• 한국작물학회지
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• 제49권1호
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• pp.7-11
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• 2004

Alfalfa (Medicago sativa L.) plants have been reported to be autotoxic as well as allelopathic. Laboratory and greenhouse experiments through petri-dish and pot test were conducted to determine autotoxic effects of alfalfa leaf and soil extracts on the germination or early seedling growth of alfalfa, and to evaluate allelopathic effects of alfalfa leaf residues on alfalfa, barnyard grass, com, eclipta and soybean. Alfalfa seed germination was delayed depending on aqueous extract concentration, with no difference in final germination after 48 hours. Alfalfa root length was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Root growth of alfalfa was significantly inhibited at extract concentration of more than 1 g dry tissue/L (g $\textrm{L}^{-1}$). Hypocotyl growth, however, was not affected by all the concentrations of leaf extracts. Soil extracts from 4-yr-old alfalfa stand significantly reduced alfalfa root length by 66%, while soil extracts from 0,1, and 3yr-old stand stimulated root length up to 14-32% over the control. Residue incorporation with dry matters of alfalfa leaf at 100 g $\textrm{kg}^{-1}$ reduced seedling length of several crop and weed species, ranging from 53 to 87% inhibition. Addition of nutrient solution into alfalfa leaf extracts alleviated alfalfa autotoxic effect. This result indicates alfalfa leaf and soil extracts or residues could exert autotoxic as well as allelopathic substances into soil environments during and after establishment.

• Journal of Animal Science and Technology
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• 제63권2호
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• pp.305-318
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• 2021

Alfalfa (Medicago sativa L.) is an important forage legume grown in Kansas, USA and its productivity with cool-season grasses however is unknown. The objective of this study was to determine the dry matter yield (DMY) and forage nutritive value of alfalfa-grass mixtures compared to those of alfalfa and grasses grown in monoculture with and without nitrogen fertilization. Three different alfalfa varieties were planted (reduced-lignin alfalfa, Roundup Ready, and conventional alfalfa) and two kinds of cool-season grasses (smooth brome, Bromus inermis Leyss, and tall fescue, Festuca arundinacea Schreb) were planted as a monoculture or in alfalfa-grass mixtures. Nitrogen fertilizer (urea) was applied at green-up at a rate of 56 kg/ha and after the second cutting at a rate of 56 kg/ha in 2016 and 2017, respectively. and control treatments received no nitrogen. DMY was significantly higher in monoculture alfalfa and alfalfa-grass mixtures than in grass monocultures. Between alfalfa monoculture and alfalfa-grass mixtures, no significant differences in DMY were found. For all treatments, nitrogen application significantly increased DMY compared to the control. In 2016 and 2017, the low-lignin alfalfa monoculture had the lowest acid detergent fiber (ADF) and the grass monocultures had the highest ADF. In 2016 and 2017, neutral detergent fiber (NDF) in smooth bromegrass and tall fescue was higher than in other species treatments. A low-lignin alfalfa monoculture had significantly lower NDF concentration compared to alfalfa-grass mixtures. When averaged over 2016 and 2017, relative feed value (RFV) was highest in low-lignin alfalfa and lowest in the grass monocultures. In both years, nitrogen fertilizer application did not affect nutritive values.

• 한국가금학회지
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• 제19권3호
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• pp.125-136
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• 1992

본 연구는 alfalfa meal의 첨가가 산란계의 생산성, 난황 착색도, 난황 cholesterol 함량 등에 미치는 영향을 규명하기 위하여 실시하였다. alfalfa meal을 밀기울와 대체하여 0, 3.5, 7% 첨가하는 3개 처리에 총192수의 갈색 산란계를 공식하였고, 공시계는 완전임의 배치하여 철제 cage에서 사육하였으며 1991년 7월23일부터 10월 7일까지 10주간에 걸쳐 시험을 진행하였다. 본 시험의 결과를 요약하면 다음과 같다. 1. 전 기간을 통해 산란율은 alfalfa meal 3.5% 첨가구에서 가장 높았으나 통계적인 유의차는 없었다. 2. 난중은 alfalfa meal 3.5, 7% 첨가구가 대조구에 비하여 유의하게 증가하였다(P<0.01). 3. 통계적인 유의차는 인정되지 않았으나 일산란양은 alfalfa meal 3.5% 첨가구에서 가장 높았다. 4. 1일 사료 섭취양은 alfalfa meal 3.5% 첨가시 가장 많았으나 사료 요구율은 가장 낮았다. 5. 산란 kg당 사료비는 alfalfa meal 3.5% 첨가구가 유의하게 타구에 비해 낮았다(P<0.05). 6. 난질 및 난각질에 있어서 난각후도와 난각중은 alfalfa meal의 첨가 수준이 증가함에 따라 증가하였으나 통계적인 유의차는 인정되지 않았다. 7. 난황 착색도는 alfalfa meal의 첨가량이 증가할 때 유의적으로 증가하였다(P<0.01). 8. 통계적인 유의차는 없었으나 난황 cholesterol 함양은 밀기울만을 급여한 구와 비교해서 alfalfa meal을 3.5%, 7% 급여한 구에서 감소하는 경향을 보였다.

• 한국작물학회지
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• 제39권3호
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• pp.295-305
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• 1994

본 실험은 alfalfa 와 vetch의 추출물과 잔유물의 콩과 옥수수에 대한 타감작용을 검정하기 위해서 실시 되었다. 콩과 옥수수에 대한 타감작용을 검정하기 위해서 영양생장기 때에 수확된 alfalfa 와 vetch를 건조시켜 추출물을 얻었고 또한 이들 잔기를 silica sand와 혼합처리하여 콩과 옥수수의 생육을 검토 하였고, 콩에 대해서는 근류균의 착생정도를 조사 하였다. 1. 발아시험에서는 롱과 옥수수의 발아율, 유근의 생장, 무게 등이 추출물 농도가 증가할수록 억제 되었으며, 최고 억제정도는 추출물 농도 20%에서 발생되었다. 추출물에 대하여 콩이 옥수수보다 더 민감하게 반응하였으며, alfalfa의 추출물이 vetch의 추출물보다 더 억제적이었다. 2. Alfalfa와 vetch를 silica sand에 혼합하여 콩과 옥수수의 간장, 엽면적, 지상부의 생육정도를 비교하였을 때 콩의 간장, 업면적, 지상부 생육은 alfalfa처리에서 더 억제적 이었으며, 옥수수의 생육은 vetch 처리에서 촉진 되었다. 3. Alfalfa와 vetch처리가 모두 콩 뿌리혹의 형성을 억제시켰는데 alfalfa처리가 vetch의 처리보다 뿌리혹의 형성을 더 억제 시켰다. 4. Alfalfa와 vetch의 추출물과 잔기의 처리는 옥수수보다는 콩의 발아와 생육에 더 억제적으로 작용하므로 콩 재배의 경우에는 이들 잔기의 관리가 필요하다고 생각되어진다.

• 한국초지조사료학회지
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• 제15권4호
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• pp.291-296
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• 1995

This study was conducted to determine the influence of alfalfa supplementation on intake, digestibility nitrogen and energy utilization in Korean native goats fed oak browse. This experiment was conducted by total collection method in laboratory, 1994. Diets included 100% oak bmwse(control), 75% oak browse+ 25% alfalfa and 50% oak browse + 50% alfalfa. Alfalfa supplemented diets were slightly higher CP(PO.O5). lignin (P<0.05) and tannin(P<0.05) contents than those control diets. Increasing levels of alfalfa supplementation, dry matter intake slightly increased, but no differences were observed in all diets. Digestibility for dry matter and cellular constituents showed higher for alfalfa supplemented diets than control diets(P<0.05), but NDF and ADF digestibility were similar for all diets. Alfalfa supplemented diets furnished higher dietary N, apparently digested N and retained N than did the control diets(P<0.05). Consumed energy slightly increased with increasing levels of alfalfa supplementation(P>0.05). DE was high(P<0.05) in alfalfa supplemented diets. but ME was similar for all diets. As above results, oak browse diets alone did not supply N and energy in excess of NRC recommended allowance for goats. Hence, goats fed oak browse with supplementation of protein sources such as alfalfa will be need to provides adequate nutrients.

• 한국초지조사료학회지
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• 제14권2호
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• pp.88-92
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• 1994

본 시험은 석회(0, 250, 1,000kg/10a)와 인산(0, 17 및 34kg/10a)t용이 alfalfa의 생장 및 질소고정에 미치는 영향을 구명하기 위하여 전남대학교 농과대학내 온실에서 pot로 수행되었는데 그 결과를 요약하면 다음과 같다. 실험기간동안 alfalfa의 지상부와 지하부의 건물중, 근류중량 및 acetylene환원력은 pH6.2인 토양에서 석회시용 효과가 인정되지 않았지만, 인산시용 효과는 인정되었다(p<0.05). 파종 후 7주에서 alfalfa의 부위별 전질소 함량은 석회시용구가 무시용구보다 증가하였으나 석회시용 수준간에는 차이가 나타나지 않았으며 인산시용 효과도 나타나지 않았다. 파종 후 12주에서 alfalfa의 부위별 전질소 함량은 석회시용구와 무시용구사이에 차이가 나타나지 않았고, 인산시용구는 무시용구보다 낮아지는 경향을 보였다(p<0.05). 이상의 결과를 종합해볼 때, pH6.2인 약산성 토양에서 석회 10a당 250kg시용구아ㅗ 인산 34kg시용구가 alfalfa의 생장 및 질소 고정에 유리하게 작용하였다.

• Journal of Animal Science and Technology
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• 제63권4호
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• pp.799-814
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• 2021

Information is lacking about the effect of date of a fall cut of alfalfa (Medicago sativa L.) on dry matter yield (DMY), forage nutritive value, and stands persistence. The objective of this study was to determine the effect of timing of a fall cut on DMY, forage nutritive value and stand persistence of three alfalfa varieties: low-lignin Hi-Gest 360, Roundup Ready Tonica, and conventional Gunner in Northeastern Kansas in the United States. The field study was carried out by splitting plot in randomized complete block design with four replications. The harvesting data of different maturity stages were collected in each year from 2015 to 2018. Three cuts were harvested based on the stage of maturity, and the last (fourth) cut was done on September 15, September 30, October 15, and October 30 of each year. The persistence of the alfalfa stands was determined each fall after the last cut, and each spring after the first cut, by counting the number of live plants in a randomly placed quadrat in each plot. Alfalfa cut on September 15 and September 30 had a higher stand persistence compared to alfalfa cut on October 15 and October 30. The DMY of the first cut in 2016 was significantly higher in roundup ready than the low-lignin alfalfa variety. In the second cut, DMY was significantly higher in conventional alfalfa than the roundup ready. There were no significant differences in DMY between alfalfa varieties in the rest of seasonal cuttings in 2016 and 2017 and annual total yield in both years. In general, low lignin alfalfa variety had higher crude protein and relative feed value and lower acid detergent fiber and neutral detergent fiber contents than those in roundup ready and conventional alfalfa varieties. On average, nutritive value of alfalfa was generally affected by last cutting dates in 2017. Based on 3-year data the last cutting of alfalfa in the fall could be done by September 30-October 15 without harmful effect on DMY.

• 한국작물학회지
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• 제45권5호
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• pp.310-315
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• 2000

Alfalfa (Medicago sativa L.) plants have been reported to contain water-soluble substances that are autotoxic as well as allelopathic. Laboratory experiment through a petri-dish assay with imbibed seeds was conducted to evaluate both autotoxic and allelopathic effects of alfalfa leaf extracts on the germination and early seedling growth of alfalfa, red clover, crested wheatgrass, and Russian wildrye. Alfalfa seed germination was delayed dependent on extract concentration, with no difference in final germination at 72 hours. Root growth of alfalfa was stimulated up to 14% above control at very low concentrations of both leaf and stem extracts of alfalfa and was significantly reduced at extract concentration of more than 0.5g dry tissue/L (${gL}_{-1}$). Leaf extracts were generally more autotoxic for root growth than were stem extracts. Hypocotyl growth was not affected by all the concentrations of both leaf and stem extracts. Root length of legumes was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Hypocotyl growth of two legume plants and plant height of two grasses were not influenced by extracts. Seed germination and root growth of legumes were more inhibited by aqueous extracts of alfalfa leaf than were those of grasses. This result indicates autotoxic effect of alfalfa leaf extracts seems to be greater than allelopathic effect.

• 한국작물학회지
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• 제45권2호
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• pp.134-138
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• 2000

Most parts of alfalfa plant have been reported to contain autotoxic substances that inhibit seed germination and early seedling growth, however, the chemical(s) is not still studied much. Effect of seed leachates of 'Vernal' alfalfa (Medicago sativa L.) was evaluated for inhibition of alfalfa germination and root growth through bioassay. Alfalfa seeds were extracted in 1 L deionized water for 1 h after soaking and the leachates caused to reduce root length of alfalfa significantly as the soaking time increased. Crude seeds at 4 g L$L^{-1}$ exudated autotoxins that reduce significantly root length by 34 % compared to the control, when the seeds soaked in deionized water for 24 h. However, the extracts did not affect final germination as well as speed of germination. Extracts from ground seeds significantly reduced speed of germination (GT 50) and root length. The results indicate that release of autotoxic substances from seeds during seed imbibition was increased with increase of soaking time and seed amount, and that autotoxicity was more occurred in ground seeds than in crude seeds.

• 한국초지조사료학회지
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• 제20권1호
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• pp.13-18
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• 2000

An efficient method for Agrobacterium-mediated transformation of forage crop alfalfa (Medicago sativa L.) was established using secondary somatic embryogenic calli. Agrobacterium tumefaciens strain EHAlOl and a binary vector pIG121-Hm which has selection markers for kanamycin and hygromycin have been shown to be an efticient materials for alfalfa transformation. The secondary somatic embryogenic calli originated from hypocotyl explants of alfalfa were efficient infection materials for Agrobacterium EHAlOl and normally germinated into plantlets. The introduced gene (GUS) was constitutively expressed in all tissues of transgenic alfalfa with different expression levels. These results indicate that the use of pIG121-Hm vector, Agrobacterium EHAlOl and improved culture system of callus facilitate the transformation of alfalfa. (Key words : Agrobacterium, Alfalfa, Gene transfer, Transformation)