• 한국치위생학회지
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• 제12권1호
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• pp.1-15
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• 2012

Objectives : The purpose of this study was to examine the effect between job stress in jobholders and xerostomia. Methods : 250 jobholders living in Jecheon city were the subjects of this questionnaire. The questionnaire was made up of three contents and 37 items: general characteristic(13), job stress(14), degree & behavior of xerostomia(10). The data were analyzed by two-sample t-test, one-way ANOVA to examine the subjects general characteristics, job stress and degree of xerostomia and were analyzed by Chi-square test to examine the subjects general characteristics, job stress and behavior of xerostomia. Results : Only 215 jobholders were evaluated due to inadequate responses. The results were as follow. 1. As general characteristic of jobholder, male(83.7%) were more than women(16.3%), 30~39 year-old(47%) in age variable, university graduation(63.7%) in the last educational background variable, 2~3 million won(31.2%) in the month average income variable, 1~5 year(33.5%) in tour of duty variable, non-smoker(47.9%) in smoking variable were most. Married(58.6%) were more than unmarried(39.5%). Alcoholic(69.8%) were more than non-alcoholic(30.2%). 2. As classification of job stress, high strain group was 28.4%, active group was 26%, low strain group was 24.2%, passive group was 21.4%. 3. Analysis of effect between general characteristic and degree & behavior of xerostomia showed smoker were statistical significantly higher than non-smoker on 'dry eat', 'Am-sal', 'Night awake', 'Slip-liq'and 'Gumcandy'(p<0.05) and showed alcoholic were statistical significantly higher than non-alcoholic on 'Dry PM', 'Night awake, $H_2O$-bed'(p<0.05). 4. Analysis of effect between job stress and degree & behavior of xerostomia showed hight strain group were statistical significantly higher than low strain group on 'Dry PM', 'Dry-day', 'Am-sal', 'Eff-life'and 'Night awake'(p<0.05). Conclusions : As high strain group were higher than other groups on degree & behavior of xerostomia, stress would be factor that have an effect on xerostomia. Thus consider and management of stress is necessary for diagnosis and treatment of xerostomia.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.60-60
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• 2021

Non-alcoholic fatty liver disease (NAFLD), especially including non-alcoholic steatohepatitis (NASH) is one of the common diseases with 25% of prevalence globally, but there is no thera-peutic access available. Amomum villosum var. xanthioides (Wall. ex Baker) T.L.Wu & S.J.Chen (AX), which is a medicinal herb and traditionally used for treating digestive tract disorders in Asia countries. We aimed to examine pharmacological effects of ethyl acetate fraction of AX (AXEF) against ER stress-induced NASH mice model using C57/BL6J male mice by tunicamycin (TM, 2 mg/kg) injection focusing on the oxidative stress. Mice were orally administrated AXEF (12.5, 25, or 50 mg/kg), silymarin (50 mg/kg) or distilled water daily for 5 days, and outcomes for fatty liver, inflammation, and oxidative stress were measured in serum or liver tissue levels. AXEF drastically attenuated hepatic ER stress-induced NASH which were evidenced by decreases of li-pid droplet accumulations, serum liver enzymes, hepatic inflammations, and cell death signals in the hepatic tissue or serum levels. Interestingly, AXEF showed potent antioxidant effects by quenching of reactive oxidative stress and its final product of lipid peroxide in the hepatic tissue, specifically increase of metallothionein (MT). To confirm underlying actions of AXEF, we ob-served that AXEF increase MT1gene promoter activities in the physiological levels. Collectively, AXEF showed antioxidant properties on TM-induced ER stress of NASH by enhancement of MTs.

• 대한한의학방제학회지
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• 제17권2호
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• pp.163-174
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• 2009

Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.

• 대한예방한의학회지
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• 제8권1호
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• pp.109-114
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• 2004

Oxidative stress is one of the major reasons for brain aging and neurodegeneration. Ethanol and acetaldehyde increase the levle of oxidative stress in brain tissue resulting in aging and neurodegeneration related alcoholic dementia. Ginkgo biloba extracts are used as therapeutic and preventive agent for dementia. Here, it was investigated whether Ginkgo biloba extract show the effectiveness against ethanol- and acetaldehyde-induced oxidative stress in rat brain. Ethanol and acetaldehyde increased the level of oxidative stress by about 35% to 50% in rat brain tissue. However, Ginkgo biloba extracts reduced the level of ethanol- and acetaldehyde-induced oxidative stress. This result might reveal the link between the effectiveness of Ginkgo biloba extracts on oxidative stress and its effectiveness on alcoholic dementia.

• Journal of Ginseng Research
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• 제39권2호
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• pp.105-115
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• 2015

Background: Alcoholic steatosis is the earliest and most common liver disease, and may precede the onset of more severe forms of liver injury. Methods: The effect of Korean Red Ginseng extract (RGE) was tested in two murine models of ethanol (EtOH)-feeding and EtOH-treated hepatocytes. Results: Blood biochemistry analysis demonstrated that RGE treatment improved liver function. Histopathology and measurement of hepatic triglyceride content verified the ability of RGE to inhibit fat accumulation. Consistent with this, RGE administration downregulated hepatic lipogenic gene induction and restored hepatic lipolytic gene repression by EtOH. The role of oxidative stress in the pathogenesis of alcoholic liver diseases is well established. Treatment with RGE attenuated EtOH-induced cytochrome P450 2E1, 4-hydroxynonenal, and nitrotyrosine levels. Alcohol consumption also decreased phosphorylation of adenosine monophosphate-activated protein kinase, which was restored by RGE. Moreover, RGE markedly inhibited fat accumulation in EtOH-treated hepatocytes, which correlated with a decrease in sterol regulatory element-binding protein-1 and a commensurate increase in sirtuin 1 and peroxisome proliferator-activated receptor-a expression. Interestingly, the ginsenosides Rb2 and Rd, but not Rb1, significantly inhibited fat accumulation in hepatocytes. Conclusion: These results demonstrate that RGE and its ginsenoside components inhibit alcoholic steatosis and liver injury by adenosine monophosphate-activated protein kinase/sirtuin 1 activation both in vivo and in vitro, suggesting that RGE may have a potential to treat alcoholic liver disease.

• 대한한방내과학회지
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• 제28권1호
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• pp.115-123
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• 2007

Objectives : To investigate the correlations among alcohol consumption, alcoholic liver disorders, physical symptoms, and behaviors in heavy drinkers. Methods : 53 males who self-realized their severe alcohol consumption were enrolled in this study. 10 answers for a questionnaire, serum parameter, sonographic finding and body mass index were attained. The correlations between them were analyzed using Pearson's correlation and Student's t-test. Results : The average consumption of alcohol in these subjects was 2.5-fold over social drinkers. The incidence of alcoholic hepatitis was around 30%, while fatty liver 73%, and abnormal GGT 77%, respectively. No specific correlation between average volume of daily alcoholic consumption and alcohol-related hepatic parameters was shown in this study, but correlative tendency between fatty liver and body mass index was exhibited. Conclusions : This study may indicate that alcoholic liver injuries are caused by not just volume of alcohol consumed but more mixed factors including inherited genetic components, body fat mass, foods and other physical or emotional stress.

• 한국축산식품학회지
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• 제37권6호
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• pp.931-939
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• 2017

Alcoholic liver disease (ALD) is a complex multifaceted disease that involves oxidative stress and inflammation as the key mediators. Despite decades of intensive research, there are no FDA-approved therapies, and/or no effective cure is yet available. Probiotics have received increasing attention in the past few years due to their well-documented gastrointestinal health-promoting effects. Interestingly, emerging studies have suggested that certain probiotics may offer benefits beyond the gut. Lactobacillus fermentum LA12 has been previously demonstrated to play a role in inflammatory-related disease. However, the possible protective effect of L. fermentum LA12 on ALD still remain to be explored. Thus, the aim of this study was to evaluate the possible protective effect of L. fermentum LA12 on alcohol-induced gut barrier dysfunction and liver damage in a rat model of alcoholic steatohepatitis (ASH). Daily oral administration of L. fermentum LA12 in rat model of ASH for four weeks was shown to significantly reduced intestinal nitric oxide production and hyperpermeability. Moreover, small intestinal histological- and qRT-PCR analysis further revealed that L. fermentum LA12 treatment was capable of up-regulating the mRNA expression levels of tight junction proteins, thereby stimulating the restitution of barrier structure and function. Serum and hepatic analyses also revealed that the restoration of epithelial barrier function may prevent the leakage of endotoxin into the blood, subsequently improve liver function and hepatic steatosis in the L. fermentum LA12-treated rats. Altogether, results in this study suggest that L. fermentum LA12 may be used as a dietary adjunct for the prevention and treatment of ASH.

• 한국인구학
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• 제26권2호
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• pp.91-110
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• 2003

고위험 음주는 개인 건강 뿐 아니라 사회에 막대한 부담을 초래한다. 본 연구는 보건복지부와 서울대학교외 건강위험요인 전국조사 자료에 중도 절단 이변량 프로빗 모형(bivariate probit model with censoring)을 활용하여 한국 사회에서 15세 이상 인구계층의 고위험 음주에 미치는 요인을 규명하였다. 연구결과에 따르면 우리나라에서 소주가 음주 주종 중 고위험 음주와 가장 관련성이 큰 주종임이 밝혀졌다. 그동안 소주의 문제점에 관해서는 대부분 인식하고 있었으나 전국 조사 자료 및 최신 통계분석방법을 적용한 연구가 전무하여 실증적인 뒷받침이 되지 않았던 사실이 밝혀진 셈이다. 또한 나이가 많은 계층, 배우자와 동거하지 않는 계층, 경제활동에 종사하는 계층, 스트레스에 취약한 계층, 다양한 주종을 소비하는 계층이 각각 상대적으로 그렇지 않는 계층에 비해 고위험 음주를 할 가능성이 높았다. 본 연구결과로써 한국 사회에서 음주로 인한 위해를 감소시키기 위해서는 주종별로 차별화된 정책과 함께 고위험 음주 가능성이 상대적으로 높은 인구계층을 대상으로 정부 및 민간 노력이 집중되어야 한다는 정책적 시사점을 도출할 수 있었다.

• 약학회지
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• 제56권4호
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• pp.260-267
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• 2012

This study examined the effects of baicalin, a bioactive flavonoid isolated from Scutellaria baicalensis, on hepatic injury caused by ischemia/reperfusion (I/R) in alcoholic fatty liver. Rats were fed an ethanol liquid diet or a control isocaloric diet for 5 weeks, and then subjected to 60 min of hepatic ischemia and 5 h of reperfusion. Baicalin (200 mg/kg) was administered intraperitoneally 24 and 1 h before ischemia. After reperfusion, baicalin attenuated the increase in serum alanine aminotransferase activity. The levels of cytosolic cytochrome c protein expression, caspase-3 activity, the number of apoptotic cells increased after reperfusion, which were higher in ethanol-fed animals, were attenuated by baicalin. Following I/R, the hepatic lipid peroxidation was elevated, whereas hepatic glutathione content was decreased. These changes attenuated by baicalin. In ethanol-fed animals, baicalin augmented the increases in heme oxygenase-1 protein and mRNA expressions, and nuclear Nrf2 expression. In conclusion, our findings suggest that baicalin ameliorates I/R-induced hepatocellular damage by suppressing apoptosis and oxidative stress in alcoholic fatty liver.

• 대한한의학방제학회지
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• 제28권2호
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• pp.189-198
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• 2020

Objectives : This study investigated the liver-protective effects of MJY2018, a Herbal formula, against alcoholic fatty liver disease and anti-oxidative effects. Methods : Its effects were investigated in an alcoholic fatty liver disease model in male C57BL/6 mice, which were fed Lieber-DeCarli liquid diet containing ethanol. MJY2018 (100 and 200 mg/kg bw/day) or silymarin (50 mg/kg bw/day) were orally administered daily in the alcoholic fatty liver disease mice for 16 days. Results : The results indicate that MJY2018 promotes hepatoprotection by significantly reducing aspartate transaminase (AST) and alanine transaminase (ALT) levels as indicators of liver damage in the serum. Furthermore, MJY2018 reduced accumulation of triglyceride and total cholesterol, increased levels of superoxide dismutase (SOD) and glutathione (GSH) in the livers of the alcoholic fatty liver disease mice model. Additionally, it improved the serum alcohol dehydrogenase (ADH) activity. Conclusions : These results indicate that MJY2018 were effective in improving and protecting oxidative stress and alcoholic liver disease.