• Journal of the Speleological Society of Korea
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• no.61
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• pp.17-46
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• 2000

Mt. Jabyeong(872.5m), limestone region in the Taebaek mountains, located in 37° 32'N, 129° 25'E and in Sangye-ri, Okgye-myeon, Gangreung-si and Samok-ri, Imgye-myeon, Jeongseon-gun, Gangwon-do. The purposes of this study were to investigate the floristic composition from 1997 to 1998 in Mt. Jabyeong and report the distribution of Korean endemic plants, rare and vulnerable plants, calcicoles and calci fuges, The vascular plants in this area were composed of 93 families, 313 genera, 3 subspecies, 57 varieties, 13 forms, 495 species, totaling 568 taxa. Among the vascular plants, 21 taxa were Korean endemic plants and 7 taxa were rare and vulnerable plants. Calcicole and calcifuge plants in this study area were composed of 21 families, 30 genera, 36 species and 14 families, 15 genera, 17 species. The soil pH values of study site in Mt. chabyoung were ranging 7.4∼8, which showed that this site was a mostly limestone area with alkaline soil. And soil depth was formed to a relative thin layer, mostly 2～4cm thickness. This site showed high content of moisture and organic matter which were ranging 40-45% and 15-25%, respectively.

• Mycobiology
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• v.31 no.3
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• pp.157-161
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• 2003

Various soil samples were collected from twenty-four areas of ten different poultry farms in Korea and screened for prevalence of keratinolytic fungi. Fourteen species of feather-associated fungi belonging to ten genera Acremonium, Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Monascus, Mucor, Penicillum, and Verticillium isolated from poultry soils were grown on keratin medium. Especially, Aspergillus spp. populations associated with the soil sample is $1{\times}10^5$ cfu/g. A. flavus, A. fumigatus, A. niger, A. nidulans, and A. terreus could utilize keratin of chicken feather and degrade it, producing sulphydryl-containing compounds detected as keratinase, cysteine and total proteins. Keratinolytic activities of five Aspergillus species also changed the pH of the medium more alkaline than those that were less keratinolytic.

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.53-59
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• 1995

A protease isolated and purified 51 fold from the culture filtrate of a soil bacterium, Bacillus sp. KUN-17, which was appeared to be a monomeric protein with molecular weight of 38, 000 daltons, was suggested to be involved in the serine (-alkaline) protease (E.C 3.4.21.14) since its activity was selectively inhibited by phenylmethylsulfonyl fluoride (PMSF) and required 40$\circ$C and pH 10.5 for optimal condition. The half-life of the enzyme activity was 1 hr at 55$\circ$C, and the activity was maintained even under high concentrations of SDS or urea. The enzyme was indicated to perform random proteolysis from the fact that most of the chromogenic substrates employed were hydrolyzed by the enzyme. The affinity of the enzyme for natural proteins was approximately 10-times higher than ester compounds, and both substrates showed mutual inhibitory effect competitively for the enzyme activity.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.332.2-333
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• 2002

Three alkaline proteases. designated JB-1. JB-2, and JB-3, are extracellular enzymes produced by Bacillus spp. JB411 which was isolated korean soil. They were separated by DEAE-sepharose CL-6B gel. and further purified using ammonium sulfate precipitation. ultra membrane filtration. and Ultrogel AcA gel filtration. The optimun pH values of proteases IB-1. JB-2. and JB-3. were shown to be 9.5. 9.5 and 7.5. respectively. All three proteases were stable in the pH range of 5-11. (omitted)

• Mycobiology
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• v.31 no.4
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• pp.209-213
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• 2003

Aspergillus flavus K-03 isolated from poultry forming soil in Korea was studied for its ability to produce extracellular proteases on basal medium containing 2%(w/v) chicken feathers. The fungus was observed to be a potent producer of such enzymes. Keratinolytic enzyme secretion was the best at 15 days of incubation period at pH 9 and temperature $40^{\circ}C$. No relationship existed between the enzyme yield and increase of biomass. Enzyme production was suppressed by exogenous sugars in descending order arabinose>maltose>mannose>fructose. But glucose did not influence the enzyme activity. The keratinolytic enzyme released by the fungus demonstrated the ability to decompose keratin substrates as chicken feather when exogenous glucose was present. The keratinolytic activity was inhibited by $HgCl_2$ and serine-protease inhibitors such as phenymethylsulfonyl fluoride(100%), chymostain(88%), crystalline soybean trypsin inhibtor(80%), antipain(45%) and aprotinin(40%), and was not by cystein-protease and aspartyl-protease inhibitors. The enzyme activity is only partially inhibited by metallo-protease inhibitor. Thus, the enzyme secreted by A. flavus K-03 belongs to the alkaline serine-type protease.

• Journal of Korea Soil Environment Society
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• v.4 no.1
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• pp.49-55
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• 1999

The treatment of petroleum contaminated soil requires various physico-chemical remediation technologies which are efficient in time and can reduce the possibility of secondary contamination by themselves In this study, an innovated soil washing process was proposed to treat the diesel-contaminated soil. Micro-bubbles, which were generated by hydrogen peroxide, deserted and floated the contaminants. Soils less than #60(0.25mm) were artificially contaminated by 6,500mg TPH/kg dry soil initially. The process was examined for pH, the soil to water mixing ratio, concentration of $H_2O$$_2$, and contacting times. In the case of less than #60 soil, maximum removal efficiency(60%) was obtained at pH 12. 1.0% hydrogen peroxide, and 1 : 5 soil to water mixing ratio for 1 hour.

• Journal of Forest and Environmental Science
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• v.30 no.4
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• pp.331-341
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• 2014

The present study is an attempt to give an account of the inventory of plant species, phytosociological characteristics of vegetation and pedological characteristics of Rambi Beat Forest under Senchal East Forest Zone, Darjeeling, West Bengal, India. Its plant community were analyzed quantitatively and synthetically. The results reflect dominancy of dicotyledons over monocotyledons in the four studied sites The plant community comprising of 50 plant species belonging to 40 genera of 27 families. Ramhi beat reflected higher diversity of species. Maximum IVI value was recorded by Viola surpense (47.17) in Rambhi forest beat. The Berger parker index and evenness index were found to be highest for Viola surpense, Fragaria nubicola, Pilea umbrosa in Rambhi beat. The soil characteristics of the different pedons revealed alkaline nature of soil in Rambhi beat. Higher levels of soil organic carbon content reflect higher fertility of the soil of Rambhi beat. The response towards soil available nitrogen and phosphate were different among the ten pedons of Rambhi beat. Therefore, proper management and conservative measures needs to be implemented for conservation of bioresources in Senchel wildlife Sanctuary of West Bengal, India.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.3
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• pp.129-138
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• 2008

This study was carried out to examine the growth condition of Chionanthus retusa, roadside tree in Cheongyechon (CGC) and to investigate the relationship soil characteristics and tree vitality and chlorophyll contents of it. Growth condition of tree (condition of flower, leaves and branch, % of flowering, height, diameter at breast height, width, vitality and chlorophyll contents) and physiochemical relation item (pH, organic matter, K, Mg, Na, Ca, P) were investigated. The result are as follows : 1. The growth condition of flower, leaves and branch in the left side of CGC is better than the right side since the quantity of sunshine of left side of CGC is much more than the right side. 2. The average pH was alkaline. P and organic contents were much lower than the standards. 3. Tree vitality and chlorophyll contents were bad where were high user density and high buildings, such as 1, 2 area near Jong-gak and jongro 3 ga. Among the physiochemical factors of soil which affect tree vitality, K and P were found to be the main factors. Therefore, in order to improve the growth environment of roadside tree in CGC, it is needed to do periodical soil fertilizing and improve physical characteristics of soil such as, permeability and porosity by soil conditioner.

• Journal of Korean Society of Water and Wastewater
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• v.29 no.5
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• pp.543-550
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• 2015

In this study, using soil brick with combined effective microorganisms and emergent plants was identified which it can increase the effect of conservation and improvement of water. Lab-test was consist of four kind of reactors and each of reactors were A(rawwater), B(soil brick), C(emergent plant) and D(soil brick+emergent plant). Iris pseudoacorus, Phargmites australis, Typha angustifolia and Zizania latifolia were used for emergent plant. Evaluation of application on various environment were performed on agricultural waterway and pond. The pH measurement test of soil brick was performed due to evaluate whether a strong alkaline water flows out of the soil brick. Result of lab-test, removal efficiency of D was better than removal efficiency of A presenting 20.9%, 27.9% 21.5%, 33.8% and 58.4% for $COD_{Cr}$, $BOD_5$, TN, TP and TSS respectively. Removal efficiency of soil brick on agricultural waterway was revealed to be 49.5%, 45.0%, 43.7%, 37.3% and 28.6% for $COD_{Cr}$, $BOD_5$, TN, TP and TSS respectively. And removal efficiency of soil brick on the pond was revealed to be 12.7%, 10.5%, 9.32%, 10.4% and 36.3% for $COD_{Cr}$, $BOD_5$, TN, TP and TSS respectively. Result of pH measurement test of soil brick was neutral which was about 6 to 8.

• Journal of the Korean Geotechnical Society
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• v.29 no.4
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• pp.45-56
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• 2013

In this study, a blast furnace slag with latent hydraulic property is used to cement granular soils without using Portland cement. When the blast furnace slag reacts with an alkaline activator, it can cement soils. The effect of amounts of blast furnace slag and types of alkaline activator on soil strength was investigated for resource recycling. Four different amounts of slag and six different activators (two naturals and four chemicals) were used for preparing specimens. The specimens were air-cured for 3 or 7 days and then tested for unconfined compressive strength (UCS). The UCS of cemented sand with slag increased, in the order of specimens mixed with potassium carbonate, calcium hydroxide, sodium hydroxide and potassium hydroxide. Chemical alkaline activator was better than natural alkaline activator. The maximum UCS of 3-days cured specimens was 3 MPa for 16% of slag with potassium hydroxide, which corresponded to 37% of one with 16% of high-early strength portland cement. As the amount of slag increased, the UCS and dry density of a specimen increased for all alkaline activator cases. As the curing time increased from 3 days to 7 days, the UCS increased up to 97%. C-S-H hydrates were found in the cemented specimens from XRD analyses. Cement hydrates were more generated with increasing amount of slag and they surrounded sand particles, which resulted in higher density.