• Environmental Engineering Research
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• 제24권3호
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• pp.397-403
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• 2019

Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.

• 대한금속재료학회지
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• 제47권3호
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• pp.188-194
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• 2009

Recently, new extraction technology for manganese nodule has been developed as alternative noble metallic resources. It is important to understand thermodynamic behaviors of phosphorus in low basic slag system from the viewpoint of the refining processing optimization. Thermodynamic behaviors of phosphorus in the $FeO-MnO-CaO-SiO_2-MgO_{satd.}$ slag system were investigated at 1723 K with various oxygen potential and slag composition of low basicity. The experimental results for dependence of phosphorus on oxygen potential and slag basicity indicated that the dissolution mechanism of phosphorus into slag of low basicity could be derived as follows; $[P]+5/4O_2+(O^{2-})=({PO_{3.5}}^{2-})$ Present experimental results implied that stability of phosphorus in slag would be depended on both of $O^{2-}$ (basicity) and content of $Ca^{2+}$ in molten slag. The thermodynamic effect of FeO, MnO and $Na_2O$ on low basicity on phosphate capacity was discussed.

• 대한금속재료학회지
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• 제47권5호
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• pp.304-310
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• 2009

Recently, manganese nodule has been focused on alternative resources because of its high grade of noble metallic elements such as Co, Ni, and Cu etc. From the viewpoint of an optimization the operating variables for energy efficiency of smelting reduction process, thermodynamic model for smelting reduction process of Manganese nodule was developed by using energy and material balance concept. This model provided that specific consumption of pure oxygen and coke was strongly depended on post combustion ratio (PCR) and heat transfer efficiency (HTE). The dressing and dehydrating process of low grade manganese can be proposed an essential process to minimize the specific energy consumption with decreasing slag volume. The effect of electricity coal base smelting reduction process was also discussed from the energy optimizing point of view.

• Fisheries and Aquatic Sciences
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• 제23권3호
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• pp.9.1-9.6
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• 2020

Background: Fucosterol is a compound commonly found in algae that has various biological activities. The purpose of this study was to develop a high-performance liquid chromatography (HPLC) validation method for fucosterol and to compare the fucosterol contents of 11 algal species from Ulleungdo, Korea. Method: In this study, we successfully isolated and identified fucosterol from a 70% EtOH extract of Sargassum miyabei, and subsequently conducted specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision analyses for development of an HPLC validation method. Fucosterol contents were compared using the established HPLC validation conditions. Results: We successfully isolated fucosterol from a 70% EtOH extract of S. miyabei and identified it based on spectroscopic analysis. On the basis of HPLC validation using the fucosterol isolated from S. miyabei, we confirmed specificity (8.5 min), linearity (R² = 0.9998), LOD (3.20 ㎍ mL^-1), LOQ (9.77 ㎍ mL^-1), accuracy (intra-day and inter-day variation, 90-110%), and precision (RSD, 1.07%). Fucosterol contents in the 11 assessed algal species ranged from 0.22 to 81.67 mg g^-1, with the highest content being recorded in a 70% EtOH extract of Desmarestia tabacoides (81.67 mg g^-1), followed by that of Agarum clathratum (78.70 mg g^-1). Conclusions: The results indicate that 70% EtOH extracts of D. tabacoides and A. clathratum containing fucosterol with various effects can be potential alternative sources of fucosterol.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.32-32
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• 2019

The aim of this study was to provide basic guidelines for conservation and management of endangered plants in the national parks of Korea. Iris dichotoma Pall. (Iridaceae), which is a popular garden plant, is considered a second-class endangered species by Korean government and it is listed as a EN (Endangered) species in Red Data Book of Korea. We analyzed ecological conditions of I. dichotoma habitats based on vegetation properties and soil characteristics. This species which is known to inhabit in grassland adjacent to the ocean of lowlands slope and its population was located at an elevation of 8 m to 11 m. In the study sites, the mean of soil organic matter, total nitrogen and soil pH were 6.16%, 0.234% and 5.39 respectively. Additionally, the genetic variation and structure of three populations were assessed using ISSR (Inter Simple Sequence Repeat) markers. The genetic diversity of I. dichotoma (P = 59.46%, H = 0.206, S = 0.310) at the species level was relatively high. Analysis of molecular variance (AMOVA) showed 82.1% of the total genetic diversity was occurred in within populations and 17.9% variation among populations. Lastly, we developed predicted distribution model based on climate and topographic factors by applying SDMs (Species Distribution Models). Consequently, current status of I. dichotoma habitats is limited with natural factors such as the increase of the coverage rate of the herbs due to ecological succession. Therefore, it is essential to establish in situ and ex situ conservation strategies for protecting natural habitats and to require exploring potential and alternative habitats for reintroduction.

• BMB Reports
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• 제36권2호
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• pp.230-236
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• 2003

Hot water-soluble crude polysaccharide (HCAP-0) that was obtained from the fruits of Capsicum annuum showed potent anti-complementary activity. The activity was unchanged by pronase digestion, but decreased by periodate oxidation. The HCAP-0 was fractionated by DEAE ion-exchange chromatography to give two major fractions, HCAP-II and III. These two fractions were finally purified by gel filtration to give HCAP-IIa, HCAP-IIIa1, and IIIa2 fractions that had high anti-complementary activities. The HCAP-IIIa1 and IIIa2 consisted of homogeneous polysaccharides. The anti-complementary activities were unaffected by treatment with polymyxin B, indicating that the modes of complement activation were not due to preexisting lipopolysaccharide. The molecular weight and sugar content of HCAP-IIIa2 had potent anti-complementary activity. The highest yields were 55 kDa and 75.9%, and the molar ratio of galactose (Ara:Gal, 1.0:4.6) was higher than other sugars. The crossed immuno-electrophoresis showed that both classical and alternative pathways were activated by HCAP-IIIa2.

• 한국조경학회지
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• 제28권4호
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• pp.117-124
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• 2000

This paper presents a design of urban waterfront park using agricultural reservoir in urban fringe area to meet increasing leisure demand for urban resident and to improve resident's quality of life through preventing the reservoir from urban use after reclamation. The site, Unam-Ji, is located on northern part of Taegu metropolitan area, having 17,791 square meters. We had designed this park from 1997 to 1998. After analyzed such factor as accessibility, current land uses, topography, hydrology, vegetation and landscape, climate, and soil, we constructed basic design scheme and principes such as conservation of natural resources, meeting user's recreation demands, providing opportunities for experiencing natural process, integrating naturalness and amenity of the site, and comfortable place with natural dynamism. Based on these principles, we have designed Unam-Ji waterfront park having intrinsic characteristics of the site, maintaining water quality and ecological restoration and improving water-based recreation opportunities. After evaluation of such criteria of design alternative as land use and circulation, spatial organization, convenience for use of and maintenance, conservation of natural environment, and degree of achieving planning and design goals, and maintaining landscape, we have visualized and formalized waterfront park in design process, which is consisted of four squares-- green square for multiple uses, water-based recreation square, pedestrian roads and decks near waterfront, performance square and fords near waterfront. In conclusion, this waterfront park design contributes to improve quality of urban development through preserving agricultural as an important component of urban water system and provide important planning and design implications in urban open space planning.

• 자원ㆍ환경경제연구
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• 제10권1호
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• pp.1-23
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• 2001

This paper reviews a single trip decision to visit individual sites of a natural park within a random utility framework as an alternative to travel cost model. The model is applied to a 1998 survey of on-site visitors to Mu Deung Mt. neighboring Kwang Ju. city. Out of four sites considered, the model seemed to provide a reasonable description of behavior for three sites. In addition to travel costs as an implicit price, variables reflecting characteristics of each site also influenced decisions to visit the site. Moreover, benefits estimates derived from the RUM implied that visitors placed three times higher values on a site based on cultural inheritance compared with those of two recreational sites.

• 유기물자원화
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• 제7권1호
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• pp.53-66
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• 1999

폐기물 발생량 증가뿐만 아니라 매립장 고갈과 재정부족으로 인해 폐기물처리비용 절감을 위한 계획적인 접근이 요구되고 있다. 본 연구에서는 지리적으로 연접한 경상남도 창원, 마산, 진해 3개 도시의 주거 및 상업 지역으로부터 발생하는 음식물쓰레기를 적정하게 관리하기 위한 대안들을 설정한 후 비용평가를 위해 WRAP모델을 적용하여 다음과 같은 결과를 얻었다. 1, 집단급식소, 음식점, 시장 등 상업시설에서의 음식물쓰레기 발생량은 비교적 적은 반면에 가정에서 발생하는 음식물쓰레기가 약 80%로 대부분을 차지하였다. 2. 음식물쓰레기 관리를 위한 톤당비용은 발생원분포 및 처리시설 위치 등의 각 도시별 상황에 따라 차이가 있었으며, 특히 해안매립지 조성에 많은 비용이 소요된 진해시에서 톤당비용이 가장 높게 나타났다. 3. 음식물쓰레기 관리대안별 비교검토에서 사료판매수입을 포함하는 경우 음식물쓰레기 사료화대안이 가장경제적인 것으로 나타났으며, 톤당비용이 매립대안의 60~70%로 나타났다. 또한 음식물쓰레기 퇴비화대안이 매립보다 경제적이었으며, 소각대안이 가장 비용이 많이 소요되는 것으로 나타났다. 4. 3개 도시지역이 공동으로 음식물쓰레기 처리시설들을 운영하는 광역적 관리시스템인 경우 사료화를 제외하고는 개별적 독립관리시스템으로 운영하는 대안들보다 경제적인 것으로 나타났다. 이에 따라 광역적인 관리시스템에 대한 검토가 요구된다.

• 대한기계학회논문집B
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• 제28권12호
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• pp.1467-1474
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• 2004

Recently, with respect to an increase of energy consumption, many countries have tried to develop alternative fuels. In the United States, less than 10 percent of motor fuels will be displaced by alternative fuels by year 2010, with about 25 percent of the replacement fuels from renewable resources. But the level of exhaust gas is not decreased to the result of an increase of diesel vehicles. Moreover, emission regulations are being intensified by advanced countries such as America and Europe. Because Biodiesel is similar to diesel fuel, it is essential to judge the environmental and health effects deriving from the use of Biodiesel in diesel engine. Therefore, this research could be conformed whether both Biodiesel 20% and Biodiesel 100% are influenced on emission according to sulfur contents by applying DOC. As a result of using the Biodiesel, this research could be conformed though Nox was increased, CO, HC and PM were decreased, and also estimated to compare diesel fuel with the Biodiesel in accordance with engine performance and emission characteristics.