• 한국작물학회지
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• 제49권1호
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• pp.30-35
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• 2004

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous extracts from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine their allelopathic effects, and the result showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Untreated seeds germinated in 60h, but extract concentrations greater than 30g $\textrm{L}^{-1}$ delayed seed germination. The extracts significantly inhibited seed germination of alfalfa, and $\beta$-amylase activity of alfalfa and barley seeds during 24-36 hours after treatment. Aqueous extracts of 40 g $\textrm{L}^{-1}$ from X. occidentale were completely inhibited the hypocotyl and root growth of alfalfa. Aqueous leaf extracts showed the highest inhibitory effect and followed by root and stem extracts. Early seedling growth of both alfalfa and barnyard grass (Echinochloa crus-galli) was significantly reduced by methanol extracts. By means of high-performance liquid chromatography, chlorogenic acid and trans-cinnamic acid were quantified as the highest amounts from water and EtOAc fractions, respectively. BuOH and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did hexane and water fractions. The findings of the bioassays for aqueous or methanol extracts reflected that the inhibitory effect of extract was closely related to the level of responsible allelochemicals found in plant extracts.

• 한국초지조사료학회지
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• 제42권3호
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• pp.137-145
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• 2022

Aluminum (Al) is one of the major factors adversely affects crop growth and productivity in acidic soils. In this study, the effect of Al on plants in soil was investigated by comparing the protein expression profiles of alfalfa roots exposed to Al stress treatment. Two-week-old alfalfa seedlings were exposed to Al stress treatment at pH 4.0. Total protein was extracted from alfalfa root tissue and analyzed by two-dimensional gel electrophoresis combined with MALDI-TOF/TOF mass spectrometry. A total of 45 proteins differentially expressed in Al stress-treated alfalfa root tissues were identified, of which 28 were up-regulated and 17 were down-regulated. Of the differentially expressed proteins, 7 representative proteins were further confirmed for transcript accumulation by RT-PCR analysis. The identified proteins were involved in several functional categories including disease/defense (24%), energy (22%), protein destination (9%), metabolism (7%), transcription (5%), secondary metabolism (4%), and ambiguous classification (29%). The identification of key candidate genes induced by Al in alfalfa roots will be useful to elucidate the molecular mechanisms of Al stress tolerance in alfalfa plants.

• 한국초지조사료학회지
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• 제17권3호
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• pp.239-248
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• 1997

This study was conducted to investigate the effects of antagonistic microorganisms, Bacillus spp., on growth of alfalfa(Medicag0 sativa L.) in repeated cultivation soil(RCS) and unrepeated cultivation soil(URCS). Alfalfa was established by seeding into pots 12 cm in diameter and 9 cm in depth containing 1 : 1 mixture of soil and vermiculite with antagonistic bacteria and pathogenic fungi. The growth experiment of alfalfa was conducted in pots in a vinyl house. The bacteria used in this study were Bacillus subtilis and hsants. B. subtilis was isolated and identified 60m forage rhizosphere soil and hsants isolated through cell fusion fiom B. subtilis 101 and B. thuringiensis. B. subtilis was named B. subtilis 101 and hsants named F -3 and F -8. From dark culture experimenf alfalfa was longer lived in treated soil with antagonistic bacteria than that in non-treated soil, and longer lived in URCS than that in RCS. However, alfalfa was shorter lived in RCS and URCS than that in autoclaved RCS. The number of leaves of alfalfa were positively affected by the inoculation of the antagonistic bacteria in both RCS and URCS. Dry weight of shoot and root was increased by the inoculation of the antagonistic bacteria(P< 0.05). However, the growth of alfalfa was decreased by the inoculation of the pathogenic hngi both RCS apd URCS.

• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1521-1527
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• 2019

Objective: To study the contribution of plant enzyme and microbial activities on protein degradation in silage, this study evaluated the nitrogen transformation dynamics during ensiling of non- and irradiated alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.). Methods: Alfalfa and red clover silages were prepared and equally divided into two groups. One group was exposed to ${\gamma}$-irradiation at a recommended dosage (25 Gky). Therefore, four types of silages were produced: i) non-irradiated alfalfa silage; ii) irradiated alfalfa silage; iii) non-irradiated red clover silage; and iv) irradiated red clover silage. These silages were opened for fermentation quality and nitrogen components analyses after 1, 4, 8, and 30 days, respectively. Results: The ${\gamma}$-irradiation successfully suppressed microbial activity, indicated by high pH and no apparent increases in fermentation end products in irradiated silages. All nitrogen components, except for peptide-N, increased throughout the ensiling process. Proteolysis less occurred in red clover silages compared with alfalfa silages, indicated by smaller (p<0.05) increment in peptide-N and free amino acid N (FAA-N) during early stage of ensiling. The ${\gamma}$-irradiation treatment increased (p<0.05) peptide-N and FAA-N in alfalfa silage at day 1, whereas not in red clover silage; these two nitrogen components were higher (p<0.05) between day 4 and day 30 in non-irradiated silages than the irradiated silages. The ammonia nitrogen and non-protein nitrogen were highest in non-irradiated alfalfa silage and lowest in irradiated red clover silage after ensiling. Conclusion: The result of this study indicate that red clover and alfalfa are two forages varying in their nitrogen transformation patterns, especially during early stages of ensiling. Microbial activity plays a certain role in the proteolysis and seems little affected by the presence of polyphenol oxidase in red clover compared with alfalfaa.

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.758-765
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• 1999

Comparative studies of the effects of rates and frequency of application of dairy slurry on herbage yield and stand persistence of alfalfa and various forage grasses have not previously been conducted. The results being reported here are part of a larger study having a primary objective of comparing the effectiveness of alfalfa (Medicago sativa L.), various grasses and alfalfa-grass mixtures for utilizing nutrients from applied dairy slurry. The objectives of this part of the study were to evaluate the effects of various rates and frequencies of application of slurry on herbage yield and stand persistence of orchardgrass (Dactylis glomerata L.), reed canarygrass (Phalaris arundinacea L.), and alfalfa-orchanrdgreass and alfalfa-reed canarygrass mixtures managed as a 4-cutting management system. A randomized complete block design with treatments in a split plot arrangement with four replicates was used. The main plots consisted of 9 fertility treatments: 7 slurry rate and time of application treatments, one inorganic fertilizer treatment, and an unfertilized control. The sub-plots consisted of the two grasses and two alfalfa-grass mixture mentioned above. Slurry was composed from stored solids scraped from the alleyways of a free-stall housing barn and water added to form a slurry having about 8% solids. Manure was pumped from a liquid spreader tank into 10.4 L garden water cans for manual application to the plots. Herbage yields within species were generally unaffected by various rates of application in the first production year. Herbage yields of grasses and alfalfa-grass mixtures the second year were generally not affected by frequency of application for the same rate of slurry applied. Slurry application resulted in greater herbage yield increases in grasses than alfalfa-grass mixtures in the 4-cutting management system. In general, herbage dry matter yields of grasses from the dairy slurry treatments equaled or exceeded yields from the inorganic fertilizer treatment. Stand ratings of grasses and alfalfa-grass mixtures were not changed by manure application rates. In this study, the highest rate of slurry ($967kg\;total\;N\;ha^{-1}$ in 1995 plus $2,014kg\;N\;ha^{-1}$ in 1996) was not detrimental to herbage yields or stand persistence of any of the species. It was concluded that applying dairy slurry to these cool-season grasses and alfalfa-grass mixtures managed in a 4-cutting system is an acceptable practice from the standpoint of herbage yield and satnd persistence and by doing so the utilization of inorganic fertilizers can be reduced.

• Asian-Australasian Journal of Animal Sciences
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• 제22권3호
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• pp.371-377
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• 2009

The objectives of the present study were to investigate the effects of different dietary ratios of whole-crop corn silage and alfalfa hay on nitrogen (N) digestion, duodenal flow and metabolism across the portal-drained viscera (PDV) of growing beef steers, and to elucidate their relationships. Four steers (236${\pm}$7 kg BW) fitted with duodenal cannulae and chronic indwelling catheters into the portal and mesenteric veins and abdominal aorta were used in a 4${\times}$4 Latin square design. Animals were fed (at 12-h intervals) the 4 diets consisting of whole-crop corn silage (C) and alfalfa hay (A) in 80:20 (C8A2), 60:40 (C6A4), 40:60 (C4A6) and 20:80 (C2A8) ratios of which dietary crude protein (CP) was 10.5, 12.0, 13.5 and 15.0% of dry matter (DM), respectively. Feeding level was restricted to 95% of ad libitum intake to measure N digestion, blood flow and net flux of N across the PDV. Digestibility of DM and neutral detergent fiber and digestible energy intake linearly increased as the ratio of alfalfa hay increased. The N intake, duodenal flow and intestinal disappearance increased linearly with increasing alfalfa hay. Arterial and portal concentrations of ${\alpha}$-amino N showed a quadratic response to increasing levels of alfalfa hay and were the highest in steers fed the C6A4 diet. The net PDV release of ${\alpha}$-amino N and ammonia N increased linearly with increasing alfalfa hay, but urea N uptake by PDV did not differ among diets. As a percentage of apparently digested N in the total gut, net PDV release of ${\alpha}$-amino N linearly decreased from 66 to 48% with increasing alfalfa hay. Conversely, net PDV recovery of ${\alpha}$-amino N to intestinal N disappearance varied with increasing alfalfa hay accounting for 49, 50, 58 and 61% on C8A2, C6A4, C4A6 and C2A8 diets, respectively. Net PDV uptake of urea N, relative to apparently digested N, linearly decreased from 81 to 25% as alfalfa hay increased from 20 to 80% of DM intake. Considering PDV uptake of urea N, microbial efficiency and conversion of total tract digested N to PDV ${\alpha}$-amino N net supply, a diet consisting of 80% whole-crop corn silage and 20% alfalfa hay (10.5% CP) was the best, while considering the quantities of intestinal N disappearance and ${\alpha}$-amino N absorption, a diet of 20% whole-crop corn silage and 80% alfalfa hay (15% CP) would be preferred. The proportion of ${\alpha}$-amino N recovered by PDV relative to the intestinal N disappearance may vary with energy intake level of mixed forage diets.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2006년도 학술발표회 논문집
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• pp.115-119
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• 2006

The goal of this research is to develop and apply the generalized regression neural networks model (GRNNM) embedding genetic algorithm (GA) for pan evaporation, which is missed or ungaged and for the alfalfa reference evapotranspiration, which is not measured in South Korea. The GRNNM-GA is evaluated using the training, the testing, and reproduction performance respectively for the estimation of the PE and the alfalfa reference evapotranspiration. Since the observed data of the alfalfa reference evapotranspiration using lysimeter have not been measured for a long time in South Korea, the PM method is used to assume and estimate the observed alfalfa reference evapotranspiration. From this research, we evaluate the impact of the limited climatical variables on the accuracy of the GRNNM-GA. We should, furthermore, construct the credible data of the PE and the alfalfa reference evapotranspiration and suggest the reference data for irrigation and drainage networks system in South Korea.

• 한국초지조사료학회지
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• 제13권4호
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• pp.274-277
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• 1993

본 시험은 석회(0, 250, 500, 1,000kg/10a)와 인산(0, 17, 34kg/10a) 시용이 alfalfa(Medicago sativa L.)의 생장 및 질소고정에 미치는 영향을 구명하기 위해 전남대학교 농과대학내 온실에서 pot로 수행되었다. 파종후 9과에 alfalfa의 지상부와 지하부의 건물중 및 아세틸렌 환원력에 있어서 석회 및 인산시용효과가 나타났으며(p<0.05). 파종후 14과(개화초기)에는 alfalfa의 지상부와 지하부의 건물중에 있어서 석회시용 효과는 인정되지 않았으나 인산시용이 증가함에 따라서 건물중은 증가하였다(p<0.01). 또한 석회 및 인산시용이 증가함에 따라서 아세틸렌 환원력은 유의적으로 증가하였다(p<0.05). 파종후 9과에서 전 질소함량은 석회 및 인산 시용구에서 무시용구보다 감소되었으며(p<0.05), 파종후 14과에서는 석회 시용구는 무시용구보다 전 질소함량이 증가되었으나 인산 시용구는 무시용구 보다 낮아지는 경향을 보였다(p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.649-660
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• 2005

The effects of Lactobacillus buchneri 40788 and buffered propionic acid on preservation, intake and digestibility of alfalfa (Medicago sativa) and timothy (Phleum pratense) hay were investigated. During baling, forages were treated with L. buchneri 40788 (1.2${\times}$10$^6$ CFU/g) as a liquid (LLB) or as a granular preparation (GLB), with buffered propionic acid (10 mL/kg, BPA), or left untreated (control). Triplicate 500 kg round bales of each treatment were put up at two moisture levels for each forage: 17%${\pm}$0.33% and 20%${\pm}$0.30% for timothy and 17%${\pm}$0.20% and 19%${\pm}$0.27% for alfalfa (mean${\pm}$SD). Bales were sampled for chemical and microbiological analyses after 0, 30 and 60 d of storage. Compared to controls, all preservatives reduced (p<0.05) heating of both forages at all moisture levels with the exception of alfalfa baled at 19% moisture. After 60 d of storage, GLB reduced (p<0.05) moulds in 17% timothy hay as compared to other treatments, but at 20% moisture, moulds were reduced in LLB- and BPA-treated timothy as compared to controls. In alfalfa at 17% moisture, total bacteria were lower (p<0.05) in GLB-treated bales than LLB or control bales, but yeast and total bacteria were only reduced in BPA-treated alfalfa at 19% moisture. In situ DM disappearance of timothy (both moisture levels) and alfalfa (19% moisture level) increased (p<0.05) with LLB treatment compared to control. Digestibility of both forages did not differ (p>0.05) among treatments, however, voluntary DM intake of LLB-treated timothy (1.32 kg/d) was 22.3% higher (p<0.05) than control, and 14.1% higher than BPA-treated timothy. Treating timothy and alfalfa hay with L. buchneri 40788 or buffered propionic acid may improve the nutritive value of the hay when baled at 17 to 20% moisture.

• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.929-934
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• 2000

Optimum stand density of alfalfa (Medicago sativa L.) varies with locations and climates. Stand density is one of the factors that determines herbage yield, forage quality and persistence of alfalfa. As establishment costs increase, the question arises whether present population densities are optimum for obtaining maximum herbage yield and forage quality. The objectives of this study were: 1) to determine the optimum plant density for highest herbage yield and forage quality for the dehydrated alfalfa industry under Edmontons climatic conditions in Alberta, Canada; 2) to compare herbage yield and forage quality of the cultivars 'Algonquin' and 'Vernal' grown at a range of stand densities. Alfalfa seedlings of both cultivars were either transplanted at spacings of 6, 10, 15 and 25 cm or direct seeded at the 4.5 cm plant spacings, providing population densities of 494, 278, 100, 45 and $16plants/m^2$. The experimental design was a randomized complete block with a split-plot arrangement having three replicates; the main plots consisted of two alfalfa cultivars Algonquin and Vernal, and the sub-plots were the five population densities. The cultivar Vernal had significantly higher annual yield than did the cultivar Algonquin. There was no significant effect of plant density on herbage yield. There was no difference in crude protein (CP) between the two cultivars. At the first cut, there was a significant quadratic effect of plant density on CP content and the greatest CP occurred at the 100 plants/m2 density. Crude protein was not affected by plant density at the second cut. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) were not affected by plant density. The cultivar Algonquin usually had a lower ADF and NDF than cultivar Vernal. In conclusion, high population densities ($278plants/m^2$ or more) of alfalfa did not improve herbage yield and forage quality compared with low plant population densities ($100plants/m^2$ or less) of alfalfa.