• Journal of Nutrition and Health
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• 제30권7호
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• pp.813-824
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• 1997

This study was designed to investigate the effect of dietary calcium and phosphate levels on calcium and bone metabolism in rats. The rats were divided into six groups and each of the groups was fed diets with different Ca/P ratios. The experimental periods were 5 weeks . There was no significant different difference in dietary intake, body weight gain, and organ weight among the groups with different calcium and phosphate intake levels. Fecal calcium excretion was not significantly different among the groups, but urinary calcium excretion was increased by the increase in Ca/P ratio. Fecal phosphate excretion was not different but urinary phosphate excretion was increased by the increase in dietary phosphate intake. There was no significant difference in serum alkaline phophatase activity and urinary hydroxyproline levels were not significantly different among the groups. The low calcium-high phosphate(0.25Ca-1.2% P) group showed the lowest total calcium content in femur and scapula. This may be due to it having the lowest Ca/P ratio among groups. The low calcium-high phosphate(0.2%Ca-1.2%P) group showed that mandible is almost lost and osteolyzed Harversian canal was expanded in femur. Results suggest that phosphate intake affects calcium and bone metabolism more with inadequate calcium nutrition that with adequate calcium intake. Thus , for normal bone growth and metabolism , adequate calcium intake and/or high Ca/P ratio are important.

• Journal of Korean Dental Science
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• 제3권1호
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• pp.32-38
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• 2010

Objective : To evaluate the relationship between the dose of Clodronate and serum level of alkaline phosphatase (ALP), calcium (Ca), and phosphate (PO4) during orthodontic tooth movement MaterialS and MethodS: A total of 18 sex-matched Wistar rats (weight=180~230g, mean age=8 weeks) were allocated into the 2.5mM Clodronate (2.5C) group, 10mM Clodronate (10C) group, or control group (n=6 for each group). After the application of a nickel-titanium closed coil spring (force of 60g) between the upper central incisors and first molars (UFM), 2.5C, 10C, or saline was injected every third day into the subperiosteum of the alveolar bone adjacent to UFM for the experimental and control groups. The animals were sacrificed 17 days later. Trunk blood was quickly collected into a heparinized tube and centrifuged at 2,000 rpm for 20 min. The plasma was used for the biochemical assays of the serum level of ALP, Ca, and PO4. Kruskall-Wallis test and Mann-Whitney test with Bonferroni correction were performed for the statistical analyses. Results : Dose-dependent increase in the level of ALP (P<0.01) and decrease in the level of Ca (P<0.001) were observed among the control, 2.5C, and 10C groups. Although there was no significant difference in PO4 between the 2.5C and 10C groups, the 10C group showed a significantly higher level of PO4 than the control group (P<0.01). Conclusion : Since Clodronate induced significant dose-dependent change in the serum level of ALP, Ca, and PO4 during orthodontic tooth movement, orthodontists should consider these biochemical markers not only as a diagnostic tool for bone turnover rate but also as a monitoring tool for orthodontic tooth movement.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제44권4호
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• pp.174-181
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• 2018

Objectives: The number of patients with medication-related osteonecrosis of the jaw (MRONJ) is increasing, but treatment remains controversial. Published papers and systematic reviews have suggested that surgical treatment is effective in patients with MRONJ. The purpose of this study was to determine whether preoperative University of Connecticut Osteonecrosis Numerical Scale (UCONNS), other serologic biomarkers, and size of necrosis are prognostic factors for outcome of surgical treatment in MRONJ. Materials and Methods: From January 2008 to December 2016, 65 patients diagnosed with MRONJ at the Department of Oral and Maxillofacial Surgery in College of Dentistry, Dankook University who required hospitalization and surgical treatment were investigated. Patient information, systemic factors, and UCONNS were investigated. In addition, several serologic values were examined through blood tests one week before surgery. The size of osteolysis was measured by panoramic view and cone-beam computed tomography in all patients. With this information, multivariate logistic regression analysis with backward elimination was used to examine factors affecting postoperative outcome. Results: In multivariate logistic analysis, higher UCONNS, higher C-reactive protein (CRP), larger size of osteolysis, and lower serum alkaline phosphate were associated with higher incidence of incomplete recovery after operation. This shows that UCONNS, CRP, serum alkaline phosphate, and size of osteolysis were statistically significant as factors for predicting postoperative prognosis. Conclusion: This study demonstrated that CRP, UCONNS, serum alkaline phosphate, and size of osteolysis were statistically significant factors in predicting the prognosis of surgical outcome of MRONJ. Among these factors, UCONNS can predict the prognosis of MRONJ surgery as a scale that includes various influencing factors, and UCONNS should be used first as a predictor. More aggressive surgical treatment and more definite surgical margins are needed when the prognosis is poor.

• Natural Product Sciences
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• 제13권1호
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• pp.23-26
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• 2007

Hepatoprotective activity of different extracts of Citrullus colocynthis L. sch. (roots) (cucurbitaceae) was investigated in albino rats by inducing hepatotoxicity with carbon tetrachloride. The alcoholic extract of Citrullus colocynthis sch. 100 mg/kg b.w. has been shown to posses significant hepatoprotective effect by lowering the serum level of transaminases (GPT & GOT), alkaline phosphate (ALP) and bilirubin (P < 0.05 to P < 0.001).

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2017년도 추계학술발표회
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• pp.76-76
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• 2017

• 약학회지
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• 제16권1호
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• pp.47-54
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• 1972

O,O-Dimethyl-S-(phthalimido)methylphosphorodithioate (PMP) is changed into phosphine, phosphate, and phosphorothioate, when it is treated with metallic zinc in acidic medium after alkaline hydrolysis. The amount of phosphine evolved is about five times as much in sulfuric acid medium as it is in hydrochloric acid. When one micro mole of hydrolyzed PMP is treated with 8 grams of metallic zinc and 30 ml of 10 N-sulfuric acid, it is changed into 0.42 micro moles of phosphine, 0.14 micro moles of phosphorothioate, and 0.44 micro moles of phosphate.

• The Korean Journal of Physiology
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• 제7권1호
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• pp.1-11
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• 1973

인삼이 생쥐의 장기 alkaline phosphatase 활성에 미치는 영향을 아기 위하여 인삼의 알코올 추출물을 Tyrode solution에 희석하여 체중 10g당 0.1mg 또는 0.2mg 씩 매일 주사하여 7일간과 14일간 처리하였다. 공장, 신장, 간, 혈청등에서의 alkaline phosphatase 활성을 sodium ${\beta}-glycerophophosphate$를 기질로 하여 각 장기의 homogenate 또는 혈청을 반응시켜 효소의 unit를 결정하였으며, 혈중 무기 인의 함량도 측정하여 다음의 결과를 얻었다. 1 숫생쥐의 7일간 처리에서 인삼추출물(0.1mg/10g) 처리군이 Tyrode solution 처리군보다 공장, 신장, 간, 혈청에서 각각 15.28%, 12.86%, 19.05%, 11.70%의 alkaline phosphatase 활성증가를 나타내었다. 2. 암생쥐의 7일간 처리에서 인삼추출물(0.2mg/10g)은 공장, 신장, 간, 혈청에서 각각 3.46%, 6.94%, 20.37%, 4.03%의 활성증가를 나타내었다. 3. 숫생쥐의 14일간 처리에서 인삼추출물(0.1mg/10g)은 공장, 신장, 간, 혈청에서 각각 15.92%, 19.76%, 10.16%, -1.63%의 활성증가를 나타내었다. 4. 암생쥐의 14일간 처리에서 인삼추출물(0.2mg/10g)은 공장, 신장, 간, 혈청에서 각각 18.89%, 24.55%, 16.97%, 27.59%의 활성증가를 나타내었다. 5. 암수 다 같이 7일간 처리에서 간의 alkaline phosphatase 활성증가현상은 14일 처리에서 약간 감소하는 반면 공장, 신장, 혈청의 효소활성증가 현상은 7일간 처리에서보다 14일간 처리에서 훨씬 촉진되었다. 6. 인삼추출물의 7일간, 14일간 처리는 수컷의 혈중 무기 인량을 각각 22.20%, 20.96% 증가시켰으며, 암컷에서는 각각 22.38%, 17.57% 증가시켰다.

• 미생물학회지
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• 제28권2호
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• pp.162-168
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• 1990

토양에서 분리한 방사균 주에서 호염기성 단백질 분해효소의 생합성과 세포분화와의 관계를 규명하고자 기균사와 포자의 형성, 그리고 단백질 분해효소의 생산에 대한 배양조건의 영향을 조사하였다. 그 결과, 기질의 농도가 단백질 분해효소, 포자, 그리고 기균사의 형성의 조절에 매우 중요하며 이것은 배양액의 pH가 산성으로 변화기 문임을 알았다. 인산염완충용액을 이용하여 배양액의 pH를 6으로부터 9로 조정하여 주었을 때 단백질 분해효소의 생성은 pH가 증가함에 따라 증가하였다. 이와 같은 결과로부터 배양액의 pH가 호염기성 단백질 분해효소 생합성의 조절에 중요한 요소로 작용한다고 판단하였다. 판단하였다.

• Journal of Yeungnam Medical Science
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• 제27권2호
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• pp.139-145
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• 2010

Cystic parathyroid adenoma is one of rare causes of hyperparathyroidism, and tends to cause increased serum level of parathyroid hormone, alkaline phosphate and serum calcium level similar to when compared to those of solid adenoma. The possibility of a coincidental appearance of primary hyperparathyrodism and nonmedullary thyroid cancer is rare, and often neglected. A 40-year-old female presented with constipation and weight loss for 3 months. The serum calcium, phosphate and alkaline phosphate were 16.6 mg/dl, 2.2 mg/dl and 505 IU/L respectively and serum parathyroid hormone level was 1556.2 pg/mL. Neck US showed mixed nodules at both thyroid lobes, and PET-CT showed a right thyroid nodule without FDG uptake. The patient was diagnosed of primary hyperparathyroidism and underwent parathyroidectomy and a total thyroidectomy. Histopathologic results revealed parathyroid adenoma with cystic change (2 cm greater diameter) in the right lower parathyroid gland and coexistent papillary carcinoma of the right lobe of thyroid gland (infiltrating, 0.3 cm in greater diameter). She remained well and serum calcium, phosphate, parathyroid hormone level were normalized within 24 hours.

• BMB Reports
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• 제30권4호
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• pp.262-268
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• 1997

The thermophilic and thermostable alkaline phosphatase was purified to near homogeneity from the osmotic lysis of Thermus caldophilus GK24, The purified enzyme had an apparent molecular mass of 108, 000 Da and consisted of two subunits of 54,000 Da. lsoelectric-focusing analysis of the purified enzyme showed a pi of 7.3. The enzyme contained two Cys residues, and its amino acids composition was quite different from that of Thermus aquaticus YT-1 alkaline phosphatase and Escherichia coli alkaline phosphatase, The optimum pH and temperature of the enzyme were 11.0-11.5 and $80^{\circ}C$ respectively. The enzyme was stable in the pH range of 9.0-12.0 at $25^{\circ}C$ for 36 h. and the half-life at $80^{\circ}C$ (pH 11.0) was 6 h. The enzyme was activated by $MgCl_2$ and inhibited by EDTA. With ${\rho}-nitrophenyl\;phosphate\;({\rho}NPP)$ as the substrate, the enzyme had a Michaelis constant $(K_m) $of $3.6{\times}10^{-5}M$, The enzyme preferentially hydrolyzed the phosphomonoester bond of AMP in ribonucleotides and glycerophosphate.