• Journal of Microbiology
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• 제45권1호
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• pp.58-63
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• 2007

In this study, the relative synonymous codon and amino acid usage biases of the broad-host range phage, KVP40, were investigated in an attempt to understand the structure and function of its proteins/protein-coding genes, as well as the role of its tRNAs. Synonymous codons in KVP40 were determined to be AT-rich at the third codon positions, and their variations are dictated principally by both mutational bias and translational selection. Further analysis revealed that the RSCU of KVP40 is distinct from that of its Vibrio hosts, V. cholerae and V. parahaemolyticus. Interestingly, the expression of the putative highly expressed genes of KVP40 appear to be preferentially influenced by the abundant host tRNA species, whereas the tRNAs expressed by KVP40 may be required for the efficient synthesis of all its proteins in a diverse array of hosts. The data generated in this study also revealed that KVP40 proteins are rich in low molecular weight amino acid residues, and that these variations are influenced primarily by hydropathy, mean molecular weight, aromaticity, and cysteine content.

• 대한화학회지
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• 제7권1호
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• pp.6-12
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• 1963

Amino acid composition of Makjang was determined by combined usage of ion exchanged resin and paper chormatography in the following states. a. A fraction soluble in water b. Hydrolysate of the whole Makjang c. Same as a. (p.p.t. formed by tungstic acid or trichloroacetic acid being removed) d. Hydrolysate of c. (T and TCA) By comparing amino acid composition of Makjang with that of its raw material, we found that decomposition of essential amino acids during brewing is slight. From the amino acid composition of a,b,c,d, we discussed the ratio of amino acid liberation during brewing and assumed that Makjang contains peptide-like substances composing of glutamic acid and aspartic acid.

• Applied Biological Chemistry
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• 제36권6호
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• pp.525-532
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• 1993

쌀의 주요 저장 단백질중의 하나인 알콜용해성 프롤라민의 성질을 분석하고 이들을 동정하기 위하여 유전자 database로부터 얻은 17개의 프롤라민 유전자의 염기서열을 상호비교 분석하였다. 유전자로부터 유추된 단백질 서열의 다중분석결과 프롤라민들은 계통발생적으로 type에서 type IV의 4개의 군으로 크게 분류할 수 있었다. 이러한 분류는 아미노산 서열 중간과 카복실 말단쪽의 짧은 결손에 의해 여러 형태의 아미노산 서열에 의한 것임을 알 수 있었다. 1군에서 4군까지의 군들은 meththionine과 cysteine과 같은 황을 포함하는 아미노산이 각각 1, 4, 10, 30%로 구성된 특징을 보여 주었다. 또한 각 군들은 등전점이 9.2, 8.2, 6.7, 7.4인 각군별로 매우 상이한 등전점을 나타내었다. 아울러 GC3s에 대한 유효 암호수(effective codon number, Nc)와 우선 암호수(preferred codon number)의 분석과 상관 그래프를 통해서 프롤라민 유전자들의 군별 전이 효율의 차이가 현저하여 프롤라민 생산 수준의 군별차이의 가능성을 제시하였다.

• BMB Reports
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• 제37권4호
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• pp.487-492
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• 2004

The extent of codon usage in the protein coding genes of the mycobacteriophage, Bxz1, and its plating bacteria, M. smegmatis, were determined, and it was observed that the codons ending with either G and / or C were predominant in both the organisms. Multivariate statistical analysis showed that in both organisms, the genes were separated along the first major explanatory axis according to their expression levels and their genomic GC content at the synonymous third positions of the codons. The second major explanatory axis differentiates the genes according to their genome type. A comparison of the relative synonymous codon usage between 20 highly- and 20 lowly expressed genes from Bxz1 identified 21 codons, which are statistically over represented in the former group of genes. Further analysis found that the Bxz1- specific tRNA species could recognize 13 out of the 21 over represented synonymous codons, which incorporated 13 amino acid residues preferentially into the highly expressed proteins of Bxz1. In contrast, seven amino acid residues were preferentially incorporated into the lowly expressed proteins by 10 other tRNA species of Bxz1. This analysis predicts for the first time that the Bxz1-specific tRNA species modulates the optimal expression of its proteins during development.

• Genomics & Informatics
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• 제14권4호
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• pp.234-240
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• 2016

Different mechanisms, including transcriptional and post transcriptional processes, regulate tissue specific expression of genes. In this study, we report differences in gene/protein compositional features between apoptosis involved genes selectively expressed in human tissues. We found some correlations between codon/amino acid usage and tissue specific expression level of genes. The findings can be significant for understanding the translational selection on these features. The selection may play an important role in the differentiation of human tissues and can be considered for future studies in diagnosis of some diseases such as cancer.

• 대한지역사회영양학회지
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• 제13권1호
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• pp.134-142
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• 2008

The objective of the present study was to determine the prevalence and type of nutritional ergogenic aids use, and to determine the frequency, reasons for use of nutritional ergogenic aids. Thirty-four male bodybuilders (mean age = 27.0 years), twenty-four male weight lifters (mean age = 20.9 years) participated in the study. Participants completed a comprehensive survey detailing their usage patterns. In this study, 78.1% of bodybuilders and 79.2% of weight lifters reported using nutritional ergogenic aids. The most frequently taken nutritional ergogenic aids, in ranking order, were protein/amino acid powders (79.4%), multivitamin/minerals (67.7%) and creatine (67.6%) for bodybuilders, in contrast to sports drinks (100.0%), protein/amino acid powders (50.5%) and creatine (50.5%) for weight lifters. Over the half of the respondents, 79.4% of bodybuilders and 50.6% of weight lifters, used protein/amino acid powders to gain muscle mass and to stay healthy. Bodybuilders, 67.6% and weight lifters, 41.7%, used multivitamin/minerals to stay healthy and for energy. The intakes of most vitamin and minerals through diet and nutritional ergogenic aids were much greater than RDA. Vitamin $B_1$, vitamin $B_2$, niacin, vitamin $B_6$ and folate intakes were ranged at 400-900%. Vitamin C intake was 1285.4% (for bodybuilders) and 1322.6% (for weight lifters). The correct answer rate of nutritional ergogenic aids was 46.0% for bodybuilders and 52.0% for weight lifters. Both bodybuilders and weight lifters took highly nutritional ergogenic aids and it tended to be taken irrespective of scientific background. Specific sport nutrition education applicable to athletes, especially strength athletes, is recommended. The findings of this investigation could be used to enable the professionals (sports dietician and physician) to identity common misconceptions regarding nutritional ergogenic aids and to implement educational programs.

• Parasites, Hosts and Diseases
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• 제42권4호
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• pp.159-167
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• 2004

Sequences of the complete protein-coding portions of the mitochondrial (mt) genome were analysed for 6 species of cestodes (including hydatid tapeworms and the pork tapeworm) and 5 species of trematodes (blood flukes and liver- and lung-flukes). A near-complete sequence was also available for an additional trematode (the blood fluke Schistosoma malayensis). All of these parasites belong to a large flatworm taxon named the Neodermata. Considerable variation was found in the base composition of the protein-coding genes among these neodermatans. This variation was reflected in statistically-significant differences in numbers of each inferred amino acid between many pairs of species. Both convergence and divergence in nucleotide, and hence amino acid, composition was noted among groups within the Neodermata. Considerable variation in skew (unequal representation of complementary bases on the same strand) was found among the species studied. A pattern is thus emerging of diversity in the mt genome in neodermatans that may cast light on evolution of mt genomes generally.

• BMB Reports
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• 제34권6호
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• pp.502-508
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• 2001

A cDNA, encoding the human growth hormone (hGH), was synthesized based on the known 191 amino acid sequence. Its codon usage was optimized for a high level expression in Escherichia coli. Unique restriction sites were incorporated throughout the gene to facilitate mutagenesis in further studies. To minimize an initiation translation problem, a 624-bp cassette that contained a ribosome binding site and a start codon were fused to the hGH-coding sequence that was flanked between the EcoRI and HindIII sites. The whole fragment was synthesized by an overlapped extension of eight long synthetic oligonucleotides. The four-short duplexes of DNA, which were first formed by annealing and filling-in with a Klenow fragment, were assembled to form a complete hGH gene. The hGH was cloned and expressed successfully using a pET17b plasmid that contained the T7 promoter. Recombinant hGH yielded as much as 20% of the total cellular proteins. However, the majority of the protein was in the form of insoluble inclusion bodies. N-terminal amino acid sequencing also showed that the hGH produced in E. coli contained formyl-methionine. This study provides a useful model for synthesis of the gene of interest and production of recombinant proteins in E. coli.

• International Journal of Industrial Entomology and Biomaterials
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• 제25권1호
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• pp.93-98
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• 2012

Nutrition value of mealworm, Tenebrio molitor was analyzed due to increasing demand of usage as a protein source for domestic animals and even further for human consumption. The purpose of the present work was to determine the chemical compostion of the Tenebrio molitor larvae, adult that were maintained under standard condition for further usage of mass-rearing system and its exuvium, and excreta. Tenebrio molitor, larvae, adult, exuvium and excreta contained 46.44, 63.34, 32.87, and 18.51% protein respectively, suggested that even excreta could be used as an additional supplement in food recycling process. This protein was also rich in amino acids such as Isoleucine, leucine and Lysine which all met the nutritional value recommended by the Food and Agriculture Organization. Fatty acid composition was detected with high component of oleic acid (C18:1), along with linoleic acid (C18:2) and palmitic acid (C16) in all adult, larvae, exuvium and excreta. These oleic acid (C18:1), linoleic acid (C18:2) and palmitic acid (C16) components were the same or even highly contained in excreta of mealworm 22.29, 47.19 and 19.17% respectively. Longer chains of unsaturated fatty acids consisted of two to three double bonds are known as healthy product was recognized in large amount. These results show new ways to consume mealworms and its waste for animal and human consumption.

• 원예과학기술지
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• 제29권2호
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• pp.144-150
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• 2011

우리나라에서 널리 보급되고 있는 일반감자 '수미'와 일반감자의 단점을 보완하여 개발된 유색감자 6종의 영양성분 및 이화학적 특성을 분석하기 위해 일반성분 및 환원당, 유리당, 유리아미노산, 유기산, 무기질 그리고 색도를 측정하였다. '수미'는 유색감자에 비해 수분 함량이 높았으며, 탄수화물은 '레드'가 18.0%로 감자 중 가장 많았고, 조회분 함량은 모든 유색감자가 '수미'에 비해 높게 나타났다. 또한 조단백, 조지방 함량은 '자서'에서 가장 높게 나타났으며, 조섬유 함량은 '블루'가 1.6%로 가장 높았고, 그 함량은 '수미'의 2배였다. 환원당 함량은 유색감자 중 '로즈'와 '블루'가 '수미'의 3배 이상이었으며, fructose와 glucose가 대부분인 '수미'에 비해 유색감자는 sucrose의 함량이 높았다. 유리 아미노산은 glutamic acid와 arginine, ${\gamma}$-aminobutyric acid가 대부분을 차지하였으며, 유리당은 품종 간 그 함량과 조성이 매우 다양했다. 또한 유기산 함량은 '하령'이 $507.1mg{\cdot}100g^{-1}$으로 가장 높았으며, 무기질은 모든 감자에서 칼륨이 대부분을 차지했다. 색도를 측정한 결과, 명도를 나타내는 Hunter 'L' value는 '자서'에서, 적색도를 나타내는 Hunter 'a' value는 '블루'와 '자심'에서 높았으며, 황색도를 나타내는 Hunter 'b' value는 단면이 노란색을 띄는 '하령'에서 가장 높았다. 이상의 결과로 유색감자는 '수미'에 비해 많은 양의 영양성분을 함유하고 있고 우수한 품질 특성을 나타내어 기능성 식품소재로써의 이용성이 높을 것으로 기대된다.