• 제목/요약/키워드: alcA

검색결과 153건 처리시간 0.024초

Xylose 발효효모의 분리 및 성질 (Isolation and Identification of Xylose fermenting Yeast)

  • 김남순;서정훈
    • 한국미생물·생명공학회지
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    • 제16권6호
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    • pp.505-509
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    • 1988
  • Xylose 발효효모를 토양에서 분리하였으며 (X-6-41) 이는 Lodder씨 분류기준에 따라 Candida 속으로 동정되었다. 삼각플라스크 정치배양법에 의한 xylose 발효시 2% xylose, N원 asparagine, pH 4에서 발효율 약 80%로 나타났고 Mini-Jar for mentor를 이용하여 glucose와 xylose를 1 : 1로 혼합한 총당 5% 기질에서 발효를 시켜 본 바 발효율은 총당에 대해 약 72.3%(alcohol 2.3%(v/v)였다. 본 균주에 의한 xylose 발효는 일반적으로 보고된 바와 같이 호기적 발효과정이였으며 glucose와 xylose 혼합기질의 경우 glucose가 먼저 이용되고 xylose가 나중에 이용되는 것으로 나타났다. 5% xylose를 기질로 하여 Mini-Jar fermentor에서 발효시켰을 때 최대 발효율 69%(alcohol 2.2%(v/v)로 나타났으며 발효중에 xylitol이 생성되었다.

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Controlled Expression and Secretion of Aspergillus oryzae Alkaline Protease in Aspergillus nidulans

  • Kim, Eun-Ah;Lee, Jeong-Goo;Whang, Mi-Kyung;Park, Hee-Moon;Kim, Jeong-Yoon;Chae, Suhn-Kee;Maeng, Pil-Jae
    • Journal of Microbiology
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    • 제39권2호
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    • pp.95-101
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    • 2001
  • In an effort to develop an efficient expression and secretion system for heterologous proteins in Aspergilius nidulans, the PCR-amplified coding sequence for alkaline pretense (AlpA) of A. oryzae was cloned into a fungal expression vector downstream of A. nidulans aicA (alcohol dehydrogenase) promoter to yield pRAAlp. Transformation of A. nidulans with pRAAlp gave stable transformants harboring various copy numbers (3 to 10) of integrated alpA gene, from among which 6 representatives were selected. On a medium containing 0.8% ammonium sulfate that represses the expression of the host's own pretense, the alcA prumoter-controlled AlpA expression was strongly induced by threonine but repressed by glucose. The level of AlpA secretion was highest (approximately 666 mU/ml) in transformant ALP6 containing the largest copy number integrated alpA. However, the level of AlpA secretion was not necessarily proportional to the copy numbers of the integrated alpA genes. The N-terminal sequence or the secreted mature AlpA was determined to be Gly-Leu-Thr-Thr-Gln-Lys-Ser and its molecular mass to be approximately 34 kDa, indicating that AlpA is properly processed by the removal of 121 N-terminal amino acids.

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ITO와 IZO 타겟의 Co-sputtering 방법으로 성장시킨 IZTO 박막의 전기적 광학적 구조적 특성연구 (Electrical, optical, and structural properties of IZTO films grown by co-sputtering method using ITO and IZO target)

  • 정진아;최광혁;문종민;배정혁;김한기
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2007년도 하계학술대회 논문집 Vol.8
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    • pp.379-380
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    • 2007
  • The characteristics of a co-sputtered indium zinc tin oxide (IZTO) films prepared by dual target dc magnetron sputtering from IZO and ITO targets at a room temperature are investigated. Film properties, such as sheet resistance, optical transmittance, surface work function and surface roughness were examined as a function of ITO dc power at constant IZO dc power of 100 W. It was shown that the increase of the ITO dc power during co-sputtering of ITO and IZO target resulted in an increase of sheet resistance of the IZTO films. This can be attributed to high resistivity of ITO film prepared at room temperature. Surface smoothness and roughness were investigated by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The synchrotron x-ray scattering results obtained from IZTO film with different ITO contents showed that introduction of ITO atoms into amorphous IZO film resulted in a crystallization of IZTO film with (222) preferred orientation due to low alc transition temperature of ITO film. However, the transmittance of the IZTO films with thickness of 150 nm is between 80 and 85 % at wavelength of 550 nm regardless of ITO content. Possible mechanism to explain the ITO and IZO co-sputtering effect on properties of IZTO is suggested.

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건조수축에 따른 균열 억제를 위한 규사 혼입 CLC의 특성 (Properties of CLC using Silica to Suppress Cracking due to Drying Shrinkage)

  • 이창우;이상수
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2021년도 가을 학술논문 발표대회
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    • pp.125-126
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    • 2021
  • In order to improve the housing culture, construction changes for the utilization of diverse and multifunctional spaces are appearing in response to the increasing diverse needs of consumers. Cellular Light-weight Concrete (CLC) is being developed for use in fire-resistant heat-insulating walls and non-bearing walls. However, manufacturing non-uniformity has become a problem as a drawback due to the use of foamed bubbles and normal temperature curing, and additional research is required. Therefore, in order to suppress cracks due to drying shrinkage, silica sand is mixed with CLC to try to understand its characteristics. In the experiment, the compressive strength from 7 to 28 days of age was measured via a constant temperature and humidity chamber, and the drying shrinkage was analyzed according to each condition using a strain gauge. The compressive strength of matrix tends to decrease as the substitution rate of silica sand increases. This is judged by the result derived from the fact that the specific surface area of silica sand is smaller than that of slag. Based on KS F 2701 (ALC block), the compressive strength of 0.6 products is 4.9 MPa or more as a guide, so the maximum replacement rate of silica sand that satisfies this can be seen at 60%. Looking at the change in drying shrinkage for just 7 days, the shrinkage due to temperature change and drying is 0.7 mm, and the possibility of cracking due to shrinkage can be seen, and it seems that continuous improvement and supplementation are needed in the future.

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이산화티탄 광촉매의 효율적 적용을 위한 LEFC 블록 제작에 관한 연구 (A Study on the Development of Light Emotion Friendly Concrete Block for Efficient Application of Titan-oxide Photocatalyst)

  • 김병일;오상근;서승훈
    • 한국구조물진단유지관리공학회 논문집
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    • 제23권6호
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    • pp.120-131
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    • 2019
  • 본 연구에서는 빛 감성친화형 콘크리트에 광촉매를 적용하여 대기질 및 실내공기질을 개선하기 위한 LEFC 블록을 개발하고자 하였다. LEFC에 광촉매를 적용하게 되면 자외선 입사면 반대편에서도 투과로 인한 자외선이 존재하여 광촉매가 반응함으로써 일반 건축 자재를 적용한 경우보다 광촉매 반응효율이 크게 상승한다. 따라서 광촉매를 LEFC에 적용하기 위해 슬럼프, J-ring, L-box 테스트를 통한 자기충전성능을 평가하여 최적 배합을 결정하였고, 압축 및 휨 강도 시험을 통해 역학성능을 평가하였다. 그리고 TiO2 분포도를 확인하기 위해 SEM과 EDS 분석을 실시하였다. ALC골재와 단열재 적용으로 광촉매 사용량을 줄이고 단위중량을 감소시키는 방안을 활용하여 광촉매 효율을 증가시키는 빛투과 콘크리트 블록을 제작하였고, 향후 건조수축 등의 문제점 개선 및 NOx 제거 실험을 통한 LEFC 블록 성능 평가를 진행하고자 한다.

한국산(韓國産) 식물식용유지(植物食用油脂)의 성분(成分)에 관(關)한 연구(硏究) -제3보(第3報) 해바라기 종자(種子)의 유성성분(油性成分)에 대하여- (Studies on the Constituents of Korean Edible Oils and Fats -Part 3. Studies on the oil soluble constituents of sunflower seed-)

  • 최기영;고영수
    • Journal of Nutrition and Health
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    • 제12권2호
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    • pp.75-85
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    • 1979
  • The Korean origin sunflower (Helianthus Annus Linn.) seed of netural lipid were analysed by thinchrography, High performance liquid chromatography, preparative Thinlayer and Gas liquid chromatography. 1) The seed oil triglyceride components were conveniently separated based on their degree of unsaturation by employing the chromatography on silica gel sintered rod impregnated with 12.5% silver nitrate. Sunflower seed oil was composed of triglyceride, especially trilinolein 57. 74% triolein 25.28%, tripalmitin 7 55% ana tristearin 9.43% by a thinctrography. 2) The fatty acid compositions of seed oil have been determined by a high performance liquid chromatographic analysis using a ALC/GPC 244 type from Waters Association (Japan) with ${\mu}$ Bondapak FFAA column. It contained stearic acid 8.59%, oleic acid 27. 19%, palmitic acid 7.50% and linoleic acid 56.72% respectively. 3) The composition of sterols were determined by a preparative Thinlayer and Gas liquid chromatographic analysis. It was noted that sitosterol was the major sterol in the Korean sunflower seed. The results showed that contents of sterols were cholesterol trace, campesterol $13_.^{22\sim}13.9%$, stigmasterol $13.8{\sim}14.1%$, If, sitosterol $58.4{\sim}60.7%$, ${\vartriangle}^7$-stigmastenol $10.2{\sim}10.5%$ and ${\vartriangle}^{7,24(25)}$-stigmastenol $3.6{\sim}3.8%$ by method of planimetry and triangulation.

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인슐린 비의존성 당뇨환자의 자기효능, 지식, 자기간호행위와 대사조절간의 관계 (A Study of the Relationship of Self-Efficacy, Knowledge, and Self-Care Behavior to Metabolic Level in Patients with NIDDM)

  • 이숙희
    • 기본간호학회지
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    • 제10권2호
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    • pp.171-180
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    • 2003
  • Purpose: The purpose of this study was to investigate the relationship of self-efficacy, knowledge about the disease, and self-care behavior to the metabolic level in patients with NIDDM. Method: Data were collected from March 15 to April 15, 2001. Participants in the study were 48 patients with NIDDM who were seen regularly at the endocrinology out-patient department of Gwangju Christian Hospital. The data were collected through individual interviews and patient blood samples. The data were analyzed with SPSS/PC+, using t-test, ANOVA, Pearson correlation coefficients and Cronbach's Alpha. Result: 1. The mean score for self-efficacy was $71.37{\pm}15.56$, knowledge $15.00{\pm}2.84$, self-care behavior $63.18{\pm}13.10$. The mean score for HbAlc level was $7.66{\pm}1.77%$, HDL cholesterol level, $46.22{\pm}13.04 mg/dl$ and total cholesterol level, $187.93{\pm}41.45 mg/dl$. 2. There was a significant difference between patients with a spouse and those without one (t=-2.08, p=0.042), in knowledge according to level of obesity (t=5.14, p=0.010), duration of illness (t=3.22, p=0.031) and presence of complications related to diabetes (t=-2.58, p= 0.013). There were no significant differences in self-care behavior, but there were significant differences in sugar metabolic level according to sex (t=-2.02, p=0.050). 3. The correlation between diabetic patients' self-efficacy, knowledge and self-care behaviour and metabolic control was significantly related only to self-efficacy and self-care behavior (r=.692, p=0.000). Conclusion: It is necessary to improve self-efficacy, knowledge, and self-care behavior for patients with NIDDM by using nursing intervention programs to promote and maintain metabolic control.

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해수오염원추적자로서의 플루오르화물이온 및 진해만의 플루오르화물이온농도분포 (Eligibility of Fluoride Ion as A Tracer of Wastewaters and Distribution of Fluoride in Jinhae Bay)

  • 원종훈;박길순
    • 한국해양학회지
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    • 제8권1호
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    • pp.9-21
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    • 1973
  • 1. 플루오르를 함유하는 배수가 바다에 유입되었을 때 플루오르화물이온을 그 추적자로 이용할 수 있다. 2. "Dotite"시약 "알풋손"에 의한 해수중의 플루오르 정량법을 검토하였다. 3. 진해만의 플루오르화물이온 농도분포를 조사하여 진해만에 플루오르를 함유하는 배수가 평균 최소한 0.13% 혼입되어 있으며 그것은 진해 모화학공장의 폐수에 기인한다. 그리고 그것이 혼입되어가는 경로를 밝혔다. 그것이 혼입되어가는 경로를 밝혔다.

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임베디드 소프트웨어 개발현장보안에 관한 연구 (A Study on the Development Site Security for Embedded Software)

  • 여상수;김태훈;조성언;코우이치 사쿠라이
    • 한국항행학회논문지
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    • 제11권3호
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    • pp.259-265
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    • 2007
  • 유비쿼터스 컴퓨팅을 구현하기 위한 임베디드 시스템적 요소, 즉 각종 전자기기, 가전제품, 제어장치 들은 단순히 회로로만 구성되어 있는 것이 아니라 특정한 기능을 수행하도록 프로그램이 내장되어 있는데 이러한 시스템 제어역할을 수행하는 다양한 프로그램을 임베디드 소프트웨어라고 정의할 수 있다. 임베디드 소프트웨어는 시스템의 기능을 제어하는 핵심 요소이므로 내용이나 제어 구조가 공개되는 경우에는 심각한 영향을 받을 수 있다. 임베디드 소프트웨어의 보안은 크게 두 부분으로 나뉘어 고려될 수 있다. 첫 번째는 외부의 위협원이 내부로 침투하여 관련 정보를 유출하는 것이고, 두 번째는 내부 혹은 외부의 위협원이 임베디드 소프트웨어에 악성 코드를 삽입하는 등 불법적인 행동을 하는 것이다. 본 논문에서는 첫 번째 관점에서, 임베디드 소프트웨어를 개발하고 있는 개발 현장에 대한 보안 점검 요소들을 도출하고 제안하였다. 개발 현장에 대한 보안 점검을 통해 외부 위협원의 접근을 원천적으로 차단하는 동시에, 임베디드 소프트웨어의 비인가된 변형을 간접적으로 예방할 수 있을 것으로 기대된다.

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여자노인의 영양상태 평가 및 건강관리를 위한 Nutritional Risk Index (NRI) 비교 분석 (The Analysis of Geriatric Nutritional Risk Index (GNRI) for Nutritional Assessment and Health Care in Elderly Women)

  • 양은주
    • Journal of Nutrition and Health
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    • 제42권3호
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    • pp.234-245
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    • 2009
  • Nutritional assessment for the elderly can identify health status and morbidity. However, development of Nutritional Risk Index (NRI) remains limited for elderly because of difficulties in understanding physiological mechanism of elderly. This study was performed to analyze and develop Nutritional Risk Index for Korean elderly Women (Geriatric Nutritional Risk Index, GNRI). Based on literature review, factors for NRI were identified and indices were assessed by a cross-sectional survey. The survey involved Korean elderly women (${\geq}$60, n = 94) in Gwangju area, and sociodemographics, lifestyle characteristics, health conditions, dietary intakes based on 24h- recall, anthropometric measures (wt, ht, BMI, waist, hip, WHR, body protein, body fat, abdominal fat, and triceps skinfold thickness), and clinical biochemistry parameters (systolic blood pressure, diastolic blood pressure, cholesterol, HDL-cholesterol, triglyceride, total protein, albumin, prealbumin, hemoglobin, hematocrit, fasting blood glucose, HbAlc, ferritin, Zn, Ca, Na, K, Vit E, Vit $B_{12}$, folate, C-reactive protein) were examined relation to nutritional risk index. Based on literature review and data analyses, three NRIs were categorized (NRI I, NRI II, NRI III) and used for further analysis. NRI I was related to having metabolic syndrome, NRI II was related to serum albumin and body weight, and NRI III was related to food habit and health concerns. Abdominal fat (%) of elderly was correlated with each NRIs. NRI II was correlated with nutritional deficiency and higher tendency of inflammatory response, and NRI III was correlated with nutritional status which tend to be lower on aging (protein, folate, Vit $B_{12}$). NRI can serve as a useful tools in assessing health risk and nutritional status. Some modification of items in NRI and validity study are need to apply to Korean elderly.