• 한국막학회:학술대회논문집
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• 한국막학회 2003년도 The 4th Korea-Italy Workshop
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• pp.61-64
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• 2003

Optical resolution of a-amino acid (tryptophan and tyrosine) optical isomers was achieved by a pressure driven membrane separation process, using self-supporting crosslinked membranes base on polysaccharide with different swelling indices that ranged from 100 to 70%. The membranes prepared by casting and drying the polymer solution containing 5wt% acetic acid on an acryl plate followed by crosslinking with glutaraldehyde were characterized using such analytical methods as FTIR and swelling index measurements. On the way of separating the optical isomers, several experimental factors such as the concentration of the feed solutions, operating pressure and temperature, and degree of crosslinking of the membranes have been studied. When the chitosan membranes with 70% of swelling index were used , almost complete optical resolution was obtained; 97.92% of enantiomeric excess (ee %) and 2.26 g/$m^2$ㆍh of flux. The operating pressure and the concentration of feed solutions were respectively 1.0 kgf/$\textrm{cm}^2$ and 0.49 mmol/L.

• Applied Biological Chemistry
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• 제41권1호
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• pp.60-66
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• 1998

10종의 식용 버섯 자실체에 대한 항보체 활성 검색에서 가장 높은 활성을 나타낸 양송이버섯 자실체를 대상으로 항보체 활성물질의 추출조건을 조사하였다. 최적조건으로서 5% urea가 함유된 0.1 N NaOH 용액으로 $65^{\circ}C$에서 2시간동안 양송이버섯 자실체를 추출한 후 methanol 가용획분이 제거된 조다당 획분 AB-0를 얻었다. AB-0는 1 mg/ml에서 90% 이상의 높은 항보체활성을 나타내었으며 마우스에 이식된 sarcoma-180에 대하여 74%의 저지효과를 보였다. AB-0를 acetone 농도에 따른 침전 분획을 실시하여 AB-20, AB-40, AB-60, AB-80, AB-A의 5개의 획분을 얻었다. 이중 가장 높은 항보체활성과 수율을 나타낸 AB-20 획분은 탄수화물 39%, 단백질 46%를 함유하였으며, 구성당은 glucose, arabinose, xylose, galactose, mannose가 6.49 : 1.98 : 1.24 : 1.00 : 0.71의 비율로 존재하였고 구성아미노산은 isoleucine(12.60%), glutamic acid+glutamine(12.45%), valine(11.79%), alanine(11.46%), leucine(10.19%)와 aspartic acid, asparagine(10.56%) 등 이었다. Pronase 처리한 AB-20에서는 78.5%의 항보체활성을 나타내고 대조군에 비해 14.5% 감소하였으나, AB-20의 periodate 산화물에서는 활성이 42.6%로 50% 이상 크게 감소하였다. 따라서 양송이 버섯의 알칼리 추출물중 가장 강한 활성을 보인 AB-20의 주요 활성부위는 다당류이며 단백질도 일부 관여하는 것으로 생각되었다.

• 한국버섯학회지
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• 제12권3호
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• pp.193-200
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• 2014

일반성분 중 조회분 함량은 버섯이 균사체 보다 높은 조회분 함량을 보였다. 조단백질은 모든 시험구에서 19% 내외로 나타났고, 조지방 함량은 동충하초 균사체에서 가장 높았으며, 조섬유는 팽이버섯에서 가장 높게 나타났다. 가용성물질소물은 팽이버섯균사체와 동충하초균사체에서 60% 이상의 함량을 나타내었다. 각 시료별 총단백질과 총당의 구성 비율은 팽이버섯과 팽이버섯 균사체의 단백다당류의 구성이 다름을 확인하였다. 시료별 핵산관련물질 함량은 팽이버섯에서 5'-GMP, 5'-XMP, 5'-IMP순으로 높게 나타났고, 팽이버섯 균사체는 5'-XMP, 5'-GMP, 5'-IMP, 동충하초 균사체는 5'-XMP, 5'-IMP, 5'-GMP순으로 높게 나타났다. 모든시료에서 각각 17종의 아미노산이 검출되었다. 각 시료의 총 아미노산 함량을 살펴보면 팽이버섯 18,980 mg%, 팽이버섯 균사체 17,604 mg%, 동충하초 균사체 18,429 mg%로 나타났고 모든 시료에서 glutamic acid 함량이 가장 높았다. 유리아미노산 함량도 구성아미노산과 유사하게 팽이버섯, 팽이버섯 균사체, 동충하초 균사체 순으로 높게 나타났다. 본 실험결과 팽이버섯, 팽이버섯 균사체 및 동충하초 균사체는 핵산관련 물질과 아미노산 함량이 높게 나타나, 아미노산 함유 식품 개발에 적합한 자원으로 활용도가 기대된다.

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 2003년도 봄 학술발표회 논문집
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• pp.179-182
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• 2003

Chitin is the most abundant natural amino polysaccharide and is estimated to be produced annually almost as much as cellulose. It has become of great interest not only as an under utilized resource, but also as a new functional material of high potential in various fields. In addition, chitin and chitosan are recommended as suitable functional materials, because these natural polymers have excellent properties such as biocompatibility, biodegradability, non-toxicity and adsorption properties, etc. (omitted)

• Journal of Life Science
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• 제9권1호
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• pp.69-80
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• 1999

Some trials to alter the structure of extracellular polysaccharides by means of biotransformation and microbial modification have been reported. Seaweed alginate was acetylated by intact and resting cells of Pseudomonas syringae ATCC 19304. Glucose analogs such as 3-O-methyl-D-glucose used as sole carbon sources was directly incorporated into curdlan by agrobacterium sp. ATCC 31749. The 2-amino-2-deoxy-D-glucose (glucosamine)and 2-acetamido-2-deoxy-D-glucose (N-acetylglucosamine) were incorporated into microbial cellulose by Acetobacter xylinum ATCC 10245. The changed monomeric composition in pullulan by Aureobasidium pullulans ATCC 42023 as well as zooglan by Zoogoea ramigera ATCC 25935 was another effect of glucose analogs used a carbon source. There was no effect of glucose analogs found in polysacharide-7 (PS-7) produced by Beijerinckia indica. ATCC 21423.

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 1998년도 봄 학술발표회 논문집
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• pp.329-333
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• 1998

The major classes of antimicrobial agents for textiles include organo-metallics, phenols, quaternary ammonium salts, and organo-silicones. These finishes should be durable, have selective activity towards undesirable organisms, be compatible with other finishes and dyes, and be nontoxic to man [1]. Chitosan, as a deacetylated derivative of chitin, is a natural, non-toxic and biodegradable polymer. Chitosan is also known as an antimicrobial polysaccharide due to antimicrobial action of the amino group at the C-2 position of the glucosamine residue.(omitted)

• 한국해양바이오학회지
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• 제4권1호
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• pp.15-23
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• 2010

Seaweed has been consumed especially in the Asia since centuries ago as a vegetable (Matsukawa, 1997; Burtin, 2000; Raghavendran 2003). Seaweed has many valuable nutrients i.e. as a mineral resources, vitamin, and non-caloric fiber, also has potential antioxidant resources. Such of nutrition consists in the Seaweed is Chlorophyll, Carotene, Fucoxantine, Luteen and Zeaxantine, Polysaccharide and Diet Fiber, Mineral, Protein and Amino Acid, Fat and Fatty Acid, Vitamin, Polifenol, also Methanol. The benefits of some nutrition content on seaweed are as an antioxidant, slimmer agent, anti-diabetes, healthy for heart, eyes health, Iodium source, and some kind of vitamins functions. As a conclusion, developing research about seaweed we believe it would be a part of medicine material and food processing, also cosmetic industrial.

• 한국균학회지
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• 제8권2호
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• pp.107-113
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• 1980

To investigate antitumor components of Korean higher fungi, the carpophores of Ganoderma lucidum (Fr.)Karst. were collected at Gal-mae-ri in Gyeong-gi Province and extracted with 0.1N sodium hydroxide solution. The extract was purified by dialyzing through Visking tube and a dark brownish power was obtained. The antitumor activity of the fraction was tested against sarcoma 180 implanted in mice. The tumor inhibition ratio of the fraction against the tumor was 87.6% in doses of 50 mg/kg/day for the period of ten days. The tumor in five of 15 mice was completely regressed. The antitumor fraction was chemically analyzed and found to be a complex of polysaccharide and protein, from which four monosaccharides and eighteen amino acids were identified.

• Mycobiology
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• 제34권4호
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• pp.230-235
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• 2006

Fruiting bodies of Phellinus linteus were extracted by hot water and alkali methods. Sugar contents of PL-H (hot water extract) and PL-A (alkali water extract) were 81.1%, 37.4% and protein contents were 6.2%, 21.8%, respectively. Amino acid pattern showed that two extracts contained large amount of aspartic acid and alanine. Two extracts showed characteristic IR absorption pattern for glycosidic bond at $890\;cm^{-1}$. PL-H was divided two fractions by gel filtration chromatography and the molecular weights of each fraction were estimated to be about 10 kD and 225 kD, respectively and also PL-A was estimated 10 kD. Two extracts showed strong antitumor, immunomodulating and antioxidant activities, and were compared with commercialized glycopeptide anticancer drugs.

• 한국막학회:학술대회논문집
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• 한국막학회 1995년도 춘계 총회 및 학술발표회
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• pp.46-47
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• 1995

Chitosan, an unbranched (1-4)-linked-2-amino-2-deoxy-$\beta$-D-glucan, is prepared by chemical N-deacetylation of chitin, which is the main structure element of the cuticles of crab, shrimp, and insects, and is found in the cell walls of bacteria. It has the same main chain in its molecule like that of natural heparin. Heparin, an anionic polysaccharide, is the best of anticoagulants that have been found so far. In this study, to improve the blood compatibility of chitosan, 2-(methacrylovloxy)-ethyl-2-(trimethylammonium)ethyl phosphate(MTP) with phospholipid polar groups was grafted on the chitosan membranes and the biochemical properties the MTP-grafted chitosan membranes were investigated.