• Journal of Families and Better Life
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• v.21 no.1
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• pp.113-127
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• 2003

This paper aims to provide the basic data about the uses of agricultural products related to food, cloth and shelter in the late Joseon Dynasty by analysing$\ulcorner$Kyuhap-Chongseo$\lrcorner$(1809). The results of this study are as follows: 1. The agricultural products related to cloth, food and shelter were of 95 species total(horticultural crops 37.9%, industrial crops 22.1%, food crops 13.7%, floricultural crops 13.7%. and animal products 12.6%). 2. The agricultural products(24species) related to clothing were used for weaving, dyeing, laundering and starching textiles. 80 species of agricultural products were used as ingredients in about 140 dishes. As for shelter, the agricultural products(30species) were used for making stationaries. managing vessels and illumination, and building structures.

• Korean Journal of Veterinary Research
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• v.36 no.1
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• pp.119-129
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• 1996

The present study was conducted to rapidly detect Listeria monocytogenes in raw milk. Specificity and sensitivity of polymerase chain reaction(PCR) technique, and direct PCR were examinded in raw milk, also were compared the calssical culture methods with PCR technique. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L nomocytogenes. In the PCR specificity tests, each of the 10 strains of L monocytogenes tested gave a single 70-bp band. But the other six Listera spp tested gave negative results. Results of the sensitivity tests showed that as few as 2 CFU of L monocytogenes in pure cultures could be detected with 16S rRNA-based primers, L-1 and L-2. In different PCR cycles, a PCR product was detected with $10^3$ cells of L monocytogenes from 25 cycles to 50 cycles and the concentration of PCR products was cycle-dependent. Raw milk samopes added L monocytogenes cells gave negative results. However, these samplers gave a single 70-bp band by pretreatment of pronase, and PCR products were detected with $10^1$ cells of L monocytogenes. To detemine the most sensitive culture protocol to use in conjunction with the PCR assay, raw milk samples were inoculated with L monocytogenes at concentrations ranging from 1 to $5.7{\times}10^4CFU/ml$. PCR assays from Listeria enrichment broth(LEB) containing raw milk samples added L monocytogene EGD could dtect 10 cells in pronase-pretreated samples without incubation, and 1 cell of L monocytogenes in both 12 hr and 24 hr incubation, respectively. Isolation raw of PCR assays was similar to that of classical culture methods, but required time for detection of L monocytogenes could remarkably be reduced compare to culture methods.