and retrograded starches.

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The Effect of Roasted Soybean Flour Supplimentation to Jeolpyons(Korean Rice Cake) on Changing the Contents of Amino Acid, Amylose, and Minerals (볶은 콩가루 첨가가 절편의 아미노산, 아밀롯 및 무기물 함량에 미치는 영향)

  • 정해옥;정복미
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.4
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    • pp.614-617
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    • 1994
  • This study was undertaken to determine the changes in amino acids, amylose and mineral contents of Jeolpyons(Korean rice cake) supplemented with various levels of roasted soybean flour (RSF). Amino acids content of Jeolpyons increased as RSF content increased. Especially the contents of total essential amino acids of Jeolpyon supplemented with RSF(5, 10, 15 and 20%) increased 1.22 to 2.74 times greater than those of the ocntrol. Amylose, which is related to retrogradatin effect on rice products decreased about 0.9 to 4.7% by increasing the soybean flour contents. Mineral contents by jeolpyon containing RSF increased as RSF contents increased.

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Relationship Between Hot Water Solubles of Rice and Texture of Cooked Rice (쌀의 열수 가용성 물질과 밥의 텍스처와의 관계)

  • Kim, Kwan;Kang, Kil-Jin;Kim, Sung-Kon
    • Korean Journal of Food Science and Technology
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    • v.23 no.4
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    • pp.498-502
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    • 1991
  • The relationships between the textural properties of cooked rice and hot water-solubles of rice or rice flour were studied. The amounts of solubles or soluble amylose among japonica varieties were similar, but those among Tongil type varieties were different. At the same heating conditions, japonica varieties showed higher contents of solubles as well as soluble amylose than Tongil varieties. The solubles and soluble amylose from rice or rice flour showed a negative correlation with hardness but a positive correlation with adhesivess of rice cooked with optimum water.

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Physical and Structural Characteristics of Endosperm Starch of Four Local Barnyardgrass (Echinochloa crus-galli L.) Collections in Korea

  • Kim, Sang-Kuk;Lee, Hee-Dong;Ryu, Jung-Gi;Choi, Hong-Jib;Kang, Sang-Mo;Lee, In-Jung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.4
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    • pp.293-298
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    • 2011
  • Physical and structural characteristics of four local barnyardgrass flour and starch were investigated. Amylose content ranged from 6.9 to 12.5%. The BP 3563 and BP 3606 were low amylose lines, and the BP 3592 and BP 3610 showed very low amylose lines. Peak viscosities of four barnyardgrass collections BP-3563, BP-3592, BP-3606, and BP-3610 ranged 264 to 2397. All barnyard grass lines displayed an A-type X-ray diffraction pattern showing the strong diffraction peak at around $2{\theta}$ values $15^{\circ}$, $17^{\circ}$, $18^{\circ}$ and $23^{\circ}$, and a small peak at $20^{\circ}$ $2{\theta}$. Scanning electron microscopy revealed that the starch granule sizes ranged $5.5-12.5{\mu}m$ in diameter showing polygonal and rarely spherical in shape with round edges and some pores at the surface. Starch crystallinity of BP 3563, BP 3592, BP 3606, and BP 3610 was 23.9, 25.0, 24.7, and 25.5%, respectively.

Identification and purification of Wx protein involved in biosynthesis of amylose in Rice (벼에서의 아밀로즈 생합성 관련 Wx 단백질의 동정 및 분리)

  • Nahm, Baek-Hie;Kim, Jin-Ku;Choi, Hae-Choon
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.533-538
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    • 1993
  • The Wx protein, known as starch synthase or starch glucosyl transferase (E.C. 2.4.1.11), is responsible for the amylose synthesis. In an effort to explain the mechanism of amylose biosynthesis, the starch synthase known as Wx protein was identified by analyzing the various wx rice mutants with SDS-PAGE of proteins extracted from rice starch. Finally, the 66 kDa protein was purified by extracting the starch-bound protein fractions followed by Suprose 12 gel filtration chromatography.

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Influence of Water and Surfactants on Wheat Starch Gelatinization and Retrogradation (수분과 계면활성제가 밀전분의 호화와 노화에 미치는 영향)

  • Shin, Mal-Shick
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.116-121
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    • 1991
  • The effects of water contents and surfactants, sodium stearoyl-lactylate, sucrose ester and distilled monoglyceride(Dimodan) on wheat starch gelatinization and retrogradation were studied by differential scanning calorimetry. The endothermic peak patterns of starch varied with water content of starch. When water content was less than 30%, gelatinization did not occur. The onset temperature of gelatinization peak of native starch was $59{\sim}60^{\circ}C$ and that of endothermal peak of retrograded starch was $50{\sim}55^{\circ}C$. The enthalpy value of retrograded starch were greatest in the $40{\sim}50%$ water content. In the presence of surfactants, gelatinization temperatures shifted slightly to higher temperatures. DSC endothermal enthalpies of the amylose-lipid complex increased. The degree of retrogradation in starch was lower with surfactant than without surfactant, but enthalpy of amylose-lipid complex did not change during storage.

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