• Title/Summary/Keyword: ammonium effect

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Seasonal Nutrient Analysis of Paddy Soils in Korean Type of Large Scale Environment-friendly Agricultural District (한국형 광역친환경 논 농업단지 토양의 시기별 양분 분석)

  • Choi, Hyun-Sug;Jung, Seok-Kyu
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.373-386
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    • 2017
  • The study was conducted to investigate seasonal nutrient dynamics in rice-cultivated soils collected from farmhouses of three large-scale environment-friendly agricultural districts (LEAD), Jangheung, Suncheon, and Okcheon in which environmental-friendly agriculture has been exemplarily practiced in Korea. Among three districts, Crop- livestock cycling organic farming system had been introduced only in Jangheung. pH and EC of farmhouse soils of three LEADs were ranged between 5.5 - 6.7 and $0.4-1.0dS\;m^{-1}$ from March to September, respectively. T-N was observed to be high on the farmhouse soil in Suncheon and K was observed to be lowest on farmhouse soil in Okcheon. Concentration of $NH_4-N$ in soil was observed to be highest on June, in particular on the farmhouse in Jangheung, but rapidly decreased due to the loss of fertilizer applied in Spring. Yield and harvest index were the highest on the farmhouse in Okcheon in which total annual gross production $ha^{-1}$ was nearly three time higher than those of other two farmhouses. Farmhouse soil of Okcheon was maintained the highest seasonal nutrient balance due to the high input of fertilizer. It was estimated that K balance in the farmhouse soil in Suncheon dropped to $-60kg\;ha^{-1}$ on September, and it might have some effect on the less rice productivity due to K deficiency. Farmhouse soil in Jangheung was maintained low seasonal balance of T-N and P but showed the highest N use efficiency in the rice grain. Based on above-mentioned results, we think Jangheung farmhouse can be recommended as a model farmhouse of LEADs.

Characterization of a Fibrinolytic Enzyme from Pickled Anchovy (멸치젓갈유래의 혈전용해호소에 대한 특성)

  • Yang Woong-Suk;Lim Hak-Seob;Chung Kyung Tae;Kim Young-Hee;Huh Man Kyu;Choi Byung Tae;Choi Yung Hyun;Jeong Yong Kee
    • Journal of Life Science
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    • v.15 no.3 s.70
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    • pp.434-438
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    • 2005
  • In the previous study, we isolated a myulchikinase (MK), which has fibrinolytic activity and cytotoxicity to the tumor cell line, from myl- chi-jeot-gal. In this study, the effect of NaCl concentration, metallic ions, pH, temperature, and plasminogen on the activity of MK was analysed. The MK activity was maintained at least $80\%$ activity up to $30\%$ NaCl, which indicates that the enzyme may be halotolerant. The optimal pH and temperature were 8 and $40^{\circ}C$, respectively. The fibrinolytic activity of MK was completely inhibited with 0.5 mM $Hg^{2+}$ and inhibited to $50^{\circ}C$ with 1 mM $Cu^{2+}\;and\;Zn^{2+}$. The MK showed strong activity in plasminogen- rich fibrin plate but not in plasminogen-free fibrin plate. The result indicates that the MK may be a plasminogen activator type fibrinolytic enzyme.

A study on the Manufacture and Application of UV-Cured Anti-stain Coating Compounds for PVC Tile (PVC 바닥상재용 광경화형 내오염 코팅액의 제조 및 응용에 관한 연구)

  • Yoon, Hyun-Jung;Ha, Jin-Wook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.5
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    • pp.1741-1746
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    • 2010
  • This study is on development of UV-cured anti-stain coating compounds which have more improved anti-stain function to prevent a surface of PVC tile from stain. To make an anti-stain coating composition, water soluble antistatic agent made from ammonium(IV) salt, antistatic agents for acrylic and polyurethane were used. Their contents varied from 5 to 20wt% against quantities of resin in coating composition. After coating PVC tiles using bar-coating method that can adjust a thickness, we estimated surface properties of coated layer such as anti-stain, electric resistance, adhesive power, thickness of coating, and so on. Results showed that a coating composition added 15wt% of water soluble antistatic agent and coated with No.12 bar-coater had the optimum surface property in electric resistance($3.24{\times}10^9{\Omega}/cm^2$), anti-stain(ink Test, Dust Test) and adhesive power. We could also find electric resistance and anti-stain effect were improved as antistatic agent content increased. However, excessive addition of antistatic agents(over 20wt%) caused the migration.

Effect of the Whole-layer Application of Slow-release Fertilizer on Growth and Yield of Rice (완효성비료의 전층시비가 벼 생육과 수량에 미치는 영향)

  • 박경배
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.6
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    • pp.499-505
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    • 1992
  • This experiment was carried out to find out the optimum application method of slow-release fertilizer under different soil textures, clay loam(Deogpyeong series), sandy loam (Gangseo series) in the southern region(Milyang, Kyeongnam) of Korea. The fertilizers used were Chosun slow-release fertilizer(silicate latex coated fertilizer: N-P$_2$O$_{5}$-K$_2$O=18-12-13) and Meister 15 (thermoplastic resin coated fertilizer : N-P$_2$O$_{5}$-K$_2$O=14-14-14). The two whole basal application methods such as side band placement at transplanting time of rice plant(cv.Donghaebyeo) and incorporation with soil as basal were tested. The released amount of ammonium nitrogen from the soils for 4 days submerged was 95ppm in sandy loam and 60ppm in clay loam. The greenish degree of rice leaf was higher at the whole plow layer placement method than the others. The nitrogen efficiencies of the fertilizers were Meister 15 > convention > Chosun in order and between the application methods were similiar. The growth status was better at the whole plow layer placement application method of Meister 15 regardless of soils and seedling ages. The ripening ratio was increased at slow-release fertilizer application, and between application methods, whole plow layer placement was lower because of lodging damage. The yield in clay loam soil showed a significant difference between the ferilizers, but there were slight differences between the application methods and fertilizers in sandy loam soil. As a result, the slow-release fertilizer, Meister 15, applied at the whole layer showed a good rice growth and seemed a plausible fertilizing method.

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Comparison of Water, Nitrogen Uptake and Use Efficiency Treated with Silica and N Application Forms (규산과 질소형태별 처리에 따른 벼의 수분 및 질소흡수와 이용효율 비교)

  • Choi, Kyung-Jin;Lee, Jung-Il;Chung, Nam-Jin;Yang, Won-Ha;Lee, Chung-Keun;Oh, Se-Kwan;Kim, Je-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.3
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    • pp.220-225
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    • 2013
  • This experiment was carried out to elucidate the effects of silicate and different application forms of N on the uptake of water and N by rice plants. Three rice cultivars, Ilpum, Anda and M202, were grown under the hydroponics in a phytotron. One-hundred ppm silica was applied for silicate treatment. For nitrogen application forms were 100% $NH_4$ and $NH_4+NO_3$ in 2:1 ratio were applied. Silica treatment, compared to silica free, was very effective on the nitrogen uptake and dry weight increase of rice plants. Although silica application demonstrated no significant effect on the amount of water uptake, it improved increased water and nitrogen use efficiency. Therefore, sufficient application of silicate in paddy field will be useful for the growth of rice plants and water saving.

Optimization of solid phase extraction and simultaneous determination of trace anions in concentrated hydrofluoric acid by ion chromatography (불산 중 극미량 음이온 분석을 위한 고상 추출법 및 이온크로마토그래프를 이용한 동시분석법 확립)

  • Yoon, Suk-Hwan;Jo, Dong-ho;Kim, Hyun-Ji;Shin, Ho-Sang
    • Analytical Science and Technology
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    • v.29 no.5
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    • pp.219-224
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    • 2016
  • 불산 중 극미량 음이온의 고상추출과 이온크로마토그래프를 이용한 고감도 분석법이 개발되었다. 불산 중 불소이온이 고상에 의해 제거하였고 이어서 음이온 (F, CH3COO, Cl, Br, NO3, PO43−, SO42−)들이 이온크로마토그래프를 이용하여 연속적으로 분리하였다. 고상 추출법에 영향을 주는 각 인자들 (흡착제의 선택, 시료의 부피 및 pH, 용출 용액과 용출용액의 부피)을 결정하였으며 그 결과 흡착제로서 Oasis WAX 컬럼이 가장 우수하였고 1.0 mL의 시료부피, 용출용액으로 50 mM 초산암모늄염 5 mL가 분리능에서 가장 우수하였다. 개발한 방법에 의한 음이온 (Cl, Br, NO3, PO43−, SO42−)들의 방법검출한계는 25 % 불산용액 (w/w) 중에 0.04~0.30 µg/L의 범위를 보였고 정밀도는 20.0와 40.0 µg/L의 농도에서 5 % 이내를 보였다. 한 제조회사에 의한 25 % 불산 중 음이온의 4.2에서 47.5 µg/L의 범위로 모두 검출되었다. 이 방법은 시험절차가 간단하고, 재현성 및 감도가 좋아서 반도체회사에서 불산 중 음이온 불순물을 정도 관리하는데 매우 유용한 방법이 될 것으로 판단된다.

Fish Growth Experiment in a Green water Recirculating System (순환 Green Water 사육장치 내에서의 어류의 성장실험)

  • KIM In-Bae;LEE Sook-Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.233-238
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    • 1981
  • A recirculating water system without filter bed was used for a high density experimental rearing of common carp, Cyprinus carpio, during the summer of 1981. Of the water system, 3 tanks were used for stocking each tank with 10,000, 20,000 and 30,000 common carp fingerlings which averaged 0.6 g. The water in the system was maintained in dark green colour by heavy growth of phytoplankton during the most period of the experiment. In this experiment, the best daily growth rate, $3.7\%$, was obtained from the 10,000 fish group followed by $2.8\%\;and\;3.0\%$ from the 20,000 and 30,000 fish groups respectively. Thus there was no significant difference between the latter two groups. Especially, notably high concentration of total ammonia, $5\sim7$ ppm ammonia-N, in the culture water appeared during the most experimental period, but this did not seem to have affected feeding and growth of the fish when the water was kept at heavy bloom. It was also obvious that the outbreak of columnaris disease was effectively depressed in this green water recirculating system compared to that in clarified recirculating system. One tank ($7m^2$) of this system was stocked with 446 common carp of intermediate size averaging 352.2 g and after 40 day rearing they grew to 486.3 g in average with a daily growth rate of $0.8\%$. This result was a little inferior to that reared in a tank of the clarified water system as control which showed $1.0\%$ daily growth rate. Therefore, before an initiation of the commercial production of fish in this method, further studies concerning the amount of ammonium with its effect on the fish under rearing in this system and the columnaris disease problem should be carried out.

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Assessment for Effect of Water Environment by Addition of Improvement Agents on Sediments (저질 개선제의 주입에 의한 수 환경에 미치는 영향 평가)

  • Kim Woo-Hang;Kim Do-Hee
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.10 no.1 s.20
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    • pp.69-73
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    • 2004
  • Control if Sediment is very important in prawn farm due to the eruption of toxic materials such as unionized $H_{2}S,\;NH_{3}\;and\;NO_3$. In this study, column test was conducted with filter media such as activated carbon, zeolite, oyster shell and iron chloride to evaluate the reduction of toxicity from sediment. ammonia-N($NH_3$) was effectively removed by Zeolite and oyster shell. It was indicated that ammonium ion($NH_4^+$) was removed by ion exchange of zeolite. And the ammonia in the column of oyster shell was existed as the form of $NH_4^+$, which is not toxic for prawn because oyster shell was stably kept at $8{\sim}9g$ of pH. Therefore, some of ammonia($NH_4^+$) was removed by oyster shell. Hydrogen sulfide and COD were effectively removed by adsorption of activated carbon and a partial removal of hydrogen sulfide was accomplished by Oyster shell. Phosphorous was removed by activated carbon, oyster shell and iron chloride. In prawn farm, the concentration of ammonia was increased with increase of pH by algae photosynthesis in the column of activated carbon, zeolite and iron chloride, but it was revealed that pH was stably kept in the column of oyster shell.

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Study of the Molecular Reorientation in Ammonium Sulfate by Neutron Scattering

  • Kim, Huhn-Jun
    • Nuclear Engineering and Technology
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    • v.4 no.4
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    • pp.306-321
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    • 1972
  • Molecular reorientation in (NH$_4$)$_2$SO$_4$, has been studied by cold neutron scattering. For T=300$^{\circ}$K data, the isolated quasielastic spectra and form-factors at various scattering angles are compared with four reorientational models based on SKOLD theory. Front these, it is concluded that the NH$_4$ions are performing either 3-fold four axes or 2-fold three axes reorientation with $\tau$$_{c}$=2.0$\times$10$^{-11}$ sec. The temperature dependence of f. is studied over 100$^{\circ}$K-413$^{\circ}$K and for the high-temperature phase, the widths of composite spectra are compared with the results from NMR relaxation measurements. All the results have shown that the neutron scattering method is capable of giving detalis of the reorientational modes in solids and therefore some discussions are given on the application of this method. A study of the form-factor is applied for NH$_4$I (Phase I) by comparing the measurement with the calculation based on free rotation approximation and proposed a reorientation model of NH$_4$ ions in the octahedral potential cage with $\tau$$_{c}$$\leq$10$^{-12}$ sec. Also a brief theoretical prediction for the effect of reorientational motions on the inelastic spectrum is discussed.sed.

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Purification and Characterization of the Bacteriocin Produced by Lactococcus sp. KD 28 Isolated from Kimchi (김치에서 분리한 Lactococcus lactis가 생산하는 박테리오신의 정제 및 특성)

  • Lee, Ji-Young;Choi, Nack-Shick;Chun, Sung-Sik;Moon, Ja-Young;Kang, Dae-Ook
    • Journal of Life Science
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    • v.25 no.2
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    • pp.180-188
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    • 2015
  • The bacterial strain isolated from Kimchi showed antibacterial activity against Micrococcus luteus IAM 1056. The selected strain was identified as Lactococcus lactis by 16S rRNA nucleotide sequence analysis and named as Lactococcus sp. KD 28. The treatment of culture supernatant with proteinase K removed antibacterial activity, indicating its proteinaceous nature, a bacteriocin. This bacteriocin was sensitive to hydrolytic enzymes such as ${\alpha}$-chymotrypsion, trypsin, proteinase K, lipase, ${\alpha}$-amylase and subtilisin A. The bacteriocin was highly thermostable and resistant to heating at $80^{\circ}C$ for up to an hour but 50 % of the total activity was remained at $100^{\circ}C$ for 30 min. The pH range from 2.0 to 8.0 had no effect on bacteriocin activity and it was not affected by solvents such as acetonitrile, isopropanol, methanol, chloroform and acetone up to 50% concentration. The bacteriocin showed antibacterial activity against M. luteus IAM 1056, Lactobacillus delbrueckii subsp. lactis KCTC 1058, Enterococcus faecium KCTC 3095, Bacillus cereus KCTC 1013, B. subtilis KCTC 1023, Listeria ivanovii subsp. ivanovii KCTC 3444, Staphylococcus aureus subsp. aureus KCTC 1916, B. megaterium KCTC 1098 and B. sphaericus KCTC 1184. The bacteriocin was purified through ammonium sulfate concentration, SP-Sepharose chromatography and RP-HPLC. The molecular weight was estimated to be about 3.4 kDa by tricine-SDS-PAGE analysis.