• 대한통합의학회지
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• 제4권4호
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• pp.109-117
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• 2016

Purpose : This study was designed to provide basic data of effective sanitization method of sprout vegetable for microbiological safety. Methods : Sanitization treatments were performed by dipping the sprout into chlorine and vinegar water. Microbial analysis were composed of the total plate count(TPC) and coliform group count(CGC). Result : Among chlorine water sanitization, the microbial reduction was largest in 100ppm chlorine water, and its TPC and coliform group count decreased to 6.01 log CFU/g and 5.06 log CFU/g. The effective dipping time in 100ppm chlorine water treatment was 5.97 log CFU/g and 5.91 log CFU/g for 30min and 60min, in which TPC were below the microbiological safety limits of 6.00 log CFU/g. Coliform group counts were decreased to 5.44, 5.46, 5.42 log CFU/g in the dipping the spouts for 30min, 60min and 90min. As a result of sanitizing alfalfa spout by vinegar water, a microbial counts tended to decrease with increasing concentration and the dipping time. The effective concentration of vinegar water was 2% for TPC(6.00 log CFU/g) and 1% for coliform group count(5.20 log CFU/g). With 1% vinegar water treatment, TPC became below the microbiological safety limits in all samples and in particular, the sample treated for 60min and 90min(4.93, 4.54 log CFU/g). While coliform group counts were decreased to 3.91 log CFU/g in the dipping the sprouts for 90min, those were still beyond the permitted limit. Conclusion : To secure the food safety of food service facilities including kindergarten feeding, it is considered that along with the systematic study of effective disinfection method for microbiological control at the preconditioning level of spout vegetable within the range of secured maleficence to human body, and the study regarding the measures to lower the initial microbiological pollution of spout vegetable.

• 한국작물학회지
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• 제41권5호
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• pp.542-550
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• 1996

알팔파 (Medicago sativa L.)의 예취후 재생 기간중 저장탄수화물의 이용성을 규명하기 위해 수경재배하여 개화초기에 예취한 후 재생 24일간의 뿌리내 비구조탄수화물의 함량 및 전분 분해효소의 활력을 분석한 결과는 아래와 같다. 1. 재생초기 10일간의 잎과 줄기의 재생은 매우 느리게 진행되었으며, 예취후 뿌리의 성장이 억제되었다. 2. 예취후 초기재생 10∼14일간 뿌리내 가용성 당 및 전분의 함량은 다같이 감소하였다가 이후 빠르게 회복하는 경향이었다. 3. 재생기간중 exo-amylase의 평균 활력은endo-amylase에 비해 약 400배 이상 높았다. Exo-amylase의 활력은 재생 6일차(최고수준) 까지 증가하다가 이후 감소하였다. Endo-amylase의 활력은 재생초기 4일 동안 급격히 증가하다가 이후 재생 24일차(최고수준) 까지 서서히 증가하는 경향이었다. 이상의 결과들은 알팔파의 재생초기 동안 전분 분해효소의 활력의 증가와 아울러 뿌리내 저장탄수화물은 활발히 분해되어 새로운 조직의 재생에 이 용됨을 간접적으로 제시한다.

• 한국초지조사료학회지
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• 제17권4호
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• pp.371-378
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• 1997

The purpose of this research was to investigate the effect of P fertilization and cutting fkquency without N fertilizer on nutritive value and nitrogen fixation activity of alfalfa. A field experiment was conducted on the experimental farm of Kongju National University at Yesan from 1994 to 1996. The experimental design was a split plot design with two P levels as main plot and two cutting frequencies as the sub-plot. The results obtained are summarized as follows. Available phosphate, exchangeable Ca and Na in the soil were slightly increased with P fertilizer and basal treatment of lime. Average DM yield was 1.5 ton and 1.4 ton in second and third year. There was no significant difference within P level and cutting kquency. The first cut showed higest CP value as cutting times increased the value tend to be lowered. Crude protein content in 3 and 4 cutting regimes were from 16.3 to 16.6% 17.4 to 17.9% respectively, but no significant effect were found among the main and sub-plot. ADF and NDF in 1995 were ranged from 38.2 to 39.9, 45.1 to 58.1% respectively. ADF was 2% more higher in 1996 compared to previous year and NDF was slightly higher in 1996. There was significant difference of ADF between main plot in 1995. .P, Ca, K and Mg of 200kg P level with 3 times cutting treatment in 1995 and 1996 were 0.32 and 0.23, 0.95 and 0.84, 3.24 and 2.87, and 0.36 and 0.26. Significant difference of K and Mg between cutting frequency was observed in 1995. Nitrogen fixing activity of 400kg P fertilizer plots were higher than 200kg P level and 3 times cutting plot also showed higher acetylene reduction activity than that of 4 cutting per year. Phosphate level and cutting frequency treatment of did not significantly affect nitrogen fixation activity.

• 한국초지조사료학회지
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• 제9권3호
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• pp.124-128
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• 1989

本實驗은 Vernal알팔파의 callus 誘導에 미치는 몇가지 要因들에 對하여 糾明하고자 逐行되었으며 얻어진 結果는 다음과 같다, Callus 誘導에서 auxin 源으로서 2,4-D를 2-5mg/1 單用處理한 것이 가장 좋았으며, 여러가지 基本 培地 中에서는 B5와 SH가 callus 誘導에 가장 效果的이었는데, PC와 MS 培地에서 生成된 callus는 friable 하였다. 培地#의 pH는 5.8이 가장 좋았으며 7.0 以上에서는 callus는 거의 생장하지 않았다. 組織片은 9日 齡된 植物體의 것이 가장 좋았으며, 이를 前後하여 callus의 生成量은 減少하였다. Callus 誘導時, 光條件의 有無는 큰 影響을 나타내지 않았으나, 빛 條件에서는 callus 組織은 푸르게 되었다.

• 한국농공학회지
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• 제16권4호
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• pp.3555-3571
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• 1974

This experiment of which aim contribute to plan irrigation system so as to increase forage crop yields, was conducted to estimate evapotranspiration amount of forage crops and to find out system of consumed water in a pasture-ground. The results obtained by this study are as follows: 1. The general weather conditions which, were closely related to the evapotrannpiration of forage crops were nearly same as those of the average year with the exception that temperature of May and June were slightly low. 2. According to the investigation of potential evapotranspirations (P.E) or forage crops and its changes during growing periods, changes of tenday P.E. were high significant according to the harvesting period. P.E of Alfalfa of which yield was the largest was the biggest. Althrough the correlations between P.E. and meteorological factors were irregular oming to three-time harvesting, correlation between ten-day evapotraspiration amount and copper plated pan evaporation or solar radiation was high positive significant. 4. Predicting formulas of P.E. were led by weather factors, and also relatione between P.E. and weather factors were showed as figure. from the these formulas, P.E. may be calculated by weather factors. 5. Predicting formulas of P.E. were led by mean temperature and copper plated pan evaporation, and by mean temperature and solar radiation. As computed values and measured values showed in figure, these formulas were high signiflent. 6. In the total consumed soil water duration of 10 days which, was non-rain period from 12th to 21th of August, Alfalfa was the largest 48.1mm, second, Orchard grass 40.1mm and Fescue 37.6mm, and Ladino clover was the smallest 37.1mm, also, order of each forage crop yield amound. was same to the abov. Order of soil moisture extraction rate of soil layer of all the for forage crops dulation of ten-day was soil layer 1 which was largest, soil layer 2, 3, and 4 Reviewing the the first five-day and the second five day, in the first five-day, order of that of all the forage crops was same to the above, but in the second five-day, that of soil layer 2 or 3 was more than the of soil layer 1.

• 한국초지조사료학회지
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• 제12권3호
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• pp.153-160
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• 1992

본 시험은 고령지 두과목초의 적응성을 구명하기 위하여 표고가 다른 3개지역(600,800,1000m a.s.l)에서 주구를 파종시기(추파.추파), 세구를 혼파조명(알팔파-화본과, 레드크로바-화본과, 화이트크로바-화본과)으로 두고 분할구 배치 3반복으로 수행하였다. 1.600m에서 두과목초의 적응성을 보면 알팔파, 레드클러버 및 화이트클로버 공히 추파를 했을 때 연차간 악화없이 약 30%를 유지하여 적응성이 매우 양호하였다.2. 800m에서는 화치트클로버를 춘파하므로써 연차간에 심한 감소없이 최종 예취시 약 14.0%를 점유하고 있었으며 또한 10a당 건물수량이 가장 많았다. 3. 1000m에서도 800m와 비슷한 경향으로 화이트클로버를 춘파했을 경우 어느정도 적응이 가능하였다. 4. 표고별 두과목초의 비솔 및 수량이 표고가 증가 할수록 현저하게 감소하였으며 알팔파는 800m 이상에서는 생육이 거의 불가능하였다.

• 한국유기농업학회지
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• 제28권4호
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• pp.605-614
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• 2020

This study was conducted to investigate the effects of TMR on in vitro rumen fermentation and methane production of goat with different forage sources. The experiment was arranged 4×2 factorial design. The different forage sources were rice straw (RS), Italian rye grass (IR), timothy (TI) and alfalfa (AL), respectively. There were two different forage : concentrate ratios such as 20:80 (20) and 50:50 (50), respectively. Therefore, totally 8 treatments were used: 1) RS20, 2) RS50, 3) IR20, 4) IR50, 5) TI20, 6) TI50, 7) AL20, and 8) AL50, respectively. The rumen fluid of goat was collected from the slaughterhouse. For fermentation parameters, ruminal pH, total gas, methane, hydrogen, ammonia nitrogen, and volatile fatty acid were determined. The pH values were within an optimal range across all treatments. Total gas productions at TI20 and AL50 were significantly greater than others (p<0.05). Methane production was significantly lower in TI and AL compared with other treatments (p<0.05). The relatively high dietary NDF content in treatments showed significantly lower methane production (p<0.05). Significant alterations treatments were detected at ammonia nitrogen concentration according to the ratio of forage : concentrate (p<0.05). AL treatment showed greater total volatile fatty acid production compared with other treatments (p<0.05). Therefore, the present study suggests that both Timothy and Alfalfa could be recommendable forage sources for goat based on results with volatile fatty acid as an energy source and methane as an index for energy loss and environmental issues. Also, the 50:50 (forage : concentrate) ratio would prefer to 20:80.

• The Plant Pathology Journal
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• 제37권6호
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• pp.619-631
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• 2021

Alfalfa mosaic virus (AMV), an economically important pathogen, is present worldwide with a very wide host range. This work reports for the first time the infection of Vinca minor and Wisteria sinensis with AMV using RNA sequencing and reverse transcription polymerase chain reaction confirmation. De novo assembly and annotating of contigs revealed that RNA1, RNA2, and RNA3 genomic fragments consist of 3,690, 2,636, and 2,057 nucleotides (nt) for IR-VM and 3,690, 2,594, and 2,057 nt for IR-WS. RNA1 and RNA3 segments of IR-VM and IR-WS closely resembled those of the Chinese isolate HZ, with 99.23-99.26% and 98.04-98.09% nt identity, respectively. Their RNA2 resembled that of Canadian isolate CaM and American isolate OH-2-2017, with 97.96-98.07% nt identity. The P2 gene revealed more nucleotide diversity compared with other genes. Genes in the AMV genome were under dominant negative selection during evolution, and the P1 and coat protein (CP) proteins were subject to the strongest and weakest purifying selection, respectively. In the population genetic analysis based on the CP gene sequences, all 107 AMV isolates fell into two main clades (A, B) and isolates of clade A were further divided into three groups with significant subpopulation differentiation. The results indicated moderate genetic variation within and no clear geographic or genetic structure between the studied populations, implying moderate gene flow can play an important role in differentiation and distribution of genetic diversity among populations. Several factors have shaped the genetic structure and diversity of AMV: selection, recombination/reassortment, gene flow, and random processes such as founder effects.

• 한국수자원학회논문집
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• 제55권10호
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• pp.801-810
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• 2022

본 연구에서는 홍수터 식생에 의한 하안의 안정성을 고려하여 하도에서 사주의 거동과 하도의 지형변화 적응과정을 실내실험을 수행하여 분석하였다. 초기 직류하도에서 하안침식이 발생하고 하폭이 증가한다. 하폭 대 수심의 비가 증가한 후에, 교호사주가 발달하고, 하류로 이동한다. 시간이 증가함에 따라, 사주의 이동속도는 감소하고 사주의 파장은 증가한다. 홍수터에서 식생 밀도가 증가함에 따라, 하안침식율은 감소한다. 그러나 사주의 이동속도는 증가한다. 이는 하안의 안정성 변화가 사주의 이동속도에 영향을 주기 때문이다. 하폭이 증가하고 수심이 감소하면서 무차원 소류력인 Shield Number는 증가하며, 사주의 이동속도도 증가한다. 이는 또한 이동하는 교호사주와 선택적 침식에 의해 발생한 불규칙한 하안 사이에 작용하는 강제효과 때문에 하안의 안정성이 작은 하도에서 사주의 이동속도는 감소한다. 시간이 증가함에 따라 사주의 파장은 증가한다. 사주의 파장이 증가하면서 사주의 이동속도는 감소한다.

• 한국작물학회지
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• 제27권2호
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• pp.137-140
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• 1982

두과목초의 근류균 접종방법 개선책의 일환으로써 3수준의 근류균농도, 즉 10, 20, 30%; 3종류의 접착제 즉 gum arabic, methyl cellulose 및 carboxy methyl cellulose; 그리고 4종류의 석회와 calcium carbonate를 포함한 5종류의 피복재료를 사용하여 근류균을 접종한 후에 plate count법으로 생존하고 있던 근류세포수를 측정하여 그 효과를 평가하였던 바 다음과 같은 결론을 얻었다. 1. New Zealand산 peat slurry의 근류균을 사용할 경우 근류균을 10%에서 3.5$\times$$10^9$ 개로써 표준에 가까운 근류균수를 확보할 수 있었다. 2. 접착제로서 gum arabic은 40% 용액에서 methyl cellulose는 5% 용액 그리고 carboxy methyl cellulose는 4% 용액으로 사용하였을 때 최대착생수를 나타내었으나 그 중에서 methyl cellulose 5% 용액이 적합한 것으로 보였다. 3. 피복재료로서 치과용석회(Lime A)는 타재료에 비하여 양호한 결과를 얻을 수 있었다.