• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.11 no.3
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• pp.117-123
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• 2006

The water quality before and after a typhoon was investigated as a part of a study on the increase of organic matter and on the fundamental measures to counter chemical oxygen demand (COD) in the eutrophic Soho coastal seawaters of Gamak Bay. The dissolved oxygen (DO) saturations were <54% when water column was stratified. The DO saturation was similar at surface and in the bottom layer after a typhoon $(78\sim88%)$, and a very high DO saturation (234%) was observed in the surface water after mass phytoplankton growth. The highest values of $NH_4-N,\;NO_3-N,\;PO_4-P$, and $SiO_2-Si$ were 18.22, 38.90, 1.58, and $52.10{\mu}M$, respectively, when freshwater entered after heavy rainfall events. In addition, high concentrations of $NH_4-N,\;PO_4-P$, and $SiO_2-Si$ were detected with low DO saturations in bottom water (>5m). A maximum chlorophyll (Chl.) concentration of $311.0{\mu}gl^{-1}$ was observed after typhoon, when a high-density Scrippsiella trochoidea red tide occurred with cell density of 42,000 cells $ml^{-1}$. The algal growth potential (AGP) was high after the typhoon. Nitrogen was always a limiting nutrient for phytoplankton growth. The highest COD level was $10.55mgl^{-1}$, and the main reason of the variation in COD was likely to be phytoplankton growth $(r^2=0.612,\;p=0.000)$. Organic matter, which entered the water column when the typhoon stirred the sediments, seems to have little effects on COD increase.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.614-620
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• 2019

Microalgae have been explored as potential host species for biofuel production. Environmental factors affect algal growth and cellular composition. The effects of several key environmental factors, such as temperature, light, and pH of the medium on the growth and biochemical composition of Scenedesmus obliquus were investigated in this study. The highest growth rate of microalgae was observed at an optimal temperature of 25℃, 150 μmol/(m²·s) light intensity, and pH 10.0. The biochemical composition analysis revealed that the carbohydrate content decreased at lower (20℃) or higher temperature (35℃), whereas the protein and lipid contents increase at these temperatures. The fluctuation of light intensity significantly affected the contents of protein, carbohydrate, and lipid. The protein levels varied greatly when the pH of the medium was below 7.0. The carbohydrate and lipid contents significantly increased at pH above 7.0.

• The Korean Journal of Malacology
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• v.25 no.1
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• pp.35-40
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• 2009

The trumpet shell Charonia sauliae is an endangered and valuable species with potential for aquaculture. For artificial propagation of C. sauliae, the effects of three different food microalgae on the development, growth, and survival rate of the larvae and spat were investigated. For the larval feeding experiments, we utilized six microalgae species as food sources, namely Pavlova lutheri, Tetraselmis suecica, Nannochloris oculata, Isochrysis galbana, Chaetoceros calcitrans, and Phaeodactylum tricornutum; for the larval and spat growth and survival experiments, we utilized T. suecica, C. calcitrans, and P. tricornutum. The results showed that the temporal digestion index (TDI) for the veliger larvae was significantly different for C. sauliae fed the different microalgae species (p < 0.05), that the T. suecica, C. calcitrans, and P. tricornutum cultivars were better suited for larval consumption (p < 0.05), and that the growth and survival of the larvae and spat were significantly influenced by food type, specifically P. tricornutum (p < 0.05). Further research is needed to evaluate the effects of other microalgae species, different algal concentrations, and biochemical composition on the growth and survival of C. sauliae.

• Environmental Analysis Health and Toxicology
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• v.28
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• pp.2.1-2.7
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• 2013

Objectives Tricalcium phosphate and calcium hydrogenorthophosphate are high production volume chemicals, mainly used as foodstuff additives, pharmaceuticals, lubricants, synthetic resin, and disinfectants. Phosphate has the potential to cause increased algal growth leading to eutrophication in the aquatic environment. However, there is no adequate information available on risk assessment or acute and chronic toxicity. The aim of this research is to evaluate the toxic potential of phosphate compounds in the aquatic environment. Methods An aquatic toxicity test of phosphate was conducted, and its physico-chemical properties were obtained from a database recommended in the Organization for Economic Cooperation and Development (OECD) guidance manual. An ecotoxicity test using fish, Daphnia, and algae was conducted by the good laboratory practice facility according to the OECD TG guidelines for testing of chemicals, to secure reliable data. Results The results of the ecotoxicity tests of tricalcium phosphate and calcium hydrogenorthophosphate are as follows: In an acute toxicity test with Oryzias latipes, 96 hr 50% lethal concentration ($LC_{50}$) was >100 (measured:>2.14) mg/L and >100 (measured: >13.5) mg/L, respectively. In the Daphnia test, 48 hr 50% effective concentration ($EC_{50}$) was >100 (measured: >5.35) mg/L and >100 (measured: >2.9) mg/L, respectively. In a growth inhibition test with Pseudokirchneriella subcapitata, 72 hr $EC_{50}$ was >100 (measured: >1.56) mg/L and >100 (measured: >4.4) mg/L, respectively. Conclusions Based on the results of the ecotoxicity test of phosphate using fish, Daphnia, and algae, $L(E)C_{50}$ was above 100 mg/L (nominal), indicating no toxicity. In general, the total phosphorus concentration including phosphate in rivers and lakes reaches levels of several ppm, suggesting that phosphate has no toxic effects. However, excessive inflow of phosphate into aquatic ecosystems has the potential to cause eutrophication due to algal growth.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.910-917
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• 2015

A total of 646 strains, including green algae and diatoms, were isolated from 220 samples to screen microalgae with high lipid productivity (LP). The samples were obtained from nine habitats in Northern Xinjiang, China in June 2013. This study initially identified eight lipidrich strains, namely, Desmodesmus intermedius XJ-498, D. intermedius XJ-145, D. intermedius XJ-99, Monoraphidium pusillum XJ-489, M. dybowskii XJ-435, M. dybowskii XJ-151, Mychonastes homosphaera XJ-488, and Podohedriella falcata XJ-176, based on 18S rDNA sequencing. The strains were cultured in a photobioreactor for the same period. Results showed that the specific growth rate (day^-1) of M. pusillum XJ-489 was the highest (1.14 ± 0.06), and the biomass concentration (g/l) of D. intermedius XJ-99 was the highest (2.84 ± 0.3). Futhermore, the lipid content (%) of M. dybowskii XJ-151 was the highest (33.5 ± 4.38), and the lipid productivity (mg l^-1 day^-1) of My. homosphaera XJ-488 was the highest (86.41 ± 9.04). C16 to C18 accounted for 86% to 98% of the total lipid, and the biodiesel qualities of the selected algae corresponded to international standards. This study suggests that My. homosphaera XJ-488, D. intermedius XJ-99, and M. dybowskii XJ-151 are the most potential strains for biodiesel production among all the isolated strains.

• ALGAE
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• v.25 no.4
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• pp.205-216
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• 2010

The marine red alga Kappaphycus alvarezii is a major source of $\kappa$-carrageenan. It has been introduced in 20 countries including India. Recently, several reports have expressed concerns about Kappaphycus invasion on Acropora corals at Kurusadai Island in the Gulf of Mannar, India, which is part of a marine bioreserve. To understand the extent of the Kappaphycus invasion, 27 randomly selected locations around Kurusadai Island and the mainland coast were surveyed during May-August 2008 and July 2009. Our rigorous sampling revealed that K. alvarezii was confined to two different patches of 105 m $\times$ 55 m and 8 m $\times$ 9 m located at the southeastern part of Kurusadai Island. The actual extent of the K. alvarezii canopy coverage was 76.7 $m^2$, accounting for less than 0.0035% of the total coral reef area. The daily growth rate of the K. alvarezii at Kurusadai was 0.7%. K. alvarezii was not observed in the coral reef area of the adjoining Pullivasal and Poomarichan Islands or the Palk Bay area cultivation sites. The lack of functional reproductive cycle, low spore viability, and the absence of microscopic phases in the life cycle of this alga coupled with the abundance presence of herbivores may restrict the further spread of this alga, so its invasive potential at Kurusadai Island is considered remote.

• Ocean and Polar Research
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• v.33 no.2
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• pp.99-111
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• 2011

To estimate the effects of limitation nutrients for phytoplankton growth and its influences on short-term variations of a winter phytoplankton community structure, we investigated the abiotic and biotic factors of surface and bottom waters at 20 stations of inner and offshore areas from 6 to 7 February in Gwangyang Bay, Korea. Also, several algal bio-assay studies were conducted to identify any additional nutrient effects on phytoplankton assemblage using surface water for the assay. The dominant species in the bay was diatom Skeletonema costatum, which occupied more than 70% of total species in most stations (St.1-16) of the inner bay. According to a cluster and multidimensional scaling (MDS) analysis based on phytoplankton community data from each station, the bay was divided into three groups. The first group included stations from the south-western parts of Myodo lsland, which can be characterized as a semien-closed eutrophic area with high phytoplankton abundance. The second group included most stations from the north-eastern part of Myodo lsland, influenced indirectly by surface water currents from offshore of the bay. The standing phytoplankton crops were lower than those of the first group. The other cluster was restricted to samples collected from offshore of the bay. In the bay, silicon (Si) and phosphorus (P) were not a major limiting factor for phytoplankton production. However, since the DIN: DIP and DSi: DIN ratios clearly demonstrated that there were potential stoichiometric N limitations, nitrogen (N) was considered as a limiting factor. Based on the algal bio-assay, in vivo fluorescence values in N (+) added experiments were higher compared to control and P added experiments. Our results suggested that nitrogen may act as one of the most important factors in controlling primary production during winter in Gwangyang Bay.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.432-438
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• 2018

Microalgae hold promise as a renewable energy source for the production of biofuel, as they can convert light energy into chemical energy through photosynthesis. However, cost-efficient harvest of microalgae remains a major challenge to commercial-scale algal biofuel production. We first investigated the potential of electrolytic water as a flocculant for harvesting Tetraselmis sp. Alkaline electrolyzed water (AEW) is produced at the cathode through water electrolysis. It contains mineral ions such as $Na^+$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$ that can act as flocculants. The flocculation activity with AEW was evaluated via culture density, AEW concentration, medium pH, settling time, and ionic strength analyses. The flocculation efficiency was 88.7% at 20% AEW (pH 8, 10 min) with a biomass concentration of 2 g/l. The initial biomass concentration and medium pH had significant influences on the flocculation activity of AEW. A viability test of flocculated microalgal cells was conducted using Evans blue stain, and the cells appeared intact. Furthermore, the growth rate of Tetraselmis sp. in recycled flocculation medium was similar to the growth rate in fresh F/2 medium. Our results suggested that AEW flocculation could be a very useful and affordable methodology for fresh biomass harvesting with environmentally friendly easy operation in part of the algal biofuel production process.

• Journal of Korean Society on Water Environment
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• v.33 no.5
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• pp.538-545
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• 2017

The purpose of this study is to investigate growth response and toxigenicity under various temperature and nutritional conditions, in order to understand the physioecological characteristics of Aphanizomenon flos-aquae, which is a bloom-forming cyanobacterium in the Nakdong River. The strain was inoculated into media under combinations of four temperatures (4, 12, 21, $30^{\circ}C$) and three nutrients (modified CB medium, P-depleted CB medium, N-depleted CB medium) for 28 days. The algae-inhibition tests were performed to assess the potential allelopathic effects of the strains' filtrates on the growth of four algae strains (Microcystis aeruginosa, Aulacoseria ambigua f. spiralis, Aphanizomenon flos-aquae, Scenedesmus obliquus). Toxin production of a strain was measured by Enzyme-Linked ImmunoSolbent Assay (ELISA). The optimal growth temperature (Topt) of strains was $19.9^{\circ}C$ ($18.3-21.2^{\circ}C$), and the temperature range for growth was from $-0.3^{\circ}C$ to $34.3^{\circ}C$. Specific growth rate (${\mu}$) in modified CB medium varied from 0.10 to $0.16day^{-1}$, and the maximum growth rate (${\mu}_{max}$) was $0.17day^{-1}$. Although growth curves under N-existed and N-depleted conditions were almost the same, growth under N-depleted condition was relatively slowed (${\mu}=0.09$ to $0.14day^{-1}$), with a decreased maximum cell density. However, growth under the P-depleted condition was restricted for all temperatures, Two stains of Aphanizomenon flos-aquae were confirmed as not producing toxins, because saxitoxin and cylindrospermopsin were not detected by ELISA. The exudates or filtrates from the Aphanizomenon flos-aquae (DGUC003) resulted in significant inhibition of algal growth on the Aulacoseira ambigua f. spiralis (DGUD001) and Aphanizomenon flos-aquae (DGUC001) (p < 0.01).

• Journal of Marine Bioscience and Biotechnology
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• v.7 no.1
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• pp.28-34
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• 2015

We examined cell regeneration efficiency of brown marine algae living in Jeju coast for search of a novel therapeutic device with cutaneous wound healing materials. The five algae were collected and compared with epidermal growth factor (EGF) as a positive control in the assays of cell proliferation and cell migration of NIH3T3 fibroblast cells. Among the 80% methanol extracts of these brown algae, the two algal extracts from Ishige foliacea and Colpomenia bullosa showed the proliferative effects of the cells similar to the effect of EGF. Besides it was found that Colpomenia bullosa extract significantly enhanced cell migration of NIH3T3 cell. In the study, therefore, we confirmed that the Colpomenia bullosa extract improved proliferation of NIH3T3 cell and a potential candidate for cultaneous wound healing.