• Earthquakes and Structures
• /
• 제12권2호
• /
• pp.251-262
• /
• 2017

The present research intends to study the effects of the seismic soil-foundation-structure interaction (SFSI) on the dynamic response of various buildings. Two methods including direct and Cone model were studied through 3D finite element method using ABAQUS software. Cone model as an approximate method to consider the SFSI phenomenon was developed and evaluated for both high and low rise buildings. Effect of soil nonlinearity, foundation rigidity and embedment as well as friction coefficient between soil-foundation interfaces during seismic excitation are investigated. Validity and performance of both approaches are evaluated as reference graphs for Cone model and infinite boundary condition, soil nonlinearity and amplification factor for direct method. A series of calculations by DeepSoil for inverse earthquake record modification was conducted. A comparison of the two methods was carried out by root-mean-square-deviation (RMSD) tool for maximum lateral displacement and story shear forces which verifies that Cone model results have good agreement with direct method. It was concluded that Cone method is a convenient, fast and rather accurate method as an approximate way to count for soil media.

• Journal of Microbiology and Biotechnology
• /
• 제12권1호
• /
• pp.65-70
• /
• 2002

A TGF-${\beta}1$/GFP monomeric fusion protein was cloned from pPK9A and pGFP-Cl plasmid by PCR amplification. The fusion protein was expressed in a $Bac-To-Bac^{TM}$ baculovirus expression system. A 45 kDa fusion protein was purified using an Ni-NTA column with 300 mM imidazol from a cell lysate infected with recombinant viruses for 72 h post-infection. The fusion protein cross-reacted with the commercial $TGF-{\beta}1$ polyclonal Ab as well as Ab raised against a precursor, monomeric $TGF-{\beta}1$, and GFP. The binding activity of the fusion protein with a $TGF-{\beta}1$ receptor was examined. Fluorescence was observed in Mv1Lu cells, yet not in insect cells treated with the fusion protein. No fluorescence was detected in Mv1Lu cells incubated with the fusion protein treated with Ab prior to the binding reaction, or with GFP alone, thereby indicating that the binding of the fusion protein was specific to $TGF-{\beta}1$ with a receptor.

• Journal of Microbiology and Biotechnology
• /
• 제8권1호
• /
• pp.34-41
• /
• 1998

A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권7호
• /
• pp.2591-2600
• /
• 2015

Breast cancer is the most common cancer worldwide among women and the second most common cancer. Approximately 15-23% of breast cancers over-express human epidermal growth factor receptor2 (HER2), a 185-kDa transmembrane tyrosine kinase, which is mainly found at the cell surface of tumor cells. HER2-positive breast cancer, featuring amplification of HER2/neu and negative expression of ER and PR, has the three following characteristics: rapid tumor growth, lower survival rate, and better response to adjuvant therapies. Clinically, it is notable for its role in a pathogenesis that is associated with increased disease recurrence and acts as a worse prognosis. At the same time, it represents a good target for anti-cancer immunotherapy despite the prevalence of drug resistance. New treatments are a major topic of research, and a brighter future can be expected. This review discusses the role of HER2 in breast cancer, therapeutic modalities available and prognostic factors.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제11권2호
• /
• pp.154-159
• /
• 2006

Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18) was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the plant expression vector under the control of a duplicated CaMV 35S promoter. The recombinant plasmid was transformed into tobacco (Nicotiana tabacum L. cv Havana) using the Agrobacterium-mediated transformation method. The integration of the hlL-18 gene into the genome of transgenic tobacco plants was confirmed by polymerase chain reaction (PCR) amplification and its expression was observed in the suspension cells that were derived from the transgenic plant callus by using Northern blot analysis. The hlL-18 protein was detected in the extracts of the transgenic callus and in the medium of the transgenic tobacco suspension culture by using immunoblot analysis. Based upon enzyme-linked immunosorbant assay (ELISA) results, the expression level of the hlL-18 protein approximated $166{\mu}g/L$ in the suspension culture medium. Bioassay results from the induction of $interferon-{\gamma}$ from a KG-1 cell line indicated that the hlL-18 secreted into the suspension culture medium was bioactive.

• Progress in Superconductivity
• /
• 제12권1호
• /
• pp.57-61
• /
• 2010

We have developed a whole-head MEG system for basic brain research and clinical application. The sensor system consists of a 152 SQUID gradiometer array oriented and located in a suitable way to cover a whole head of the human. The system measures magnetic fields generated by neuronal currents in the brain to get information on the brain activities. For this purpose, the field sensitivity determined by the position, orientation and geometry of the pickup coil as well as amplification factor of the electronic circuits should be known precisely. However, the position and orientation of the pickup coil might be changed from the designed specifications during cool down of the dewar and it is necessary to characterize the field sensitivity. In this study, we made calibration systems to determine the actual position and orientation of the 152 pickup coils and compared the localization results of the N100m source in the auditory cortex.

• Geomechanics and Engineering
• /
• 제23권1호
• /
• pp.1-13
• /
• 2020

To give a solution for seismic stability of tunnel faces subjected to earthquake ground shakings, the pseudo-dynamic approach is originally introduced to analyze tunnel face stability in this study. In the light of the upper-bound theorem of limit analysis, an advanced three-dimensional mechanism combined with pseudo-dynamic approach is proposed. Based on this mechanism, the required support pressure on tunnel face can be obtained by equaling external work rates to the internal energy dissipation and implementing an optimization searching procedure related to time. Both time and space feature of seismic waves are properly accounted for in the proposed mechanism. For this reason, the proposed mechanism can better represent the actual influence of seismic motion and has a remarkable advantage in evaluating the effects of vertical seismic acceleration, soil amplification factor, seismic wave period and initial phase difference on tunnel face stability. Furthermore, the pseudo-dynamic approach is compared with the pseudo-static approach. The difference between them is illustrated from a new but understandable perspective. The comparison demonstrates that the pseudo-static approach is a conservative method but still could provide precise enough results as the pseudo-dynamic approach if the value of seismic wavelengths is large or the height of soil structures is small.

• 대한기관식도과학회지
• /
• 제4권2호
• /
• pp.197-204
• /
• 1998

Human papillomavirus(HPV) is epitheliotrophic virus invading the anogenital tract and the upper aerodigestive tract HRV produces a diversity of benign and maljgnant tumors. In this study, the author determined the frequency of association of human papillomavirus(HPV) and laryngeal carcinomas and investigated the significance of HRV infection of different subtypes in the tumorigenesis of laryngeal carcinoma. Laryngeal squamous cell cancinomas from 34 patients who did not have preexisting papillomas by clinical history were retrieved from formalin-fixed, paraffin-embedded blocks and analyzed for HPV. Nineteen cases were tumors of the true vocal folds, 11 were supraglottic and 4 were transglottic. HPV detection was dane using polymerase chain reaction amplification with HPV L$_1$consensus primer. HPV type was determined by the same method using HPV-6, 11 and 16,-18 type-specific E6 primers. The results were as follows : 1) HPV DNA was detected in 7 cases among the 34 patients(20.6%). According to the type of HPV DNA HPV-11 was detected in 3 cases, HPV-16 was detected in 2 cases and HPV-6 and HPV-18 were detected in 1 case, respectively. 2) These 7 HPV-positive patients were advanced cancinoma cases. From these results, we concluded that HPV was thought to be the etiological factor of laryngeal squamous cell carcinomas.

• 한국전산구조공학회:학술대회논문집
• /
• 한국전산구조공학회 2011년도 정기 학술대회
• /
• pp.743-746
• /
• 2011

본 연구에서는 유한요소해석을 이용하여 차량속도 및 긴장재의 설계에 따른 CFTA 거더의 동적거동 및 동적증폭계수를 분석하였다. 긴장재의 해석변수로서는 긴장재의 양과 긴장력을 고려하였으며 차량하중은 도로교설계기준의 DB-24 하중을 선택하였다. 차량하중은 3축-2트랙에 작용하는 등가절점하중으로 변환하여 속도에 따라 시간함수로 모델링하였다. 긴장재의 양은 외측 �� 내측 덕트의 유무에 따라 변화시켰으며 긴장력은 설계긴장력의 0%에서 100%까지 25%씩 증가시켰다. 차량속도는 40km/hr에서 100km/hr까지 20km/hr씩 증가시켰으며, 해석결과 긴장재의 긴장력 변화는 거더의 동적거동에 영향을 주지 않았으며 초기처짐에만 영향을 주었다. 긴장재의 양에 따라서는 거더의 동적거동이 다르게 나타났으며 긴장재의 양이 적을 수록 동적처짐은 증가하였다. 이를 바탕으로 거더의 동적증폭계수(DAF)를 산출하였으며, 이 결과 긴장재가 없는 경우에도 도로교표준시방서에서 정한 기준 값보다 매우 작은 거동을 보였다.

• 한국지진공학회논문집
• /
• 제20권7_spc호
• /
• pp.537-543
• /
• 2016

In low to moderate seismic regions, there are limited earthquake ground motion data recorded from past earthquakes. In this regard, the Gyeongju earthquake (M=5.8)occurred on September 12, 2016 produces valuable information on ground motions. Ground motions were recorded at various recording stations located widely in Korean peninsula. Without actual recoded ground motions, it is impossible to make a ground motion prediction model. In this study, a point source model is constructed to accurately simulate ground motions recorded at different stations located on different soil conditions during the Gyeongju earthquake. Using the model, ground motions are generated at all grid locations of Korean peninsula. Each grid size has $0.1^{\circ}(latitude){\times}0.1^{\circ}(longitude)$. Then a contour hazard map is constructed using the peak ground acceleration of the simulated ground motions.