Browse > Article
http://dx.doi.org/10.4014/mbl.1906.06005

Optimization for Scenedesmus obliquus Cultivation: the Effects of Temperature, Light Intensity and pH on Growth and Biochemical Composition  

Zhang, Yonggang (School of Chemical Science and Engineering, Tongji University)
Ren, Li (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University)
Chu, Huaqiang (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University)
Zhou, Xuefei (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University)
Yao, Tianming (School of Chemical Science and Engineering, Tongji University)
Zhang, Yalei (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.4, 2019 , pp. 614-620 More about this Journal
Abstract
Microalgae have been explored as potential host species for biofuel production. Environmental factors affect algal growth and cellular composition. The effects of several key environmental factors, such as temperature, light, and pH of the medium on the growth and biochemical composition of Scenedesmus obliquus were investigated in this study. The highest growth rate of microalgae was observed at an optimal temperature of 25℃, 150 μmol/(m2·s) light intensity, and pH 10.0. The biochemical composition analysis revealed that the carbohydrate content decreased at lower (20℃) or higher temperature (35℃), whereas the protein and lipid contents increase at these temperatures. The fluctuation of light intensity significantly affected the contents of protein, carbohydrate, and lipid. The protein levels varied greatly when the pH of the medium was below 7.0. The carbohydrate and lipid contents significantly increased at pH above 7.0.
Keywords
Scenedesmus obliquus; temperature; light; medium pH; growth rate; lipid content;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Qian KX, Borowitzka MA. 1993. Light and nitrogen deficiency effects on the growth and composition of Phaeodactylum tricornutum. Appl. Biochem. Biotechnol. 38: 93-103.   DOI
2 Hansen PJ. 2002. Effect of high pH on the growth and survival of marine phytoplankton : implications for species succession. Aquat. Microb. Ecol. 28: 279-288.   DOI
3 Zhu L, Hiltunen, E, Shu Q, Zhou W, Li Z, Wang Z. 2014. Biodiesel production from algae cultivated in winter with artificial wastewater through pH regulation by acetic acid. Appl. Energy 218: 103-110.
4 Gatamaneni BL, Orsat V, Lefsrud M. 2018. Factors affecting growth of various microalgal species. Environ. Eng. Sci. 35: https:// doi.org/10.1089/ees.2017.0521.
5 Ho SH, Chen CY, Chang JS. 20012. Chang Effect of light intensity and nitrogen starvation on $CO_2$ fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. Bioresour. Technol. 113: 244-252.   DOI
6 Gris B, Morosinotto T, Giacometti GM, Bertucco A, Sforza E. 2014. Cultivation of Scenedesmus obliquus in photobioreactors: effects of light intensities and light-dark cycles on growth, productivity, and biochemical composition. Appl. Biochem. Biotechnol. 172: 2377-2389.   DOI
7 Theses and Dissertations--Biosystems and Agricultural Engineering. Available from https://uknowledge.uky.edu/bae_etds/3. Accessed April 5, 2012.
8 Martinez ME, Jimenez JM, Yousfi FE. 1999. Influence of phosphorus concentration and temperature on growth and phosphorus uptake by the microalga Scenedesmus obliquus. Bioresour. Technol. 67: 233-240.   DOI
9 Sforza E, Gris B, de Farias Silva CE, Morosinotto T, Bertucco A. 2014. Effects of light on cultivation of Scenedesmus obliquus in batch and continuous flat plate photobioreactor. Chem. Eng. Trans. 38: 211-216.
10 Liu J, Yuan C, Hu G, Li F. 2012. Effects of light intensity on the growth and lipid accumulation of microalga Scenedesmus sp. 11-1 under nitrogen limitation. Appl. Biochem. Biotechnol. 166: 2127-2137.   DOI
11 Yang J, Li B, Zhang C, Hongxuan L, Zhou Y. 2016. pH-associated changes in induced colony formation and growth of Scenedesmus obliquus. Fundam. Appl. Limnol. 187: 241-246.   DOI
12 Allen MM. 1968. Simple conditions for growth of inicellular bluegreen algae on plates (1, 2). J. Phycol. 4: 1-4.   DOI
13 Ras M, Steyer JP, Bernard O. 2013. Temperature effect on microalgae: a crucial factor for outdoor production. Rev. Environ. Sci. Biotechnol. 12: 153-164.   DOI
14 Tang D, Han W, Li P, Miao X, Zhong J. 2011. $CO_2$ biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different $CO_2$ levels. Bioresour. Technol. 102: 3071-3076.   DOI
15 Chaplin MF, Kennedy JF. 1994. Carbohydrate analysis: a practical approach, 2nd Ed. pp. 1-41. IRL Press, New York.
16 Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 57: 911-917.   DOI
17 Nakamura Y, Miyach S. 1982. Effect of temperature on starch degradation in Chlorella vulgaris 11h cells. Plant Cell Physiol. 23: 333-341.
18 Williams PJLB, Laurens LML. 2010. Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics. Energy Environ. Sci. 3: 554-590.   DOI
19 Converti A, Casazza AA, Ortiz EY, Perego P, Borghi MD. 2009. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem. Eng. Process 48: 1146-1151.   DOI
20 Chang JS, Show PL, Ling TC, et al. 2016. Photobioreactors, pp. 313-352. In LarrIRL Pressoche C, Sanroman M, Du GC, Pandey A, Curr. Dev. Biotechnol. Bioeng, 1st Ed. Elsevier, Amsterdam.
21 Pandey SS, Kumar D, Tiwari BS. 2016. Chloroplast Metabolic Engineering for Sustainable Agriculture, pp. 149-162. In Dubey S, Pandey A, Sangwan R, Curr. Dev. Biotechnol. Bioeng, 1st Ed. Elsevier, Amsterdam.
22 Demirbas A. 2011. Competitive liquid biofuels from biomass. Appl. Energy 88: 17-28.   DOI
23 Abomohra AE, Eladel H, El-Esawi M, Wang S, Wang Q, He Z, et al. 2017. Effect of lipid-free microalgal biomass and waste glycerol on growth and lipid production of Scenedesmus obliquus: Innovative waste recycling for extraordinary lipid production. Bioresour. Technol. 249: 992-999.   DOI
24 Xin L, Hong-Ying H, Jia Y. 2010. Lipid accumulation and nutrient removal properties of a newly isolated freshwater microalga, Scenedesmus sp. LX1, growing in secondary effluent. N. Biotechnol. 27: 59-63.   DOI
25 Scott SA, Davey MP, Dennis JS, Horst I, Howe CJ, Lea-Smith DJ, et al. 2010. Biodiesel from algae: challenges and prospects. Curr. Opin. Biotechnol. 21: 277-286.   DOI
26 Koller M, Salerno A, Tuffner P, Koinigg M, Bochzelt H, Schober S, et al. 2012. Characteristics and potential of micro algal cultivation strategies: a review. J. Clean Prod. 37: 377-388.   DOI
27 Malcata FX. 2011. Microalgae and biofuels: a promising partnership? Trends Biotechnol. 29: 542-549.   DOI
28 Behrens PW, Kyle DJ. 2010. Microalgae as a source of fatty acids. J. Food Lipids 3: 259-272.   DOI
29 Juneja A, Ceballos RM, Murthy GS. 2013. Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: a review. Energies 6: 4607-4638.   DOI
30 Cabello J, Toledo-Cervantes A, Sánchez L, Revah S, Morales M. 2015. Effect of the temperature, pH and irradiance on the photosynthetic activity by Scenedesmus obtusiusculus under nitrogen replete and deplete conditions. Bioresour. Technol. 181: 128-135.   DOI
31 Fan J, Huang J, Li Y, Han F, Wang J, Li X, et al. 2012. Sequential heterotrophy-dilution-photoinduction cultivation for efficient microalgal biomass and lipid production. Bioresour. Technol. 112: 206-211.   DOI