• 한국작물학회지
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• 제34권1호
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• pp.48-54
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• 1989

외국에서 도입한 32개의 서로 다른 Alfalfa 품종을 우리나라 기후풍토에서 재배하여 초장과 건물중 등의 생육상태를 비교 검사한 결과 가장 우수한 생육상태를 나타낸 품종들은 다음과 같다. 1. 초장생육이 우수하였던 상위 7개 품종들은 Diamond>Milkmaker> Arrow>Chalienger>Sapphire > Dekalb brand 135> LS-1920의 품종순이고 최하위 초장의 7개 품종을 하위순으로 열거하여 보면 Pioneer brand 5929> Maxidor> Dekalb brand 167> Pierce> Blazer> Raidor> Pioneer brand 532의 순이었다. 2. 건물중이 많은 상위 7개 품종은 Pike>LS-1920>Challenger>Salute>Diamond>Decathlon>Pioneer brand 581의 품종순이며 최하위 건물중의 7개 품종은 Maxidor>Pioneer brand 5929>Pioneer brand 532>Florida 77> Pierce> Magnum> Blazer의 순이었다. 3. 초장과 건물중의 결과를 비교하여 보면 대체로 초장에서 우수하였거나 열거하였던 품종들이 역시 건물중에서도 우세하거나 열거하여 서로가 비례하는 결과를 보였다. 4. 이상에서 조사한 바와 같이 가장 좋은 생육상태 및 수활량을 보인 품종들은 Pike, LS-1920, Challenger, Salute, Diamond 등이었으며 이들중 Challenger 품중을 제외한 나머지 품종들은 내한성이 중간정도인 Semi-dormant 계통으로 우리 나라 재배환경에 가장 적합할 것으로 생각된다.

• 한국초지조사료학회지
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• 제10권3호
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• pp.141-146
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• 1990

Changes in dry matter yield, crude components, nutrients uptake($P_2O_5$, $K_2O$, CaO, MgO) and sulphur containing amino acid(cysteine, methionine) of orchardgrass(Dacty1is glomerata. L) and alfalfa(A4edicago sativa. L) by gypsum application(as sulphur source, 0, 2. 5, 10, 20kg SIlOa) were investigated to understand the effect of sulphur on herbage production in pasture, which was established in 1987 as means of hand broadcasting. The effect of gypsum on dry matter yield at different cutting times during growing seasons has not been found both in orchardgrass and in alfalfa, but in respect to annual total dry matter yield there were increment in herbage yield (P<0.05) of alfalfa at 5, 10, 20kg SIlOa in 1989 and the amounts of sulphur taken up in herbage slightly increased according to the rates of gypsum application. Maximum apparent recovery of sulphur was 7.55% at 2kg SIlOa in orchardgrass and was 17.8% at 5kg S/lOa in alfalfa. There were no any great differences in the content of crude components of both species and this trend was similar with the mineral contents of orchardgrass. But in alfalfa, the amounts of $K_2O$, CaO, and $P_2O_5$ taken up were increased by gypsum application and the increment in the amounts of minerals taken up in herbage at 20kg SIlOa were 14.9 of $K_2O$, 9.1 of CaO, and 2.5kgIlOa of $P_2O_5$ as compared to those of at untreated plot. Cysteine and methionine were not influenced by gypsum applicaton not only in orchardgrass but also cysteine in alfalfa, however, the content of methionine in alfalfa was slightly increased at 2, 5, lOkg SIlOa and at 20kg SIlOa was reverse.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1098-1113
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• 2021

Maturation process of alfalfa (Medicago sativa L.) could be prevented by the reduction of lignin content in terms of conventional breeding or transgenic technology. Alfalfa could exhibit higher leaf/stem ratio, with a concern of yield loss. The objective of this study was to compare forage yield and nutritive value of low lignin alfalfa and two reference varieties subjecting to two harvest intervals and three seeding rates. The experimental design was a randomized complete block in a split-split plot arrangement with four replicates, where harvest intervals (28-day and 35-day) were assigned to whole plots, seeding rates were subplots, and varieties were sub-subplots. The weighted mean nutritive value was applied to two production years of 2016 and 2017. Hi-Gest 360 (low lignin alfalfa) provided similar yield potential and increased nutritive value compared to two reference varieties. Over a two-year production period, alfalfa harvested at every 28-day interval provided more economic returns than those at 35-day interval. For the seeding year and first production year, five cuts made by the 28-day interval produced more yield than four cuts by the 35-day interval. Due to limited rainfall in May 2017, a sharp drop of the first cutting overturned the advantage of the five-cut system. Shorter intervals between harvests generally increased crude protein (CP) concentrations. The differences of relative feed value (RFV) between two harvest intervals tended to be great during the first and second cuttings. Overall, harvest interval had a large effect on nutritive value and a more significant effect on alfalfa dry matter yield than variety selection. Seeding rate did not affect alfalfa yield and nutritive value.

• 한국초지조사료학회지
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• 제41권3호
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• pp.162-167
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• 2021

Aluminum (Al) stress in acidic pH is known to decrease the growth and productivity of alfalfa. However, not much is known about how the application of silicon (Si) affects the Al stress response in alfalfa. This study was conducted to evaluate the effect of exogenous application of Si on the growth of alfalfa seedlings exposed to Al stress in pots. Alfalfa seedlings grown in pots for 2 weeks were treated either Al stress (pH 4.0, 0.2 mM Al) or Al stress + Si (1 mM) for 5 days, lengths and biomass of shoot and root, and chlorophyll and carotenoid contents in leaf tissues were analyzed respectively. Al stress treatment inhibited shoot and root growth, and decreased fresh and dry weights, and chlorophyll content in leaves, but increased carotenoid content. In contrast, when alfalfa seedlings treated with Al stress combined with Si, delayed growth caused by Al stress of shoot and root of alfalfa seedlings was restored, dry weight was increased and chlorophyll content of leaf tissue was increased, but carotenoid content was decreased. These results suggest that Si has a function of alleviating Al toxicity in alfalfa, of which it exhibits a mitigating effect by a function that overlaps with some of the intracellular functions of carotenoids.

• 한국초지조사료학회지
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• 제42권3호
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• pp.169-175
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• 2022

This study aimed to examine the changes in dry matter yield and growth characteristics of alfalfa (Medicago sativa L.) in response to variations in sowing dates during the autumn season of 2021-22 in a dry paddy field of Chilbo-myeon, Jeongeup-si, Jeollabuk-do. Treatments comprised four sowing dates at 10-day intervals, i.e., October 8, October 18, October 28, and November 8, 2021. The winter survival rate of alfalfa showed a significant difference between different treatments but was at a satisfactory level for all (p<0.05). The winter survival rate for the fourth sowing date, a month later than the first sowing date, was approximately 11.7% lower than that for the first sowing date. The plant height ranged between 82.3-93.1 cm and 60.5-63.7 cm at the first and second harvest, respectively, smaller at the second harvest than at the first harvest. The total dry matter yield of alfalfa was the highest at 13,316 kg/ha for the first sowing date, and the later the sowing date, the lower the dry matter yield. The protein content of alfalfa ranged between 13.6-17.3% in the first harvest, lower than the standard alfalfa protein content of 20% or more. In relative feed value, the first sowing (Oct. 8) was the most significantly higher in the first harvest (p<0.05). These results suggest that the early and mid-October sowing dates are optimum for sowing alfalfa during autumn and result in improved plant growth, dry matter yield, protein content, and winter survival compared to those at later sowing dates. Therefore, dry paddy fields can be safely employed for alfalfa cultivation with sowing dates in early and mid-October during autumn.

• 한국초지조사료학회지
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• 제4권1호
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• pp.28-34
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• 1983

alfalfa 단파초지(單播草地)에 있어서 최대총건물(最大總乾物) 생산(生産)과 질적(質的) 향상(向上)을 위한 예취적기(刈取適期)가 연구(硏究)되었다. 최대총생산수량(最大總生産收量)을 위한 예취기(刈取期) tc(장(張), 1971)가 alfalfa 초종(草種)의 영속성(永續性)과 동시에 CP 및 IVD의 높은 수준(水準)을 수분하는 최대건물생산수량(最大乾物生産收量)의 적기(適期)로 선택(選擇)되었다. 이와 동일(同一)한 이론적(理論的) 근거에 입각하여 극대(極大) LAI 및 25% 개화기(開花期)가 alfalfa 단파초지(單播草地)에서 가장 적합(適合)한 형태학적(形態學的) 예취기(刈取期)임이 판명(判明)되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제1권1호
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• pp.1-5
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• 1988

Ninety feeder steers, predominantly Herefords weighing 280 kg, were assigned at random to each of nine diets: basal (high concentrate); 25 or 50% of untreated rice straw (25- or 50 URS) or ammoniated rice straw (25- or 50 ARS); a 50:50 mixture of URS - alfalfa or ARS - alfalfa replacing a proportion of the basal at 25 and 50%. Animals were slaughtered after 154 or 161 days of feeding. The cattle fed 50% URS had the lowest (P<.05) carcass weight, dressing & external fat thickness, kidney, pelvic and heart fat, rib eye area, marbling score, quality and yield grades, followed by cattle fed 50% ARS, 50% rice straw/alfalfa, and 25% rice straw alone or 25% rice straw/alfalfa mixture. Ammoniation of rice straw improved all measured traits. A 25% substitution of basal diet with untreated or ammoniated rice straw and a 50% substitution with rice straw/alfalfa mixture did not significantly affect carcass traits compared to the basal group. Only 50% ARS and 50% URS cattle showed differences in body composition (P<.05) with lower fat and higher water and protein contents. No significant differences were found in shear value, panel tenderness, connective tissue, juiciness, flavor and overall palatability of meat from steers fed the basal, ARS or URS diets.

• 원예과학기술지
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• 제32권6호
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• pp.901-905
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• 2014

The efficacy of ozone micro-bubble water (OMBW) in reducing microbial populations on alfalfa seeds was investigated in this study. We observed the surface of alfalfa seeds using microscopy and found that many cracks and crevices existing on the surface could harbor pathogens. Alfalfa seeds were treated with tap water (TW), micro-bubble water (MBW), ozone water (OW), ozone micro-bubble water (OMBW), and chlorine water (CL) for 5 min, and total microbial population, E. coli and Salmonella spp. colonies were determined. Also, the sterilized seeds were germinated and cultivated for 5 d after sowing to investigate the percentage of germination and the growth of alfalfa sprouts. The treatments with OMBW and CL were most effective in reducing total microbial populations and E. coli was eliminated by OW, OMBW, and CL treatments. CL treatment reduced the percentage of germination and fresh weight of alfalfa sprouts, but OMBW did not cause any negative effects on the germination and growth of alfalfa sprouts. These results indicate that OMBW can be used as an effective sanitizer for eliminating seed-borne pathogens without detrimental effects on seed viability.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2007년도 학술발표회 논문집
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• pp.1423-1426
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• 2007

The goal of this research is to develop and apply the integrational model for the pan evaporation and the alfalfa reference evapotranspiration in Republic of Korea. Since the observed data of the alfalfa reference evapotranspiration using lysimeter have not been measured for a long time in Republic of Korea, PM method is used to assume and estimate the observed alfalfa reference evapotranspiration. The integrational model consists of staochastics and neural networks processes respectively. The stochastics process is applied to extend for the short-term monthly pan evaporation and alfalfa reference evapotranspiration. The extended data of the monthly pan evaporation and alfalfa reference evapotranspiration is used to evaluate for the training performance. For the neural networks process, the generalized regression neural networks model(GRNNM) is applied to evaluate for the testing performance using the observed data respectively. From this research, we evaluate the impact of the limited climatical variables on the accuracy of the integrational operation of stochastics and neural networks processes. We should, furthermore, construct the credible data of the pan evaporation and the alfalfa reference evapotranspiration, and suggest the reference data for irrigation and drainage networks system in Republic of Korea.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제1권1호
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• pp.83-89
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• 2020

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.