• 대한토목학회논문집
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• 제30권3A호
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• pp.211-219
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• 2010

고속철도교량의 설계개념은 정적하중에 따른 충격계수를 고려하여 기존 교량 구조물의 강성을 증가시키기 위한 방안을 적용하고 있으며, 전반적인 구조설계 과정은 선진 외국기술에 의존하고 있는 실정이다. 그러나 고속철도(Korea Train eXpress: KTX)의 긴 연장(380 m)과 고속(300 km/h) 주행은 공진현상에 상당한 영향을 미치기 때문에 고속철도교량의 동적증폭계수(DAF) 및 동적성능 평가는 상세한 검토가 필수적으로 수행되어야 할 것이다. 따라서 이 연구에서는 전형적인 PSC 박스 거더 고속철도교량을 대상으로 동적성능을 효율적으로 검토하고자 하며, 합리적인 구조설계를 위한 기초자료를 제시하고자 한다. 이를 위해, 기존문헌을 토대로 KTX의 하중선도를 고려하여 정적해석을 수행하였다. 또한 다양한 해석변수를 고려하여 KTX의 이동하중을 시계열하중으로 변환하였으며, 변환된 시계열하중을 이용하여 시간이력해석을 합리적으로 평가하였다. 이때, 시계열하중을 산정하기 위한 변수는 KTX의 하중재하 절점간격, 시간증분 및 속도변화를 고려하였다. FE해석 결과를 바탕으로 PSC 박스 거더 고속철도교량의 동적성능을 체계적으로 검토하였으며, 국내외 관련규정에 따라 구조안전성을 정량적으로 평가하였다.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.379-384
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• 2012

Lhx8 (LIM homeobox 8) gene encodes a LIM homeodomain transcriptional regulator that is preferentially expressed in germ cells and critical for mammalian folliculogenesis. However, Lhx8 DNA binding sequences are not characterized yet. We aimed to identify and characterize a cis-acting sequence of germ-cell specific transcriptional factor, Lhx8. To identify Lhx8 DNA binding element, Cyclic Amplification of Sequence Target (CAST) Analysis was performed. Electrophoretic Mobility Shift Assay (EMSA) was processed for the binding specificity of Lhx8. Luciferase assay was for the transcriptional activity of Lhx8 through identified DNA binding site. We identified a putative cis-acting sequence, TGATTG as Lhx8 DNA binding element (LBE). In addition, Lhx8 binds to the LBE with high affinity and augments transcriptional activity of luciferase reporter driven by artificial promoter containing the Lhx8 binding element. These findings indicate that Lhx8 directly regulates the transcription of genes containing Lhx8 binding element in oocytes during early folliculogenesis.

• Structural Engineering and Mechanics
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• 제69권3호
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• pp.347-359
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• 2019

This paper aims to evaluate the behavior of Dorim-Goh bridge in Seoul, Korea, under static and dynamic loads effects by ambient trucks. The prestressed concrete (PSC) girders and reinforcement concrete (RC) slab of the bridge are evaluated and assessed. A short period monitoring system is designed which comprises displacement, strain and accelerometer sensors to measure the bridge performance under static and dynamic trucks loads. The statistical analysis is used to assess the static behavior of the bridge and the wavelet analysis and probabilistic using Weibull distribution are used to evaluate the frequency and reliability of the dynamic behavior of the bridge. The results show that the bridge is safe under static and dynamic loading cases. In the static evaluation, the measured neutral axis position of the girders is deviated within 5% from its theoretical position. The dynamic amplification factor of the bridge girder and slab are lower than the design value of that factor. The Weibull shape parameters are decreased, it which means that the bridge performance decreases under dynamic loads effect. The bridge girder and slab's frequencies are higher than the design values and constant under different truck speeds.

• Earthquakes and Structures
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• 제23권6호
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• pp.517-526
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• 2022

Previous studies have shown that pile-soil interactions have significant influences on the isolation efficiency of an isolated structure. However, most of the existing tests were carried out using a 1-g shaking table, which cannot reproduce the soil stresses resulting in distortion of the simulated pile-soil interactions. In this study, a centrifuge shaking table modelling of the seismic responses of a friction pendulum bearing isolated structure with a pile foundation under earthquakes were conducted. The pile foundation structure was designed and constructed with a scale factor of 1:100. Two layers of the foundation soil, i.e., the bottom layer was made of plaster and the upper layer was normal soil, were carefully prepared to meet the similitude requirement. Seismic responses, including strains, displacement, acceleration, and soil pressure were collected. The settlement of the soil, sliding of the isolator, dynamic amplification factor and bending moment of the piles were analysed to reveal the influence of the soil structure interaction on the seismic performance of the structure. It is found that the soil rotates significantly under earthquake motions and the peak rotation is about 0.021 degree under 24.0 g motions. The isolator cannot return to the initial position after the tests because of the unrecoverable deformation of the soil and the friction between the curved surface of the slider and the concave plate.

• 한국항공우주학회지
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• 제41권1호
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• pp.17-24
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• 2013

본 논문에서는 복합재료의 미시적 파손모드를 고려하는 복합재 파손예측 프로그램을 개발하였다. 개발된 프로그램의 검증을 위하여 원공이 있는 복합재 적층판 시편의 인장시험 및 정적 파손해석을 수행하였다. 먼저 적층각도별 복합재 시편에 대한 인장시험을 통하여 논문에 사용된 재료에 대한 SIFT 허용치를 산출하였고, 미시역학적 모델인 RVE에 대한 유한요소 해석을 통하여 변형률 증폭계수를 결정하였다. 또한 원공이 있는 복합재 적층판 시편에 대한 인장시험을 수행하고, 실험을 통해 얻어진 파손하중 결과를 바탕으로 유한요소 모델에 대하여 정적 파손해석을 수행하였다. 마지막으로 실험결과를 바탕으로 예측된 파손지수 결과를 평가함으로써 개발된 프로그램의 효용성을 검증하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4623-4627
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• 2014

Background: The epidermal growth factor receptor (EGFR) plays a vital role in the activation and inactivation of receptor tyrosine kinases. Mutations in exons 19 and 21 of EGFR are commonly found to be associated with non small cell lung carcinoma and triple negative breast cancer, enhancing sensitivity to EGFR targeting chemotherapeutic agents. Since amplification and prolonged activation of EGFR molecules have been identified in oral squamous cell carcinomas (OSCC), we investigated whether OSCCs carried mutations in exons 19 and 21 of EGFR to their incidence. Materials and Methods: Tumor chromosomal DNA isolated from forty surgically excised oral squamous cell carcinoma tissues was subjected to PCR amplification with intronic primers flanking exons 19 and 21 of the EGFR gene. The PCR amplicons were subsequently subjected to direct sequencing to elucidate the mutation status. Results: Data analysis of the EGFR exon 19 and 21 coding sequences did not show any mutations in the forty OSCC samples that were analyzed. Conclusions: To the best of our knowledge, this is the first study to have investigated the genetic status of exons 19 and 21 of EGFR in Indian OSCCs and identified that mutation in EGFR exon 19 and 21 may not contribute towards their genesis. The absence of mutations also indicates that oral cancerous lesions may not be as sensitive as other cancers to chemotherapeutic agents targeting EGFR.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.161-167
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• 2004

Silkworms sex determination drew high attention from researchers. Sex chromosomes on the silkworm are of ZW type for females and ZZ type for males. Chromosome W plays an important role in sex determination. Although several molecular linkage maps have been constructed for silkworm, very few markers are discovered on the W chromosome. In order to look for molecular markers and to further locate the Fern gene on chromosome W, we used genomic DNA from both female and male larvae of a silkworm strain named 937 as PCR templates for RAPD amplification with 200 arbitrary 10-mer primers. The amplification results showed three female-specific bands, namely ${OPG-07_496}, {OPC-15_1,660} and {OPE-18_1,279}$. Further verification, however, revealed no band from OPG-07 and OPC-15 in either sex in the strain 798, but OPE-18 provided female-specific band in the strains Suluan7 and C108, and absent in both males and strain 798. This indicates that the bands from ${OPG-07_496} and {OPC-15_1,660}$ are probably female-specific in strain 937, and the band from OPE-18 was probably amplified from a common segment shared by most strains. The genomic DNAs from OPG-07 and OPC-15 were cloned and sequenced. Sequence analysis showed that the DNAs from OPG-07 and OPC-15 have high identities with the retrotransposable elements, and DNA from OPC-15 contains a portion of sequence which probably encodes an eukaryotic translation initiation factor 4E binding protein (eIF4EBP).

• 천문학회지
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• 제45권5호
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• pp.127-138
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• 2012

We explore how wave-particle interactions affect diffusive shock acceleration (DSA) at astrophysical shocks by performing time-dependent kinetic simulations, in which phenomenological models for magnetic field amplification (MFA), Alfv$\acute{e}$nic drift, thermal leakage injection, Bohm-like diffusion, and a free escape boundary are implemented. If the injection fraction of cosmic-ray (CR) particles is ${\xi}$ > $2{\times}10^{-4}$, for the shock parameters relevant for young supernova remnants, DSA is efficient enough to develop a significant shock precursor due to CR feedback, and magnetic field can be amplified up to a factor of 20 via CR streaming instability in the upstream region. If scattering centers drift with Alfv$\acute{e}$n speed in the amplified magnetic field, the CR energy spectrum can be steepened significantly and the acceleration efficiency is reduced. Nonlinear DSA with self-consistent MFA and Alfv$\acute{e}$nic drift predicts that the postshock CR pressure saturates roughly at ~10 % of the shock ram pressure for strong shocks with a sonic Mach number ranging $20{\leq}M_s{\leq}100$. Since the amplified magnetic field follows the flow modification in the precursor, the low energy end of the particle spectrum is softened much more than the high energy end. As a result, the concave curvature in the energy spectra does not disappear entirely even with the help of Alfv$\acute{e}$nic drift. For shocks with a moderate Alfv$\acute{e}$n Mach number ($M_A$ < 10), the accelerated CR spectrum can become as steep as $E^{-2.1}$ - $E^{-2.3}$, which is more consistent with the observed CR spectrum and gamma-ray photon spectrum of several young supernova remnants.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1015-1018
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• 2016

Background: Overexpression or amplification of human epidermal growth factor receptor-2 (HER2) is associated with grade of malignancy and a poor prognosis in breast cancer (BC). The aim of this study was to evaluate of value of HER2 as a prognostic marker, and to analyze associations with common histopathological parameters in BC cases. Materials and Methods: Between of 2007 to 2014, 260 patients with BC referred to Oncology Clinic provided cancer tissue samples which underwent immunohistochemistry (IHC) for markers. ER and PR positivity was defined as ${\geq}10%$ positive tumor cells with nuclear staining. HER2-positive was defined as either HER2 gene amplification by fluorescent in situ hybridization (FISH) or scored as 3+ by IHC. For HER2 (2+), FISH was performed to determine HER2 positivity. Results: The mean age at diagnosis for the patients with HER2-negative was significantly higher than in HER2-positive cases. Also, there were significant correlations between histological grade, nuclear grade, lymph node metastasis, tumor size, ER status, PR status, p53 overexpression and Ki-67 index with HER2 expression. HER2-negative lesions were of higher grade and more likely to be ER-negative, PR-negative, p53-positive, lymph node metastasis, with a tumor size<2cm and also $Ki-67{\geq}20%$ as compared to the HER2-positive group. Conclusions: Contrary to the results of other studies, HER2-positive tumors in our study had a lower Ki-67 index and were p53-positive. Also, Ki-67 proliferation index ${\geq}20%$ in more studies was associated with p53-positive.Therefore, tumors which are HER2-positive and have a Ki-$67{\geq}20%$ had a more aggressive behavior compared to HER2-positive and Ki-67<20% lesions.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7885-7889
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• 2014

Background: Activating mutations of epidermal growth factor receptor (EGFR) could predict response to tyrosine kinase inhibitor (TKI) treatment in patients with non-small cell lung cancer (NSCLC). However, the detection of EGFR mutation is frequently challenging in clinical practice for the lack of tumor tissue. The aim of this study was to investigate the feasibility of performing EGFR mutation testing on various types of liquid-based cytology (LBC) samples. Materials and Methods: A total of 434 liquid-based cytology samples were collected from March 2010 and November 2013. Among them, 101 with diagnosis of lung adenocarcinoma had paired surgically resected specimens. The ADx Amplification Refractory Mutation System (ADx-ARMS) was used to determine EGFR mutation status both in LBC and resected samples. Results: All liquid-based cytology samples were adequate for EGFR mutation analysis. The mutation rate was 50.5% in the 434 NSCLC patients with LBC samples and the incidence rates of EGFR mutation were consistent among different specimens. We also detected EGFR positives in 52.5% (53/101) patients with paired histologic specimens. The concordance rate of EGFR mutation between LBC samples and paired histologic specimens was 92.1%. Conclusions: Our results suggest that liquid-based cytology samples are highly reliable for EGFR mutation testing in patients with NSCLC.